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Molecular Plant 2014年第8期7卷 1329-1349页

作者： Junjie Tan Zhenhua Tan Fuqing Wu peike Sheng Yueqin Heng Xinhua wang Yulong Ren Jiulin wang Xiuping Guo Xin Zhang Zhijun Cheng Ling Jiang Xuanming Liu Haiyang wang Jianmin Wan State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology Hunan University Changsha 410082 P.R. China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science Chinese Academy of AgriculturalSciences Beijing 100081 RR. China National Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 P.R. China

Pentatricopeptide repeat （PPR） proteins comprise a large family in higher plants and modulate organellar gene expression by participating in various aspects of organellar RNA metabolism. In rice, the family contains 477 members, and the majority of their functions remain unclear. In this study, we isolated and characterized a rice mutant, white stripe leaf （wsl）, which displays chlorotic striations early in development. Map-based cloning revealed that WSL encodes a newly identified rice PPR protein which targets the chloroplasts. In wsl mutants, PEP-dependent plastid gene expression was significantly down-regulated, and plastid rRNAs and translation products accumulate to very low levels. Consistently with the observations, wsl shows a strong defect in the splicing of chloroplast transcript rpl2, resulting in aberrant transcript accumulation and its product reduction in the mutant. The wsl shows enhanced sensitivity to ABA, salinity, and sugar, and it accumulates more H2O2 than wild-type. These results suggest the reduced translation efficiency may affect the response of the mutant to abiotic stress.

关键词： PPR protein WSL ABA chloroplast RNA splicing rpl2 abiotic stress responses.

A preliminary observation on rod cell photobiomodulation in treating diabetic macular edema

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Advances in Ophthalmology Practice and Research 2022年第2期2卷 30-35页

作者： Zhiqing Chen Binbin Chen peike Hu Haipeng Liu Dingchang Zheng Eye Centre The Second Affiliated Hospital of the School of MedicineZhejiang UniversityHangzhouChina Research Centre for Intelligent Healthcare University of CoventryCoventryUK

Purpose:To evaluate the safety and effectiveness of photobiomodulation(PBM)in the treatment of diabetic macular edema(DME).Methods:It was a single-center,self-controlled prospective *** clinical records of 12 diabetic retinopathy patients(5 males and 7 females,20 eyes in total)who were treated with PBM for DME at the Second Affiliated Hospital,Zhejiang University School of Medicine,were *** mean age was 56(26–68)*** the participants received PBM treatment during darkness at night in no less than 5 days per week and no less than 8 h per *** the baseline check and follow-up checks(1,2,6,10,and 12 months after the start of treatment),the best-corrected visual acuity,the thickness of the retina in the macula,and the changes of the fundus lesions were *** signed rank test was used to compare the results before and after treatment.P0.05)from the baseline result,except that in the 6th month(P?0.049).Obvious improvement could be observed in retinal fundus fluorescein angiography ***:PBM is a safe and effective treatment of DME,which deserves further investigation.

关键词： Dark adaptation Diabetic macular edema Diabetic retinopathy Photobiomodulation Rod cells

Transcriptome profile and its partly verification of human hepatocellular carcinoma cells exposed to Yuzhizi(Fructus Akebiae Quinatae) seed extract

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Journal of Traditional Chinese Medicine 2022年第6期42卷 922-931页

作者： FAN Xiaoyan LI Mingzhe CHEN Long LIANG Chao LU Wenli PAN Zhiqiang JIA Dongwei PENG peike FANG Zhaoqin LIU Xiaomei Department of Experimental Traditional Chinese Medicine School of Basic Medical ScienceShanghai University of Traditional Chinese MedicineShanghai 201203China Experiment Center of Science and Technology Shanghai University of Traditional Chinese MedicineShanghai 201203China

OBJECTIVE: To investigate the efficacy of Yuzhizi(Fructus Akebiae Quinatae) seed extract(FAQSE) on inhibiting the proliferation of hepatocellular carcinoma(HCC) cells in vitro and to explore the anti-HCC action mechanism of FAQSE. METHODS: Human HCC Hep G2 and Huh7 cells were used to investigate the anti-HCC effect of FAQSE in vitro. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide(MTT) method was used to measure cell viability. Affymetrix microarray was adopted to detect the expression of transcriptome. The differentially expressed genes(DEGs) of each cell line were identified. For coDEGs of both cell lines, the gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway were enriched using the Database for Annotation, Visualization and Integrated Discovery(DAVID), and the network analysis of protein-protein interaction(PPI) was mapped using the Retrieval of Interacting Genes/Proteins(STRING) and Cytoscape software. Some important genes in the PPI network of coDEGs were selected to verify by quantitative real-time reverse transcription-polymerase chain reaction, Western blot and enzyme-linked immunosorbent assay. RESULTS: FAQSE decreased the viability of Hep G2 and Huh7 cells. There were 211 co-upregulated and 86 codownregualted genes in both cell lines after FAQSE treatment. The enriched GO terms of co-upregulated DEGs were primarily involved cell-cell adhesion, viral process, transcription initiation from RNA polymerase II promoter, positive regulation of transcription from RNA polymerase II promoter and actin cytoskeleton organization. The GO terms of co-downregulated DEGs were mainly enriched in the processes of SRP-dependent cotranslational protein targeting to membrane, viral transcription, nuclear-transcribed m RNA catabolic process, nonsense-mediated decay, translational initiation and r RNA processing. Main KEGG pathways of co-upregulated DEGs were endocytosis, glutathione metabolism, protein processing in endoplasmic reticul

关键词： carcinoma hepatocellular transcriptome alpha-fetoprotein Yuzhizi(Fructus Akebiae Quinatae)seed extract

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Evaluation and application of a Chinese version symptom questionnaire for visual dysfunctions(CSQVD)in school-age children

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Advances in Ophthalmology Practice and Research 2024年第3期4卷 134-141页

作者： Fuyao Chen Lixia Lou Xiaoning Yu peike Hu Weiyi Pan Xuan Zhang Xiajing Tang Eye Center The Second Affiliated Hospital of Zhejiang University School of MedicineZhejiang Provincial Key Laboratory of OphthalmologyZhejiang Provincial Clinical Research Center for Eye DiseasesZhejiang Provincial Engineering Institute on Eye DiseasesHangzhouChina

Objective:To develop and evaluate a Chinese version of the Symptom Questionnaire for Visual Dysfunctions(CSQVD)to quantify visual dysfunction symptoms in school-age children with various eye diseases,and to explore the relationship between ophthalmological disorders and visual dysfunction ***:Following standard scale adaptation procedures,the Symptom Questionnaire for Visual Dysfunctions(SQVD)was translated into Chinese(CSQVD).We employed random sampling to survey 198 outpatients aged 7–18 to assess the psychometric properties of the *** the reliable and validated questionnaire,we evaluated the determinants of visual dysfunction symptoms among 406 school-age patients at an eye *** CSQVD scores were correlated with demographic and clinical variables,including gender,age,eye position,refractive power,and best-corrected visual *** analysis identified potential risk factors,followed by binary logistic regression and multiple linear regression analysis on factors with a P-value<***:The CSQVD scale's critical ratio(CR)values ranged from 6.028 to *** Cronbach's Alpha coeffi-cient was 0.779,and Spearman-Brown split-half reliability was also *** I-CVI varied from 0.83 to 1.000,the S-CVI/Ave was 0.857,and the KMO value was *** regression analysis indicated that high myopia(OR=5.744,95%CI[1.632,20.218],P=0.006)and amblyopia(OR=9.302,95%CI[1.878,46.058],P=0.006)were significant predictors of CSQVD *** linear regression analysis showed that BCVA of amblyopic eyes(B=-5.052,95%CI[7.779,2.325],P=0.000)and SE power(B=-0.234,95%CI[-0.375,0.205],P=0.001)significantly affected the CSQVD scale ***:The Chinese version of the SQVD scale(CSQVD)demonstrates good feasibility,discriminatory power,validity,and reliability in assessing Chinese school-aged ***,those who have severe myopia and amblyopia reported more visual dysfunction symptoms.

关键词： Visual function School-age children Visual quality Scale Reliability Validity

String Stability of a Third-order Vehicle Model with Inertial Delay Parameters Under Bilateral Control

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String Stability of a Third-order Vehicle Model with Inertia...

Design of Fractional Order Controller for Microgrid Based on Model Analysis

第40届中国控制会议

作者： Daxing Zhong Min Zheng peike Li College of Mechatronic Engineering and Automation Shanghai University Shanghai Key Laboratory of Power Station Automation Technology

In this paper, the string stability of bilateral-control systems consisting of third-order models with inertial time-delay parameters is studied. Unlike the double integral model which is always stable under bilateral control conditions, the stability of the third order model can only be guaranteed under constraints. In this paper, the closedloop stability of large scale platoon is solved by matrix factorization and converted into the stability analysis of the order of the child nodes, and the closed-loop stability conditions are given. On this basis, the string stability of the platoon of vehicles was further studied. By transforming the system matrix into Toeplitz form, the general solution formula of the system equation is obtained. Combined with contradiction proof and inequality analysis, it is proved that the platoon is always string stable under the condition of closed-loop stability. Finally, the conclusion is verified by computer simulation.

关键词： Third-order vehicle model bilateral control string stability distributed linear controller

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Design of Fractional Order Controller for Microgrid Based on...

猪场沼液UF-MBR+RO处理工艺浓缩液回流的盐积累模型

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作者： peike Li Min Zheng Daxing Zhong Yang Zheng Guangfeng Zhang College of Mechatronic Engineering and Automation Shanghai University Shanghai Key Laboratory of Power Station Automation Technology

With the development of power electronics technology, people pay more attention to power quality. In order to stabilize the current and voltage of AC side in microgrid, a control strategy based on PWM technology is proposed. D-q transformation is usually used to solve the problem of three-phase variable *** to the decoupled mathematical model, PWM control strategy is divided into inner loop current control and outer loop voltage control. PI controller is usually used to control the inner loop current and outer loop voltage. However, the dynamic characteristics and robustness of the traditional PI control system has been gradually unable to meet the requirements of complex power grid. In order to improve the dynamic characteristics and robustness of the system, the inner loop current is controlled by fractional order controller based on internal model control, fractional order PI controller is used to control the outer loop voltage. The fractional order controller has strong robustness, but its parameters are difficult to be solved. In this paper, a method of fractional order PID parameter tuning based on model analysis is proposed. Compared with the traditional integer-order PI controller, the fractional-order controller has stronger robustness, can overcome the problem of model accuracy to a certain extent, and has better control performance. Finally, the simulation results show the effectiveness of the proposed method.

关键词： PWM control strategy d-q transformation fractional order PI model analysis

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农业工程学报 2021年第13期37卷 209-215页

作者：蒋小妹李俊王炯科伍佩珂邓良伟王文国农业农村部沼气科学研究所成都610041 成都大学食品与生物工程学院成都610106

反渗透(ReverseOsmosis,RO)膜工艺在沼液深度处理中发挥重要作用,其浓缩液回流引起的盐积累会降低生化阶段的效能。该研究模拟猪场沼液超滤(Ultrafiltration,UF)-膜生物反应器(Membrane Bioreactor,MBR)+RO处理工艺中浓缩液回流,构建盐... 详细信息

反渗透(ReverseOsmosis,RO)膜工艺在沼液深度处理中发挥重要作用,其浓缩液回流引起的盐积累会降低生化阶段的效能。该研究模拟猪场沼液超滤(Ultrafiltration,UF)-膜生物反应器(Membrane Bioreactor,MBR)+RO处理工艺中浓缩液回流,构建盐积累模型预测不同污泥停留时间(Sludge Retention Time,SRT)下UF-MBR中盐积累量,分析污泥吸附作用对盐积累模型准确度的影响。结果表明:构建的盐积累模型可预测盐积累量及达到盐平衡所需的回流次数,Ca^(2+)、Mg^(2+)的实际值与理论值的拟合决定系数R2高于0.95,RMSE小于4.00mg/L,模型对Ca^(2+)、Mg^(2+)积累量预测的准确度高。SRT从60 d降低至30 d,盐度从4.83%降低至2.63%,达到盐平衡所需的时长从249 d降低至179 d,降低SRT可作为一种有效策略来降低MBR中盐积累量及达到盐平衡的时长。SRT控制在30 d以下可使MBR盐度低于1.00%,使MBR生化阶段发挥效能的高效性。此外,污泥的吸附可降低MBR中积累的K+、Na+的含量。但是,Ca^(2+)、Mg^(2+)累积量较高时,污泥吸附作用对模型的影响较低,该研究构建的模型可为猪场沼液UF-MBR+RO处理工艺的应用提供参考。

关键词：模型盐污泥膜浓缩液回流污泥停留时间污泥吸附

模块化多电平变换器分数阶PIλ环流抑制策略

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电力系统保护与控制 2023年第1期51卷 113-121页

作者：郑征李佩柯李绍令王肖帅靳婷婷河南理工大学电气工程与自动化学院河南焦作454000

模块化多电平变换器(modular multilevel converter,MMC)内部环流增加系统功率损耗,加剧桥臂电流畸变。针对传统PI环流控制器存在鲁棒性较差、控制精度有限等问题,将分数阶PI^(λ)控制器应用于MMC环流抑制。首先,对MMC工作特性进行分析... 详细信息

模块化多电平变换器(modular multilevel converter,MMC)内部环流增加系统功率损耗,加剧桥臂电流畸变。针对传统PI环流控制器存在鲁棒性较差、控制精度有限等问题,将分数阶PI^(λ)控制器应用于MMC环流抑制。首先,对MMC工作特性进行分析,阐述环流产生机理。其次,研究分数阶PI^(λ)控制结构,并设计MMC分数阶PI^(λ)环流抑制策略。然后,采用经典频域法优化分数阶PI^(λ)控制器参数,并对分别采用传统PI和分数阶PI^(λ)控制器的系统环流控制性能进行对比分析。最后,在相同条件下,通过对采用上述两种环流抑制策略的MMC系统,分别在电源扰动、直流侧电压阶跃和三相不平衡工况下进行仿真研究。结果表明,分数阶PI^(λ)环流抑制器的抗电源扰动性和环流抑制能力均优于传统PI环流抑制器,更适用于MMC此类的非线性系统的控制。

关键词：模块化多电平变换器环流抑制分数阶FO-PIλ

校园文化培育大学生社会责任感的“四维模式”

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现代教育管理 2014年第2期 104-107页

作者：乔佩科辽宁地质工程职业学院辽宁丹东118008

校园文化有助于大学生社会责任感的培育,校园文化所包含的精神文化、制度文化、显性文化、隐性文化成为培育大学生社会责任感的"四维模式",其中,校风、教风、学风等精神文化表现载体成为培养大学生社会责任感的重要因子,制度文化通过对... 详细信息

校园文化有助于大学生社会责任感的培育,校园文化所包含的精神文化、制度文化、显性文化、隐性文化成为培育大学生社会责任感的"四维模式",其中,校风、教风、学风等精神文化表现载体成为培养大学生社会责任感的重要因子,制度文化通过对学风、道德、意识等方面发挥作用来为大学生社会责任感培养提供必要的保证,物质、景观等硬实力方面的显性文化与社团活动、"书香校园"工程等软实力方面的隐性文化也用各自的模式影响着大学生社会责任感的培育。

关键词：校园文化社会责任感四维模式

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油藏微生物的代谢特征和生态结构调控

微生物学报 2011年第6期51卷 711-717页

作者：高配科马挺刘如林南开大学生命科学学院分子微生物与技术教育部重点实验室天津300071

油藏环境中孕育着多种多样的微生物,这些微生物代谢类型多、变异性大,在微生物生态系统中占有重要位置。研究油藏中微生物的代谢特征和相互之间的生态关系,有助于提升对微生物提高采收率机理的认识。本文对油藏环境中常见微生物类群的... 详细信息

油藏环境中孕育着多种多样的微生物,这些微生物代谢类型多、变异性大,在微生物生态系统中占有重要位置。研究油藏中微生物的代谢特征和相互之间的生态关系,有助于提升对微生物提高采收率机理的认识。本文对油藏环境中常见微生物类群的代谢特征与功能、生态结构与调控等进行了简要综述。

关键词：油藏微生物代谢特征生态调控提高采收率