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Control Theory and Technology 2019年第4期17卷 309-324页

作者： Qiuyi GUO Zhiguo ZHAO peihong shen Peidong ZHOU National Engineering Laboratory for Clean Energy Automotives and Powertrain Systems School of Automotive StudiesTongji UniversityShanghai 201804China

Energy optimization management can make fuel cell truck(FCT)power system more efficient,so as to improve vehicle fuel *** the structure of power source system and the torque distribution strategy are determined,the essence is to find the reasonable distribution of electric power between the fuel cell and other energy *** paper simulates the assistance of the intelligent transport system(ITS)and carries out the eco-velocity planning using the traffic signal *** this basis,in order to further improve the energy efficiency of FCT,a model predictive control(MPC)-based energy source optimization management strategy is innovatively developed,which uses Dijkstra algorithm to achieve the minimization of equivalent hydrogen *** the scenarios of signalized intersections,based on the planned eco-velocity,the off-line simulation results show that the proposed MPC-based energy source management strategy(ESMS)can reduce hydrogen consumption of fuel cell up to 7%compared with the existing rule-based ***,the Hardware-in-the-Loop(HiL)simulation test is carried out to verify the effectiveness and real-time performance of the proposed MPC-based energy source optimization management strategy for the FCT based on eco-velocity planning with the assistance of traffic light information.

关键词： Fuel cell truck hybrid energy source management strategy model predictive control traffic light

Changes of composition and antibiotic resistance of fecal coliform bacteria in municipal wastewater treatment plant

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Journal of Environmental Sciences 2024年第12期146卷 241-250页

作者： Luoyao Wen Yunwei Cui Luodong Huang Chunzhong Wei Gangan Wang Junya Zhang Yanbo Jiang Yuansong Wei peihong shen State Key Joint Laboratory of Environmental Simulation and Pollution Control Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China College of Life Science and Technology Guangxi UniversityNanning 530005China Nanning Engineering&Technology Research Center for Water Safety Guangxi Beitou Environmental Protection&Water Group Co.LTD.Nanning 530025China Department of Isotope Biogeochemistry Helmholtz Centre for Environmental Research–UFZLeipzig 04318Germany University of Chinese Academy of Sciences Beijing 100049China International Graduate School at shenzhen Tsinghua UniversityShenzhen 518055China

The dynamics of the composition and antibiotic resistance of the fecal coliform bacteria(FCB)in a typical wastewater treatment plant(WWTP)were investigated concerning the seasonal *** showed that WWTP could remove the FCB concentration by 3∼5 logs within the effluent of 10^(4)∼10^(5)CFU/L,but the antibiotic resistant rate of FCB species increased significantly after *** dominant FCB changed from Escherichia coli in the influent(∼73.0%)to Klebsiella pneumoniae in the effluent(∼53.3%)after WWTP,where the Escherichia coli was removed the most,while Klebsiella pneumoniae was the most *** secondary tank removed the most of FCB(by 3∼4 logs)compared to other processes,but increased all the concerned antibiotic resistant *** potential super bugs of FCB community showing resistance to all the target antibiotics were selected in the biological treatment unit of *** FCB showed the highest multiple antibiotic resistance(92.9%)in total which even increased to 100%in the *** has the highest antibiotic resistant rate in FCB,with a multiple antibiotic resistance rate of 98.4%.These indicated that the Klebsiella pneumoniae not just Escherichia coli should be specially emphasized after WWTP concerning the health risk associated with FCB community.

关键词： Fecal coliform bacteria Antibiotic resistance Wastewater treatment plant Antibiotic resistant bacteria Multiple antibiotic resistance

Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysis

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Food Science and Human Wellness 2024年第4期13卷 2048-2059页

作者： Xueyan Mo Mengcheng Zhou Yanmei Li Lili Yu Huashang Bai peihong shen Xing Zhou Haojun Zhu Huijie Sun Ru Bu Chengjian Jiang State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi Research Center for Microbial and Enzyme Engineering TechnologyCollege of Life Science and TechnologyGuangxi UniversityNanning 530004China National Engineering Research Center for Non-Food Biorefinery State Key Laboratory of Non-Food Biomass and Enzyme TechnologyGuangxi Research Center for Biological Science and TechnologyGuangxi Academy of SciencesNanning 530007China Guangxi Key Laboratory for Green Processing of Sugar Resources College of Biological and Chemical EngineeringGuangxi University of Science and TechnologyLiuzhou 545006China

The application of microorganisms as probiotics is limited due to lack of safety ***,a novel multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 with aroma-producing properties was identified from marine mangrove *** safety and probiotic properties were assessed in accordance with phenotype and whole-genome sequencing *** showed that the genes and phenotypic expression of related virulence,antibiotic resistance and retroelement were rarely *** morphogenesis genes(SIT4,HOG1,SPA2,ERK1,ICL1,CST20,HSP104,TPS1,and RHO1)and phospholipase secretion gene(VPS4)were *** hyphae and phospholipase were *** one retroelement(Tad1-65_BG)was *** biogenic amines(BAs)encoding genes were absent,except for spermidine synthase(JA9_002594),spermine synthase(JA9_004690),and tyrosine decarboxylase(inx).The production of single BAs and total BAs was far below the food-defined ***6 had no resistance to common antifungal *** enzymes,such as gelatinase,DNase,hemolytic,lecithinase,and thrombin were *** toxicity test with mice demonstrated that GXDK6 is ***6 has a good reproduction ability in the simulation gastrointestinal ***6 also has a strong antioxidant ability,β-glucosidase,and inulinase *** sum up,GXDK6 is considered as a safe probiotic for human consumption and food fermentation.

关键词： Meyerozyma guilliermondii Safety assessment Probiotics Marine mangrove microorganisms Whole-genome sequencing analysis

Regulatory mechanisms of dopamine metabolism in a marine Meyerozyma guilliermondii GXDK6 under NaCl stress as revealed by integrative multi-omics analysis

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Synthetic and Systems Biotechnology 2024年第1期9卷 115-126页

作者： Huijie Sun Huashan Bai Yonghong Hu sheng He Ruihang Wei Duotao Meng Qiong Jiang Hongping Pan peihong shen Qian Ou Chengjian Jiang State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Guangxi Research Center for Microbial and Enzyme Engineering TechnologyCollege of Life Science and TechnologyGuangxi UniversityNanning530004China Guangxi Key Laboratory for Green Processing of Sugar Resources College of Biological and Chemical EngineeringGuangxi University of Science and TechnologyLiuzhou545006China Guangxi Key Laboratory of Birth Defects Research and Prevention Guangxi Key Laboratory of Reproductive Health and Birth Defect PreventionGuangxi Zhuang Autonomous Region Women and Children Health Care HospitalNanning530033China College of Food Science and Light Industry Nanjing Tech UniversityNo.30South Puzhu RoadNanjing211816China

Dopamine can be used to treat depression,myocardial infarction,and other ***,few reports are available on the de novo microbial synthesis of dopamine from low-cost *** this study,integrated omics technology was used to explore the dopamine metabolism of a novel marine multi-stress-tolerant aromatic yeast Meyerozyma guilliermondii ***6 was found to have the ability to biosynthesize dopamine when using glucose as the substrate.14 key genes for the biosynthesis of dopamine were identified by whole genome-wide *** and proteomic data showed that the expression levels of gene AAT2 encoding aspartate aminotransferase(regulating dopamine anabolism)were upregulated,while gene AO-I encoding copper amine oxidase(involved in dopamine catabolism)were downregulated under 10%NaCl stress compared with non-NaCl stress,thereby contributing to biosynthesis of ***,the amount of dopamine under 10%NaCl stress was 2.51-fold higher than that of zero NaCl,which was consistent with the multi-omics ***-time fluorescence quantitative PCR(RT-qPCR)and high-performance liquid chromatography(HPLC)results confirmed the metabolic model of ***,by overexpressing AAT2,AST enzyme activity was increased by 24.89%,the expression of genes related to dopamine metabolism was enhanced,and dopamine production was increased by 56.36%in recombinant *** conclusion,Meyerozyma guilliermondii GXDK6 could utilize low-cost carbon source to synthesize dopamine,and NaCl stress promoted the biosynthesis of dopamine.

关键词： Dopamine biosynthesis Multi-omics analysis NaCl stress Meyerozyma guilliermondii

Research and Prospect Analysis of Microorganisms in Selenium-Rich Soil

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Plant Diseases and Pests 2020年第4期11卷 36-38,46页

作者： Huang Chanchan Liu Yongxian Pan Liping Liao Qing shen peihong Institute of Agricultural Resources and Environment Guangxi Academy of Agricultural Sciences/Guangxi Engineering Technology Research Center for Selenium-Rich Agricultural ProductsNanning 530007China Guangxi University Nanning 530005China Guangxi Selenium-Rich Agricultural Research Center Nanning 530007China Guangxi Plant Nutrition Engineering Technology Research Center Nanning 530007China

The importance and application prospect of microorganisms in selenium-rich soil are discussed from five aspects:the distribution of selenium-rich soil,research status at home and abroad,main groups of microorganisms i... 详细信息

关键词： Selenium Soil Microorganism

By Protein and Gene Levels,Detection of P53,Ki67 Expression and TNM Staging Correlation in Triple Negative Breast Cancer

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By Protein and Gene Levels,Detection of P53,Ki67 Expression ...

To investigate the expression of tumor markers C-erbB-2 and CK5/6 in esoph-ageal squamous cell carcinoma and correlation analysis of TNM staging

2019年中国肿瘤标志物学术大会暨第十三届肿瘤标志物青年科学家论坛

作者： Zhao,Lili Li,Qingying Zhang,Li shen,peihong Cancer Hospital Affiliated to Zhengzhou University The first affiliated hospital of henan university

Objective:This article purpose was exploring the clinical significance of the correlation study between P53 and Ki67 expression and pathological TNM staging in triple negative breast cancer (TNBCs) and non-TNBCs *** to combining with clinical follow-up data,analysis the relationship between TNBCs and prognosis of TNBCs patients and non-tnbcs.

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To investigate the expression of tumor markers C-erbB-2 and ...

Age distribution of molecular subtypes of invasive breast cancer and correlati-on analysis of various clinical parameters

2019年中国肿瘤标志物学术大会暨第十三届肿瘤标志物青年科学家论坛

作者： yang,chenbo Zhang,Li Zhao,Lili shen,peihong Cancer Hospital Affiliated to Zhengzhou University The first affiliated hospital of henan university

Objective:To study the clinical significance of C-erbB-2,CK5/6 expression in esophageal squamous cell carcinoma and its correlation with TNM staging,and to explore potential targets for clinical prevention or treatmen...

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Age distribution of molecular subtypes of invasive breast ca...

基于ANFIS的工况跟踪用纵向驾驶员模型开发

2019年中国肿瘤标志物学术大会暨第十三届肿瘤标志物青年科学家论坛

作者： Zhao,Lili Li,Qingying Zhang,Li Yang,Chenbo shen,peihong Cancer Hospital Affiliated to Zhengzhou University The first affiliated hospital of henan university

Objective:To explore the correlation between age distribution and clinical parameters of four different molecular subtypes of breast ***:Retrospective analysis selected 516 patients with breast cancer who were clinically diagnosed from January 2017 to December 2018 in the Provincial Cancer Hospital of Zhengzhou University.

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汽车工程 2019年第7期41卷 815-822,791页

作者：沈沛鸿赵治国郭秋伊同济大学汽车学院

针对基于PI控制纵向驾驶员模型的工况跟踪效果不佳等问题,采集了某PHEV试验样车的纵向驾驶行为数据,采用ANFIS建立了二输入参数的纵向驾驶员模型和考虑未来预期车速影响的预瞄纵向驾驶员模型。仿真结果表明,基于ANFIS的预瞄纵向驾驶员... 详细信息

针对基于PI控制纵向驾驶员模型的工况跟踪效果不佳等问题,采集了某PHEV试验样车的纵向驾驶行为数据,采用ANFIS建立了二输入参数的纵向驾驶员模型和考虑未来预期车速影响的预瞄纵向驾驶员模型。仿真结果表明,基于ANFIS的预瞄纵向驾驶员模型具有最好的工况跟踪效果,均方根误差为0.993 0 km/h,且其决策出的加速踏板行程、挡位和需求转矩与实车试验结果最为接近。

关键词：纵向驾驶员模型自适应网络模糊推理系统预瞄

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白光LED照明技术进展及产业和市场现状

灯与照明 2006年第2期30卷 46-52页

作者：沈培宏华东电子集团公司南京210028

半导体照明是本世纪最具发展前景的高技术领域之一,白光LED将成为21世纪的新一代光源———第四代电光源,可以替代白炽灯、荧光灯和高压气体放电灯等传统光源,白光LED孕育着巨大的商机。该文阐述LED的基本原理、特点和发展及应用前景,... 详细信息

半导体照明是本世纪最具发展前景的高技术领域之一,白光LED将成为21世纪的新一代光源———第四代电光源,可以替代白炽灯、荧光灯和高压气体放电灯等传统光源,白光LED孕育着巨大的商机。该文阐述LED的基本原理、特点和发展及应用前景,并介绍白光LED的市场和产业现状。

关键词：白光LED 半导体照明发光二极管固体光源