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Trimethylamine N-oxide aggravates vascular permeability and endothelial cell dysfunction under diabetic condition:in vitro and in vivo study

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International Journal of Ophthalmology(English edition) 2024年第1期17卷 25-33页

作者： Jia-Yi Jiang Wei-Ming Liu Qiu-Ping Zhang Hang Ren Qing-Ying Yao Gao-Qin Liu pei-rong lu Department of Ophthalmology the First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina Suzhou Center for Disease Prevention and Control Suzhou 215004Jiangsu ProvinceChina

AIM:To provide the direct evidence for the crucial role of trimethylamine N-oxide(TMAO)in vascular permeability and endothelial cell dysfunction under diabetic ***:The role of TMAO on the in vitro biological effect of human retinal microvascular endothelial cells(HRMEC)under high glucose conditions was tested by a cell counting kit,wound healing,a transwell and a tube formation *** inflammation-related gene expression affected by TMAO was tested by real-time polymerase chain reaction(RT-PCR).The expression of the cell junction was measured by Western blotting(WB)and immunofluorescence *** addition,two groups of rat models,diabetic and non-diabetic,were fed with normal or 0.1%TMAO for 16wk,and their plasma levels of TMAO,vascular endothelial growth factor(VEGF),interleukin(IL)-6 and tumor necrosis factor(TNF)-αwere *** vascular permeability of rat retinas was measured using FITC-Dextran,and the expression of zonula occludens(ZO)-1 and claudin-5 in rat retinas was detected by WB or immunofluorescence ***:TMAO administration significantly increased the cell proliferation,migration,and tube formation of primary HRMEC either in normal or high-glucose ***-PCR showed elevated inflammation-related gene expression of HRMEC under TMAO stimulation,while WB or immunofluorescence staining indicated decreased cell junction ZO-1 and occludin expression after high-glucose and TMAO *** rats showed higher plasma levels of TMAO as well as retinal vascular leakage,which were even higher in TMAO-feeding diabetic ***,TMAO administration increased the rat plasma levels of VEGF,IL-6 and TNF-αwhile decreasing the retinal expression levels of ZO-1 and ***:TMAO enhances the proliferation,migration,and tube formation of HRMEC,as well as destroys their vascular integrity and tight *** also regulates the expression of VEGF,IL-6,and TNF-α.

关键词： diabetic model trimethylamine N-oxide inflammation endothelial dysfunction rats retinal microvascular endothelial cells

Resolution of myopic macular schisis following cataract phacoemulsification

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International Journal of Ophthalmology(English edition) 2024年第8期17卷 1568-1570页

作者： Yan-Ting Li Bo Jiang Xin-Zhu Chen Hang Ren Yan-Hui Xiao Zi-Yue Song pei-rong lu Department of Ophthalmology the First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina Department of Ophthalmology Suzhou EENT HospitalSuzhou 215000Jiangsu ProvinceChina

Dear Editor,We write to introduce a case of high myopia who had bilateral macular schisis along with *** the following-up period from 2014 to 2021,the severity of macular schisis gradually ***,following cataract surgery,there was a gradual improvement in the macular schisis,ultimately leading to near-complete *** obtained the written informed consent from the patient,and this case study adhered to the principles in the Declaration of Helsinki.

关键词： cataract sch ultimately

Corneal collagen crosslinking in keratoconus and other eye disease

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International Journal of Ophthalmology(English edition) 2015年第2期8卷 407-418页

作者： Adel Alhayek pei-rong lu Department of Ophthalmology the First Affiliated Hospital of Soochow University

Keratoconus is a condition characterized by biomechanical instability of the cornea, presenting in a progressive, asymmetric and bilateral way. Corneal collagen crosslinking(CXL) with riboflavin and Ultraviolet-A(UVA) is a new technique of corneal tissue strengthening that combines the use of riboflavin as a photo sensitizer and UVA irradiation. Studies showed that CXL was effective in halting the progression of keratoconus over a period of up to four years. The published studies also revealed a reduction of max K readings by more than 2 D, while the postoperative spherical equivalent(SEQ) was reduced by an average of more than 1 D and refractive cylinder decreased by about1 D. The major indication for the use of CXL is to inhibit the progression of corneal ecstasies, such as keratoconus and pellucid marginal degeneration. CXL may also be effective in the treatment and prophylaxis of iatrogenic keratectasia, resulting from excessively aggressive photo ablation. This treatment has been used to treat infectious corneal ulcers with apparent favorable results. Most recent studies demonstrate the beneficial impact of CXL for iatrogenic ecstasies, pellucid marginal degeneration, infectious keratitis, bullous keratopathy and ulcerative keratitis. Several long-term and short-term complications of CXL have been studied and documented. The possibility of a secondary infection after the procedure exists because the patient is subject to epithelial debridement and the application of a soft contact lens. Formation of temporary corneal haze,permanent scars, endothelial damage, treatment failure,sterile infiltrates, bullous keratopathy and herpes reactivation are the other reported complications of this procedure.

关键词： keratoconus collagen corneal cross-linking ultraviolet radiation and riboflavin

Inhibitory effect of CCR3 signal on alkali-induced corneal neovascularization

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International Journal of Ophthalmology(English edition) 2012年第3期5卷 251-257页

作者： Wen-Juan Zhou, pei-rong lu Department of Ophthalmology the First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China Jiangsu Institute of Clinic Immunology the First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China

AIM: To investigate the effect of CC chemokine receptor 3 (CCR3) signal on corneal neovascularization (CRNV) induced by alkali burn and to explore its mechanism. METHODS: Specific pathogen-free male BALB/C mice (aged 6-8 weeks) were randomly divided into CCR3-antagonist treated group (experimental group) and control group. CRNV was induced by alkali burn in mice. The time kinetic CCR3 expression in injured corneas was examined by reverse transcription polymerase chain reaction (RT-PCR). CCR3-antagonist (SB-328437 at different concentration of 125 mu g/mL, 250 mu g/mL, and 500 mu g/mL) was locally administrated after alkali injury. The formation of CRNV was assessed by CD31 corneal whole mount staining at two weeks after injury. Monocyte chemotactic protein 1 (MCP-1), monocyte chemotactic protein 3 (MCP-3) expressions in the early phase after injury were quantified and compared by RT-PCR. Macrophage intracorneal accumulation in the early phase after injury was evaluated and compared by immunohistochemistry. RESULTS: Alkali injury induced the time kinetic intracorneal CCR3 expression. 500 mu g/mL of CCR3-antagonist treatment in the early phase but not the late phase resulted in significant impaired CRNV as compared to control group (P luSION: CCR3 signal is involved in alkali-induced CRNV. CCR3-antagonist can inhibit alkali-induced CRNV by reducing the intracorneal MCP-1 and MCP-3 mRNA expression and the intracorneal macrophage infiltration.

关键词： corneal neovascularization CCR3 monocyte chemotactic protein 1 monocyte chemotactic protein 3 macrophage

Subcutaneous pedicled propeller flap technique for microscopic reconstruction of eyelid defects

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International Journal of Ophthalmology(English edition) 2023年第7期16卷 1047-1052页

作者： Lei Zhong Qian-Yi lu pei-rong lu The First Affiliated Hospital of Soochow University Suzhou 215006Jiangsu ProvinceChina

AIM:To describe the subcutaneous pedicled propeller flap technique for the microscopic reconstruction of eyelid defects and evaluate its ***:The clinical data of 23 patients(23 eyes)who underwent microscopic reconstruction of eyelid defects with the subcutaneous pedicled propeller flap technique were retrospectively *** patients underwent eyelid tumor resection and one-stage microscopic reconstruction with the subcutaneous pedicled propeller flap for anterioror posterior-layer eyelid *** survival rate of the propeller flap,eyelid function and appearance,tumor recurrence rate,and patient satisfaction were evaluated after the ***:The patients consisted of 12 men and 11 women,aged 31–82y(mean,58.9y).The longest followup time was 5y,and the shortest was *** the propeller flaps survived *** was no significant difference in color and luster between the flap and adjacent tissues,and there was no dog ear *** obvious scarring was *** were no obvious abnormalities of eyelid morphology or function,and no adverse complications such as exposure keratitis,entropion,ectropion,ptosis,and eyelid *** tumor recurrence was found at the time of the last *** patients were satisfied with the surgical ***:The subcutaneous pedicled propeller flap technique for the microscopic reconstruction of eyelid defects has satisfactor y outcomes in terms of eyelid function and esthetics,and merits clinical application.

关键词： propeller flap eyelid defect microscopic reconstruction

Retinal ganglion cell-inner plexiform and nerve fiber layers in neuromyelitis optica

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International Journal of Ophthalmology(English edition) 2018年第1期11卷 89-93页

作者： Sai-Jing Hu pei-rong lu Department of Ophthalmology the First Affiliated Hospital of Soochow University Department of Ophthalmology the First Affiliated Hospital of Wenzhou Medical University

AIM： To determine the thickness of the retinal ganglion cell-inner plexiform layer（GCIPL） and the retinal nerve fiber layer（RNFL） in patients with neuromyelitis optica（NMO）.METHODS： We conducted a cross-sectional study that included 30 NMO patients with a total of 60 eyes. Based on the presence or absence of optic neuritis（ON）, subjects were divided into either the NMO-ON group（30 eyes） or the NMO-ON contra group（10 eyes）. A detailed ophthalmologic examination was performed for each group; subsequently, the GCIPL and the RNFL were measured using highdefinition optical coherence tomography（OCT）. RESULTS： In the NMO-ON group, the mean GCIPL thickness was 69.28±21.12 μm, the minimum GCIPL thickness was 66.02±10.02 μm, and the RNFL thickness were 109.33±11.23, 110.47±3.10, 64.92±12.71 and 71.21±50.22 μm in the superior, inferior, temporal and nasal quadrants, respectively. In the NMO-ON contra group, the mean GCIPL thickness was 85.12±17.09 μm, the minimum GCIPL thickness was 25.39±25.1 μm, and the RNFL thicknesses were 148.33±23.22, 126.36±23.45, 82.21±22.30 and 83.36±31.28 μm in the superior, inferior, temporal and nasal quadrants, respectively. In the control group, the mean GCIPL thickness was 86.98±22.37 μm, the minimum GCIPL thickness was 85.28±10.75 μm, and the RNFL thicknesses were 150.22±22.73, 154.79±60.23, 82.33±7.01 and 85.62±13.81 μm in the superior, inferior, temporal and nasal quadrants, respectively. The GCIPL and RNFL were thinner in the NMO-ON contra group than in the control group（P〈0.05）; additionally, the RNFL was thinner in the inferior quadrant in the NMO-ON group than in the control group（P〈0.05）. Significant correlations were observed between the GCIPL and RNFL thickness measurements as well as between thickness measurements and the two visual field parameters of mean deviation（MD） and corrected pattern standard deviation（PSD） in the NMO-ON group（P〈0.05）. CONCluSION： The thickness of the GCIPL and RNFL, as measured using OCT, may indicate optic

关键词： neuromyelitis optica retinal nerve fiber layer thickness ganglion cell-inner plexiform layer optical coherence tomography

High glucose: activating autophagy and affecting the biological behavior of human lens epithelial cells

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International Journal of Ophthalmology(English edition) 2019年第7期12卷 1061-1066页

作者： Dan Li Gao-Qin Liu pei-rong lu Department of Ophthalmology the First Affiliated Hospital of Soochow University

AIM: To clarify the effect of autophagy on human lens epithelial cells (HLECs) under high glucose ***: HLECs were cultured with different concentrations of glucose and 3-methyladenine (3-MA);the expression of autophagy-related protein LC3B was detected by Western blotting and immunofluorescence histochemistry. The migration of HLECs was quantified by scratch wound assay and the expression of transforming growth factor-β(TGF-β) was measured by real-time polymerase chain ***: Compared with 5 mmol/L normal glucose treatment, 40 mmol/L glucose treatment can significantly increase the gen eration of autophagosome in HLECs, which could be inhibited by 0.375 mmol/L 3-MA treatment. The migration of HLECs and the expression of TGF-β in HLECs induced by high glucose were significantly suppressed by 0.375 mmol/L 3-MA ***: Autophagy promotes HLECs cell migration and increases the expression of TGF-β after exposed to high glucose, which may relate to the development of diabetic cataract.

关键词： high glucose autophagy lens epithelial cells

Potential involvement of nitric oxide synthase but not inducible nitric oxide synthase in the development of experimental corneal neovascularization

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International Journal of Ophthalmology(English edition) 2011年第4期4卷 343-348页

作者： Yuan Chen Gao-Qin Liu and pei-rong lu Department of Ophthalmology the First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China

AIM: To investigate the effect of nitric oxide and its synthetase on experimental corneal neovascularization (CRNV). METHODS: CRNV was induced by alkali injury in mice, nitric oxide synthetase (NOS) was inhibited by NG-nitro-L-arginine (L-NAME) and inducible nitric oxide synthetase (iNOS) was inhibited by aminoguanidine hemisulfate salt (AG). The inhibitory effect was detected at day 2 and 4 after corneal alkali injury by reverse transcription polymerase chain reaction (RT-PCR). CRNV was compared between the control and the treated mice by microscopic observation and corneal whole mount CD31 immunostaining. RESULTS: The inhibition of L-NAME to NOS and AG to iNOS after corneal injury was confirmed by RT-PCR (P 0.05). CONCluSION: Our findings suggest that NOS but not iNOS plays a critical role in alkali injury induced CRNV.

关键词： corneal neovascularization nitric oxide synthase inducible nitric oxide synthase

Proteomic analysis of anti-angiogenic effects by conbercept in the mice with oxygen induced retinopathy

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International Journal of Ophthalmology(English edition) 2020年第12期13卷 1844-1853页

作者： Ji Jin Lei Chen Gao-Qin Liu pei-rong lu Department of Ophthalmology the First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina Department of Ophthalmology the Second Affiliated Hospital of Soochow UniversitySuzhou 215004Jiangsu ProvinceChina Department of Ophthalmology Children's Hospital of Soochow UniversitySuzhou 215025Jiangsu ProvinceChina

AIM:To analyze the retinal proteomes with and without conbercept treatments in mice with oxygen-induced retinopathy(OIR)and identify proteins involved in the molecular mechanisms mediated by ***:OIR was induced in fifty-six C57 BL/6 J mouse pups and randomly divided into four *** 1:Normal17(n=7),mice without OIR and treated with normal *** 2:OIR12/EXP1(n=14),mice received 75%oxygen from postnatal day(P)7 to *** 3:OIR17/Control(n=14),mice received 75%oxygen from P7 to P12 and then normal air to *** 4:Lang17/EXP2(n=21),mice received 75%oxygen from P7 to P12 with intravitreal injection of 1μL conbercept at the concentration of 10 mg/m L at P12,and then normal air from P12 to *** ChromatographyMass Spectrometry(LC-MS)/MS data were reviewed to find proteins that were up-regulated after the conbercept *** ontology(GO)analysis was performed of conbercept-mediated changes in proteins involved in single-organism processes,biological regulation,cellular processes,immune responses,metabolic processes,locomotion and multiple-organism ***:Conbercept induced a reversal of hypoxiainducible factor 1 signaling pathway as revealed by the Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis and also induced down-regulation of proteins involved in blood coagulation and fibrin clot formation as demonstrated by the Database for Annotation,Visualization and Integrated Discovery(DAVID)and the stimulation of interferon genes *** appear to be risk factors of retinal *** conbercept-specific fibrosis risk factors were also identified and may serve as therapeutic targets for ***:Our studies reveal that many novel proteins are differentially regulated by *** new insights may warrant a valuable resource for conbercept treatment.

关键词： anti-vascular endothelial growth factor conbercept retinopathy fibrosis proteomic analysis