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Molecular quantification of herbs(Herb-Q): a pyrosequencing-based approach and its application in Pinellia ternata

Chinese Journal of Natural Medicines 2024年第07期 663-672页

作者： pei Yifei LiU Ziyi YU Dade ZHANG Xiangyu SUN wei CHEN Xiaofang FENG Xue Li Xiwen institute of Chinese Materia Medica China Academy of Chinese Medical Sciences Bijie institute of Traditional Chinese Medicine The Key Laboratory for Health industry of Bijie Bijie Medical College

Variations in herb dosage due to species adulteration and dosing inaccuracies can substantially affect clinical safety and efficacy. Accurate species quantification remains challenging, as current methods often yield inconsistent results. This study introduces a novel pyrosequencing-based technique, termed herb molecular quantification（Herb-Q）, designed to precisely quantify herbal products. We evaluated its effectiveness using Pinellia ternata and five of its adulterants. initially, we assessed commonly used DNA barcodes with sequences from a public database, identifying two candidate regions, Maturase K（matK） and internal transcribed spacer 2（iTS2）, for screening specific single nucleotide polymorphism（SNP） loci, allowing for species-specific identification. These loci were validated by amplifying and sequencing genomic material from collected samples. Our validation studies showed that Herb-Q demonstrated excellent linearity, accuracy, repeatability, and detection limits. We established quantitative standard curves with high R2values（> 0.99） to enable precise species quantification, which were combined with external standards to provide clear and accurate visual quantification results. The average bias in quantifying the tuber of P. ternata was 2.38%, confirming that Herb-Q can accurately identify and quantify herbal product constituents. Moreover, the entire quantification process took less than 4 h. This study presents a novel, rapid method for accurately quantifying species in herbal products and advances the application of DNA barcoding from species identification to quantitative detection.

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Operating Room Nurses’ Role in Multidisciplinary Surgical Coordination for a Patient with a Large Abdominal Tumor and Multiple Pelvic Fractures

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Journal of Clinical and Nursing Research 2024年第5期8卷 224-232页

作者： Jing Wang Yanshu wei Xin Zhao Xuejing Li Jin pei wei Zhang Peking University People’s Hospital Beijing 100044China

in this study,we have summarized the coordination of operating room nurses participating in the multidisciplinary team in diagnosing and treating a patient with a large abdominal tumor and multiple pelvic *** perform surgical treatment on patients with various conditions,it is crucial to consider the patients from a holistic ***,the existing medical model has shifted from a“disease-centered”approach focusing on single-disciplinary diagnosis and treatment,to a“patient-centered”approach that involves multiple disciplines in diagnosis and *** room nurses,as crucial collaborators of surgeons,should make necessary adjustments to enhance their comprehension of patients,improving the overall quality of surgical coordination.

关键词： Operating room nurses Multidisciplinary team Surgical coordination

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Primary pancreatic peripheral T-cell lymphoma:A case report

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World Journal of Gastrointestinal Oncology 2024年第4期16卷 1668-1675页

作者： Yan-Liang Bai Li-Jie Wang Hui Luo Ya-Bin Cui Jin-Hui Xu Hui-Jie Nan pei-Yao Yang Jun-wei Niu Ming-Yue Shi Department of Hematology Zhengzhou University People's Hospital and Henan Provincial People's HospitalZhengzhou 450003Henan ProvinceChina Department of Hematology Henan University People's Hospital and Henan Provincial People's HospitalZhengzhou 450003Henan ProvinceChina Department of Radiation Oncology The Affiliated Cancer Hospital of Zhengzhou UniversityZhengzhou 450008Henan ProvinceChina

BACKGROUND Primary pancreatic lymphoma(PPL)is an exceedingly rare tumor with limited mention in scientific *** clinical manifestations of PPL are often nonspecific,making it challenging to distinguish this disease from other panc-reatic-related *** remains the primary treatment for these *** SUMMARY in this case study,we present the clinical details of a 62-year-old woman who initially presented with vomiting,abdominal pain,and dorsal *** further evaluation through positron emission tomography-computed tomography,the patient was considered to have a pancreatic head ***,subsequent endoscopic ultrasonography-guided fine needle aspiration(EUS-FNA)revealed that the patient had pancreatic peripheral T-cell lymphoma,not otherwise specified(PTCL-NOS).There was a substantial decrease in the size of the pancreatic mass after the patient underwent a cycle of chemotherapy comprised of brentuximab vedotin,decitabine,and oxaliplatin(brentuximab vedotin and Gemox).The patient had significant improvement in radiological findings at the end of the first *** Primary pancreatic PTCL-NOS is a malignant and heterogeneous lymphoma,in which the clinical manifestations are often *** is difficult to diagnose,and the prognosis is *** can only be used for auxiliary diagnosis of other *** the help of immunostaining,EUS-FNA could be used to aid in the diagnosis of *** a clear diagnosis,chemotherapy is still the first-line treatment for such patients,and surgical resection is not recommended.A large number of recent studies have shown that the CD30 antibody drug has potential as a therapy for several types of ***,identifying new CD30-targeted therapies for different types of lymphoma is urgently *** the future,further research on antitumor therapy should be carried out to improve the survival prognosis of such patients.

关键词： Pancreatic cancer Lymphoma Chemotherapy Primary pancreatic lymphoma Case report

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Cardiotoxicity induced by fluoropyrimidine drugs in the treatment of gastrointestinal tumors

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World Journal of Gastrointestinal Oncology 2024年第2期16卷 251-254页

作者： Mo-wei Kong Feng-Di Sun Zhen-Ying pei Li Xu Ze-Bi Wang Yan Chen Shu-Qing Tang Ting-Fang Yang Guo-Xiang He Department of Cardiology Guiqian International General HospitalGuiyang 550018Guizhou ProvinceChina Department of Oncology Guiqian International General HospitalGuiyang 550018Guizhou ProvinceChina

in this editorial,we review the article published in World J Gastrointest Oncol 2019,11:*** specifically focus on the occurrence,clinical characteristics,and risk factors of fluoropyrimidine drug-related cardiotoxicity in patients with gastrointestinal *** significant advancements in diagnostic and therapeutic techniques that have reduced mortality rates associated with digestive system tumors,the incidence and mortality rates of treatment-related car-diotoxicity have been increasing,severely impacting the survival and prognosis of cancer *** drugs are widely used as antimetabolites in the treatment of malignant tumors,including gastrointestinal tumors,and they represent the second largest class of drugs associated with ***,there is often a lack of awareness or understanding regarding their cardiotoxic effects and associated risks.

关键词： Cardiotoxicity Gastrointestinal tumors Risk factors Fluoropyrimidine Chest pain

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Heavy ion energy influence on multiple-cell upsets in small sensitive volumes:from standard to high energies

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Nuclear Science and Techniques 2024年第5期35卷 109-121页

作者： Yang Jiao Li-Hua Mo Jin-Hu Yang Yu-Zhu Liu Ya-Nan Yin Liang Wang Qi-Yu Chen Xiao-Yu Yan Shi-wei Zhao Bo Li You-Mei Sun pei-Xiong Zhao Jie Liu institute of Modern Physics Chinese Academy of ScienceLanzhou 730000China University of Chinese Academy of Science Beijing 100049China Spallation Neutron Source Science Center Dongguan 523803China China Electronics Technology Group Corporation No.58 Research institute Wuxi 214000China Beijing Microelectronics Technology institute Beijing 100084China institute of Microelectronics Chinese Academy of SciencesBeijing 100029China Key Laboratory of Science and Technology on Silicon Devices Chinese Academy of SciencesBeijing 100029China

The 28 nm process has a high cost-performance ratio and has gradually become the standard for the field of radiation-hardened ***,owing to the minimum physical gate length of only 35 nm,the physical area of a standard 6T SRAM unit is approximately 0.16μm^(2),resulting in a significant enhancement of multi-cell charge-sharing ***-cell upsets(MCUs)have become the primary physical mechanism behind single-event upsets(SEUs)in advanced nanometer node *** range of ionization track effects increases with higher ion energies,and spacecraft in orbit primarily experience SEUs caused by high-energy ***,ground accelerator experiments have mainly obtained low-energy ion irradiation ***,the impact of ion energy on the SEU cross section,charge collection mechanisms,and MCU patterns and quantities in advanced nanometer devices remains *** this study,based on the experimental platform of the Heavy ion Research Facility in Lanzhou,low-and high-energy heavy-ion beams were used to study the SEUs of 28 nm SRAM *** influence of ion energy on the charge collection processes of small-sensitive-volume devices,MCU patterns,and upset cross sections was obtained,and the applicable range of the inverse cosine law was *** findings of this study are an important guide for the accurate evaluation of SEUs in advanced nanometer devices and for the development of radiation-hardening techniques.

关键词： 28 nm static random access memory(SRAM) Energy effects Heavy ion Multiple-cell upset(MCU) Charge collection inverse cosine law

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Pectin methylesterase inhibitors GhPMEi53 and AtPMEi19 improve seed germination by modulating cell wall plasticity in cotton and Arabidopsis

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Journal of integrative Agriculture 2024年第10期23卷 3487-3505页

作者： Yayue pei Yakong Wang Zhenzhen wei Ji Liu Yonghui Li Shuya Ma Ye Wang Fuguang Li Jun Peng Zhi Wang Zhengzhou Research Base State Key Laboratory of Cotton Bio-breeding and Integrated UtilizationZhengzhou UniversityZhengzhou 450001China National Nanfan Research institute(Sanya) Chinese Academy of Agricultural SciencesSanya 572024China National Key Laboratory of Cotton Bio-breeding and integrated Utilization/institute of Cotton Research Chinese Academy ofAgricultural SciencesAnyang 455000China

The germination process of seeds is influenced by the interplay between two opposing factors,pectin methylesterase(PME)and pectin methylesterase inhibitor(PMEi),which collectively regulate patterns of pectin *** the recognized importance of pectin methylesterification in seed germination,the specific mechanisms that govern this process remain *** this study,we demonstrated that the overexpression of GhPMEi53is associated with a decrease in PME activity and an increase in pectin *** leads to seed cell wall softening,which positively regulates cotton seed ***19,the homologue in Arabidopsis thaliana,plays a similar role in seed germination to GhPMEi53,indicating a conserved function and mechanism of PMEi in seed germination *** studies revealed that GhPMEi53 and AtPMEi19 directly contribute to promoting radicle protrusion and seed germination by inducing cell wall softening and reducing mechanical ***,the pathways of abscicic acid(ABA)and gibberellin(GA)in the transgenic materials showed significant changes,suggesting that GhPMEi53/AtPMEi19-mediated pectin methylesterification serves as a regulatory signal for the related phytohormones involved in seed *** summary,GhPMEi53 and its homologs alter the mechanical properties of cell walls,which influence the mechanical resistance of the endosperm or ***,they impact cellular phytohormone pathways(e.g.,ABA and GA)to regulate seed *** findings enhance our understanding of pectin methylesterification in cellular morphological dynamics and signaling transduction,and contribute to a more comprehensive understanding of the PME/PMEi gene superfamily in plants.

关键词： cotton seed germination cell wall pectin demethylesterification PMEi ABA

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Pectin methylesterase inhibitors GhPMEi53 and AtPMEi19 improve seed germination by modulating cell wall plasticity in cotton and Arabidopsis

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Journal of integrative Agriculture 2024年第10期 3487-3505页

作者： Yayue pei Yakong Wang Zhenzhen wei Ji Liu Yonghui Li Shuya Ma Ye Wang Fuguang Li Jun Peng Zhi Wang Zhengzhou Research Base State Key Laboratory of Cotton Bio-breeding and Integrated Utilization Zhengzhou University National Nanfan Research institute (Sanya) Chinese Academy of Agricultural Sciences National Key Laboratory of Cotton Bio-breeding and integrated Utilization/institute of Cotton Research Chinese Academy of Agricultural Sciences

The germination process of seeds is influenced by the interplay between two opposing factors, pectin methylesterase(PME) and pectin methylesterase inhibitor(PMEi), which collectively regulate patterns of pectin *** the recognized importance of pectin methylesterification in seed germination, the specific mechanisms that govern this process remain unclear. in this study, we demonstrated that the overexpression of GhPMEi53is associated with a decrease in PME activity and an increase in pectin methylesterification. This leads to seed cell wall softening, which positively regulates cotton seed germination. AtPMEi19, the homologue in Arabidopsis thaliana, plays a similar role in seed germination to GhPMEi53, indicating a conserved function and mechanism of PMEi in seed germination regulation. Further studies revealed that GhPMEi53 and AtPMEi19 directly contribute to promoting radicle protrusion and seed germination by inducing cell wall softening and reducing mechanical ***, the pathways of abscicic acid(ABA) and gibberellin(GA) in the transgenic materials showed significant changes, suggesting that GhPMEi53/AtPMEi19-mediated pectin methylesterification serves as a regulatory signal for the related phytohormones involved in seed germination. in summary, GhPMEi53 and its homologs alter the mechanical properties of cell walls, which influence the mechanical resistance of the endosperm or ***, they impact cellular phytohormone pathways(e.g., ABA and GA) to regulate seed germination. These findings enhance our understanding of pectin methylesterification in cellular morphological dynamics and signaling transduction, and contribute to a more comprehensive understanding of the PME/PMEi gene superfamily in plants.

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Roles of NAC transcription factors in cotton

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Journal of Cotton Research 2024年第3期 289-301页

作者： XU Yuewei ZHAO Yunlei CHEN wei SANG Xiaohui ZHAO pei WANG Hongmei Zhengzhou Research Base National Key Laboratory of Cotton Bio-breeding and Integrated Utilization Zhengzhou University National Key Laboratory of Cotton Bio-breeding and integrated Utilization Institute of Cotton Research Chinese Academy of Agricultural Sciences

Climate deterioration, water shortages, and abiotic stress are the main threats worldwide that seriously affect cotton growth, yield, and fiber quality. Therefore, research on improving cotton yield and tolerance to biotic and abiotic stresses is of great importance. The NAC proteins are crucial and plant-specific transcription factors(TFs) that are involved in cotton growth, development, and stress responses. The comprehensive utilization of cotton NAC TFs in the improvement of cotton varieties through novel biotechnological methods is feasible. Based on cotton genomic data, genome-wide identification and analyses have revealed potential functions of cotton NAC genes. Here, we comprehensively summarize the recent progress in understanding cotton NAC TFs roles in regulating responses to drought, salt, and Verticillium wilt-related stresses, as well as leaf senescence and the development of fibers, xylem, and glands. The detailed regulatory network of NAC proteins in cotton is also elucidated. Cotton NAC TFs directly bind to the promoters of genes associated with ABA biosynthesis and secondary cell-wall formation, participate in several biological processes by interacting with related proteins, and regulate the expression of downstream genes. Studies have shown that the overexpression of NAC TF genes in cotton and other model plants improve their drought or salt tolerance. This review elucidates the latest findings on the functions and regulation of cotton NAC proteins, broadens our understanding of cotton NAC TFs, and lays a fundamental foundation for further molecular breeding research in cotton.

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制度逻辑间的非兼容性与基层市场监管的变通执行

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深圳大学学报（人文社会科学版） 2024年第1期41卷 94-102页

作者：刘培伟山东农业大学公共管理学院山东泰安271018

对“双随机、一公开”制度执行状况的考察,不仅关涉对基层监管行为逻辑的认识,也关乎对国家治理理性化进程的判断。S县市场监管局执行该制度的过程显示,因该制度所基于的现代性治理逻辑难以兼容于国家的总体性治理逻辑,致使其无意也无... 详细信息

对“双随机、一公开”制度执行状况的考察,不仅关涉对基层监管行为逻辑的认识,也关乎对国家治理理性化进程的判断。S县市场监管局执行该制度的过程显示,因该制度所基于的现代性治理逻辑难以兼容于国家的总体性治理逻辑,致使其无意也无力将“双随机”作为基本监管方式;因要服从于地方政府的稳定和发展“大局”,又使其不可能按规定公开处罚信息。基层监管部门因受制于国家层面和地方层面多重且难以兼容的制度逻辑的约束,尤其是受“地方大局”逻辑的支配,其行为并不遵循一个从自利到为避责而恪守程序的线性逻辑,而是有着多重多变的面向。基层的变通执行最终导致了两个“非意图后果”:因规避问责而有疏漏或掩盖真实风险的可能,因追求经济达标而背离国家监管结构优化的可能。解决基层市场监管变通问题的根本在于优化政府职责体系,理顺央地政府之间以及地方政府与社会和市场之间的关系,使监管人员有动机有能力执行国家的理性化制度设计,切实推动治理的理性化和现代化。

关键词：市场监管变通执行制度逻辑非兼容性总体性治理现代性治理非意图后果

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Probing multi-physical process and deformation mechanism of a largescale landslide using integrated dual-source monitoring

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Geoscience Frontiers 2024年第2期15卷 417-432页

作者： Hong-Hu Zhu Xiao Ye Hua-Fu pei wei Zhang Gang Cheng Zi-Li Li School of Earth Sciences and Engineering Nanjing UniversityNanjing 210023China Department of Geotechnical Engineering Dalian University of TechnologyDalian 116024China Civil Structural&Environmental EngineeringSchool of Engineering and ArchitectureUniversity College CorkCorkIreland Department of Civil&Environmental Engineering Massachusetts Institute of TechnologyCambridgeMA 02139-4307United States

The implementation of isolated heterologous monitoring systems for spatially distant borehole deployments often comes with substantial equipment costs,which can limit the effectiveness of geohazard mitigation and georisk management *** address this,we have developed a novel monitoring system that integrates fiber Bragg grating(FBG)and microelectromechanical system(MEMS)techniques to capture soil moisture,temperature,sliding resistance,strain,surface tilt,and deep-seated *** system enables real-time,simultaneous data acquisition and cross-validation analyses,offering a costeffective solution for monitoring critical parameters in geohazard-prone *** successfully applied this integrated monitoring system to the Xinpu landslide,an active super-large landslide located in the Three Gorges Reservoir Area(TGRA)of *** resulting strain profile confirmed the presence of two shallow secondary sliding surfaces at depths of approximately 7 m and 12 m,respectively,in addition to the deep-seated sliding surface at a depth of28 *** lower secondary sliding surface was activated by extreme precipitation,while the upper one was primarily driven by significant changes in reservoir water levels and secondarily triggered by concentrated ***-slide piles have remarkably reinforced the upper moving masses but failed to control the lower *** gap between the pile heads and the soil amplified the rainwater erosion effect,creating a preferential channel for rainwater ***-physical measurements revealed a mixture of seepage-driven and buoyancy-driven behaviors within the *** study offers an integrated dual-source multi-physical monitoring paradigm that enables collaborative management of multiple crucial boreholes on a large-scale landslide,and contributes to the evaluation and improvement of engineering measures in similar geological settings.

关键词： Reservoir landslide Multi-physical process integrated dual-source monitoring Fiber optic Extreme weather

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