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A Virus-type Specific Serological Diagnosis of Flavivirus Infection Using Virus-like Particles

Virologica Sinica 2009年第2期24卷 136-145页

作者： Min QING Zhi-ming YUAN pei-yong shi Wadsworth Center New York State Department of Health 120 New Scotland Avenue Albany New York 12208USA Stake Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan430071 China Department of Biomedical Sciences University at Albany State University of New YorkAlbany New York Albany New York 12208 USA

Many flaviviruses are emerging and reemerging pathogens, such as West Nile virus （WNV）, dengue virus （DENV）, yellow fever virus （YFV）, and Japanese encephalitis virus. Serological assay is the dominant method for diagnosis of flavivirus infections in human. Because antibodies generated during flavivirus infections cross-react with other flavivirus members, plaque reduction neutralization test （PRNT） is the only available assay to determine the infecting flavivirus type. Since PRNT requires culturing raw viruses, it must be performed in biosafety levet-3 or level-4 containment for many flaviviruses, and takes more than ten days to complete. To overcome these problems, we have developed flavivirus viral-like particles （VLPs） that could be used to replace raw viruses in the neutralization assay. The VLPs were prepared by trans packaging a luciferase-reporting replicon with viral structural proteins. This novel assay involves three simple steps：（i） VLPs from a panel of flaviviruses are incubated with flavivirus-infected sera at 37℃ for 1 h; （ii）the neutralized VLPs are used to infect Vero cells; and （iii） the infected cells are measured for luciferase activities at 22 h post-infection. The virus type whose VLP is most efficiently neutralized by the serum specimen （as quantified by the luciferase activities） is the etiologic agent. As a proof-of-concept, we show that a WNV-infected mouse serum neutralized the WNV VLP more efficiently and selectively than the DENV and YFV VLPs. Our results demonstrate that the VLP neutralization assay maintains the ＂gold standard＂ of the classic PRNT; importantly, it shortens the assay time from 〉10 days to 〈1 day, and can be performed in biosafety level-2 facility.

关键词： West Nile virus Neutralization assay Viral-like particle Serological diagnosis Flavivirus packaging

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Report of the Fifth Symposium on Emerging Viral Diseases

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Virologica Sinica 2013年第2期28卷 63-64页

作者： Bo Zhang pei-yong shi State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Novartis Institute for Tropical Diseases

Emerging viral diseases pose global threat to public health. It is essential to build capacity to response and counteract against emerging viral diseases. Towards achieving this important goal of public health, the St... 详细信息

关键词：病毒性疾病中国科学院武汉病毒研究所新生国家重点实验室国际研讨会公众健康能力建设公共卫生

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Zika virus： a new threat from mosquitoes

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Science China(Life Sciences) 2016年第4期59卷 440-442页

作者： Xiao-Feng Li Jian-Feng Han pei-yong shi Cheng-Feng Qin Department of Virology Beijing Institute of Microbiology and Epidemiology Beijing 100071 China. State Key Laboratory of Pathogen and Biosecurity Beijing 100071 China. Department of Biochemistry and Molecular Biology Department of Phamarcology & Toxicology Sealy Center for Structural Biology & Molecular Biophysics University of Texas Medical Branch Galveston Texas 77555 USA

In the winter of 2015,the first dengue vaccine(Dengvaxia)was approved for clinical *** virus is the most prevalent mosquito-transmitted viral pathogen in humans(Qin and shi,2014).Even though the approved Dengvaxia has... 详细信息

关键词： threat approved serotype licensed dengue Zika virus weaknesses winter vaccine transmitted

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Ubiquitination of SARS-CoV-2 ORF7a promotes antagonism of interferon response

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Cellular & Molecular Immunology 2021年第3期18卷 746-748页

作者： Zengguo Cao Hongjie Xia Ricardo Rajsbaum Xianzhu Xia Hualei Wang pei-yong shi Key Laboratory of Zoonosis Research Ministry of EducationCollege of Veterinary MedicineJilin UniversityChangchun 130062China Department of Biochemistry and Molecular Biology University of Texas Medical BranchGalvestonTX 77555USA Department of Microbiology and Immunology University of Texas Medical BranchGalvestonTX 77555USA lnstitute for Human Infections and Immunity University of Texas Medical BranchGalvestonTX 77555USA Sealy Institute for Vaccine Sciences University of Texas Medical BranchGalvestonTX 77555USA Sealy Center for Structural Biology&Molecular Biophysics University of Texas Medical BranchGalvestonTX 77555USA

Understanding interactions between the host and SARS-CoV-2 is essential for developing effective vaccines and ***,we report that SARS-CoV-2 usurps the host ubiquitin system to polyubiquitinate accessory protein ORF7a ... 详细信息

关键词： ORF7 interferon primarily

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Flavivirus RNA cap methyltransferase:structure,function,and inhibition

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Frontiers in Biology 2010年第4期5卷 286-303页

作者： Lihui LIU Hongping DONG Hui CHEN Jing ZHANG Hua LING Zhong LI pei-yong shi Hongmin LI Wadsworth Center New York State Department of Health120 New Scotland AveAlbanyNY 12208USA Novartis Institute for Tropical Diseases 10 Biopolis RoadSingapore 138670 Department of Biomedical Sciences School of Public HealthState University of New YorkAlbanyNew York 12201-0509USA

Many flaviviruses are significant human *** plus-strand RNA genome of a flavivirus contains a 5′terminal cap 1 structure(m7GpppAmG).The flavivirus encodes one methyltransferase(MTase),located at the Nterminal portion of the NS5 RNA-dependent RNA polymerase(RdRp).Here we review recent advances in our understanding of flaviviral capping machinery and the implications for drug *** NS5 MTase catalyzes both guanine N7 and ribose 2'-OH methylations during viral cap *** flavivirus MTases,from dengue,yellow fever,and West Nile virus(WNV),sequentially generate GpppA!m7GpppA!*** the existence of two distinct methylation activities,the crystal structures of flavivirus MTases showed a single binding site for S-adenosyl-L-methionine(SAM),the methyl *** finding indicates that the substrate GpppA-RNA must be repositioned to accept the N7 and 2'-O methyl groups from SAM during the sequential *** studies demonstrated that distinct RNA elements are required for the methylations of guanine N7 on the cap and of ribose 2'-OH on the first transcribed *** enzymes with different methylation defects can trans complement one another in vitro,demonstrating that separate molecules of the enzyme can independently catalyze the two cap methylations in *** the context of the infectious virus,defects in both methylations,or a defect in the N7 methylation alone,are lethal to ***,viruses defective solely in 2'-O methylation are attenuated and can protect mice from later wild-typeWNV *** results demonstrate that the N7 methylation activity is essential for the WNV life cycle and,thus,methyltransferase represents a novel and promising target for flavivirus therapy.

关键词： Flavivirus NS5 RNA cap methylation methyltransferase structure and function inhibitor

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Concise extraction and characterization of the pore-throat network in unconventional hydrocarbon reservoirs: A new perspective

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Petroleum Science 2024年第3期21卷 1474-1487页

作者： Shu-Heng Du yong-Min shi State Key Laboratory of Nonlinear Mechanics Institute of MechanicsChinese Academy of SciencesBeijing100190China School of Engineering Science University of Chinese Academy of SciencesBeijing100049China School of Earth and Space Sciences Peking UniversityBeijing100871China

In this study,a new image-based method for the extraction and characterization of pore-throat network for unconventional hydrocarbon storage and exploitation is proposed.“Pore-throat solidity”,which is analogous to particle solidity,and a new method for automatic identification of pores and throats in tight sandstone oil reservoirs are ***,the“pore-throat combination”and“pure pore”are defined and distinguished by drawing the cumulative probability curve of the pore-throat solidity and by selecting an appropriate cutoff *** the discrete grid set is recognized as a pore-throat combination,Legendre ellipse fitting and minimum Feret diameter are *** the pore and throat grid sets are identified as pure pores,the pore diameter can be directly *** the new method,the analytical results for the physical parameters and pore radius agree well with most prior *** results comparing the maximum ball and the new model could also prove the accuracy of the latter's in micro and nano *** new model provides a more practical theoretical basis and a new calculation method for the rapid and accurate evaluation of the complex processes of oil migration.

关键词： Hydrocarbon exploitation Pore Throat Porous media Identification

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A novel weld-pool-length monitoring method based on pixel analysis in plasma arc additive manufacturing

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Advances in Manufacturing 2024年第2期12卷 335-348页

作者： Bao-Ri Zhang yong-Hua shi Bionic Robot Research Department Ji Hua LaboratoryFoshan528200GuangdongPeople’s Republic of China Guangdong Provincial Engineering Research Center for Special Welding Technology and Equipment School of Mechanical and Automotive EngineeringSouth China University of TechnologyGuangzhou510640People’s Republic of China

The real-time monitoring of the weld pool during deposition is important for automatic control in plasma arc additive *** obtain a high deposition accuracy,it is essential to maintain a stable weld pool *** this study,a novel passive visual method is proposed to measure the weld pool *** the proposed method,the image quality was improved by designing a special visual system that employed an endoscope and a *** also includes pixel brightness-based and gradient-based algorithms that can adaptively detect feature points at the boundary when the weld pool geometry *** algorithm can also be applied to materials with different solidification *** was performed to measure the real weld pool length in world coordinates,and outlier rejection was performed to increase the accuracy of the ***,tests were carried out on the intersection component,and the results showed that the proposed method performed well in tracking the changing weld pool length and was applicable to the real-time monitoring of different types of materials.

关键词： Plasma arc additive manufacturing(PAAM) Weld pool geometry Gradient analysis Real-time detection

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Oxygen vacancy boosting Fenton reaction in bone scaffold towards fighting bacterial infection

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International Journal of Extreme Manufacturing 2024年第1期6卷 296-311页

作者： Cijun Shuai Xiaoxin shi Feng Yang Haifeng Tian pei Feng State Key Laboratory of Precision Manufacturing for Extreme Service Performance College of Mechanical and Electrical EngineeringCentral South UniversityChangsha 410083People’s Republic of China Institute of Additive Manufacturing Jiangxi University of Science and TechnologyNanchang 330013People’s Republic of China College of Mechanical Engineering Xinjiang UniversityUrumqi 830017People’s Republic of China

Bacterial infection is a major issue after artificial bone transplantation due to the absence of antibacterial function of bone scaffold,which seriously causes the transplant failure and even amputation in severe *** this study,oxygen vacancy(OV)defects Fe-doped Ti O2(OV-FeTiO2)nanoparticles were synthesized by nano TiO2and Fe3O4via high-energy ball milling,which was then incorporated into polycaprolactone/polyglycolic acid(PCLGA)biodegradable polymer matrix to construct composite bone scaffold with good antibacterial activities by selective laser *** results indicated that OV defects were introduced into the core/shell-structured OV-FeTiO2nanoparticles through multiple welding and breaking during the high-energy ball milling,which facilitated the adsorption of hydrogen peroxide(H2O2)in the bacterial infection microenvironment at the bone transplant *** accumulated H2O2could amplify the Fenton reaction efficiency to induce more hydroxyl radicals(·OH),thereby resulting in more bacterial deaths through·OH-mediated oxidative *** antibacterial strategy had more effective broad-spectrum antibacterial properties against Gram-negative Escherichia coli(***)and Gram-positive Staphylococcus aureus(***).In addition,the PCLGA/OV-FeTiO2scaffold possessed mechanical properties that match those of human cancellous bone and good biocompatibility including cell attachment,proliferation and osteogenic differentiation.

关键词： bacterial infection bone scaffold selective laser sintering Fenton reaction antibacterial properties

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Ag-doped CNT/HAP nanohybrids in a PLLA bone scaffold show significant antibacterial activity

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Bio-Design and Manufacturing 2024年第2期7卷 105-120页

作者： Cijun Shuai Xiaoxin shi Kai Wang Yulong Gu Feng Yang pei Feng State Key Laboratory of Precision Manufacturing for Extreme Service Performance College of Mechanical and Electrical EngineeringCentral South UniversityChangsha 410083China Institute of Additive Manufacturing Jiangxi University of Science and TechnologyNanchang 330013China College of Mechanical Engineering Xinjiang UniversityUrumqi 830017China

Bacterial infection is a major problem following bone implant ***,poly-l-lactic acid/carbon nanotube/hydroxyapatite(PLLA/CNT/HAP)bone scaffolds possess enhanced mechanical properties and show good ***,wesynthesizedsilver(Ag)-dopedCNT/HAP(CNT/Ag-HAP)nanohybrids via the partial replacing of calcium ions(Ca2+)in the HAP lattice with silver ions(Ag+)using an ion doping technique under hydrothermal ***ﬁcally,the doping process was induced using the special lattice structure of HAP and the abundant surface oxygenic functional groups of CNT,and involved the partial replacement of Ca2+in the HAP lattice by doped Ag+as well as the in situ synthesis of Ag-HAP nanoparticles on CNT in a hydrothermal *** result-ing CNT/Ag-HAP nanohybrids were then introduced into a PLLA matrix via laser-based powder bed fusion(PBF-LB)to fabricate PLLA/CNT/Ag-HAP scaffolds that showed sustained antibacterial *** then found that Ag+,which pos-sesses broad-spectrum antibacterial activity,endowed PLLA/CNT/Ag-HAP scaffolds with this activity,with an antibacterial effectiveness of 92.65%.This antibacterial effect is due to the powerful effect of Ag+against bacterial structure and genetic material,as well as the physical destruction of bacterial structures due to the sharp edge structure of *** addition,the scaffold possessed enhanced mechanical properties,showing tensile and compressive strengths of 8.49 MPa and 19.72 MPa,***,the scaffold also exhibited good bioactivity and cytocompatibility,including the ability to form apatite layers and to promote the adhesion and proliferation of human osteoblast-like cells(MG63 cells).

关键词： Ag-doped Carbon nanotube/hydroxyapatite(CNT/HAP) Antibacterial properties Bone scaffold

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Molecular Dynamics Simulation Studies on the Micromorphology and Proton Transport of Nafion/Ti_(3)C_(2)T_(x) Composite Membrane

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Chinese Journal of Polymer Science 2024年第3期42卷 373-387,I0009页

作者： Zhi-Yue Han Su-Peng pei Chun-Yang Yu yong-Feng Zhou School of Chemical and Environmental Engineering Shanghai Institute of TechnologyShanghai 201418China School of Chemistry&Chemical Engineering Frontiers Science Center for Transformative MoleculesShanghai Key Laboratory of Electrical Insulation and Thermal AgingShanghai Jiao Tong UniversityShanghai 200240China Key Laboratory of Green and High-end Utilization of Salt Lake Resources Chinese Academy of SciencesXining 810008China

The perfluorosulfonic acid(PFSA) membrane doped with two-dimensional conductive filler Ti_(3)C_(2)T_(x) is a fuel cell proton exchange membrane with high application potential. Experimental studies showed that the proton conductivity of Nafion/Ti_(3)C_(2)T_(x) composite membrane is improved significantly compared with that in pure Nafion. However, the microscopic mechanism of doping on the enhancement of membrane performance is remain unclear now. In this work, molecular dynamics simulation was used to investigate the microscopic morphology and proton transport behaviors of Nafion/Ti_(3)C_(2)T_(x) composite membrane at the molecular level. The results shown that there were significant differences about the diffusion kinetics of water molecules and hydroxium ions in Nafion/Ti_(3)C_(2)T_(x) at low and high hydration levels in the nanoscale *** the increase of water content, Ti_(3)C_(2)T_(x) in membrane was gradually surrounded by ambient water molecules to form a hydration layer, and forming a relatively continuous proton transport channel between Nafion polymer and Ti_(3)C_(2)T_(x) monomer. The continuous proton transport channel could increase the number of binding sites of proton and thus achieving high proton conductivity and high mobility of water molecules at higher hydration level. The current work can provide a theoretical guidance for designing new type of Nafion composite membranes.

关键词： Nafion/Ti_(3)C_(2)T_(x)composite membrane Proton transport Molecular dynamics simulation

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