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Overexpression of TRPV1 activates autophagy in human lens epithelial cells under hyperosmotic stress through Ca^(2+)-dependent AMPK/mTOR pathway

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International Journal of Ophthalmology(English edition) 2024年第3期17卷 420-434页

作者： Liu-Hui Huang Jiao Lyu Sheng Chen Ting-Yi Liang Yu-Qing Rao Ping Fei Jing Li Hai-Ying Jin pei-quan zhao Department of Ophthalmology Shanghai Xinhua Hospital Affiliated with Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Ophthalmology Shanghai Tenth People’s Hospital Affiliated with Shanghai Tongji University School of MedicineShanghai 200072China Centre for Eye Research Australia Royal Victorian Eye and Ear HospitalEast MelbourneVictoria 3002Australia Ophthalmology Department of SurgeryUniversity of MelbourneEast MelbourneVictoria 3002Australia Shenzhen Eye Hospital Shenzhen Key Laboratory of OphthalmologyAffiliated Shenzhen Eye Hospital of Jinan UniversityShenzhen 518040Guangdong ProvinceChina Department of Ophthalmology Shanghai East HospitalSchool of MedicineTongji UniversityShanghai 200120China

●AIM:To explore whether autophagy functions as a cellular adaptation mechanism in lens epithelial cells(LECs)under hyperosmotic stress.●METHODS:LECs were treated with hyperosmotic stress at the concentration of 270,300,400,500,or 600 mOsm for 6,12,18,24h in *** chain reaction(PCR)was employed for the mRNA expression of autophagyrelated genes,while Western blotting detected the targeted protein *** transfection of stub-RFP-sens-GFPLC3 autophagy-related double fluorescence lentivirus was conducted to detect the level of autophagy *** electron microscopy was used to detect the existence of *** interfering RNA of autophagy-related gene(ATG)7,transient receptor potential vanilloid(TRPV)1 overexpression plasmid,related agonists and inhibitors were employed to their influence on autophagy related *** cytometry was employed to test the apoptosis and intracellular Ca^(2+)*** membrane potential was measured by JC-1 *** cell counting kit-8 assay was used to calculate the cellular *** wound healing assay was used to evaluate the wound closure *** 6.0 software was utilized to evaluate the data.●RESULTS:The hyperosmotic stress activated autophagy in a pressure-and time-dependent manner in *** 1 protein expression and conversion of LC3B II to LC3B I increased,whereas sequestosome-1(SQSTM1)protein expression *** Ca^(2+)influx was stimulated caused by hyperosmotic stress,levels of mammalian target of rapamycin(mTOR)phosphorylation decreased,and the level of AMP-activated protein kinase(AMPK)phosphorylation increased in the early *** on this evidence,autophagy activation through the Ca^(2+)-dependent AMPK/mTOR pathway might represent an adaptation process in LECs under hyperosmotic *** stress decreased cellular viability and accelerated apoptosis in LECs and cellular migration *** of autophagy by ATG7 knockdown had sim

关键词： cataract posterior capsular opacification lens epithelial cell hyperosmotic stress autophagy apoptosis transient receptor potential vanilloid 1

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Clinical observations of vitreoretinal surgery for four different phenotypes of X-linked congenital retinoschisis

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International Journal of Ophthalmology(English edition) 2018年第6期11卷 986-990页

作者： Chen zhao Qi Zhang Hai-Ying Jin pei-quan zhao Department of Ophthalmology Xinhua Hospital School of Medicine Shanghai Jiao Tong University

AIM： To evaluate the outcomes of vitreoretinal surgery for four different phenotypes of X-linked retinoschisis （XLRS）. METHODS： This study included thirty-one eyes of 25 patients who developed XLRS with severe complications. Among the 31 eyes, there were 7 eyes with vitreous hemorrhage, 8 eyes with retinal detachment and vitreous hemorrhage, and 16 eyes with rhegmatogenous retinal detachment. All of the patients underwent standard three-port pars plana vitrectomy. All of the eyes were divided into 4 groups before surgery according to a modified classification scheme, with the first three groups as follows： group A, with foveal cystic schisis; group B with macular lamellar schisis; and group C with foveolamellar changes. Peripheral schisis was not present in these 3 groups; however, group D was a complex group with both macular and peripheral changes. One year after surgery, we analyzed the best corrected visual acuity and postoperative anatomical and functional outcomes of these 4 groups. RESULTS： There were 7 eyes in group A （22.6%）, 1 eye in group B （3.2%）, 15 eyes in group C （48.4%） and 8 eyes in group D （25.8%）. Postoperative anatomical and functional outcomes were satisfactory at the last visit, while the mean visual acuity was increased to 0.27±0.11, with a significant difference （t=-1.049, P=0.000） compared with the results before surgery （0.1±0.08）. Visual acuity was improved in 23 eyes （74.2%）. Complications were found in three eyes： two eyes with proliferative vitreoretinopathy and traction retinal detachment 10 and 12mo after surgery, respectively; and one eye with vitreous hemorrhage 15mo after surgery. These eyes were in groups C and D. The retinas remained attached in these 3 eyes after reoperation. CONCLUSION： We should pay greater attention to XLRS with foveolamellar changes because it is the most changeable phenotype. Once complications occur, vitreoretinal surgery can significantly improve visual acuity and restore the anatomic structur

关键词： X-linked retinoschisis complications vitreoretinal surgery

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Clinical features of posterior microphthalmic and nanophthalmic eyes

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International Journal of Ophthalmology(English edition) 2018年第11期11卷 1829-1834页

作者： Jing-Jing Liu Yi-Ye Chen Xiang Zhang pei-quan zhao Department of Ophthalmology Xin Hua Hospital Shanghai Jiao Tong University School of Medicine

AIM： To clinically differentiate nanophthalmos（NO） and posterior microphthalmos（PM） and to explore the mechanisms related to papillomacular folds（PMF）.METHODS： Medical records of 34 unrelated patients with microphthalmos（54 eyes） from April 2009 to October 2017 were retrospectively ***： Fourteen eyes of 7 unrelated patients with NO and PM were included in the study. The presenting age of the NO cohort was significantly higher compared with the PM cohort（NO： 27±16 y; PM： 3.7±0.6 y）. PMF was more likely to occur in cases with PM than in NO（25% in NO, 100% in PM）. The anatomic features of PMF from optical coherence tomography（OCT） included： ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer and outer nuclear layer. In eyes without an apparent PMF（these were all NO eyes）, rudimentary fovea without a foveal pit was noted. Four eyes that were NO developed angle closure glaucoma. Three NO eyes developed exudative retinal detachment and were successfully treated with lamellar ***： Posterior segment changes are pervasive both in PM and NO. Complications like angle closure glaucoma and exudative retinal detachment are likely to occur in eyes with NO but not with PM. Detailed OCT analysis found that PMF was partially a neural retinal issue, suggesting that redundancy of retinal issues involved only inner retinal layers.

关键词： nanophthalmos non-rhegmatogenous retinal detachment optical coherence tomography papillomacular folds posterior microphthalmos rudimentary fovea

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Analyzing cytokines as biomarkers to evaluate severity of glaucoma

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International Journal of Ophthalmology(English edition) 2017年第6期10卷 925-930页

作者： Yao Tong Ya-Li Zhou Yan Zheng Manas Biswal pei-quan zhao zhao-Yang Wang Department of Ophthalmology Shanghai Ninth People＇s Hospital Shanghai Jiaotong University School of Medicine Shanghai 200011 China Department of Ophthalmology Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200092 China Department of Ophthalmology Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine Chongming Branch Shanghai 202150 China Department of Molecular Genetics University of Florida Gainesville Florida 32610 USA

AIM： To analyze cytokines as biomarkers for evaluation of severity of glaucoma. METHODS： This was a prospective case-control study including 29 eyes with glaucoma. Besides, 28 eyes with senile cataract were used as control. Patients were classified into four groups： acute angle closure glaucoma （AACG）, chronic angle closure glaucoma （CACG）, primary open angle glaucoma （POAG） and senile cataract. Undiluted vitreous samples were collected, then vitreous concentrations of 9 types of cytokines were determined by cytometric bead assay system： y-interferon （IFNg）, interleukin （IL-10, IL-2, IL-4, IL-5, interferon-y-inducible protein （IP）-10, monocyte chemoattractant protein （MCP）-I, tumor necrosis factor （TNF） -Q, and vascular endothelial growth factor （VEGF）. We also recorded the intraocular pressure （lOP） of patients in each group and Pearson correlated analysis was performed to analysis the correlation between each type of cytokine with IOR RESULTS： Vitreous levels of IL-2, iL-5, MCP-1, TNF-a and IP-10 were significantly higher （P〈0.05） in AACG group. Patients with AACG, CACG and POAG have higher lOP than senile cataract, but we didn＇t find any significant correlation between lOP with any type of the cytokines. CONCLUSION： Inflammation and immune reaction have a strong link with the pathology of glaucoma especially AACG, Some cytokines may act as biomarkers to evaluate the severity of glaucoma, Anti-inflammatory treatments and controlling of lOP are necessary for the therapy of glaucoma.

关键词： glaucoma cytokines intraocular pressure

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Comparison of the efficacy of anti-VEGF monotherapy versus PDT and intravitreal anti-VEGF combination treatment in AMD： a Meta-analysis and systematic review

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International Journal of Ophthalmology(English edition) 2016年第7期9卷 1028-1037页

作者： Yao Tong Ke-ke zhao Dong Feng Manas Biswal pei-quan zhao zhao-Yang Wang Yun Zhang Eye Hospital of Wenzhou Medical University Wenzhou 325027 Zhejiang Province China Department of Ophthalmology Xinhua Hospital Shanghai Jiaotong University School of Medicine Shanghai 200092China Department of Ophthalmology Shanghai Children's Medical Center Shanghai Jiaotong University School of MedicineShanghai 200127 China Department of Ophthalmology The First Affiliated Hospital of College of Medicine Zhejiang University Hangzhou 310006 Zhejiang Province China Department of Molecular Genetics University of FloridaGainesville 32610 Florida USA

AIM：To compare the effect of anti-vascular endothelial growth factor（VEGF） monotherapy versus photodynamic therapy（PDT） and anti-VEGF combination treatment in age-related macular degeneration（AMD）.METHODS：A computerized online search was performed using PubMed,Web of Science and the Cochrane *** that compared anti-VEGF monotherapy with PDT and anti-VEGF combination treatment of AMD and were designed as randomized controlled trials were *** means and standard deviations of the best-corrected visual acuity（BCVA）,central retinal thickness（CRT）,number of treatments and proportions of patients who gained BCVA ≥15,10,5,or 0 letters at 12^（th） month were extracted.A systematic review and Meta-analysis of the comparison of the two approaches was conducted using Review Manager ***.A sensitivity analysis was also ***：Eight studies were *** the subgroup and sensitivity analysis was conducted,the results indicated that in the findings that included the monotherapy group and PDT（standard fluence,SF）group of Kaiser＇s study,the patients in the monotherapy group had a better BCVA compared with the combination group at 12^（th） month in the PDT（SF） subgroup[weighted mean difference（WMD）：3.54;95% Cl：0.36 to 6.73;P=0.03],and there were more patients who gained ≥15 letters of BCVA in the monotherapy group compared with the combination group in the total result[odds ratio（OR）：1.41;95% Cl：1.02 to 1.95;P=0.04].The same conclusion was obtained in the total result that included the monotherapy group and PDT（reduced fluence,RF）group of Kaiser＇s study（OR：1.56;95% CI：1.13 to 2.15;P=0.007）.However,there were no significant differences in the other indexes between the two ***：We found that anti-VEGF monotherapy is more effective on the recovery of visual acuity than combination therapy and more researches with lager sample size should be performed to study on the effect of the two therapy approaches on CRT and number of inject

关键词： age-related macular degeneration antivascular endothelial growth factor photodynamic therapy Meta-analysis

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Comprehensive analysis of genetic variations in strictly-defined Leber congenital amaurosis with whole-exome sequencing in Chinese

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International Journal of Ophthalmology(English edition) 2016年第9期9卷 1260-1264页

作者： Shi-Yuan Wang Qi Zhang Xiang Zhang pei-quan zhao Department of Ophthalmology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

AIM：To make a comprehensive analysis of the potential pathogenic genes related with Leber congenital amaurosis（LCA） in ***：LCA subjects and their families were retrospectively collected from 2013 to ***,whole-exome sequencing was performed in patients who had underwent gene mutation screening with nothing found,and then homozygous sites was selected,candidate sites were annotated,and pathogenic analysis was conducted using softwares including Sorting Tolerant from Intolerant（SIFT）,Polyphen-2,Mutation assessor,Condel,and Functional Analysis through Hidden Markov Models（FATHMM）.Furthermore,Gene Ontology function and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of pathogenic genes were performed followed by co-segregation analysis using Fisher exact *** sequencing was used to validate single-nucleotide variations（SNVs）.Expanded verification was performed in the rest ***：Totally 51 LCA families with 53 patients and24 family members were recruited.A total of 104 SNVs（66 LCA-related genes and 15 co-segregated genes）were submitted for expand *** frequencies of homozygous mutation of KRT12 and CYP1A1 were simultaneously observed in 3 *** analysis showed that the potential pathogenic genes were mainly enriched in functions related to cell adhesion,biological adhesion,retinoid metabolic process,and eye development biological ***,WFS7 and STAU2 had the highest homozygous ***：LCA is a highly heterogeneous *** in KRT12,CVP1A1,WFS1,and STAU2 may be involved in the development of LCA.

关键词： Leber congenital amaurosis whole-exome sequencing targeted next-generation sequencing

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Analysis of the etiologies,treatments and prognoses in children and adolescent vitreous hemorrhage

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International Journal of Ophthalmology(English edition) 2021年第2期14卷 299-305页

作者： Zheng-Wei Liu Jie Peng Chun-Li Chen Xue-Hao Cui pei-quan zhao Department of Ophthalmology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Ophthalmology Shanghai Baoshan District Wusong Central Hospital(Zhongshan Hospital Wusong BranchFudan University)Shanghai 200940China Department of Ophthalmology Beijing Tongren HospitalCapital Medical UniversityBeijing 100005China Department of Ophthalmology Tianjin Medical University Eye HospitalTianjin 300000China

AIM:To determine the etiologies,treatment modalities and visual outcomes of vitreous hemorrhage(VH;range from birth to 18 y).METHODS:A total of 262 eyes from 210 patients between January 2010 and September 2016 were *** children underwent an appropriate ocular and systemic *** collected included demographics,clinical manifestations,details of the ocular and systemic examination,management details,final fundus anatomy and visual acuity(VA).RESULTS:The most common etiologies were nontraumatic VH(64.89%),most of which were due to retinopathy of prematurity(ROP;37.10%);while traffic accidents,including 16(21.00%)eyes,was the most common ocular ***,performed in 143(54.58%)eyes,was the most common management *** initial mean baseline visual acuity was 2.77±0.21 logarithm of the minimal angle of resolution(log MAR)in children and adolescent with traumatic VH,which was significantly improved to 2.15±1.31 log MAR(P<0.05).CONCLUSION:VH in children and adolescent has a complicated and diverse *** is the primary cause of non-traumatic VH,which is the most common *** treatment of traumatic VH is associated with obvious improvement in visual *** initial VA is one of most important predictors of outcome.

关键词： etiologies visual acuity vitreous hemorrhage non-traumatic vitreous hemorrhage traumatic vitreous hemorrhage

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Combined intra-arterial chemotherapy and intravitreal melphalan for the treatment of advanced unilateral retinoblastoma

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International Journal of Ophthalmology(English edition) 2020年第2期13卷 257-262页

作者： Ting-Yi Liang Xiu-Yu Zhu Xu-Ming Hua Xun-Da Ji pei-quan zhao Department of Ophthalmology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Neurosurgery Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China

●AIM:To evaluate the efficacy and safety of combined intraarterial chemotherapy(IAC)and intravitreal melphalan(IVM)for the treatment of advanced unilateral retinoblastoma.●METHODS:This retrospective study involved 30 consecutive eyes from 30 Chinese patients with advanced unilateral *** patients were initially treated with IAC combined with *** clinical status and complications were recorded at each visit.●RESULTS:The International Intraocular Retinoblastoma Classification groups were D in 23 eyes and E in 7 *** eyes showed severe cloud vitreous seeds at the first *** mean number of IAC cycles and intravitreal injections was 3.2(range,3-4)and 6(range,1-14),*** median follow-up time was 29 mo(range,7-36 mo).Treatment success with regression of the retinal tumor and vitreous seeds was achieved in 29 of 30 eyes(96.7%).Globe salvage was attained in 93.3%(28/30)eyes,and enucleation(n=2)was per formed due to neovascular glaucoma and persistent vitreous *** included retinal pigment epithelium(RPE)atrophy(n=13;43%),mild lens opacity(n=7;23%),vitreous hemorrhage(n=5;17%)and rhegmatogenous retinal detachment(n=1;3%).No extraocular tumor extension or metastasis occurred.●CONCLUSION:Combined IAC and IVM is effective and safe for the treatment of advanced unilateral retinoblastoma.

关键词： intra-arterial chemotherapy intravitreal chemotherapy melphalan retinoblastoma advanced stage unilateral disease

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Tissue plasminogen activator-assisted vitrectomy in the early treatment of acute massive suprachoroidal hemorrhage complicating cataract surgery

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International Journal of Ophthalmology(English edition) 2018年第1期11卷 170-171页

作者： Ping Fei Hai-Ying Jin Qi Zhang Xin Li pei-quan zhao Department of Ophthalmology Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

Dear Editor,I am *** PQ,from the Department of Ophthalmology,Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,China.

关键词： Tissue plasminogen the resistant spasm of accommodation

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Novel in-frame deletion mutation c.177_179del TAC of neurofibromatosis type 1 in a Chinese 4-year-old boy with binocular blindness

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International Journal of Ophthalmology(English edition) 2015年第5期8卷 1078-1079页

作者： Jie Peng Jia Zhang Qi Zhang pei-quan zhao Zhi-Rong Yao Department of Ophthalmology Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Department of Dermatology Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

Dear editor,I am *** Peng,from the Department of Ophthalmology,Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,China.I write to present a case report of a novel in-frame deletion mutation c.17779del TAC of neurofibromatosis type 1 in a Chinese boy with bilateral *** type 1(NF1;OMIM#162200),an autosomal dominant disease,is caused by mutations in the *** incidence of this disease is around 1 in 3500

关键词： Novel in-frame deletion mutation c.177179del TAC of neurofibromatosis type 1 in a Chinese 4-year-old boy with binocular blindness type

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