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Overexpression of Soluble Human Thymosin Alpha 1 in Escherichia coli

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Acta Biochimica et Biophysica Sinica 2005年第2期37卷 147-151页

作者： pei-Fu chen hong-Ying ZHANG Geng-Feng FU Gen-Xing XU Ya-Yi HOU Medical School,Nanjing University.Nanjing 210093,China School of Life Sciences.Nanjing University.Nanjing 210093,China Medical School Nanjing University Nanjing 210093 China School of Life Sciences Nanjing University Nanjing 210093 China

Synthesized gene of human thymosin alpha 1（Tα1）was inserted into pET-28a,pET-9c,pThioHis ***-2T or pBV222 and then inductively expressed in strains of Escherichia *** thefive expression systems,the BL21/pET-28a system provides the highest expression level of fusion protein ina soluble form,which is up to 70% of total expressed bacterial proteins as visualized by sodium dodecylsulfate-polyacrylamide gel electrophoresis（SDS-PAGE）.The resulting fusion protein purified through nickelaffinity chromatography accounts for 2.53% of the wet bacterial pellet weight and reaches 94.5% purity *** results indicate the potential of this expression system for high-throughput production ofrecombinant Tα1.

关键词： human thymosin alpha l Escherichia coli fusion expression Ni2+ affinity chromatography

Depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 335-345页

作者： Feng Zhang chenyang Zhang Linlin Wu Wei Sun hongliang Zhang Jianhua chen Yong pei Songjiang Li Engineering Research Center of Ministry of Education for Carbon Emission Reduction in Metal Resource Exploitation and Utilization Hunan International Joint Research Center for Efficient and Clean Utilization of Critical Metal Mineral ResourcesSchool of Minerals Processing and BioengineeringCentral South UniversityChangsha 410083China State Key Laboratory of Mineral Processing Beijing General Research Institute of Mining&MetallurgyBeijing 100160China Hunan Provincial Key Laboratory of Micro&Nano Materials Interfaces Science College of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083China School of Resources Environment and MaterialsGuangxi UniversityNanning 530004China Department of Chemistry Xiangtan UniversityXiangtan 411105China China Railway Resource Group Co.Ltd Beijing 102300China

The depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite still lacked in-depth ***,the depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite was further systematically investigated with experiments and density functional theory(DFT)*** X-ray photoelectric spectroscopy(XPS)results,DFT calculation results,and frontier molecular orbital analysis indicated that sulfite ions were difficult to be adsorbed on sphalerite surface,suggesting that sulfite ions achieved depression effects on sphalerite through other non-adsorption ***,the oxygen content in the surface of sphalerite treated with sulfite ions in creased,which enhanced the hydrophilicity of the sphalerite and further increased the difference in hydrophilicity between sphalerite and ***,sulfite ions were chelated with lead ions to form PbSO_(3)in *** hydrophilic PbSO_(3)was more easily adsorbed on sphalerite than *** interaction between sulfite ions and lead ions could effectively inhibit the activation of *** addition the UV spectrum showed that after adding sulfite ions,the peak of perxanthate in the sphalerite treated xanthate solution was significantly stronger than that in the galena with xanthate solution,indicating that xanthate interacted more readily with sulfite ions and oxygen mo lecules within the sphalerite system,leading to the formation of ***,sulfite ions hardly depressed the flotation of ga lena and could promote the flotation of galena to some *** study deepened the understanding of the depression mechanism o sulfite ions on sphalerite and Pb^(2+)activated sphalerite.

关键词： sphalerite galena sulfite ion density functional theory depression mechanism

Advances in therapies using mesenchymal stem cells and their exosomes for treatment of peripheral nerve injury:state of the art and future perspectives

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Neural Regeneration Research 2025年第11期20卷 3151-3171页

作者： Fatima Aldali Chunchu Deng Mingbo Nie hong chen Department of Rehabilitation Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Orthopedics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

“Peripheral nerve injury”refers to damage or trauma affecting nerves outside the brain and spinal *** nerve injury results in movements or sensation impairments,and represents a serious public health *** severed peripheral nerves have been effectively joined and various therapies have been offered,recovery of sensory or motor functions remains limited,and efficacious therapies for complete repair of a nerve injury remain *** emerging field of mesenchymal stem cells and their exosome-based therapies hold promise for enhancing nerve regeneration and *** stem cells,as large living cells responsive to the environment,secrete various factors and *** latter are nano-sized extracellular vesicles containing bioactive molecules such as proteins,microRNA,and messenger RNA derived from parent mesenchymal stem *** have pivotal roles in cell-to-cell communication and nervous tissue function,offering solutions to changes associated with cell-based *** ongoing investigations,mesenchymal stem cells and mesenchymal stem cell-derived exosome-based therapies are in the exploratory stage.A comprehensive review of the latest preclinical experiments and clinical trials is essential for deep understanding of therapeutic strategies and for facilitating clinical *** review initially explores current investigations of mesenchymal stem cells and mesenchymal stem cell-derived exosomes in peripheral nerve injury,exploring the underlying ***,it provides an overview of the current status of mesenchymal stem cell and exosomebased therapies in clinical trials,followed by a comparative analysis of therapies utilizing mesenchymal stem cells and ***,the review addresses the limitations and challenges associated with use of mesenchymal stem cell-derived exosomes,offering potential solutions and guiding future directions.

关键词： clinical trials exosome extracellular vesicles mesenchymal stem cells nerve regeneration peripheral nerve injury pre-clinical experiments

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平台型企业生态圈战略研究前沿探析

上海对外经贸大学学报 2017年第5期24卷 54-65页

作者：谢佩洪陈昌东周帆上海对外经贸大学工商管理学院上海201620

平台型企业作为一种新的组织形态在移动互联网时代彰显出巨大的影响力,双边或多边市场主体借助平台能够降低交易成本,并且可以低成本地利用平台提供的各种资源。本文在明确平台、平台型企业以及平台生态圈概念的基础上,对平台的特征和... 详细信息

平台型企业作为一种新的组织形态在移动互联网时代彰显出巨大的影响力,双边或多边市场主体借助平台能够降低交易成本,并且可以低成本地利用平台提供的各种资源。本文在明确平台、平台型企业以及平台生态圈概念的基础上,对平台的特征和分类、平台网络效应、竞争策略、成长过程和平台生态圈的构建等国内外研究进行了全面的梳理和述评。基于平台型企业生态圈对理论研究和企业实践的重要价值,提出了未来值得研究的潜在方向。

关键词：双边市场平台型企业网络效应平台生态圈战略

Study on design and experimental of a piezoelectric linear motor using columnar actuator

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Study on design and experimental of a piezoelectric linear m...

稀土对ZG60CrMnSiMo钢冲击疲劳抗力的影响

2011年全国压电和声波理论及器件应用研讨会

作者： Wei-hua Liu~* Yin Wang pei-hong chen Wei-qing Huang State Key Laboratory of Mechanics and Control of Mechanical Structures, Nanjing University of Aeronautics and Astronautics,Nanjing 210016,China

＜正＞With the rapid development of modern precision manufacturing industry,actuators with high resolution and long stroke are needed in many application *** of the unique advantages such as self-locking on the power ... 详细信息

关键词： piezoelectric linear motor piezoelectric stacks non-resonant vibration stator driving foot experimental study

来源： cnki会议评论

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稀土 1999年第6期20卷 32-35页

作者：林国荣陈东顾泉佩陈鸿玲福州大学机械系福建福州350002

加入适量稀土能提高ＺＧ６０ＣｒＭｎＳｉＭｏ钢的冲击疲劳抗力，过量反而导致性能恶化。

关键词：低合金钢冲击疲劳抗力稀土元素稀土加入量

微小RNA-29b在非小细胞肺癌中的表达及其抑制A549细胞增殖的作用

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中国临床药理学杂志 2018年第14期34卷 1604-1607页

作者：王培马红冰陈栋李勇李晓辉高翔河南大学淮河医院胸心外科河南开封475000

目的研究miR-29b在非小细胞肺癌(NSCLC)组织中的表达及其在肺腺癌细胞A549细胞株中的生物学功能。方法选择2013年6月至2015年5月河南大学淮河医院手术切除的NSCLC患者40例为研究对象,用实时荧光定量PCR聚合酶链反应法检测40例NSCLC患者... 详细信息

目的研究miR-29b在非小细胞肺癌(NSCLC)组织中的表达及其在肺腺癌细胞A549细胞株中的生物学功能。方法选择2013年6月至2015年5月河南大学淮河医院手术切除的NSCLC患者40例为研究对象,用实时荧光定量PCR聚合酶链反应法检测40例NSCLC患者癌组织和癌旁组织石蜡标本中的miR-29b的表达水平并探讨其与患者临床病理学特征的关系。用脂质体法将外源性miR-29b模拟物miR-29b mimics转染至人肺腺癌细胞株A549,用CCK-8实验和Transwell实验评价miR-29b mimics对A549细胞增殖及侵袭能力的影响。结果 40例NSCLC患者癌组织和癌旁组织中miR-29b的表达水平分别为-1.74±1.26,-0.63±0.54,癌组织显著低于癌旁组织,差异有统计学意义(P0.05),但与患者的临床分期有关(P0.05)。结论微小RNA miR-29b在非小细胞肺癌患者癌组织中表达水平显著降低,过表达miR-29b可抑制肺癌A549细胞增殖。

关键词：微小RNA 微小RNA-29b 非小细胞肺癌增殖

Overexpression of low-density lipoprotein receptor prevents neurotoxic polarization of astrocytes via inhibiting NLRP3 inflammasome activation in experimental ischemic stroke

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Neural Regeneration Research 2025年第2期20卷 491-502页

作者： Shuai Feng Juanji Li Tingting Liu Shiqi Huang Xiangliang chen Shen Liu Junshan Zhou hongdong Zhao Ye hong Department of Neurology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina Department of Neurology Nanjing Jinling HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjingJiangsu ProvinceChina

Neurotoxic astrocytes are a promising therapeutic target for the attenuation of cerebral ischemia/reperfusion ***-density lipoprotein receptor,a classic cholesterol regulatory receptor,has been found to inhibit NLR family pyrin domain containing protein 3(NLRP3)inflammasome activation in neurons following ischemic stroke and to suppress the activation of microglia and astrocytes in individuals with Alzheimer’s ***,little is known about the effects of low-density lipoprotein receptor on astrocytic activation in ischemic *** address this issue in the present study,we examined the mechanisms by which low-density lipoprotein receptor regulates astrocytic polarization in ischemic stroke ***,we examined low-density lipoprotein receptor expression in astrocytes via immunofluorescence staining and western blotting *** observed significant downregulation of low-density lipoprotein receptor following middle cerebral artery occlusion reperfusion and oxygen-glucose deprivation/***,we induced the astrocyte-specific overexpression of low-density lipoprotein receptor using astrocyte-specific adeno-associated ***-density lipoprotein receptor overexpression in astrocytes improved neurological outcomes in middle cerebral artery occlusion mice and reversed neurotoxic astrocytes to create a neuroprotective ***,we found that the overexpression of low-density lipoprotein receptor inhibited NLRP3 inflammasome activation in oxygen-glucose deprivation/reoxygenation injured astrocytes and that the addition of nigericin,an NLRP3 agonist,restored the neurotoxic astrocyte *** findings suggest that low-density lipoprotein receptor could inhibit the NLRP3-meidiated neurotoxic polarization of astrocytes and that increasing low-density lipoprotein receptor in astrocytes might represent a novel strategy for treating cerebral ischemic stroke.

关键词： inflammation ischemia/reperfusion injury ischemic stroke low-density lipoprotein receptor neuroprotective astrocytes neurotoxic astrocytes NLRP3 inflammasome polarization

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一起挪威疥疮医院感染暴发的调查与防控

中国感染控制杂志 2017年第8期16卷 749-751页

作者：王培红陈彩林黄茜莎王斌华南京医科大学附属江苏盛泽医院江苏苏州215228

目的了解某院一起挪威疥疮医院感染暴发的流行病学特点,为临床预防和控制医院感染提供依据。方法采用现场流行病学调查方法,对全部可疑感染者和密切接触者进行调查并追踪,描述患者三间分布,制定并落实一系列有效的综合防控措施。结果 2... 详细信息

目的了解某院一起挪威疥疮医院感染暴发的流行病学特点,为临床预防和控制医院感染提供依据。方法采用现场流行病学调查方法,对全部可疑感染者和密切接触者进行调查并追踪,描述患者三间分布,制定并落实一系列有效的综合防控措施。结果 2015年11月3—16日共有16例人员发生挪威疥疮感染,发病例数最多的时间为11月11日(5例),发病时间集中在11月9—11日,共10例,占发病总数的62.50%。16例病例空间分布:综合内科12例、护理部2例、员工家庭2例。人群分布:主要为50~59岁患者(7例)、女性(13例)、护理人员(9例)。采取综合防控措施、使用药物治疗后,16例病例均痊愈,治愈率为100%。病区内和医院内无新发病例出现,疫情得到控制。结论挪威疥疮具有高度接触传染性,可在局部地区造成流行。为避免疥疮在医院内感染蔓延、扩散,医务人员需加强对该病的诊断及防护水平。

关键词：挪威疥疮医院感染暴发防控措施