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Quantitative analysis of serum protein in patients with acute coronary syndrome

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South China Journal of Cardiology 2018年第4期19卷 268-278页

作者： WANG pei-ning ZHANG Bin JIN Li-jun DONG Tai-ming Department of Cardiology Guangdong Provincial Cardiovascular Institute Guangdong General Hospital Guangdong Academy of Medical Sciences

Background The early diagnosis of acute coronary syndromes(ACS)is of great significance for saving patient′s life. The high-resolution and high-throughput proteomic techniques provide a new means for the discovery of disease-specific *** screen serum protein molecules associated with ACS using differential proteomics methods with isotope-labeled relative and absolute quantification(iTRAQ). Method Coronary sinus serum(10 in each group)was collected from ACS patients and healthy controls with the same Genetic background. iTRAQ analysis was performed to screen for significantly different proteins between ACS and healthy controls after pretreatment with high-abundance protein. Result In the iTRAQ analysis of the serum samples from two groups,92 proteins with a mean difference of more than 1.5 times were screened in serum of patients with ACS compared with healthy control serum. The functions of these proteins are involved in stress response,transport,immune response and signal transduction. Conclusions ACS is associated with altered expression of many proteins which may serve as the potential markers of ACS in serum.

关键词： acute coronary syndrome serum sotope-labeled relative and absolute quantification(iTRAQ) proteomics

Outcomes of combined mitochondria and mesenchymal stem cellsderived exosome therapy in rat acute respiratory distress syndrome and sepsis

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World Journal of Stem Cells 2024年第6期16卷 690-707页

作者： Kun-Chen Lin Wen-Feng Fang Jui-ning Yeh John Y Chiang Hsin-Ju Chiang pei-Lin Shao pei-Hsun Sung Hon-Kan Yip Department of Anesthesiology Kaohsiung Chang Gung Memorial HospitalKaohsiung 83301TaiwanChina Division of Pulmonary and Critical Care Medicine Department of Internal MedicineKaohsiung Chang Gung Memorial HospitalChang Gung University College of MedicineKaohsiung 83301TaiwanChina Department of Cardiology The First Affiliated Hospital of Jinan UniversityGuangzhou 510630Guangdong ProvinceChina Department of Computer Science and Engineering National Sun Yat-sen UniversityKaohsiung 804201TaiwanChina Department of Healthcare Administration and Medical Informatics Kaohsiung Medical UniversityKaohsiung 80708TaiwanChina School of Medicine Chung Shan Medical UniversityTaichung 40201TaiwanChina Department of Nursing Asia UniversityTaichung 41354TaiwanChina Division of Cardiology Department of Internal MedicineKaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineKaohsiung 83301TaiwanChina Institute for Translational Research in Biomedicine Kaohsiung Chang Gung Memorial HospitalKaohsiung 83301TaiwanChina Center for Shockwave Medicine and Tissue Engineering Kaohsiung Chang Gung Memorial HospitalKaohsiung 83301TaiwanChina Department of Medical Research China Medical University HospitalChina Medical UniversityTaichung 40402TaiwanChina School of Medicine College of MedicineChang Gung UniversityTaoyuan 33302TaiwanChina

BACKGROUND The treatment of acute respiratory distress syndrome(ARDS)complicated by sepsis syndrome(SS)remains *** To investigate whether combined adipose-derived mesenchymal-stem-cells(ADMSCs)-derived exosome(EXAD)and exogenous mitochondria(mitoEx)protect the lung from ARDS complicated by *** In vitro study,including L2 cells treated with lipopolysaccharide(LPS)and in vivo study including male-adult-SD rats categorized into groups 1(sham-operated-control),2(ARDS-SS),3(ARDS-SS+EXAD),4(ARDS-SS+mitoEx),and 5(ARDS-SS+EXAD+mitoEx),were included in the present *** In vitro study showed an abundance of mitoEx found in recipient-L2 cells,resulting in significantly higher mitochondrial-cytochrome-C,adenosine triphosphate and relative mitochondrial DNA levels(P<0.001).The protein levels of inflammation[interleukin(IL)-1β/tumor necrosis factor(TNF)-α/nuclear factor-κB/toll-like receptor(TLR)-4/matrix-metalloproteinase(MMP)-9/oxidative-stress(NOX-1/NOX-2)/apoptosis(cleaved-caspase3/cleaved-poly(ADP-ribose)polymerase)]were significantly attenuated in lipopolysaccharide(LPS)-treated L2 cells with EXAD treatment than without EXAD treatment,whereas the protein expressions of cellular junctions[occluding/β-catenin/zonula occludens(ZO)-1/E-cadherin]exhibited an opposite pattern of inflam-mation(all P<0.001).Animals were euthanized by 72 h post-48 h-ARDS induction,and lung tissues were *** 72 h,flow cytometric analysis of bronchoalveolar lavage fluid demonstrated that the levels of inflam-matory cells(Ly6G+/CD14+/CD68+/CD11b/c+/myeloperoxidase+)and albumin were lowest in group 1,highest in group 2,and significantly higher in groups 3 and 4 than in group 5(all P<0.0001),whereas arterial oxygen-saturation(SaO2%)displayed an opposite pattern of albumin among the *** findings of lung injury/fibrosis area and inflammatory/DNA-damaged markers(CD68+/γ-H2AX)displayed an identical pattern of SaO2%among the groups(all P<0.0001).The pro

关键词： Acute respiratory distress syndrome Sepsis syndrome Exosomes Mitochondria Adipose tissue-derived mesenchymal stem cells Inflammation

社会心理因素与交通环境对行人分心的联合影响

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中华疾病控制杂志 2023年第2期27卷 224-230页

作者：宁佩珊胡国清中南大学湘雅公共卫生学院流行病与卫生统计学系长沙410078

目的基于联合行为模型检验社会心理因素和交通环境对使用手机、与同行人员交谈、吃喝东西/吸烟3类行人分心的联合影响。方法采用多阶段随机抽样法抽取长沙市20个十字交叉路口作为研究现场,综合使用视频拍摄和现场问卷调查收集行人分心... 详细信息

目的基于联合行为模型检验社会心理因素和交通环境对使用手机、与同行人员交谈、吃喝东西/吸烟3类行人分心的联合影响。方法采用多阶段随机抽样法抽取长沙市20个十字交叉路口作为研究现场,综合使用视频拍摄和现场问卷调查收集行人分心、社会心理因素和交通环境等数据。参照联合行为模型,从行人对不同分心的态度、主观规范、个人能动性和分心习惯等社会心理因素角度设计调查问卷。采用结构方程模型检验社会心理因素和交通环境对行人分心的联合影响。结果研究者共邀请到1974名行人参与问卷调查,其中有效问卷1741份(手机使用问卷600份、与同行人员交谈问卷660份、吃喝东西/吸烟问卷481份)。个人态度、主观规范、个人能动性、意图、分心习惯和交通环境因素共同解释行人手机使用、与同行人员交谈和吃喝/吸烟分心变异的22%、18%和23%。个人能动性、主观规范、个人态度是手机使用分心的主要解释因子,其间接效应分别为0.126、0.110和0.102(均有P<0.05);分心习惯是与同行人员交谈、吃喝东西/吸烟分心的主要解释因子,其总效应分别为0.158和0.250(均有P<0.05)。交通环境对3类行人分心的影响均无统计学意义。结论社会心理因素和交通环境共同影响手机使用、与同行人员交谈和吃喝东西/吸烟3类行人分心的发生,不同分心的主要解释因子存在差异。未来应针对不同行人分心的主要解释因子,综合采用教育、立法和工程干预以减少行人分心。

关键词：行人过街分心社会心理因素交通环境联合行为模型

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王家岭选煤厂全粒级分选工艺

煤炭工程 2018年第6期50卷 95-97页

作者：史培宁中煤西安设计工程有限责任公司陕西西安710054

王家岭选煤厂入洗原煤煤质属于瘦煤(SM),是宝贵的优质炼焦配煤,科学有效的分选工艺是精煤最大回收率和企业经济效益的保证,针对入洗原煤煤质,+50mm粒级块煤动筛跳汰机预排矸工程投资少、吨煤耗水量小于0.1m3,生产成本低;1~50mm粒级重介... 详细信息

王家岭选煤厂入洗原煤煤质属于瘦煤(SM),是宝贵的优质炼焦配煤,科学有效的分选工艺是精煤最大回收率和企业经济效益的保证,针对入洗原煤煤质,+50mm粒级块煤动筛跳汰机预排矸工程投资少、吨煤耗水量小于0.1m3,生产成本低;1~50mm粒级重介旋流器主再洗灵活可靠,吨煤介耗1kg,精煤回收率高;1~0.25mm粒级采用TBS干扰床分选机分选指标Ep值0.18,I值0.24,分选效果好;0.25~0mm粒级微泡浮选柱分选浮选精煤产出率保持70%以上,实践证明,该厂采用全粒级分选工艺,产品煤数质量指标优良,经济效益显著。

关键词：排矸动筛重介主再洗 TBS 微泡浮选柱

塑胶面层材料对SD大鼠的亚慢性吸入染毒研究

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毒理学杂志 2024年第4期38卷 265-269页

作者：李光先梁俊杰黄晓辉李培宁李敏朱素珍钟婉玲刘香梅广州质量监督检测研究院广东广州511447

目的建立塑胶面层材料致大鼠肺损伤的动物模型,为后期毒性机制研究提供基础。方法将检疫合格的SD大鼠按体重随机分为暴露组和对照组,每组20只,雌雄各半。采用吸入暴露方式进行染毒,每天吸入2 h,连续染毒90 d。实验结束后,对大鼠进行剖... 详细信息

目的建立塑胶面层材料致大鼠肺损伤的动物模型,为后期毒性机制研究提供基础。方法将检疫合格的SD大鼠按体重随机分为暴露组和对照组,每组20只,雌雄各半。采用吸入暴露方式进行染毒,每天吸入2 h,连续染毒90 d。实验结束后,对大鼠进行剖检,测量血常规、凝血和血生化相应指标,称量脏器重量、计算脏体比,对脏器进行组织病理学检查。结果染毒期间暴露组雄性大鼠的体重以及体重增量较对照组降低(t=2.436,P<0.05)。染毒结束后,与对照组比较,暴露组雌、雄大鼠肺重量、肺体比和脑体比均上升(t=-3.277,P<0.01;t=-2.865,P<0.05)。脏器组织病理学检查结果显示,与对照组比较,暴露组雌、雄大鼠出现病理改变(间质性肺炎、肺出血和肺水肿)的动物数量明显增多(χ^(2)=5.000,P<0.05)。结论在本实验条件下,根据脏器重量、脏体比以及肺病理改变判定塑胶面层材料致大鼠肺损伤模型成立。

关键词：塑胶面层材料 SD大鼠肺损伤动物模型

Protective Effects of Danmu Extract Syrup on Acute Lung Injury Induced by Lipopolysaccharide in Mice through Endothelial Barrier Repair

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Chinese Journal of Integrative Medicine 2024年第3期30卷 243-250页

作者： XU Han XU Si-cong LI Li-yan WU Yu-huang TAN Yin-feng CHEN Long LIU pei LIANG Chang-fu HE Xiao-ning LI Yong-hui Hainan Provincial Key Lab of Research&Development on Tropic Herbs Hainan Medical UniversityHaikou571199China Department of Stomatology the Second Affiliated Hospital of Hainan Medical UniversityHaikou571199China

Objective:To investigate the effects of Danmu Extract Syrup(DMS) on lipopolysaccharide(LPS)-induced acute lung injury(ALI) in mice and explore the ***:Seventy-two male Balb/C mice were randomly divided into 6 groups according to a random number table(n=12),including control(normal saline),LPS(5 mg/kg),LPS+DMS 2.5 mL/kg,LPS+DMS 5 mL/kg,LPS+DMS 10 mL/kg,and LPS+Dexamethasone(DXM,5 mg/kg) *** pretreatment with DMS and DXM,the ALI mice model was induced by LPS,and the bronchoalveolar lavage fluid(BALF) were collected to determine protein concentration,cell counts and inflammatory *** lung tissues of mice were stained with hematoxylin-eosin,and the wet/dry weight ratio(W/D) of lung tissue was *** levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and IL-1βin BALF of mice were detected by enzyme linked immunosorbent *** expression levels of Claudin-5,vascular endothelial(VE)-cadherin,vascular endothelial growth factor(VEGF),phospho-protein kinase B(p-Akt) and Akt were detected by Western blot ***:DMS pre-treatment significantly ameliorated lung histopathological *** with the LPS group,the W/D ratio and protein contents in BALF were obviously reduced after DMS pretreatment(P<0.05 or P<0.01).The number of cells in BALF and myeloperoxidase(MPO) activity decreased significantly after DMS pretreatment(P<0.05 or P<0.01).DMS pre-treatment decreased the levels of TNF-α,IL-6 and IL-1β(P<0.01).Meanwhile,DMS activated the phosphoinositide 3-kinase/protein kinase B(PI3K/Akt) pathway and reversed the expressions of Claudin-5,VE-cadherin and VEGF(P<0.01).Conclusions:DMS attenuated LPS-induced ALI in mice through repairing endothelial *** might be a potential therapeutic drug for LPS-induced lung injury.

关键词： Danmu Extract Syrup acute lung injury inflammatory factors vascular endothelial growth factor phosphoinositide 3-kinase/protein kinase B Chinese medicine Nauclea officinalis

基于视频拍摄的长沙市十字交叉路口行人过街分心的流行特征分析

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中华疾病控制杂志 2022年第11期26卷 1339-1343页

作者：宁佩珊胡国清中南大学湘雅公共卫生学院流行病与卫生统计学系长沙410078

目的分析长沙市过街行人常见分心类型的流行特征。方法采用多阶段随机抽样获取研究对象,通过视频拍摄和人工转录获得长沙市20个十字交叉路口过街行人的常见分心发生率及其特征(包括:发生频次、分心时长)。采用χ^(2)检验、Cochran-Arm... 详细信息

目的分析长沙市过街行人常见分心类型的流行特征。方法采用多阶段随机抽样获取研究对象,通过视频拍摄和人工转录获得长沙市20个十字交叉路口过街行人的常见分心发生率及其特征(包括:发生频次、分心时长)。采用χ^(2)检验、Cochran-Armitage趋势检验、Kruskal-Wallis H检验进行统计分析。结果共观察到25 436名20岁及以上过街行人,分心发生率为34.32%(95%CI:33.73%~34.90%),女性、20~<40岁、下午和周末出行行人的分心发生率分别高于男性、其他年龄组、上午和工作日出行者(均有P<0.05)。手机使用、与其他行人交谈、吃喝东西/吸烟的发生率分别为11.06%、19.62%和2.13%。三类分心的发生频次均以1~2次为主,与其他行人交谈(10.0 s)的中位分心时长高于手机使用(9.0 s)和吃喝东西/吸烟(8.0 s)。结论长沙市十字交叉路口过街行人分心发生率高,与其他行人交谈和手机使用是最常见的分心类型。不同分心类型的发生频次和分心时长存在差异。

关键词：行人过街分心发生率流行特征

在数字时代培育专家学习者——学习的通用设计

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电化教育研究 2016年第5期37卷 58-65页

作者：陈舒裴新宁华东师范大学课程与教学研究所上海200062

学会学习、成为学习的"自治者"已成为21世纪人才培养的目标。依托数字时代的技术优势,每一位学习者都拥有成为专家学习者的潜在可能。学习的通用设计(UDL)将"以学习者为中心"的理念与"技术改变教育"的理想紧密相连,通过提供三大原则及... 详细信息

学会学习、成为学习的"自治者"已成为21世纪人才培养的目标。依托数字时代的技术优势,每一位学习者都拥有成为专家学习者的潜在可能。学习的通用设计(UDL)将"以学习者为中心"的理念与"技术改变教育"的理想紧密相连,通过提供三大原则及设计指南,为多样的学习者创设丰富的选择,是数字时代培育专家学习者的有益路径。数字化学习环境、网络资源工具等为UDL的实施提供了强大的助推力。

关键词：专家学习者学习的通用设计技术数字时代

教师话语策略与学生科学推理学习的关系——基于全班对话的实证研究

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教师教育研究 2020年第4期32卷 111-121页

作者：赵楠裴新宁华东师范大学教师教育学院上海200062

课堂对话质量关乎学生科学推理的学习效果,已经成为国际科学教育研究界共识。但大量研究聚焦于小组对话对科学推理学习的影响,较少研究涉及课堂对话的主流模式——全班对话。本研究基于学习的社会文化观,选取我国优秀的科学推理教学案例... 详细信息

课堂对话质量关乎学生科学推理的学习效果,已经成为国际科学教育研究界共识。但大量研究聚焦于小组对话对科学推理学习的影响,较少研究涉及课堂对话的主流模式——全班对话。本研究基于学习的社会文化观,选取我国优秀的科学推理教学案例,探讨全班对话场景下教师话语策略与学生科学推理学习之间的关系。研究发现,教师话语策略与学生语言科学性之间存在显著关联,且教师要求学生解释的话语策略对学生科学推理起主要的推动作用。研究有助于唤起我国探究式课堂对教师话语质量的关注,以提升学生科学推理学习的成效。

关键词：科学推理师生对话全班讨论话语分析