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Corneal collagen crosslinking in keratoconus and other eye disease

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International Journal of Ophthalmology(English edition) 2015年第2期8卷 407-418页

作者： Adel Alhayek pei-Rong lu Department of Ophthalmology the First Affiliated Hospital of Soochow University

Keratoconus is a condition characterized by biomechanical instability of the cornea, presenting in a progressive, asymmetric and bilateral way. Corneal collagen crosslinking(CXL) with riboflavin and Ultraviolet-A(UVA) is a new technique of corneal tissue strengthening that combines the use of riboflavin as a photo sensitizer and UVA irradiation. Studies showed that CXL was effective in halting the progression of keratoconus over a period of up to four years. The published studies also revealed a reduction of max K readings by more than 2 D, while the postoperative spherical equivalent(SEQ) was reduced by an average of more than 1 D and refractive cylinder decreased by about1 D. The major indication for the use of CXL is to inhibit the progression of corneal ecstasies, such as keratoconus and pellucid marginal degeneration. CXL may also be effective in the treatment and prophylaxis of iatrogenic keratectasia, resulting from excessively aggressive photo ablation. This treatment has been used to treat infectious corneal ulcers with apparent favorable results. Most recent studies demonstrate the beneficial impact of CXL for iatrogenic ecstasies, pellucid marginal degeneration, infectious keratitis, bullous keratopathy and ulcerative keratitis. Several long-term and short-term complications of CXL have been studied and documented. The possibility of a secondary infection after the procedure exists because the patient is subject to epithelial debridement and the application of a soft contact lens. Formation of temporary corneal haze,permanent scars, endothelial damage, treatment failure,sterile infiltrates, bullous keratopathy and herpes reactivation are the other reported complications of this procedure.

关键词： keratoconus collagen corneal cross-linking ultraviolet radiation and riboflavin

Subcutaneous pedicled propeller flap technique for microscopic reconstruction of eyelid defects

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International Journal of Ophthalmology(English edition) 2023年第7期16卷 1047-1052页

作者： Lei Zhong Qian-Yi lu pei-Rong lu The First Affiliated Hospital of Soochow University Suzhou 215006Jiangsu ProvinceChina

AIM:To describe the subcutaneous pedicled propeller flap technique for the microscopic reconstruction of eyelid defects and evaluate its ***:The clinical data of 23 patients(23 eyes)who underwent microscopic reconstruction of eyelid defects with the subcutaneous pedicled propeller flap technique were retrospectively *** patients underwent eyelid tumor resection and one-stage microscopic reconstruction with the subcutaneous pedicled propeller flap for anterioror posterior-layer eyelid *** survival rate of the propeller flap,eyelid function and appearance,tumor recurrence rate,and patient satisfaction were evaluated after the ***:The patients consisted of 12 men and 11 women,aged 31–82y(mean,58.9y).The longest followup time was 5y,and the shortest was *** the propeller flaps survived *** was no significant difference in color and luster between the flap and adjacent tissues,and there was no dog ear *** obvious scarring was *** were no obvious abnormalities of eyelid morphology or function,and no adverse complications such as exposure keratitis,entropion,ectropion,ptosis,and eyelid *** tumor recurrence was found at the time of the last *** patients were satisfied with the surgical ***:The subcutaneous pedicled propeller flap technique for the microscopic reconstruction of eyelid defects has satisfactor y outcomes in terms of eyelid function and esthetics,and merits clinical application.

关键词： propeller flap eyelid defect microscopic reconstruction

Si@Cu@Au AFM tips for tip-enhanced Raman spectrum

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Science China Chemistry 2015年第9期58卷 1494-1500页

作者： pei lu Jing Li Dong Wang Li-Jun Wan CAS Key Laboratory of Molecular Nanostructures and Nanotechnology Institute of Chemistry Chinese Academy of Sciences Beijing National Laboratory for Molecular Sciences

Tip-enhanced Raman spectrum(TERS) is a scanning probe technique for acquiring chemical information at high spatial resolution and with high chemical sensitivity. The sensitivity of TERS with atomic force microscopy(AFM) system is mainly determined by the metalized tips. Here, we report a fabrication protocol for AFM-TERS tips that incorporate a copper(Cu) primer film between a gold(Au) layer and a Si AFM tip. They were fabricated by coating the Si tip with a 2 nm Cu layer prior to adding a 20 nm Au layer. For top illumination TERS experiments, these tips exhibited superior TERS performance relative to that observed for tips coated with Au only. Samples included graphene, thiophenol and brilliant cresyl blue. The results may derive from the surface roughness of the tip apex and a Cu/Au synergism of local surface plasmon resonances.

关键词： tip enhanced Raman spectrum metalized tip graphene thiophenol brilliant crystal blue

DNA damaging effects of carbofuran and its main metabolites on mice by micronucleus test and single cell gel electrophoresis

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Science China(Life Sciences) 2005年第Z1期48卷 40-47页

作者： ZHOU pei LIU Baofeng lu Yitong Department of Resource and Environment Science School of Agriculture and Biology Shanghai Jiaotong University Shanghai 201101China Department of Resource and Environment Science School of Agriculture and Biology Shanghai Jiaotong University Shanghai 201101China Department of Resource and Environment Science School of Agriculture and Biology Shanghai Jiaotong University Shanghai 201101China

The DNA damaging effects of the carbamate pesticide carbofuran and its four metabolites (carbofuranphenol, 3-ketocarbofuran, 3-hydrocarbofuran and nitrosocarbofuran) on mice were evaluated by single cell gel electrophoresis (SCGE) assay and micronucleus test. KM mice were exposed to test compounds with different doses of 0.1, 0.2 and 0.4mg/kg through intraperitoneal injection two times with an internal of 24 h, and then killed by cervical dislocation 6 h after the second injection. In SCGE assay, isolated mice peripheral blood lymphocytes were employed to determine DNA damaging degree after a 1 h treatment by test compounds and a following electrophoresis. Carbofuran and carbofuranphenol showed negative results in both test and had no obvious toxicity. 3-hydrocarbofuran and nitrosocarbofuran were positive.3-ketocarbofuran could not induce micronucleus formation but caused significant DNA migration in SCGE test. These tests revealed that 3-ketocarbofuran, 3-hydrocarbofuran and nitrosocarbofuran are potential mutagesis and further research is needed.

关键词： carbofuran metabolite, micronucleus, SCGE, DNA damage.

Inhibitory effect of CCR3 signal on alkali-induced corneal neovascularization

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International Journal of Ophthalmology(English edition) 2012年第3期5卷 251-257页

作者： Wen-Juan Zhou, pei-Rong lu Department of Ophthalmology the First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China Jiangsu Institute of Clinic Immunology the First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China

AIM: To investigate the effect of CC chemokine receptor 3 (CCR3) signal on corneal neovascularization (CRNV) induced by alkali burn and to explore its mechanism. METHODS: Specific pathogen-free male BALB/C mice (aged 6-8 weeks) were randomly divided into CCR3-antagonist treated group (experimental group) and control group. CRNV was induced by alkali burn in mice. The time kinetic CCR3 expression in injured corneas was examined by reverse transcription polymerase chain reaction (RT-PCR). CCR3-antagonist (SB-328437 at different concentration of 125 mu g/mL, 250 mu g/mL, and 500 mu g/mL) was locally administrated after alkali injury. The formation of CRNV was assessed by CD31 corneal whole mount staining at two weeks after injury. Monocyte chemotactic protein 1 (MCP-1), monocyte chemotactic protein 3 (MCP-3) expressions in the early phase after injury were quantified and compared by RT-PCR. Macrophage intracorneal accumulation in the early phase after injury was evaluated and compared by immunohistochemistry. RESULTS: Alkali injury induced the time kinetic intracorneal CCR3 expression. 500 mu g/mL of CCR3-antagonist treatment in the early phase but not the late phase resulted in significant impaired CRNV as compared to control group (P luSION: CCR3 signal is involved in alkali-induced CRNV. CCR3-antagonist can inhibit alkali-induced CRNV by reducing the intracorneal MCP-1 and MCP-3 mRNA expression and the intracorneal macrophage infiltration.

关键词： corneal neovascularization CCR3 monocyte chemotactic protein 1 monocyte chemotactic protein 3 macrophage

Retinal ganglion cell-inner plexiform and nerve fiber layers in neuromyelitis optica

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International Journal of Ophthalmology(English edition) 2018年第1期11卷 89-93页

作者： Sai-Jing Hu pei-Rong lu Department of Ophthalmology the First Affiliated Hospital of Soochow University Department of Ophthalmology the First Affiliated Hospital of Wenzhou Medical University

AIM： To determine the thickness of the retinal ganglion cell-inner plexiform layer（GCIPL） and the retinal nerve fiber layer（RNFL） in patients with neuromyelitis optica（NMO）.METHODS： We conducted a cross-sectional study that included 30 NMO patients with a total of 60 eyes. Based on the presence or absence of optic neuritis（ON）, subjects were divided into either the NMO-ON group（30 eyes） or the NMO-ON contra group（10 eyes）. A detailed ophthalmologic examination was performed for each group; subsequently, the GCIPL and the RNFL were measured using highdefinition optical coherence tomography（OCT）. RESULTS： In the NMO-ON group, the mean GCIPL thickness was 69.28±21.12 μm, the minimum GCIPL thickness was 66.02±10.02 μm, and the RNFL thickness were 109.33±11.23, 110.47±3.10, 64.92±12.71 and 71.21±50.22 μm in the superior, inferior, temporal and nasal quadrants, respectively. In the NMO-ON contra group, the mean GCIPL thickness was 85.12±17.09 μm, the minimum GCIPL thickness was 25.39±25.1 μm, and the RNFL thicknesses were 148.33±23.22, 126.36±23.45, 82.21±22.30 and 83.36±31.28 μm in the superior, inferior, temporal and nasal quadrants, respectively. In the control group, the mean GCIPL thickness was 86.98±22.37 μm, the minimum GCIPL thickness was 85.28±10.75 μm, and the RNFL thicknesses were 150.22±22.73, 154.79±60.23, 82.33±7.01 and 85.62±13.81 μm in the superior, inferior, temporal and nasal quadrants, respectively. The GCIPL and RNFL were thinner in the NMO-ON contra group than in the control group（P〈0.05）; additionally, the RNFL was thinner in the inferior quadrant in the NMO-ON group than in the control group（P〈0.05）. Significant correlations were observed between the GCIPL and RNFL thickness measurements as well as between thickness measurements and the two visual field parameters of mean deviation（MD） and corrected pattern standard deviation（PSD） in the NMO-ON group（P〈0.05）. CONCluSION： The thickness of the GCIPL and RNFL, as measured using OCT, may indicate optic

关键词： neuromyelitis optica retinal nerve fiber layer thickness ganglion cell-inner plexiform layer optical coherence tomography

High glucose: activating autophagy and affecting the biological behavior of human lens epithelial cells

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International Journal of Ophthalmology(English edition) 2019年第7期12卷 1061-1066页

作者： Dan Li Gao-Qin Liu pei-Rong lu Department of Ophthalmology the First Affiliated Hospital of Soochow University

AIM: To clarify the effect of autophagy on human lens epithelial cells (HLECs) under high glucose ***: HLECs were cultured with different concentrations of glucose and 3-methyladenine (3-MA);the expression of autophagy-related protein LC3B was detected by Western blotting and immunofluorescence histochemistry. The migration of HLECs was quantified by scratch wound assay and the expression of transforming growth factor-β(TGF-β) was measured by real-time polymerase chain ***: Compared with 5 mmol/L normal glucose treatment, 40 mmol/L glucose treatment can significantly increase the gen eration of autophagosome in HLECs, which could be inhibited by 0.375 mmol/L 3-MA treatment. The migration of HLECs and the expression of TGF-β in HLECs induced by high glucose were significantly suppressed by 0.375 mmol/L 3-MA ***: Autophagy promotes HLECs cell migration and increases the expression of TGF-β after exposed to high glucose, which may relate to the development of diabetic cataract.

关键词： high glucose autophagy lens epithelial cells

Potential involvement of nitric oxide synthase but not inducible nitric oxide synthase in the development of experimental corneal neovascularization

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International Journal of Ophthalmology(English edition) 2011年第4期4卷 343-348页

作者： Yuan Chen Gao-Qin Liu and pei-Rong lu Department of Ophthalmology the First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China

AIM: To investigate the effect of nitric oxide and its synthetase on experimental corneal neovascularization (CRNV). METHODS: CRNV was induced by alkali injury in mice, nitric oxide synthetase (NOS) was inhibited by NG-nitro-L-arginine (L-NAME) and inducible nitric oxide synthetase (iNOS) was inhibited by aminoguanidine hemisulfate salt (AG). The inhibitory effect was detected at day 2 and 4 after corneal alkali injury by reverse transcription polymerase chain reaction (RT-PCR). CRNV was compared between the control and the treated mice by microscopic observation and corneal whole mount CD31 immunostaining. RESULTS: The inhibition of L-NAME to NOS and AG to iNOS after corneal injury was confirmed by RT-PCR (P 0.05). CONCluSION: Our findings suggest that NOS but not iNOS plays a critical role in alkali injury induced CRNV.

关键词： corneal neovascularization nitric oxide synthase inducible nitric oxide synthase

BioLeT: A new design strategy for functional bioluminogenic probes

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Chinese Chemical Letters 2015年第8期26卷 919-921页

作者： Tian-Chao Zhang lu-pei Du Min-Yong Li Department of Medicinal Chemistry Key Laboratory of Chemical Biology (MOE) School of Pharmacy Shandong University

By integrating photoinduced electron transfer（PET） into the design of functional bioluminogenic probes,Urano and his coworkers recently developed a new rational design strategy, BioLeT. It is expected that this BioLeT strategy will enable us to design and develop new bioluminescence probes for detecting various biomolecules with no catalytic or reactive activity.

关键词： probes detecting enable integrating rational utilized challenging instead absolute moiety