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Different responses of soil fauna gut and plant rhizosphere microbiomes to manure applications

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Soil Ecology Letters 2024年第2期6卷 57-69页

作者： Shuai Du Xiang Zhao Yue Zhang Changlong Shu ju-pei Shen Key Laboratory of Urban Environment and Health Ningbo Urban Environment Observation and Research StationInstitute of Urban EnvironmentChinese Academy of SciencesXiamen361021China School of Geographical Sciences Fujian Normal UniversityFuzhou350007China Institute of Plant Nutrition Resources and EnvironmentBeijing Academy of Agriculture and Forestry SciencesBeijing100097China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing100193China Hebei Key Laboratory of Soil Entomology Cangzhou Academy of Agricultural and Forestry SciencesCangzhou061001China

Microbial attributes were compared between soil fauna gut and plant rhizosphere.•Manure applications decreased or increased gut or rhizosphere bacterial diversity.•Stochastic or deterministic processes drove gut or rhizosphere bacterial assembly.•Manure applications increased bacterial network complexity of gut and *** microbes inhabit animals and plants,helping their hosts perform multiple functions in agricultural ***,the responses of soil fauna gut and plant rhizosphere microbiomes to livestock manure applications are still not well *** we fed Protaetia brevitarsis larvae(PBL)with chicken manure and collected their *** frass and manure were applied as fertilizers to lettuce *** then compared the changes of microbial diversity,community assembly,and potential functions between the gut group(i.e.,all PBL gut and frass samples)and the rhizosphere group(i.e.,all rhizosphere soil samples).We revealed that manure applications(i.e.,feeding or fertilization)decreased bacterial diversity in the gut group but increased that in the rhizosphere ***,the proportions of Bacilli in the gut group and Gammaproteobacteria in the rhizosphere group were increased(up to a maximum of 33.8%and 20.4%,respectively)after manure *** and deterministic processes dominated community assembly in the gut and rhizosphere microbiomes,*** applications increased the microbial co-occurrence network complexity of both the gut and rhizosphere ***,the proportions of functional taxa associated with human/animal pathogens in the gut group and carbon/nitrogen cycling in the rhizosphere group were enhanced(up to 2.6-fold and 24.6-fold,respectively).Our findings illustrate the different responses of microbial diversity,community assembly,and potential functions in soil fauna gut and plant rhizosphere to manure *** results could enhance our knowledge on the reasonable utilization of

关键词： soil fauna gut plant rhizosphere manure applications community assembly microbial functions

Outcomes of combined mitochondria and mesenchymal stem cellsderived exosome therapy in rat acute respiratory distress syndrome and sepsis

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World Journal of Stem Cells 2024年第6期16卷 690-707页

作者： Kun-Chen Lin Wen-Feng Fang jui-Ning Yeh John Y Chiang Hsin-ju Chiang pei-Lin Shao pei-Hsun Sung Hon-Kan Yip Department of Anesthesiology Kaohsiung Chang Gung Memorial HospitalKaohsiung 83301TaiwanChina Division of Pulmonary and Critical Care Medicine Department of Internal MedicineKaohsiung Chang Gung Memorial HospitalChang Gung University College of MedicineKaohsiung 83301TaiwanChina Department of Cardiology The First Affiliated Hospital of Jinan UniversityGuangzhou 510630Guangdong ProvinceChina Department of Computer Science and Engineering National Sun Yat-sen UniversityKaohsiung 804201TaiwanChina Department of Healthcare Administration and Medical Informatics Kaohsiung Medical UniversityKaohsiung 80708TaiwanChina School of Medicine Chung Shan Medical UniversityTaichung 40201TaiwanChina Department of Nursing Asia UniversityTaichung 41354TaiwanChina Division of Cardiology Department of Internal MedicineKaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineKaohsiung 83301TaiwanChina Institute for Translational Research in Biomedicine Kaohsiung Chang Gung Memorial HospitalKaohsiung 83301TaiwanChina Center for Shockwave Medicine and Tissue Engineering Kaohsiung Chang Gung Memorial HospitalKaohsiung 83301TaiwanChina Department of Medical Research China Medical University HospitalChina Medical UniversityTaichung 40402TaiwanChina School of Medicine College of MedicineChang Gung UniversityTaoyuan 33302TaiwanChina

BACKGROUND The treatment of acute respiratory distress syndrome(ARDS)complicated by sepsis syndrome(SS)remains *** To investigate whether combined adipose-derived mesenchymal-stem-cells(ADMSCs)-derived exosome(EXAD)and exogenous mitochondria(mitoEx)protect the lung from ARDS complicated by *** In vitro study,including L2 cells treated with lipopolysaccharide(LPS)and in vivo study including male-adult-SD rats categorized into groups 1(sham-operated-control),2(ARDS-SS),3(ARDS-SS+EXAD),4(ARDS-SS+mitoEx),and 5(ARDS-SS+EXAD+mitoEx),were included in the present *** In vitro study showed an abundance of mitoEx found in recipient-L2 cells,resulting in significantly higher mitochondrial-cytochrome-C,adenosine triphosphate and relative mitochondrial DNA levels(Pjunctions[occluding/β-catenin/zonula occludens(ZO)-1/E-cadherin]exhibited an opposite pattern of inflam-mation(all Pjury/fibrosis area and inflammatory/DNA-damaged markers(CD68+/γ-H2AX)displayed an identical pattern of SaO2%among the groups(all P<0.0001).The pro

关键词： Acute respiratory distress syndrome Sepsis syndrome Exosomes Mitochondria Adipose tissue-derived mesenchymal stem cells Inflammation

orecast of peak infection and estimate of excess deaths in COVID-19 transmission and prevalence in Taiyuan City,2022 to 2023

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Infectious Disease Modelling 2024年第1期9卷 56-69页

作者： Jia-Lin Wang Xin-Long Xiao Fen-Fen Zhang Xin pei Ming-Tao Li ju-Ping Zhang juan Zhang Gui-Quan Sun Complex Systems Research Center Shanxi UniversityTaiyuan030006China School of Mathematical Sciences Shanxi UniversityTaiyuan030006China School of Mathematics North University of ChinaTaiyuan030051China College of Mathematics Taiyuan University of TechnologyTaiyuan030024China Complex Systems and Data Science Key Laboratory of Ministry of Education Taiyuan030006China

In this paper,with the method of epidemic dynamics,we assess the spread and prevalence of COVID-19 after the policy adjustment of prevention and control measure in December 2022 in Taiyuan City in China,and estimate the excess population deaths caused by *** on the transmission mechanism of COVID-19 among individuals,a dynamic model with heterogeneous contacts is established to describe the change of control measures and the population's social behavior in Taiyuan *** model is verified and simulated by basing on reported case data from November 8th to December 5th,2022 in Taiyuan city and the statistical data of the questionnaire survey from December 1st to 23rd,2022 in Neijiang *** with reported numbers of permanent residents and deaths from 2017 to 2021 in Taiyuan city,we apply the dynamic model to estimate theoretical population of 2022 under the assumption that there is no effect of *** addition,we carry out sensitivity analysis to determine the propagation character of the Omicron strain and the effect of the control *** a result of the study,it is concluded that after adjusting the epidemic policy on December 6th,2022,three peaks of infection in Taiyuan are estimated to be from December 22nd to 31st,2022,from May 10th to june 1st,2023,and from September 5th to October 13th,2023,and the corresponding daily peaks of new cases can reach 400000,44000 and 22000,*** the end of 2022,excess deaths can range from 887 to 4887,and excess mortality rate can range from 3.06%to 14.82%.The threshold of the infectivity of the COVID-19 variant is estimated 0.0353,that is if the strain infectivity is above it,the epidemic cannot be control with the previous normalization measures.

关键词： Taiyuan city COVID-19 Dynamic model Epidemic scale Epidemic peak Excess death

衡先培辨治垂体瘤术后持续性高钠血症临床经验

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中国中西医结合杂志 2024年第5期44卷 619-622页

作者：全鹏举衡先培福建中医药大学附属人民医院内分泌代谢病科福州350004

位于鞍区的下丘脑—垂体是人体水钠调节中枢。垂体瘤手术损伤导致该中枢受损,进而水钠失衡,发生高钠血症^([1])。多数鞍区术后渴感正常的高钠血症患者可在1周内恢复^([2]),属于短暂性高钠血症。但是,有部分鞍区术后患者高钠血症持续两... 详细信息

位于鞍区的下丘脑—垂体是人体水钠调节中枢。垂体瘤手术损伤导致该中枢受损,进而水钠失衡,发生高钠血症^([1])。多数鞍区术后渴感正常的高钠血症患者可在1周内恢复^([2]),属于短暂性高钠血症。但是,有部分鞍区术后患者高钠血症持续两周以上,常可造成严重后果^([3])。这种持续性高钠血症又分为两种情况:一种去氨加压素有反应;另一种对去氨加压素无明显反应,仅能采取限盐、强制补液来治疗。目前现代医学对第一种情况研究相对较多,有许多案例报道,且相应的评估、治疗均有文献详细记录,医师治疗有据可循^([4,5])。

关键词：高钠血症去氨加压素垂体瘤手术案例报道现代医学衡先培持续性有据可循

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改性聚乙烯醇协同硅酸盐水泥制高强防水石膏

应用化学 2024年第8期41卷 1131-1145页

作者：徐海祥夏举佩昆明理工大学公共安全与应急管理学院昆明650500 昆明理工大学化学工程学院昆明650500 湖北江汉建筑工程机械有限公司荆州434000

以脱硫石膏为基料,以液碱活化聚甲基氢硅氧烷(PMHS)催化改性聚乙烯醇(PVA)防水剂协同硅酸盐水泥研发高强防水石膏。探究了改性PVA防水剂、硅酸盐水泥对脱硫石膏标准试块表面吸水率、力学性能和软化系数的影响;通过红外光谱确定改性疏水... 详细信息

以脱硫石膏为基料,以液碱活化聚甲基氢硅氧烷(PMHS)催化改性聚乙烯醇(PVA)防水剂协同硅酸盐水泥研发高强防水石膏。探究了改性PVA防水剂、硅酸盐水泥对脱硫石膏标准试块表面吸水率、力学性能和软化系数的影响;通过红外光谱确定改性疏水剂、硅酸盐水泥单独及协同研发强防水石膏的连接形式;通过热重仪分析不同掺加剂对石膏热稳定性的影响;研究表明,改性PVA防水剂协同硅酸盐水泥制高强防水石膏的最佳配比为:防水剂120 g、硅酸盐水泥150 g,此时表面吸水率9.35%,绝干抗压强度22.54 MPa、软化系数67.57%,同时有效提高了纸面石膏板的热稳定性。

关键词：液碱活化甲基含氢硅油催化改性改性PVA疏水剂热稳定性高强防水石膏

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叶面调理剂对复合污染农田小麦籽粒Cd、As和Pb累积的阻控效应

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环境科学 2024年第3期45卷 1812-1820页

作者：肖冰王秋实高培培赵全利杨威王钊刘文菊薛培英河北农业大学资源与环境科学学院省部共建华北作物改良与调控国家重点实验室河北省农田生态环境重点实验室保定071000 河北农业大学教学试验农场保定071000 河北民得富生物技术有限公司保定072450 泰安市生态环境局肥城分局泰安271699

针对麦田土壤镉(Cd)、砷(As)和铅(Pb)复合污染问题,采用田间小区试验,于拔节期、孕穗期和灌浆初期对小麦进行叶面单独和混合喷施Zn(0.2%ZnSO_(4))、Mg(0.4%MgSO_(4))和Mn(0.2%MnSO_(4))处理,以期探究叶面调理剂对小麦籽粒Cd、As和Pb累... 详细信息

针对麦田土壤镉(Cd)、砷(As)和铅(Pb)复合污染问题,采用田间小区试验,于拔节期、孕穗期和灌浆初期对小麦进行叶面单独和混合喷施Zn(0.2%ZnSO_(4))、Mg(0.4%MgSO_(4))和Mn(0.2%MnSO_(4))处理,以期探究叶面调理剂对小麦籽粒Cd、As和Pb累积的阻控效应,最终筛选出适用于北方重金属复合污染区有效阻控小麦籽粒中Cd、As和Pb累积的叶面调理剂.结果表明,与对照相比,叶面喷施Zn+Mg+Mn复配溶液能够显著降低小麦籽粒中Cd、As和Pb含量,降幅分别为18.96%、23.87%和51.31%,TF籽粒/秸秆分别降低了14.62%、27.73%和47.70%,该叶面调理剂能够通过抑制Cd、As和Pb从小麦茎叶到籽粒的转运进而降低其在籽粒的累积;研究还发现,对照的籽粒中Cd和As主要分布在中心胚乳(34.08%~37.08%),而Pb主要分布在果皮和种皮(27.78%).喷施Zn+Mg+Mn复配溶液可显著降低Cd和As在糊粉层含量,分别比CK降低81.10%和82.24%,而Pb除果皮和种皮、中心胚乳外,各层次Pb含量均显著降低(降幅为42.85%~91.15%),各层次面粉只有糊粉层(P2)重金属含量与Zn含量呈显著负相关.综上所述,对于北方石灰性土壤Cd、As和Pb中度复合污染农田,于小麦拔节期、孕穗期和灌浆初期各喷施一次Zn+Mg+Mn复配的叶面调理剂,可有效阻控Cd、As和Pb在小麦籽粒尤其是糊粉层的累积.在此基础上,可通过去除麦麸来进一步降低重金属尤其是Cd的污染,保证小麦安全生产和粮食安全.

关键词：小麦重金属复合污染镉砷铅叶面调理剂阻控

含高比例光伏配电网中计及需求侧响应的混合储能配置优化

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高电压技术 2024年第4期50卷 1416-1425页

作者：马丽李伟裴玮肖浩鞠力顾靖达中国科学院电工研究所北京100190 长时规模储能重点实验室(中国科学院) 北京100190 国网北京市电力公司北京100031 北京电力经济技术研究院有限公司北京100055

大力发展光伏等可再生能源是我国实现“双碳”目标的重要途径,而光伏高比例接入配电网后随之带来一系列问题。以含高比例光伏配电网为研究对象,提出了计及用户侧需求响应的混合储能优化配置方法。针对负荷和光伏出力的双重不确定性,提... 详细信息

大力发展光伏等可再生能源是我国实现“双碳”目标的重要途径,而光伏高比例接入配电网后随之带来一系列问题。以含高比例光伏配电网为研究对象,提出了计及用户侧需求响应的混合储能优化配置方法。针对负荷和光伏出力的双重不确定性,提出了基于预测误差概率分布的不确定性场景确定方法。考虑系统不确定性,统筹运行阶段用户侧需求响应调控措施以及潮流约束,提出了储能配置的3层优化模型。针对优化模型提出了相应的分解方法,以及基于列和约束生成算法的求解实施流程。通过在IEEE-33节点测试系统中的应用,使得系统可平移负荷峰值下降了10%,节点电压维持在合理范围,并在指导储能配置等方面具备显著的应用效果。通过分析光伏比例灵敏性可知,不含光伏情况下的储能总容量相比100%光伏比例场景增加了90%。

关键词：储能配置优化光伏配电网需求侧响应系统不确定性

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Effectiveness and Safety of Qishen Yiqi Dripping Pill in Patients with Acute Coronary Syndrome after Percutaneous Coronary Intervention:3-Year Results from a Multicentre Cohort Study

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Chinese Journal of Integrative Medicine 2024年第10期30卷 877-885页

作者： BAI Rui-na GU Feng CHE Qian-zi ZHANG Xuan CAI Ya-jie XI Rui-xi ZHAO Yang GUO Ming DONG Guo-ju GAO Zhu-ye FU Chang-geng WANG pei-li DU Jian-peng ZHANG Da-wu DUAN Wen-hui LI Li-zhi YANG Qiao-ning SHI Da-zhuo Department of Cardiovascular Xiyuan Hospital China Academy of Chinese Medical Sciences National Clinical Research Center for Chinese Medicine Cardiology Department of Evidence-Based Medicine Basic Research Laboratory Institute of Basic Research in Clinical Medicine China Academy of Chinese Medical Sciences Department of Traditional Chinese Medicine Beijing Shangdi Hospital National Medical Products Administration Key Laboratory for Clinical Research and Evaluation of Traditional Chinese Medicine

Objectives: To evaluate the effectiveness and safety of Qishen Yiqi Dripping Pill(QSYQ) in patients with acute coronary syndrome(ACS) after percutaneous coronary intervention(PCI). Methods: This multicentre prospective cohort study was conducted at 40 centers in China. Patients with ACS after PCI entered either the QSYQ or Western medicine(WM) groups naturally based on whether they had received QSYQ before enrollment. QSYQ group received QSYQ(0.52 g, 3 times a day for 12 months) in addition to WM. The primary endpoint included cardiac death, non-fatal myocardial infarction, and urgent revascularization. The secondary endpoint included rehospitalization due to ACS, heart failure, stroke, and other thrombotic events. Quality of life was assessed by the Seattle Angina Questionnaire(SAQ). Results: A total of 936 patients completed follow-up of the primary endpoint from February 2012 to December 2018. Overall, 487 patients received QSYQ and WM. During a median follow-up of 566 days(inter quartile range, IQR, 517–602), the primary endpoint occurred in 46(9.45%) and 65(14.48%) patients in QSYQ and WM groups respectively [adjusted hazard ratio(HR) 0.60, 95% confidence interval(CI) 0.41–0.90; P=0.013]. The secondary endpoint occurred in 61(12.53%) and 74(16.48%) patients in QSYQ and WM groups, respectively(adjusted HR 0.76, 95% CI 0.53–1.09; P=0.136). In sensitivity analysis, the results still demonstrated that WM combined with QSYQ reduced the risk of the primary endpoint(HR 0.67, 95% CI 0.46–0.98; P=0.039). Moreover, QSYQ improved the disease perception domain of the SAQ(P<0.05). Conclusions: In patients with ACS after PCI, QSYQ combined with WM reduced the incidence of the primary endpoint. These findings provide a promising option for managing ACS after PCI and suggest the potential treatment for reducing the risk of primary endpoint included cardiac death, non-fatal myocardial infarction, and urgent revascularization through intermittent administration of QSYQ.(Registra

关键词： Qishen Yiqi Dripping Pill acute coronary syndrome percutaneous coronary intervention primary and secondary endpoint

贝母素甲改善脂多糖联合烟雾诱导小鼠慢性阻塞性肺疾病的机制

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解剖学报 2024年第2期55卷 215-221页

作者：陈培陈小菊杜竺蔓汪操会成都大学附属医院呼吸与危重症医学科成都610081 成都市金牛区营门口社区卫生服务中心全科医学科成都610031

目的探讨贝母素甲(PME)对慢性阻塞性肺疾病(COPD)小鼠的作用和相关机制。方法将80只小鼠随机分为4组(每组20只):对照组、PME组、COPD组和治疗组。使用脂多糖联合烟雾诱导小鼠COPD动物模型。通过组织病理学、超微结构、小鼠肺组织湿/干... 详细信息

目的探讨贝母素甲(PME)对慢性阻塞性肺疾病(COPD)小鼠的作用和相关机制。方法将80只小鼠随机分为4组(每组20只):对照组、PME组、COPD组和治疗组。使用脂多糖联合烟雾诱导小鼠COPD动物模型。通过组织病理学、超微结构、小鼠肺组织湿/干重比值分析PME对COPD模型鼠结构的影响;ELISA和Western blotting分析PME对肺组织中炎症因子肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6和IL-1β表达的影响;二氢乙锭(DHE)染色和Western blotting分析PME对肺组织中氧化应激反应的影响;Western blotting分析PME对核因子κB(NF-κB)通路以及核因子2相关因子2(Nrf2)通路相关蛋白表达的影响。结果与COPD组相比,PME治疗可明显减轻小鼠肺组织的损伤和减少炎症细胞数量,降低肺组织湿/干重比。与对照组相比,COPD组小鼠的肺泡灌洗液中TNF-α、IL-6及IL-1β水平明显增加,经PME治疗后小鼠肺泡灌洗液中TNF-α、IL-6及IL-1β水平明显降低。另外,与对照组相比,COPD组小鼠肺组织中TNF-α和IL-1β水平显著升高,经PME治疗后小鼠肺组织中TNF-α和IL-1β蛋白水平明显下降。免疫组织化学和Western blotting实验显示,与对照组相比,COPD组SOD2水平明显降低,而PME治疗后能够提高超氧化物歧化酶(SOD)蛋白水平。对肺组织丙二醛(MDA)含量分析发现,与COPD组比较,PME治疗后明显抑制COPD小鼠肺组织中MDA的产生。Western blotting结果显示,PME治疗后能够阻止肺组织中的NF-κB抑制蛋白(IκBα)磷酸化以及NF-κB p65向细胞核转移,PME处理后的小鼠肺组织中Nrf2及其主要下游靶点血红素加氧酶1(HO-1)的表达明显升高。结论PME能够抑制COPD小鼠体内的炎症和氧化应激反应,改善脂多糖联合烟雾诱导肺组织损伤,其机制可能与激活Nrf2通路和抑制NF-κB通路相关。

关键词：贝母素甲慢性阻塞性肺疾病炎症氧化损伤免疫组织化学酶联免疫吸附剂测定免疫印迹法小鼠