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Effects of Barium Stress in Brassica juncea and Cakile maritima:The Indicator Role of Some Antioxidant Enzymes and Secondary Metabolites

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Phyton-International Journal of Experimental Botany 2021年第1期90卷 145-158页

作者： Houda Bouslimi Renata Ferreira Nesrine Dridi pedro brito Susete Martins-Dias Isabel Caçador Noomene Sleimi Laboratory of Resources Materials and EcosystemsFaculty of Sciences of BizerteUniversity of CarthageJarzounaBizerte7021Tunisia Centro de Recursos Naturais e Ambiente Instituto Superior TécnicoUniversidade de LisboaLisbon1049-001Portugal Instituto Português do Mare da Atmosfera Rua Dr.Alfredo Magalhães RamalhoLisbon1495-006Portugal Centro de Recursos Naturais e Ambiente DBEInstituto Superior TécnicoUniversidade de LisboaLisbon1049-001Portugal Marine and Environmental Sciences Centre Faculdade de CiênciasUniversidade de LisboaCampo GrandeLisbon1749-016Portugal

Soil contamination by toxic trace metal elements,like barium(Ba),may stimulate various undesirable changes in the metabolic activity of *** plant responses are fast and with,direct or indirect,generation of reactive oxygen species(ROS).To cope with the stress imposed by the ROS production,plants developed a dual cellular system composed of enzymatic and non-enzymatic players that convert ROS,and their by-products,into stable nontoxic *** assess the Ba stress response of two Brassicaceae species(Brassica juncea,a glycophyte,and Cakile maritime,a halophyte),plants were exposure to different Ba concentrations(0,100,200,300 and 500µM).The plants response was evaluated through their morphology and development,the determination of plant leaves antioxidant enzymatic activities and by the production of plants secondary *** indicated that the two Brassicaceae species have the ability to survive in an environment containing Ba(even at 500µM).The biomass production of *** was slightly affected whereas an increase in biomass *** was *** stress imposed by Ba activated the antioxidant defense system in the two species,noticed by the changes in the leaves activity of catalase(CAT),ascorbate peroxidase(APX)and guaicol peroxidase(GPX),and of the secondary metabolites,through the production of total phenols and *** enzymatic response was not similar within the two plant species:CAT and APX seem to have a more important role against the oxidative stress in *** while in *** is ***,total phenols and flavonoids production was more significant in the plants aerial part than in the roots,of the both *** the two Brassicaceae species response was different,in both plants catalytic and non-catalytic transformation of ROS occurs,and both were able to overcome the Ba toxicity and prevent the cell damage.

关键词： Brassica juncea Cakile maritima antioxidant enzymes barium stress oxidative stress secondary metabolites

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Functions of nuclear factor Y in nervous system development,function and health

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Neural Regeneration Research 2025年第10期20卷 2887-2894页

作者： pedro Moreira Roger Pocock Development and Stem Cells Program Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental BiologyMonash UniversityMelbourneVICAustralia

Nuclear factor Y is a ubiquitous heterotrimeric transcription factor complex conserved across eukaryotes that binds to CCAAT boxes,one of the most common motifs found in gene promoters and *** the last 30 years,research has revealed that the nuclear factor Y complex controls many aspects of brain development,including differentiation,axon guidance,homeostasis,disease,and most recently ***,a complete understanding of transcriptional regulatory networks,including how the nuclear factor Y complex binds to specific CCAAT boxes to perform its function remains *** this review,we explore the nuclear factor Y complex’s role and mode of action during brain development,as well as how genomic technologies may expand understanding of this key regulator of gene expression.

关键词： axon guidance CCAAT boxes neuronal degeneration neuronal differentiation neuronal regeneration nuclear factor Y complex transcription factor transcriptional regulation

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Brain scarring in infants:immunological insights from a neonatal hypoxic-ischemic encephalopathy model

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Neural Regeneration Research 2025年第10期20卷 2909-2910页

作者： pedro Moreno Pimentel-Coelho Carlos Chagas Filho Biophysics Institute Federal University of Rio de JaneiroRio de JaneiroBrazil

Neonatal hypoxic-ischemic encephalopathy(HIE)is a significant cause of disability in *** brain function and accelerating neurological recovery may require a combination of neuroprotective and pro-regenerative treatments at different stages of *** the first hours after the neonatal insult are the most critical period for neuroprotection,the existence of secondary and tertiary mechanisms of brain injury offers the possibility of preventing delayed neurodegeneration in the subsequent days,weeks,or months(Levison et al.,2022).

关键词： neonatal protective hypoxic

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Touristic urbanization and greening of coastal dune fields:A long-term assessment of a temperate sandy barrier of Argentina

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Journal of Geographical Sciences 2025年第1期 206-230页

作者： GARZO pedro Andrés DADON José Roberto ISLA Federico Ignacio Instituto de Investigaciones Marinas y Costeras (IIMyC-CONICET/UNMDP) Instituto de Geología de Costas y del Cuaternario Dr.Enrique J.Schnack (IGCC-CIC/UNMDP) Universidad de Buenos Aires Facultad de Arquitectura Dise?o y Urbanismo Centro de Investigaciones Gestión de Espacios Costeros (GEC-FADU/UBA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)

Dune barrier systems represent highly sought-after coastal landscapes for tourism and urban development around the world. However, a century ago, they were considered hazardous environments due to their great dynamic nature. As a result, stabilization practices were considered necessary. The systematic introduction of fast-growing exotic trees helped stabilize the sand, making it easier for tourism urbanization to take place, but also leading to erosion processes. This paper aims to assess long-term changes in vegetation cover over a large temperate barrier in Argentina. This complex region includes urban resorts, afforestation zones, and protected areas. A GIS-based geospatial analysis was conducted using a large satellite database(> 350 images), and the future evolution of the vegetation was modeled. The results revealed two primary spatiotemporal patterns associated with a gradual expansion of vegetation cover, accompanied by a concurrent reduction in sandy areas. In 1986, the dune area comprised 75% more surface than vegetation, whereas in 2021, it represented 60% less than vegetation. Furthermore, the 2050 scenario suggests a potential 40% reduction of dunes in certain areas. It is necessary to enhance management actions aimed at maintaining dune mobility and ensuring local and regional sediment balance. Long-term management strategies must focus on restoring native plant communities and controlling invasive species, and avoiding new dune fixation initiatives based on the introduction of exotic species.

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A multi-integrated approach on toxicity effects of engineered TiO2 nanoparticles

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Frontiers of Environmental Science & Engineering 2015年第5期9卷 793-803页

作者： Ana PICADO Susana M. PAIXAO Liliana MOITA Luis SILVA Mario S. DINIZ Joana LOURENCO Isabel PERES Luisa CASTRO Jose brito CORREIA Joana PEREIRA Isabel FERREIRA Antonio pedro Alves MATOS pedro BARQUINHA Elsa MENDONCA LNEG-National Laboratory of Energy and Geology I.P. Lisbon 1649-038 Portugal REQUIMTE Chemistry Department Fine Chemistry and Biotechnology Center Faculty of Sciences and Technology New University of Lisbon Caparica 2829-516 Portugal IMAR-Ocean Institute Department of Sciences and Environmental Engineering Faculty of Sciences and Technology New University of Lisbon Caparica 2829-516 Portugal CENIMAT/I3N and Department of Materials Science Faculty of Sciences and Technology New University of Lisbon Caparica 2829-516 Portugal Pathological Anatomy Curry Cabml Hospital Lisbon 1069-166 Portugal CESAM Faculty of Sciences Lisbon University Lisbon 1749-016 Portugal

The new properties of engineered nanoparti- cles drive the need for new knowledge on the safety, fate, behavior and biologic effects of these particles on organisms and ecosystems. Titanium dioxide nanoparticles have been used extensively for a wide range of applica- tions, e.g, self-cleaning surface coatings, solar cells, water treatment agents, topical sunscreens. Within this scenario increased environmental exposure can be expected but data on the ecotoxicological evaluation ofnanoparticles are still scarce. The main purpose of this work was the evaluation of effects of TiO2 nanoparticles in several organisms, covering different trophic levels, using a battery of aquatic assays. Using fish as a vertebrate model organism tissue histological and ultrastructural observations and the stress enzyme activity were also studied. TiO2 nanoparticles （Aeroxide~ P25）, two phase composition ofanatase （65%） and rutile （35%） with an average particle size value of 27.64-11 nm were used. Results on the ECs0 for the tested aquatic organisms showed toxicity for the bacteria, the algae and the crustacean, being the algae the most sensitive tested organism. The aquatic plant Lemna minor showed no effect on growth. The fish Carassius auratus showed no effect on a 21 day survival test, though at a biochemical level the cytosolic Glutathione-S-Transferase total activity, in intestines, showed a general significant decrease （p〈0.05） after 14 days of exposure for all tested concentrations. The presence of TiO2 nanoparticles aggregates were observed in the intestine lumen but their internalization by intestine cells could not be confirmed.

关键词： ecotoxicity enzymatic analysis histology,transmission electron microscopy (TEM) TiO2-nanoparti-cles

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Integrated risk management: a Petrobras application in offshore well construction safety to minimize critical emergency disconnections

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Petroleum Science 2018年第2期15卷 421-431页

作者： Claudio Benevenuto de Campos Lima pedro Esteves Aranha Jose Francisco Tebaldi de Castro Gilson brito Alves Lima Augusto Borella Hougaz Felipe de Souza Terra Petrobras Department of Production Engineering Federal Fluminense University

In order to avoid mistakes and to save a great deal of time in analysis, an innovative methodology was developed that can analyze the well operations and rig characteristics involved to define the best emergency disconnect sequence （EDS） available. A solution was developed based on the characteristics of the rigs and blowout preventers （BOPs）, and six variables were considered that directly affect the choice of EDS. All possible combinations of 64 scenarios were analyzed, and the priority of choice of the EDS was defined empirically. This paper presents an approach to EDS risk management and examples of exposure time （time without riser safety margin and shear capability） for the same well, which can be lowered from 13% to 0.1%. The impact of this reduction is related to the ability of the BOP to cut some of the heavy casings, in addition to improved availability of EDS modes. This implementation opened up many possibilities for the performance of risk exposure analysis, enabling comparison of several BOP configurations of contracted rigs and selection of the best options. This innovative approach allowed a better management of the rig schedules, prioritizing safety aspects and making it possible to allocate the fleet in a systematic way.

关键词： Well safety Safety barriers Emergency disconnect sequence (EDS) Well control Kick Well design

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QUANTUM FINANCIAL ECONOMICS——RISK AND RETURNS

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Journal of Systems Science & Complexity 2013年第2期26卷 187-200页

作者： GONCALVES Carlos pedro Instituto Superior de Ciencias Socials e Politicas-Technical University of Lisbon

Financial volatility risk and its relation to a business cycle-related intrinsic time is addressed through a multiple round evolutionary quantum game equilibrium leading to turbulence and multifractal signatures in the financial returns and in the risk *** model is simulated and the results are compared with actual financial volatility data.

关键词： Business cycle dynamics intrinsic time quantum chaotic volatility quantum financialeconomics.

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Distributed Energy Resource Scheduling with Focus on Demand Response Complex Contracts

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Journal of Modern Power Systems and Clean Energy 2021年第5期9卷 1172-1182页

作者： pedro Faria Zita Vale GECAD–Research Group on Intelligent Engineering and Computing for Advanced Innovation and Development R.Dr.António Bernardino de Almeida4314249-015PortoPortugal Polytechnic of Porto R.Dr.António Bernardino de Almeida4314249-015PortoPortugal

Demand response(DR)is a flexible way to improve distributed energy resource *** innovative contribution of this paper is to include complex contracts in the model,which can accommodate the constraints according to the special expectations of each *** contracts are included in the optimization of distributed energy resource scheduling to dispatch DR according to the expectations of ***-period DR events are *** this way,consumers can specify the limits on the time,power,and remuneration regarding participation in DR events,which has not been considered in the *** state of the art treats these aspects separately or uses a statistical approach,without providing consumers with options to combine their preferences regarding different aspects of their flexibility *** model has been validated for 218 consumers using several scenarios and different types of distributed generation,showing that it is possible to increase DR with respect to the preferences of consumers.

关键词： Complex contracts demand response distributed generation virtual power player

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P2X7 receptor blockade decreases inflammation,apoptosis,and enteric neuron loss during Clostridioides difficile toxin A-induced ileitis in mice

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World Journal of Gastroenterology 2022年第30期28卷 4075-4088页

作者： Ana A Q A Santos Deiziane V S Costa Danielle A Foschetti Antoniella S G Duarte Conceicao S Martins pedro M G Soares Patricia Castelucci Gerly A C brito Department of Morphology School of MedicineFederal University of CearaFortaleza 60430-170CearaBrazil Department of Physiology and Pharmacology School of MedicineFederal University of CearáFortaleza 60430-170CearaBrazil Department of Pathology and Legal Medicine School of MedicineFederal University of CearaFortaleza 60430-170CearaBrazil Department of Morphology(UFC) Federal University of CearaFortaleza 60430-170CearaBrazil Department of Anatomy Institute of Biomedical SciencesUniversity of São PauloSao Paulo 05508-270Brazil Department of Morphology Federal University of CearaFortaleza 60140-170CearaBrazil

Clostridioides difficile(***)is the most common pathogen causing health care-associated *** TcdA and TcdB have been shown to activate enteric neurons;however,what population of these cells is more profoundly influenced and the mechanism underlying these effects remain *** To characterize a specific population of TcdA-affected myenteric neurons and investigate the role of the P2X7 receptor in TcdA-induced ileal inflammation,cell death,and the changes in the enteric nervous system in *** Swiss mice were used to model TcdA-induced ileitis in ileal loops exposed to TcdA(50μg/Loop)for 4 *** investigate the role of the P2X7 receptor,Brilliant Blue G(50 mg/kg,i.p.),which is a nonspecific P2X7 receptor antagonist,or A438079(0.7μg/mouse,i.p.),which is a competitive P2X7 receptor antagonist,were injected one hour prior to TcdA *** samples were collected to analyze the expression of the P2X7 receptor(by quantitative real-time polymerase chain reaction and immunohistochemistry),the population of myenteric enteric neurons(immunofluorescence),histological damage,intestinal inflammation,cell death(terminal deoxynucleotidyltransferasemediated dUTP-biotin nick end labeling),neuronal loss,and S100B synthesis(immunohistochemistry).RESULTS TcdA upregulated(P<0.05)the expression of the P2X7 receptor gene in the ileal tissues,increasing the level of this receptor in myenteric neurons compared to that in control *** with the control mice indicated that TcdA promoted(P<0.05)the loss of myenteric calretinin+(Calr)and choline acetyltransferase+neurons and increased the number of nitrergic+and Calr+neurons expressing the P2X7 *** of the P2X7 receptor decreased TcdAinduced intestinal damage,cytokine release[interleukin(IL)-1β,IL-6,IL-8,and tumor necrosis factor-α],cell death,enteric neuron loss,and S100B synthesis in the mouse *** Our findings demonstrated that TcdA induced the upregulation of the P

关键词： Clostridioides difficile Clostridioides difficile toxin A P2X7 receptor Enteric nervous system Enteric neuron Enteric glia

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CAPTURING COMPLEX,NON-LINEAR TEAM BEHAVIOURS DURING COMPETITIVE FOOTBALL PERFORMANCE

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Journal of Systems Science & Complexity 2013年第1期26卷 62-72页

作者： DUARTE Ricardo ARAUJO Duarte FOLGADO Hugo ESTEVES pedro MARQUES pedro DAVIDS Keith Faculty of Human Kinetics Technical University of Lisbon School of Science and Technology University of Evora Department of Performance Analysis Manchester City FCUnited Kingdom School of Human Movement Studies Queensland University of Technologies

This study investigated changes in the complexity （magnitude and structure of variability） of the collective behaviours of association football teams during competitive performance. Raw positional data from an entire competitive match between two professional teams were obtained with the ProZone tracking system. Five compound positional variables were used to investigate the collective patterns of performance of each team including： surface and geometrical centre. Analyses involve the coefficient （ApEn）, as well as the linear association between both area, stretch index, team length, team width, of variation （%CV） and approximate entropy parameters. Collective measures successfully captured the idiosyncratic behaviours of each team and their variations across the six time periods of the match. Key events such as goals scored and game breaks （such as half time and full time） seemed to influence the collective patterns of performance. While ApEn values significantly decreased during each half, the %CV increased. Teams seem to become more regular and predictable, but with increased magnitudes of variation in their organisational shape over the natural course of a match.

关键词： Association football complexity social collective system variability.

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