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Earthquake Engineering and Engineering Vibration 2021年第1期20卷 245-256页

作者： Jesus Fernandez-Ruiz Luis E.Medina Rodriguez pedro alves costa Margarita Martinez-Diaz University of La Coruna Department of Civil EngineeringCampus de ElvinaLa Coruna 15071Spain University of Porto Faculty of EngineeringRua Dr.Roberto Friass/n Porto4200-465Portugal University of La Coruna Department of Naval and Industrial EngineeringCampus de ElvinaLa Coruna 15071Spain

In the last 30 years,the scientific community has developed and proposed different models and numerical approaches for the study of vibrations induced by railway *** of them are formulated in the frequency/wave number domain and with a 2.5D ***-dimensional numerical models formulated in the time/space domain are less frequently used,mainly due to their high computational ***,these models present very attractive characteristics,such as the possibility of considering nonlinear behaviors or the modelling of excess pore pressure and non-homogeneous and non-periodic geometries in the longitudinal direction of the *** this study,two 3D numerical approaches formulated in the time/space domain are compared and experimentally *** first one consists of a finite element approach and the second one of a finite difference *** experimental validation in an actual case situated in Carregado(Portugal)shows an acceptable fitting between the numerical results and the actual measurements for both ***,there are some differences among *** study therefore includes some recommendations for their use in practical soil dynamics and geotechnical engineering.

关键词： railway vibrations time/space domain 3D numerical model finite difference method implicit finite element method

Dose-response effect of pre-exercise carbohydrates under muscle glycogen unavailability:Insights from McArdle disease

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Journal of Sport and Health Science 2024年第3期13卷 398-408页

作者： pedro L.Valenzuela Alfredo Santalla Lidia B.Alejo Andrea Merlo Asuncion Bustos Laura Castellote-Belles Roser Ferrer-costa Nicola A.Maffiuletti David Barranco-Gil Tomas Pinos Alejandro Lucia Physical Activity and Health Research Group(“PaHerg”) Research Institute of Hospital“12 de Octubre”(“imas12”)Madrid 28041Spain Department of Systems Biology University of AlcalaMadrid 28871Spain Department of Sport and Computer Science Section of Physical Education and SportsFaculty of SportUniversidad Pablo de OlavideSevilla 41013Spain EVOPRED Research Group Universidad Europea de CanariasTenerife 38300Spain Faculty of Sport Sciences Universidad Europea de MadridMadrid 28670Spain Gait&Motion Analysis Laboratory Sol et Salus HospitalTorre Pedrera di Rimini(RN)47922Italy Department of Clinical Biochemistry Laboratoris ClınicsHospital Universitari Vall d’HebronBarcelona 08035Spain Human Performance Lab Schulthess ClinicZurich 8008Switzerland Biomedical Research Networking Center on Rare Disorders(CIBERER) Barcelona 08035Spain Mitochondrial and Neuromuscular Disorders Unit Vall d’Hebron Institut de RecercaUniversitat Autonoma de BarcelonaBarcelona 08035Spain

Background:This study aimed to determine the effect of different carbohydrate(CHO)doses on exercise capacity in patients with McArdle disease—the paradigm of“exercise intolerance”,characterized by complete muscle glycogen unavailability—and to determine whether higher exogenous glucose levels affect metabolic responses at the McArdle muscle cell(in vitro)***:Patients with McArdle disease(n=8)and healthy controls(n=9)underwent a 12-min submaximal cycling constant-load bout followed by a maximal ramp test 15 min after ingesting a non-caloric *** a randomized,double-blinded,cross-over design,patients repeated the tests after consuming either 75 g or 150 g of CHO(glucose:fructose=2:1).Cardiorespiratory,biochemical,perceptual,and electromyographic(EMG)variables were ***,glucose uptake and lactate appearance were studied in vitro in wild-type and McArdle mouse myotubes cultured with increasing glucose concentrations(0.35,1.00,4.50,and 10.00 g/L).Results:Compared with controls,patients showed the“classical”second-wind phenomenon(after prior disproportionate tachycardia,myalgia,and excess electromyographic activity during submaximal exercise,all p<0.05)and an impaired endurance exercise capacity(-51%ventilatory threshold and55%peak power output,both p<0.001).Regardless of the CHO dose(p<0.05 for both doses compared with the placebo),CHO intake increased blood glucose and lactate levels,decreased fat oxidation rates,and attenuated the second wind in the ***,only the higher dose increased ventilatory threshold(+27%,p=0.010)and peak power output(+18%,p=0.007).In vitro analyses revealed no differences in lactate levels across glucose concentrations in wild-type myotubes,whereas a doseresponse effect was observed in McArdle ***:CHO intake exerts beneficial effects on exercise capacity in McArdle disease,a condition associated with total muscle glycogen *** of these benefits are dose dependent.

关键词： Endurance Glycogen storage disease Glycogenosis Nutrition Supplement

Heme as an inducer of cerebral damage in hemorrhagic stroke:potential therapeutic implications

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Neural Regeneration Research 2022年第9期17卷 1961-1962页

作者： Luiz Ricardo da costa Vasconcellos pedro Moreno Pimentel-Coelho Cellular Signalling and Cytoskeletal Function Laboratory The Francis Crick InstituteLondonUK Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de JaneiroInstituto Nacional de Ciência e Tecnologia em Medicina RegenerativaRio de JaneiroRJBrazil

Intracerebral hemorrhage(ICH)consists of the rupture of a cerebral artery leading to bleeding into the surrounding *** event has a primary phase of brain injury consisting of mechanical tissue damage due to the mass effect,followed by a secondary phase of brain injury triggered by the presence of blood components released at the site of bleeding(Bulters et al.,2018).Despite the high rates of mortality and morbidity from ICH,no effective treatment is available so far.

关键词： cerebral damage rupture

Effect of increasing densities of Urochloa brizantha cv. Marandu on Eucalyptus urograndis initial development in silvopastoral system

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Journal of Forestry Research 2019年第2期30卷 537-543页

作者： Michel Anderson Almeida Colmanetti Allan Lopes Bacha pedro Luis da costa Aguiar alves Rinaldo Cesar de Paula University of Sao Paulo – USP/ESALQ Sao Paulo State University – UNESP/FCAV

In silvopasture system, the coexistence of eucalyptus seedlings with other species may result in growth reduction, especially during eucalyptus early ***, studies elucidating how forage species affect the eucalyptus growth can provide important information for their rational management aiming to obtain the maximum gain of the system. The aim of this work was to evaluate the effect of increasing densities of Urochloa brizantha *** in the early development of Eucalyptus urograndis. An experiment was conducted in 20 L pots, in an open and semi-controlled area, during 90 days after planting of eucalyptus. A completely randomized design with four replications was used, in a 6 9 7 factorial system, meaning six evaluation periods and seven densities of U. brizantha: 0(control), 22, 33, 44, 67, 89 and111 plants m^(-2). Fortnightly, eucalyptus height, stem diameter and chlorophyll fluorescence(Fv/Fm) were evaluated. At the end of experimental period, the net assimilation rate, stomatal conductance and transpiration rate of eucalyptus plants were determined, in addition to the dry matter of eucalyptus(leaves and stem) and U. brizantha (leaves). In coexistence with 111 plants m^(-2), eucalyptus had reduction of 63.9% on total dry matter and 72.7% on leaf area, compared to the control. From the density of22 plants m^(-2), U. brizantha negatively interfere significantly the growth of E. urograndis. Up to 8 plants m^(-2) there are no reductions greater than 5% in eucalyptus height and stem diameter.

关键词： Eucalyptus Palisade grass Competition Interference

P2X7 receptor blockade decreases inflammation,apoptosis,and enteric neuron loss during Clostridioides difficile toxin A-induced ileitis in mice

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World Journal of Gastroenterology 2022年第30期28卷 4075-4088页

作者： Ana A Q A Santos Deiziane V S costa Danielle A Foschetti Antoniella S G Duarte Conceicao S Martins pedro M G Soares Patricia Castelucci Gerly A C Brito Department of Morphology School of MedicineFederal University of CearaFortaleza 60430-170CearaBrazil Department of Physiology and Pharmacology School of MedicineFederal University of CearáFortaleza 60430-170CearaBrazil Department of Pathology and Legal Medicine School of MedicineFederal University of CearaFortaleza 60430-170CearaBrazil Department of Morphology(UFC) Federal University of CearaFortaleza 60430-170CearaBrazil Department of Anatomy Institute of Biomedical SciencesUniversity of São PauloSao Paulo 05508-270Brazil Department of Morphology Federal University of CearaFortaleza 60140-170CearaBrazil

Clostridioides difficile(***)is the most common pathogen causing health care-associated *** TcdA and TcdB have been shown to activate enteric neurons;however,what population of these cells is more profoundly influenced and the mechanism underlying these effects remain *** To characterize a specific population of TcdA-affected myenteric neurons and investigate the role of the P2X7 receptor in TcdA-induced ileal inflammation,cell death,and the changes in the enteric nervous system in *** Swiss mice were used to model TcdA-induced ileitis in ileal loops exposed to TcdA(50μg/Loop)for 4 *** investigate the role of the P2X7 receptor,Brilliant Blue G(50 mg/kg,i.p.),which is a nonspecific P2X7 receptor antagonist,or A438079(0.7μg/mouse,i.p.),which is a competitive P2X7 receptor antagonist,were injected one hour prior to TcdA *** samples were collected to analyze the expression of the P2X7 receptor(by quantitative real-time polymerase chain reaction and immunohistochemistry),the population of myenteric enteric neurons(immunofluorescence),histological damage,intestinal inflammation,cell death(terminal deoxynucleotidyltransferasemediated dUTP-biotin nick end labeling),neuronal loss,and S100B synthesis(immunohistochemistry).RESULTS TcdA upregulated(P<0.05)the expression of the P2X7 receptor gene in the ileal tissues,increasing the level of this receptor in myenteric neurons compared to that in control *** with the control mice indicated that TcdA promoted(P<0.05)the loss of myenteric calretinin+(Calr)and choline acetyltransferase+neurons and increased the number of nitrergic+and Calr+neurons expressing the P2X7 *** of the P2X7 receptor decreased TcdAinduced intestinal damage,cytokine release[interleukin(IL)-1β,IL-6,IL-8,and tumor necrosis factor-α],cell death,enteric neuron loss,and S100B synthesis in the mouse *** Our findings demonstrated that TcdA induced the upregulation of the P

关键词： Clostridioides difficile Clostridioides difficile toxin A P2X7 receptor Enteric nervous system Enteric neuron Enteric glia

Assessing potential protection effects on commercial fish species in a Cuban MPA

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Aquaculture and Fisheries 2020年第5期5卷 234-244页

作者： Barbara Horta e costa Jorge Angulo-Valdés Jorge M.S.Gonçalves pedro Barros Center of Marine Sciences CCMARUniversity of AlgarveCampus de GambelasFaro8005-139Portugal Centro de Investigaciones Marinas Universidad de La HabanaCalle 16 No.114PayaLa HabanaCP 11300Cuba School of Natural Resources and Environment University of FloridaUnited States of America103 Black HallGainesvilleFL32611USA Centre for Marine and Environmental Research(CIMA) University of AlgarveCampus de GambelasFaro8005-139Portugal Food and Agriculture Organization(FAO) Fisheries and Aquaculture Department Marine and Inland Fisheries Branch(FIRF)Rome00153Italy

Cuba has been leading marine protected area(MPA)designation in the Caribbean region to ensure conservation of its valuable marine ***,an efficient monitoring program for MPAs is still to be implemented and will benefit from pre-existing *** highly regulated MPA of Punta Francés National Park is one of the first Cuban MPAs and was established mainly to protect species and ecosystems for tourism *** of protection effects on commercial fish species was lacking in this *** study aimed at increasing local scientific knowledge by providing a baseline study about the most commercially fished families(Haemulidae,Lutjanidae and Serranidae)around Punta Francés *** collected represent only a limited period but can be used as a base point to support future *** abundance in number and biomass,and size were collected to test for differences between inside and adjacent areas outside the Punta Francés MPA,in different coral reef *** main result of this study was the significantly larger size and biomass of snappers and groupers outside the MPA where intense fisheries *** observation consistent with a large spawning aggregation was also recorded outside the *** with a limited set of data,these results suggest that,at least temporarily,the most targeted species[and sizes]are still highly vulnerable to *** habitats in the adjacent area,that are apparently missing within the MPA,may support some of the results *** the limits of the Punta Francés MPA to include those habitats outside may be critical to ensure the effectiveness of this MPA in contributing to protect the most commercial species of the *** should be done together with efficient fisheries management measures in the region,such as the significantly increase of minimum legal sizes and temporal closures during spawning migrations.

关键词： Cuba Marine protected areas Commercial fish species Spawning aggregations Artisanal fisheries Coral reefs

RNA Polymerase V Functions in Arabidopsis Interphase Heterochromatin Organization Independently of the 24-nt siRNA-Directed DNA Methylation Pathway

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Molecular Plant 2009年第4期2卷 700-710页

作者： Olga Pontes pedro costa-Nunes Paul Vithayathil Craig S. Pikaard Biology Department Washington University 1 Brookings Drive St Louis MO 63130 USA

In Arabidopsis, pericentromeric repeats, retroelements, and silenced rRNA genes are assembled into heterochromatin within nuclear structures known as chromocenters. The mechanisms governing higher-order heterochromatin organization are poorly understood but 24-nt small interfering RNAs （siRNAs） are known to play key roles in heterochromatin formation. Nuclear RNA polymerase IV （Pol IV）, RNA-DEPENDENT RNA POLYMERASE 2 （RDR2）, and DICER-LIKE 3 （DCL3） are required for biogenesis of 24-nt siRNAs that associate with ARGONAUTE 4 （AGO4）. Nuclear RNA polymerase V （Pol V） collaborates with DRD1 （DEFICIENT IN RNA-DEPENDENT DNA METHYLATION 1） to generate transcripts at heterochromatic loci that are hypothesized to bind to siRNA-AGO4 complexes and subsequently recruit the de-novo DNA methylation and/or histone modifying machinery. Here, we report that decondensation of the major pericentromeric repeats and depletion of the heterochromatic mark histone H3 lysine 9 dimethylation at chromocenters occurs specifically in pol V and drdl mutants. Disruption of pericentromeric repeats condensation is coincident with transcriptional reactivation of specific classes of pericentromeric 180-bp repeats. We further demonstrate that Pol V functions independently of Pol IV, RDR2, and DCL3-mediated siRNA production to affect interphase heterochromatin organization, possibly by involving RNAs that recruit structural or chromatin-modifying proteins.

关键词： RNA polymerase V RNA-directed DNA methylation heterochromatin centromere gene expression.

Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases

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Animal Nutrition 2022年第2期9卷 184-192页

作者： Monica M.costa Luís B.Pio pedro Bule Vania A.Cardoso Marlene Duarte Cristina M.Alfaia Diogo F.Coelho Joana A.Bras Carlos M.G.A.Fontes Jose A.M.Prates CIISA-Centro de Investigaçao Interdisciplinar em Sanidade Animal Faculdade de Medicina Veterinari1Universidade de Lisbo1Alto da Ajud11300-477 Lisbo1Portugal NZYTech-Genes and Enzymes Estrada do Paço do LumiarCampus do LumiarEdifício E1649-038 Lisbo1Portugal

Green macroalgae,e.g.,Ulva lactuca,are valuable bioactive sources of nutrients;but algae recalcitrant cell walls,composed of a complex cross-linked matrix of polysaccharides,can compromise their utilization as feedstuffs for monogastric *** study aimed to evaluate the ability of pre-selected Carbohydrate-Active enZymes(CAZymes)and sulfatases to degrade *** cell walls and release nutritive compounds.A databank of 199 recombinant CAZymes and sulfatases was tested in vitro for their action towards *** cell wall *** enzymes were incubated with the macroalga,either alone or in combination,to release reducing sugars and decrease fluorescence intensity of Calcofluor White stained cell *** individual action of a polysaccharide lyase family 25(PL25),an ulvan lyase,was shown to be the most efficient in cell wall *** ulvan lyase treatment,in triplicate measures,promoted the release of 4.54 g/L(P0.05),proteins(P?0.861)or pigments(P>0.05)was *** results highlight the capacity of a single recombinant ulvan lyase(PL25 family)to incompletely disrupt *** cell *** enzyme could enhance the bioaccessibility of *** bioactive products with promising utilization in the feed industry.

关键词： Macroalga Ulva lactuca Carbohydrate-active enzyme Ulvan lyase Cell wall degradation

Exosomal glypican-1 is elevated in pancreatic cancer precursors and can signal genetic predisposition in the absence of endoscopic ultrasound abnormalities

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World Journal of Gastroenterology 2022年第31期28卷 4310-4327页

作者： pedro Moutinho-Ribeiro Ines A Batista Sofia T Quintas Bárbara Adem Marco Silva Rui Morais Armando Peixoto Rosa Coelho pedro costa-Moreira Renato Medas Susana Lopes Filipe Vilas-Boas Manuela Baptista Diogo Dias-Silva Ana L Esteves Filipa Martins Joanne Lopes Helena Barroca Fátima Carneiro Guilherme Macedo Sonia A Melo Serviço de Gastrenterologia Centro Hospitalar Universitário de Sao JoaoPorto 4200Portugal Faculty of Medicine University of PortoPorto 4200Portugal Instituto de Investigacao e Inovacao em Saúde(i3S) Universidade do PortoPorto 4200Portugal IPATIMUP-Institute of Molecular Pathology and Immunology University of PortoPorto 4200Portugal Instituto de Ciencias Biomédicas Abel Salazar(ICBAS) University of PortoPorto 4050Portugal Servico de Cirurgia Geral Centro Hospitalar Universitário de Sao JoaoPorto 4200Portugal Unidade de Saúde Familiar Serpa Pinto ACeS Porto OcidentalPorto 4250Portugal Serviço de Anatomia Patológica Centro Hospitalar Universitário de Sao JoaoPorto 4200Portugal

BACKGROUND Individuals within specific risk groups for pancreatic ductal adenocarcinoma(PDAC)[mucinous cystic lesions(MCLs),hereditary risk(HR),and new-late onset diabetes mellitus(NLOD)]represent an opportunity for early cancer *** ultrasound(EUS)is a premium image modality for PDAC screening and precursor lesion *** no specific biomarker is currently clinically available for this purpose,glypican-1(GPC1)is overexpressed in the circulating exosomes(crExos)of patients with PDAC compared with healthy subjects or those harboring benign pancreatic *** To evaluate the capacity of GPC1+crExos to identify individuals at higher risk within these specific groups,all characterized by *** This cross-sectional study with a prospective unicentric cohort included 88 subjects:40 patients with MCL,20 individuals with HR,and 20 patients with NLOD.A control group(CG)was submitted to EUS for other reasons than pancreatic pathology,with normal pancreas and absence of hereditary risk factors(n=8).The inclusion period was between October 2016 and January 2019,and the study was approved by the Ethics Committee of Centro Hospitalar Universitário de São João,Porto,*** patients provided written informed *** and blood tests for quantification of GPC1+crExos by flow cytometry and carbohydrate antigen 19-9(CA 19-9)levels by ELISA were performed in all ***-guided tissue acquisition was done whenever *** statistical analysis,SPSS®27.0(IBM Corp.,Armonk,NY,United States)version was *** graphs were created using GraphPad Prism 7.00(GraphPad Software,San Diego,CA,United States).RESULTS Half of MCLs harbored worrisome features(WF)or high-risk stigmata(HRS).Pancreatic abnormalities were detected by EUS in 10.0%and 35.0%in HR and NLOD individuals,respectively,all considered non-malignant and“harmless.”Median levels of GPC1+crExos were statistically different:MCL[99.4%,interquartile range(IQR):94.9%-99.8%],HR(8

关键词： Glypican-1 Circulating exosomes Endoscopic ultrasound Pancreatic cancer risk groups Pancreatic cancer precursor lesions Genetic predisposition

Nanotubes in a Gradient Electric Field as Revealed by STM TEM Technique

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Nano Research 2008年第2期1卷 166-175页

作者： Dmitri Golberg pedro M.F.J.costa Masanori Mitome Yoshio Bando Nanoscale Materials Center Namiki 1-1TsukubaIbaraki 305-0044Japan International Center for Materials Nanoarchitectonics(MANA) National Institute for Materials ScienceNamiki 1-1TsukubaIbaraki 305-0044Japan Center for Research in Ceramics and Composite Materials University of Aveiro3810-193 AveiroPortugal

We have investigated the behavior of two nanotube systems,carbon and boron nitride,under controlled applied voltages in a high-resolution transmission electron microscope(TEM)equipped with a scanning tunneling microscope(STM)*** nanotubes(or thin bundles)were positioned between a piezo-movable gold electrode and a biased(up to±140 V)STM tip inside the pole-piece of the *** structures studied include double-and multi-walled carbon nanotubes(the latter having diverse morphologies due to the various synthetic procedures utilized),few-layered boron nitride nanotube bundles and multi-walled boron nitride nanotubes(with or without functionalized surfaces).The electrical breakdown,physical failure,and electrostatic interactions are documented for each system.

关键词： Carbon nanotubes boron nitride nanotubes transmission electron microscope scanning tunneling microscope