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Liquid crystal immunosensors for the selective detection of Escherichia coli with a fast analysis tool

Photonics Research 2024年第7期12卷 1564-1573页

作者： SANDRO C.OLIvEIRA mARIA S.SOARES BÁRBARA v.GONÇALvES ANDREIA C.m.RODRIGUES AmADEU m.v.m.SOARES RITA G.SOBRAL NUNO F.SANTOS JAN NEDOmA pedro L.ALmEIDA CARLOS mARQUES CICECO-Aveiro Institute of materials Physics DepartmentUniversity of Aveiro3810-193 AveiroPortugal i3N Physics DepartmentUniversity of Aveiro3810-193 AveiroPortugal Associate Laboratory i4HB-Institute for Health and Bioeconomy School of Sciences and TechnologyNOVA University of Lisbon1099-085 LisbonPortugal CESAm-Centre for Environmental and marine Studies and Department of Biology University of Aveiro3810-193 AveiroPortugal Department of Telecommunications VSB-Technical University of OstravaOstrava 70800Czech Republic i3N-CENImAT School of Sciences and TechnologyNOVA University of Lisbon1099-085 LisbonPortugal UnIRE ISELPolytechnic Institute of Lisbon1549-020 LisbonPortugal Department of Physics VSB-Technical University of OstravaOstrava 70800Czech Republic

The consumption of contaminated food may cause serious illnesses,and traditional methods to detect Escherichia coli are still associated with long waiting times and high costs given the necessity to transport samples to specialized *** is a need to develop new technologies that allow cheap,fast,and direct monitoring at the site of ***,in this work,we developed optical immunosensors for the selective detection of ***,based on liquid crystal technology,whose molecules can align in different manners depending on the boundary conditions (such as substrates) as well as the environment that they *** glass substrate was functionalized with *** antibody using cysteamine as an intermediate,and a vertical alignment was imposed on the liquid crystal molecules by using DmOAP during *** presence of bacteria disrupts the alignment of the liquid crystal molecules,changing the intensity of light emerging between cross polarizers,measured using a polarized optical microscope and a *** was possible to detect *** in suspensions in the concentration range from 2.8 cells/mL to 2.8×10^(9) cells∕*** was also evaluated,and the sensors were used to analyze contaminated water samples.A prototype was developed to allow faster,in-situ,and easier analysis avoiding bulky instruments.

关键词： crystal alignment analysis

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CONSTANS alters the circadian clock in Arabidopsis thaliana

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molecular Plant 2024年第8期17卷 1204-1220页

作者： pedro de los Reyes Gloria Serrano-Bueno Francisco J.Romero-Campero He Gao Jose m.Romero Federico valverde Plant Development Group-Institute for Plant Biochemistry and Photosynthesis Consejo Superior de Investigaciones CientificasUniversidad de SevillaSevilleSpain Department of Computer Science and Artificial Intelligence Universidad de SevillaSevilleSpain Department of Plant Developmental Biology Max Planck Institute for Plant Breeding ResearchCologneGermany Department of Plant Biochemistry and molecular Biology Universidad de SevillaSevilleSpain

Plants are sessile organisms that have acquired highly plastic developmental strategies to adapt to the *** these processes,the floral transition is essential to ensure reproductive success and is finelyregulated by several internal andexternal genetic *** photoperiodic pathway,which controls plant response to day length,is one of the most important pathways controlling *** Arabidopsis photoperiodic flowering,CONSTANS(CO)is the central gene activating the expression of the florigen FLOWERING LOCUS T(FT)in the leaves at the end of a long *** circadian clock strongly regulates Co ***,to date,no evidence has been reported regarding a feedbackloop from the photoperiod pathway back to the circadian *** transcriptional networks,we have identified relevant network motifs regulating the interplay between the circadian clock and the photoperiod *** expression,chromatin immunoprecipitation experiments,and phenotypic analysis allowed us to elucidate the role of Co over the circadian *** with altered cO expression showed a different internal clock period,measured by daily leaf rhythmic *** showed that co upregulates the expression of key genes related to the circadian clock,such as CCA1,LHY,PRR5,and Gl,at the end of a long day by bindingto specific sites on their ***,a high numberof PRR5-repressed target genes are upregulated by CO,and this could explain the phase transition promoted by *** CO-PRR5 complex interacts with the bZiP transcriptionfactor HY5andhelps to localize the complex in the promoters of clock *** together,our results indicate that there may be a feedback loop in which co communicates back to the circadian clock,providing seasonal information to the circadian system.

关键词： CONSTANS circadian clock photoperiod

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Solar-assisted photocatalytic water splitting using defective UiO-66 solids from modulated synthesis

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Nano Research 2024年第5期17卷 4134-4150页

作者： Celia m.Rueda-Navarro maría Cabrero-Antonino Paula Escamilla valentín Díez-Cabanes Dong Fan pedro Atienzar Belén Ferrer Ignacio vayá Guillaume maurin Herme G.Baldoví Sergio Navalón Departamento de Química Universitat Politècnica de ValènciaCamino de Vera s/nValencia 46022Spain Instituto de Ciencia molecular(ICmOL) Universitat de ValènciaPaterna 46980Spain ICGm Univ.MontpellierCNRSENSCMMontpellier34095France Instituto de Tecnología Química(UPv-CSIC) Universitat Politècnica de ValènciaAvenida de los Naranjos s/nValencia 46022Spain

metal-organic frameworks(mOFs)are attracting increasing interests as photocatalysts for solar-driven hydrogen production from *** paper reports on a comparative study of using either acetic acid(AA)or trifluoroacetic acid(TFA)as the representative UiO-66 organic modulators for synthesizing visible light responsive UiO-66(Zr)-X(X:NH_(2) or NO_(2))photocatalysts for water *** results show that photocatalytic hydrogen generation from a water/methanol mixture can be improved by varying the nature and amount of the modulator employed to prepare the different UiO-66(Zr)-X(X:NH_(2) or NO_(2))solid ***-66(Zr)-NH_(2) was the most active photocatalyst,followed by UiO-66(Zr)-NO_(2),both prepared with 12 equivalents of AA with respect to the organic *** UiO-66(Zr)-NH_(2) solid was more active than the parent mOF in photocatalytic overall water splitting(OWS)(H_(2) and O_(2) production of 450 and 160μmol·g^(-1),respectively,in 5 h;apparent quantum yield(AQY)at 400 nm of 0.06%)in the absence of methanol and compares favourably with analogous *** on the photocatalytic activity of the most active solids of both series was obtained by means of a series of techniques,including ultraviolet-visible(Uv-vis)diffuse reflectance,X-ray photoelectron spectroscopy(XPS),laser flash photolysis(LFP),electron spin resonance(ESR),photoluminescence and photoelectrochemical measurements together with density functional theory(DFT)*** results showed that organic acid modulators can be used to enhance the photocatalytic activity of missing linker UiO-66 defective materials in solar-powered water splitting.

关键词： heterogeneous photocatalysis metal-organic frameworks(mOFs) UiO-66 materials carboxylic acid modulators water splitting solar light

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How does high-intensity intermittent training affect recreational endurance runners? Acute and chronic adaptations: A systematic review

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Journal of Sport and Health Science 2017年第1期6卷 54-67页

作者： Felipe García-Pinillos víctor m.Soto-Hermoso pedro A.Latorre-Román Department of Corporal Expression University of Jaen Department of Sports Sciences University of Granada

Objective: This systematic review aimed to critically analyze the literature to determine how high-intensity intermittent training(HIIT) affects recreational endurance runners in the short-and ***: Electronic databases were searched for literature dating from January 2000 to October 2015. The search was conducted using the key words "high-intensity intermittent training" or "high-intensity interval exercise" or "interval running" or "sprint interval training" and "endurance runners" or "long distance runners". A systematic approach was used to evaluate the 783 articles identified for initial review. Studies were included if they investigated HIIT in recreational endurance runners. The methodological quality of the studies was evaluated using the Physiotherapy Evidence Database(pedro) scale(for intervention studies) and the modified Downs and Black Quality Index(for cross-sectional studies).Results: Twenty-three studies met the inclusionary criteria for review. The results are presented in 2 parts: cross-sectional(n = 15) and intervention studies(n = 8). In the 15 cross-sectional studies selected, endurance runners performed at least 1 HIIT protocol, and the acute impact on physiological, neuromuscular, metabolic and/or biomechanical variables was assessed. Intervention studies lasted a minimum of 4 weeks, with 10 weeks being the longest intervention period, and included 2 to 4 HIIT sessions per week. most of these studies combined HIIT sessions with continuous run(CR) sessions; 2 studies' subjects performed HIIT ***: HIIT-based running plans(2 to 3 HIIT sessions per week, combining HIIT and CR runs) show athletic performance improvements in endurance runners by improving maximal oxygen uptake and running economy along with muscular and metabolic adaptations. To maximize the adaptations to training, both HIIT and CR must be part of training programs for endurance runners.

关键词： Endurance High-intensity training Intermittent exercises Interval running Long-distance runners Running

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Effects of 12 weeks of barefoot running on foot strike patterns,inversion-eversion and foot rotation in long-distance runners

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Journal of Sport and Health Science 2019年第6期8卷 579-584页

作者： pedro ALatorre-Román Felipe García-Pinillos víctor m.Soto-Hermoso marcos munoz-Jiménez Department of Didactics of Corporal Expression University of JaénJaen 23071Spain Department of Sports Sciences University of GranadaGranada 18071Spain

Purpose:The purpose of this study was to determine the effects of 12 weeks of barefoot running on foot strike patterns,inversion-eversion and foot rotation in long-distance ***:Thirty-one endurance runners with no experience in barefoot running were randomized into a control group and an experimental group who received barefoot *** pre-test and post-test,all subjects ran at low and high self-selected speeds on a *** were collected by systematic observation of lateral and back recordings at 240 ***:mcNemar’s test indicated significant changes(p mental group at both high and low speed running in foot strike patterns,reducing the percentage of high rearfoot strikers and increasing the number of midfoot strikers.A significant increase(p version occurred at comfortable speed in the experimental ***:Twelve weeks of barefoot running,applied progressively,causes significant changes in foot strike pattern with a tendency toward midfoot or forefoot strikes,regardless of running speed and significant changes in foot rotation at low speed,while the inversion was reduced in left foot at low speed with a tendency toward centered strike.

关键词： Endurance Footwear Kinematics Training Unshod

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Altitude as environmental filtering influencing phylogenetic diversity and species richness of plants in tropical mountains

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Journal of mountain Science 2023年第2期20卷 285-298页

作者： GALvÁN-CISNEROS Carlos m. vILLA pedro m. COELHO Alex J.P. CAmPOS Prímula v. mEIRA-NETO João A.A. Laboratory of Ecology and Evolution of Plants Departamento de Biologia VegetalUniversidade Federal de ViçosaViçosaMG36570-900Brazil Botany Gradute Program Universidade Federal de ViçosaViçosaMG36570-900Brazil

Elucidating how multiple factors affect biodiversity and plant community assembly is a central issue in ecology,especially in vulnerable ecosystems such as tropical *** studies are more relevant in global warming scenarios that induce the upward displacement of plant species towards reduced habitats and hostile environments in tropical *** study aimed to analyze how altitude affects taxonomic and phylogenetic diversity in plant communities of tropical ***,we tested if(i)increased altitude works as an environmental filtering promoting decreased species richness,decreased phylogenetic diversity,and increased phylogenetic clustering in these tropical mountains;and if(ii)plant communities of high altitude in tropical mountains are also result of recent diversification with plant species recently split shortening phylogenetic distances between closest related *** tested effects of altitude on species richness and phylogenetic metrics using linear mixed-effects *** Haleakala presented 114 species,mount Kilimanjaro presented 231 species and mount Purace presented 280 *** found an environmental filtering effect with increasing altitude causing phylogenetic clustering,decreased phylogenetic diversity and decreased species *** decreasing phylogenetic distances between closest relatives are congruent with neo-endemics,suggesting recent plant diversification in high altitudes of tropical mountains,possibly driven by geographic isolation and environmental *** of global warming should be monitored in tropical mountains focusing on distribution shifts.

关键词： Tropical mountains Global warming Environmental filtering Phylogenetic ecology Assembly rules Conservation mountaintop vegetation

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Significance of the balance between intracellular glutathione and polyethylene glycol for successful release of small interfering RNA from gold nanoparticles

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Nano Research 2015年第10期8卷 3281-3292页

作者： mark mcCully Yulan Hernandez Joao Conde pedro v. Baptista Jesus m. de la Fuente Andrew Hursthouse David Stirling Catherine C. Berry CentreforCellEngineering UniversityofGlasgowGlasgowG128QQScotlandUK InstituteofNanoscienceofAragon UniversityofZaragozaZaragoza50018Spain massachusettsInstituteofTechnology InstituteforMedicalEngineeringandScienceHarvard-MITDivisionforHealthSciencesandTechnologyCambridgeMassachusetts02139USA UCIBIO DepartamentodeCienciasdaVidaFaculdadedeCienciaseTecnologiaUniversidadeNovadeLisboaCampusdeCaparicaCaparica2829-516Portugal InstitutodeCienciadematerialesdeAragon(ICmA) CSIC-UniversidaddeZaragozac/PedroCerbuna12Zaragoza50009Spain InstituteofNanoBiomedicineandEngineering KeyLaboratoryforThinFilmandMicrofabricationTechnologyoftheMinistryofEducationResearchInstituteofTranslationMedicineShanghaiJiaoTongUniversityDongchuanRoad800Shanghai200240China SchoolofScience UniversityoftheWestofScotlandPaisleyPA12BEScotlandUK

The therapeutic promise of small interfering RNAs （siRNAs） for specific gene silencing is dependent on the successful delivery of functional siRNAs to the cytoplasm. Their conjugation to an established delivery platform, such as gold nanoparticles, offers tremendous potential for treating diseases and advancing our understanding of cellular processes. Their success or failure is dependent on both the uptake of the nanoparticles into the cells and subsequent intracellular release of the functional siRNA. In this study, utilizing gold nanoparticle siRNA-mediated delivery against C-mYC, we aimed to determine if we could achieve knockdown in a cancer cell line with low levels of intracellular glutathione, and determine the influence, if any, of polyethylene glycol （PEG） ligand density on knockdown, with a view to determining the optimal nanoparticle design to achieve C-mYC knockdown. We demonstrate that, regardless of the PEG density, knockdown in cells with relatively low glutathione levels can be achieved, as well as the possible effect of steric hindrance of PEG on the availability of the siRNA for cleavage in the intracellular environment. Gold nanoparticle uptake was demonstrated via transmission electron microscopy and mass spectroscopy, while knockdown was determined at the protein and physiological levels （cells in S-phase） by in-cell westerns and BrdU incorporation, respectively.

关键词： gold nanoparticles PEG glutathione siRNA drug delivery

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Delving into the variations in tree species composition and richness across south american subtropical atlantic and Pampean forests

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Journal of Plant Ecology 2015年第3期8卷 242-260页

作者： Ary T.Oliveira-Filho Jean C.Budke João A.Jarenkow pedro v.Eisenlohr Danilo R.m.Neves Departamento de Botânica Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisAv.Antônio Carlos6627Belo HorizonteMinas Gerais31270-901Brazil Departamento de Ciências Biológicas Universidade Regional Integrada do Alto Uruguai e das MissõesAv.Sete de Setembro1621ErechimRio Grande do Sul99700-000Brazil Departamento de Botânica Instituto de BiociênciasUniversidade Federal do Rio Grande do SulAv.Bento Gonçalves9500Porto AlegreRio Grande do Sul91501-970Brazil Royal Botanic Garden Edinburgh Inverleith RowEdinburghEH35LRUK

Aims We analyse here the variations in species composition and richness and the geographic ranges of the tree species occurring in south american subtropical atlantic and Pampean *** goals were to assess(i)the floristic consistency of usual classifications based on vegetation physiognomy,climate and elevation;(ii)the leading role of temperature-related variables on the variations in species composition and richness;(iii)the predominance of species with tropical-subtropical ranges,possibly as a result of forest expansion over grasslands after the last glacial maximum(LGm);(iv)the restriction of most subtropical endemics to stressful habitats as a possible result of past forest refuges during the *** The region was defined by the Tropic of Capricorn to the north,the rio de la Plata to the south,the atlantic shoreline to the east and the catchment areas of the upper Paranáand uruguay rivers to the *** analyses,multiple regression modelling and variance partition analyses were performed on a database containing 63994 occurrence records of 1555 tree species in 491 forest sites and 48 environmental *** species were also classified according to their known geographic *** Findingsa main differentiation in species composition and richness was observed between the eastern windward coastlands(rain and cloud forests)and western leeward hinterlands(Araucaria and semi-decid-uous forests).Pre-defined forest types on both sides were consistent with variations in tree species composition,which were significantly related to both environmental variables and spatial proximity,with extremes of low temperature playing a chief *** species rich-ness declined substantially towards the south and also from rain to seasonal forests and towards the highland summits and sandy *** richness was significantly correlated with both minimum temperature and actual *** 91%of the subtropical flora is shared with the

关键词： distribution patterns endemic species floristic connections macro-scale species richness spatial and climatic predictors subtropical forests

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Nanotubes in a Gradient Electric Field as Revealed by STm TEm Technique

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Nano Research 2008年第2期1卷 166-175页

作者： Dmitri Golberg pedro m.F.J.costa masanori mitome Yoshio Bando Nanoscale materials Center Namiki 1-1TsukubaIbaraki 305-0044Japan International Center for materials Nanoarchitectonics(mANA) National Institute for Materials ScienceNamiki 1-1TsukubaIbaraki 305-0044Japan Center for Research in Ceramics and Composite materials University of Aveiro3810-193 AveiroPortugal

We have investigated the behavior of two nanotube systems,carbon and boron nitride,under controlled applied voltages in a high-resolution transmission electron microscope(TEm)equipped with a scanning tunneling microscope(STm)*** nanotubes(or thin bundles)were positioned between a piezo-movable gold electrode and a biased(up to±140 v)STm tip inside the pole-piece of the *** structures studied include double-and multi-walled carbon nanotubes(the latter having diverse morphologies due to the various synthetic procedures utilized),few-layered boron nitride nanotube bundles and multi-walled boron nitride nanotubes(with or without functionalized surfaces).The electrical breakdown,physical failure,and electrostatic interactions are documented for each system.

关键词： Carbon nanotubes boron nitride nanotubes transmission electron microscope scanning tunneling microscope

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P2X7 receptor blockade decreases inflammation,apoptosis,and enteric neuron loss during Clostridioides difficile toxin A-induced ileitis in mice

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World Journal of Gastroenterology 2022年第30期28卷 4075-4088页

作者： Ana A Q A Santos Deiziane v S costa Danielle A Foschetti Antoniella S G Duarte Conceicao S martins pedro m G Soares Patricia Castelucci Gerly A C Brito Department of morphology School of MedicineFederal University of CearaFortaleza 60430-170CearaBrazil Department of Physiology and Pharmacology School of MedicineFederal University of CearáFortaleza 60430-170CearaBrazil Department of Pathology and Legal medicine School of MedicineFederal University of CearaFortaleza 60430-170CearaBrazil Department of morphology(UFC) Federal University of CearaFortaleza 60430-170CearaBrazil Department of Anatomy Institute of Biomedical SciencesUniversity of São PauloSao Paulo 05508-270Brazil Department of morphology Federal University of CearaFortaleza 60140-170CearaBrazil

Clostridioides difficile(***)is the most common pathogen causing health care-associated *** TcdA and TcdB have been shown to activate enteric neurons;however,what population of these cells is more profoundly influenced and the mechanism underlying these effects remain *** To characterize a specific population of TcdA-affected myenteric neurons and investigate the role of the P2X7 receptor in TcdA-induced ileal inflammation,cell death,and the changes in the enteric nervous system in *** Swiss mice were used to model TcdA-induced ileitis in ileal loops exposed to TcdA(50μg/Loop)for 4 *** investigate the role of the P2X7 receptor,Brilliant Blue G(50 mg/kg,i.p.),which is a nonspecific P2X7 receptor antagonist,or A438079(0.7μg/mouse,i.p.),which is a competitive P2X7 receptor antagonist,were injected one hour prior to TcdA *** samples were collected to analyze the expression of the P2X7 receptor(by quantitative real-time polymerase chain reaction and immunohistochemistry),the population of myenteric enteric neurons(immunofluorescence),histological damage,intestinal inflammation,cell death(terminal deoxynucleotidyltransferasemediated dUTP-biotin nick end labeling),neuronal loss,and S100B synthesis(immunohistochemistry).RESULTS TcdA upregulated(Pvel of this receptor in myenteric neurons compared to that in control *** with the control mice indicated that TcdA promoted(Pmyenteric calretinin+(Calr)and choline acetyltransferase+neurons and increased the number of nitrergic+and Calr+neurons expressing the P2X7 *** of the P2X7 receptor decreased TcdAinduced intestinal damage,cytokine release[interleukin(IL)-1β,IL-6,IL-8,and tumor necrosis factor-α],cell death,enteric neuron loss,and S100B synthesis in the mouse *** Our findings demonstrated that TcdA induced the upregulation of the P

关键词： Clostridioides difficile Clostridioides difficile toxin A P2X7 receptor Enteric nervous system Enteric neuron Enteric glia

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