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Characterization and Comparison of Microbial Soil Diversity in Two Andean Peatlands in Different States of Conservation-Vega Tocorpuri

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Journal of Geoscience and Environment Protection 2018年第4期6卷 194-210页

作者： Carolina Belfiore Ana Fernandez Ana Paula santos Manuel Contreras María Eugenia Farías Laboratorio de Investigaciones Microbiològicas de Lagunas Andinas (LIMLA) Planta Piloto de Procesos Industriales Microbiol&#242gicos (PROIMI-CONICET) Tucuma&#225n Argentina Centro de Ecologiía Aplicada (CEA) Suecia Chile

Cerro Tocorpuri, belongs to the II region of Chile, in San pedro de Atacama, on the border of Chile-Bolivia. The presence of a more or less constant supply of water conditions the existence of characteristic vegetation systems known as bogs (bofedales, vegas and marshes). These wetlands have a cultural, environmental and economic social importance. As a result of the exploitation of aquatic rights, peatlands began to dry up with the consequent loss of natural resources and damage to ancestral rights, and natural resources. The activities of microorganisms in wetlands play an important role in biogeochemical processes. The interaction between microbial diversity and soil, influences to the ability of the ecosystem to recover from stress (resilience). In the present work, the soil characteristics and the associated microbial biodiversity were studied, comparing samples of active and deteriorated peatland. It was seen that the loss of water causes great changes in the physical-chemical characteristics of the soil, which leads to a modification of the microbiota Proteobacteria decreased by 18% in deteriorated peatlands, which are evident more sensible to extreme conditions while Acidobacteria, Actinobacteria increased in these sample showing a better adaptation to the change of conditions. In view of the fact that high Andean Peatlands are exposed to increasing environmental impact, this preliminary comparative study of pristine and altered soil could guide the research directed to recovery of dead peatlands strategies.

关键词： Peatland Microbial Diversity Altered Soil

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Nodular Amyloidosis Overlapping and Polymyositis in a Patient with Skin Manifestations Lupus Fax: Case Report

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Journal of Biosciences and Medicines 2017年第11期5卷 33-40页

作者： Eneas Van Der Maas do Bem Filho Laiza Maria dos santos Couto Antonio Luiz da Costa Soares Jr pedro Paulo Andrade Thiago Sande Miguel Sebastiao Celio Horta Coelho Filho Daniel Almeida da Costa Livia Cristina de Melo Pino Thayro Van Der Maas do Bem The Medical School at the Center of Higher Education of Valenca ValencaBrazil

Amyloidosis is the result of fibrous and insoluble amyloid protein deposition in extracellular spaces of tissues and organs, including the skin. Through the analysis of medical records, clinical condition and laboratory tests, this article proposes a rare case report of an adult patient with symptoms and diagnosis of polymyositis, histopathology of nodular amyloidosis with skin lesions suggestive of lupus. The patient is still being followed at the dermatology clinic of Valença Medical School, in order to improve the prognosis and prevent the progression of existing symptoms.

关键词： Nodular Amyloidosis Polymyositis

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Subcutaneous Model for the Study of Dengue Virus Infection in Immune Competent Mice

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Journal of Biosciences and Medicines 2018年第5期6卷 97-110页

作者： Beatriz Senra santos Natalia Lima Pessoa Natalia Lucinda Gustavo Cardoso de Oliveira Thais Souza Silva Ketyllen Reis Andrade Ketyllen Reis Andrade Bruno Galvã o Filho Marcele Neves Rocha Alexandre Vieira Machado pedro Augusto Alves É rica Alessandra Rocha Alves Erna Geessien Kroon Marco Antô nio Campos Instituto René Rachou Funda&amp amp ccedil & amp atilde o Oswaldo Cruz Fiocruz Belo Horizonte Brazil Departamento de Microbiologia Universidade Federal de Minas Gerais Belo Horizonte Brazil Empresa Brasileira de Pesquisa Agropecuá ria Laborat&amp amp oacute rio de Virologia Brasilia Brazil

Various mouse models to study dengue have been described by different authors, some of them using immunodeficient or some using humanized mice. Our group reported previously a deadly murine model, which used the intracranial inoculum of highly virulent Dengue virus (DENV) in immune competent mouse. Here we present a model of immune competent mouse (C57BL/6), infected subcutaneously by the same highly virulent DENV (DENV3 genotype I). In this immunocompetent systemic mice model, the cytokine levels and hematological parameters such as total and differential leukocyte and platelets counts, together with weight loss, were considered important monitoring parameters, allowing a better understanding of the systemic human disease. Mice were inoculated subcutaneously and evaluated by the percentage weight variation as well as the clinical signs. Hematological parameters and cytokines levels were measured and viral titration in brain tissue or serum neutralization was performed to confirm mice infection. The subcutaneously DENV inoculated mice showed weight loss after infection, but they did not show any other clinical signs. The leukocytes and platelets decreased after subcutaneous inoculation. The cytokines TNF alpha and IFN gamma increased after infection in mice. The subcutaneous model provided scope for improved understanding of the dengue pathogenesis, as well as possible mechanism for protection to subsequent mouse infected by intracranial route in mice. This model could be used to study the vertebrate immune response and evaluation of drugs or vaccine against dengue virus.

关键词： Subcutaneous Murine Model Dengue Disease Immune Competent Mouse Cytokines Innate Immune Response

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Evaluation of Cinnamon by Solid-State NMR Employing Relaxometry

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Materials Sciences and Applications 2023年第3期

作者： pedro Paulo Merat Roberto Cucinelli Neto Vitor santos Ramos Maria Inês Bruno Tavares

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Discrepancies between the responses to skin prick test to food and respiratory antigens in two subtypes of patients with irritable bowel syndrome

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World Journal of Gastroenterology 2008年第19期14卷 3044-3048页

作者： Rosa LS Soares Hamilton N Figueiredo Jose M santos Rita F Oliveira Raquel L Godoy Felipe AP Mendona Internal Medicine Department Faculty of Medicine-Federal Fluminense University Internal Medicine Department Faculty of MedicineFederal Fluminense University Medical school of Universitary Hospital Antonio pedro Federal Fluminense University Medical school of Faculty of Medicine Federal Fluminense University

AIM:To compare the response to skin prick tests (SPTs) to food antigens (FAs) and inhalant allergens (IAs) in patients with two subtypes of irritable bowel syndrome (IBS) and healthy controls. METHODS:We compared the results of SPTs for IAs and FAs in 87 volunteers divided into three groups:diarrhea predominant IBS (D-IBS) GroupⅠ(n = 19), constipation predominant IBS (C-IBS) Group Ⅱ (n = 17), and normal controls Group Ⅲ (n = 51). RESULTS:Of the 285 tests (171 for FAs and 114 for IAs) performed in GroupⅠwe obtained 45 (26.3%) positive responses for FA and 23 (20.1%) for IA. Of the 153 tests for FA in Group Ⅱ, we obtained 66 (20.1%) positive responses, and of the 102 tests for IA, we obtained 20 (19.6%) positive responses. Of the 459 tests for FA performed in Group Ⅲ, we obtained 39 (84%) positive responses, and of the 306 for IA, we obtained 52 (16.9%) positive responses. The numbers of positive responses were not significantly different between the three groups, but in the D-IBS group, the number of SPTFA responses differed significantly from those for the other two groups (P < 0.01). CONCLUSION:Despite the small number of cases studied, the higher reactivity to FAs in GroupⅠcompared to Groups Ⅱ and Ⅲ adds new information, and suggests the presence of a possible alteration in intestinal epithelial function.

关键词： Atopy Constipation Diarrhea Foodintolerance Irritable bowel syndrome Skin prick test

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Thiopurine-methyltransferase variants in inflammatory bowel disease:Prevalence and toxicity in Brazilian patients

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World Journal of Gastroenterology 2014年第12期20卷 3327-3334页

作者： Ana Teresa P Carvalho Barbara C Esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza Disciplina de Gastroenterologia e Endoscopia Digestiva Universidade do Estado do Rio de Janeiro Programa de Carcinogênese Molecular Instituto Nacional de Cancer Laboratório de Toxicologia e Biologia Molecular Universidade do Estado do Rio de Janeiro Servi?o de Gastroenterologia Departamento de Clínica MédicaUniversidade Federal do Rio de Janeiro

AIM: To analyze the prevalence of thiopurine-methyltransferase (TPMT) genotypes and their association with drug toxicity in inflammatory bowel disease (IBD) patients from southeastern Brazil.

关键词： Inflammatory bowel disease Thiopurine-methyl-transferase Azathioprine Drug toxicity Pancreatitis

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Translation, Adaptation and Validation of a Chinese Medicine Questionnaire to Brazilian Portuguese

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Open Journal of Applied Sciences 2021年第10期

作者： Ciro Blujus dos santos Rohde Lukas Blumrich pedro Oliveira Dias Martins Fernanda Babler Wu Tu Hsing Hermano Tavares

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Parasite Fauna of Lutjanus synagris Commercialized in the Fish Market from Bragança-PA,Brazil

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Journal of Zoological Research 2019年第3期1卷 1-4页

作者： Natalino da Costa Sousa Daiana Silva dos santos Silmara Rosa Silva Alexandre Vaz da Silva Jucimauro de Araújo P.Junior João Vitor Antunes Miranda pedro Rodrigo Nery de Souza Matheus Carvalho de Melo Fagner de Melo Xavier Wellington Lima da Silva Junior Fabricio Ramos Menezes Márcia Valeria Silva do Couto Federal University of Pará(UFPA) Campus BragançaAlameda Leandro RibeiroBragança68600-000PABrazil Federal University of Pará(UFPA) Campus Tucuruí-ParáBrazil Federal University of Sergipe(UFS) Departamento de Pesca e Aquicultura.Aracaju-SergipeBrazil Instituto Federal do Pará(IFPA) Campus de Cametá-ParáBrazil

Studies about the parasite fauna of marine fish highlights as an important problem for public health with zoonotic parasites or affecting the fish ***,this study evaluated the parasite fauna of Lutjanus synagris commercialized in the fish market from Braganç*** laboratory,58 fish were measured,weighted and conducted to parasitological analysis to determine parasitological indexes and relative *** parasite was fixed and identified until to the lowest taxonomic *** the parasitological analysis,it found Cymothoidae,Digenea,Cucullanus *** Procamallanus(Spirocamallanus)sp.,with total prevalence 67.24%.Digenea showed the highest prevalence and mean intensity *** nematode,Cucullanus *** the greater prevalence and relative dominance,while Procamallanus(Spirocamallanus)*** the greater mean intensity and *** showed the lowest prevalence and mean intensity *** conclusion,the parasite fauna of *** has been noted with low diversity,reporting the nematode occurrence Procamallanus(Spirocamallanus).

关键词： Cymothoidae Parasite Marine fish

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Are There Schizophrenia Genetic Markers and Mutations? A Systematic Review and Meta-Analyses

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Health 2017年第5期9卷 811-838页

作者： Maria Auxiliadora Brasil Sampaio Cardoso Tárcia Januário do Nascimento Gabriel Pereira Bernardo Lorena Pereira Bernardo Maria Mirelle Ferreira Leite Barbosa pedro Januário Nascimento Neto Danilo Ferreira de Sousa Antonio Gilvan Teixeira Júnior Marcos Antonio Pereira de Lima Marcial Moreno Moreira David de Sousa Gregório Lídia Coelho do Nascimento santos Modesto Leite Rolim Neto Postgraduate Program in Health Science FMABC Santo André Brazil Faculty of Medicine Estacio (FMJ) Juazeiro do Norte Brazil Federal University of Cariri (UFCA) Faculty of Medicine Barbalha Brazil Science without Borders fellow at University of Liverpool Liverpool UK

Background: Schizophrenia is a severe psychiatric disorder with a complex genetic factor determining its disease onset. Nevertheless, it is not clear in this mental disorder. Objective: To conduct a systematic review of articles regarding the genetic markers and mutations in schizophrenia. Methods: A systematic review of articles on genetic markers and mutations in schizophrenia, published from January 1, 2011, to September 7, 2015, on SCOPUS database was carried out. Search terms were “Genetic markers”, “Mutation”, and “Schizophrenia”. Results: Of the 527 retrieved studies, 31 met the eligibility criteria. Genetic polymorphism, Immune-associated genes, TCF4 and ZNF804A association with microRNA, Neuregulin gene, Chromosome 13q32 and 11p15.4, genes involved in glutamatergic via schizophrenia and brain structure, appeared to be associated with the origin of schizophrenia. Conclusion: Some studies show genes involved in several pathways leading to the disease pathogenesis such as that one related with the dopaminergic and immune system, or rare alleles. Some genes present no involvement in the etiology of this mental disorder. These findings clarify the genetic complexity of schizophrenia and affirm that together, the genes have an overall effect greater than the sum of the individual effect of each gene.

关键词： Schizophrenia Genetic Markers Mutations Systematic Review

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Experimental salt cavern in offshore ultra-deep water and well designevaluation for CO_(2) abatement

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International Journal of Mining Science and Technology 2019年第5期29卷 641-656页

作者： Alvaro Maia da Costa pedro V.M.Costa Antonio C.O.Miranda Mariana B.R.Goulart Okhiria D.Udebhulu Nelson F.F.Ebecken Ricardo C.Azevedo Sérgio M.de Eston Giorgio de Tomi Andre B.Mendes Julio R.Meneghini Kazuo Nishimoto Claudio Mueller Sampaio Camila Brandao Alexandre Breda University of Sao Paulo(USP) Sao Paulo 01000-000Brazil Modecom-Technology in Geomechanics and Computing Modeling Rio de Janeiro 27570-000Brazil Department of Civil and Environment Engineering University of Brasília(UnB)Brasília 70000-000Brazil COPPE Federal University of Rio de JaneiroRio de Janeiro 27570-000Brazil Technology Division of Shell Shell BrazilRio de Janeiro 27570-000Brazil

This paper presents a proposal for an experimental salt cavern in offshore ultra-deep water for CO2 abatement,including the instrumentation plan and well conceptual design evaluated for carbon capture and storage(CCS)*** studies are based on applied computational mechanics associated with field experimentation that has contributed to the technical feasibility of the underground potash mine at the State of Sergipe in *** knowhow allowed the stability analysis of several salt caverns for brine production at the State of Alagoas in Brazil and to the drilling through stratified thick layers of salt of the pre-salt reservoirs in santos ***,this knowledge has been applied in the design of onshore and offshore salt caverns opened by dissolution for storage of natural gas and *** geomechanical study,through the application of computational mechanics,of offshore giant salt caverns of 450 m high by 150 m in diameter,shows that one cavern can store about 4 billion Sm3 or 7.2 million tons of *** the construction of the giant cavern,which will be the first gas storage offshore in the world,it has been decided to develop an experimental one,with smaller size,to obtained field *** experimental cavern will allow the calibration of parameters to be used in the structural integrity analysis of the cavern and well for storage of natural gas which is rich in CO2 under high pressure.

关键词： Salt cavern Pre-salt reservoir Geomechanical study Carbon capture and storage

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