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Inheritance of AFLP markers and genetic linkage analysis in two full-sib families of the marine shrimp Litopenaeus vannamei(Crustacea,Decapoda)

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Advances in Bioscience and Biotechnology 2014年第3期5卷 273-281页

作者： Michele Mantovani Goncalves Luciana Correia de Almeida Regitano Cosme Damiao Cruz Caio Cesio Salgado Patricia Domingues de Freitas Joao Luis Rocha Ana Karina Guerrelhas Teixeira pedro manoel galetti-junior Departamento de Genetica e Evolucao Universidade Federal de Sao CarlosSao CarlosBrasil Embrapa Pecuaria Sudeste Sao CarlosSPBrasil Departamento de Biologia Geral Universidade Federal de VicosaVicosaBrasil Genearch Aquacultura Ltda Rua Pedro Zuca s/nPraia de PititingaRio do FogoRNBrasil

The cultivation of the marine species of shrimp Litopenaeus vannamei has emerged as one of the segments that best develop in the Brazilian aquaculture, representing the agribusiness that grew the most in recent years. Regarding the economic importance of the farming of this species in Brazil, further studies on genetic improvement were conducted. A F2 segregating population consisting of 192 samples for each G1 and G2 families, from crossing inbred lines was used for the studies performed in the present work. The genetic linkage analysis was based on polymorphic markers derived from nine AFLP (Amplified Fragment Length Polymorphism) primers. Fourteen genetic linkage groups including 103 segregating polymorphic markers were constructed covering350 cMfor G1 and four genetic linkage groups including 59 markers were constructed covering300 cMfor G2. Simple marker analyses were performed among individuals evaluated phenotypically, finding markers linked to genes that may be potentially important and useful to assess characteristics of economic importance for traits related to weight and the disease IMN (idiopathic muscle necrosis). The statistical model including markers explained major proportion of phenotypic characteristic weight in relation to disease incidence IMN.

关键词： Shrimp Litopenaeus vannamei AFLP Genetic Linkage Groups

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Ecophysiology and multivariate analysis for production of Tachigali vulgaris in Brazil:Influence of rainfall seasonality and fertilization

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林业研究:英文版 2023年第5期34卷 1289-1305页

作者： pedro Henrique Oliveira Simoes Candido Ferreira de Oliveira Neto manoel Tavares de Paula Dênmora Gomes de Araújo Rodrigo Silva do Vale Joao Olegário Pereira de Carvalho Universidade Federal de Mato Grosso Campus Universitario de SinopSinop 78550728Mato GrossoBrazil Universidade Federal Rural da Amazonia Campus BelemBelem 66077830ParaBrazil Universidade do Estado do Para Centro de Ciencias Naturais e TecnologiaBelem 66095015ParaBrazil Universidade Federal Rural da Amazonia Campus Capitao-PocoCapitao Poco 68650000ParaBrazil

Studies on fertilization management of species native to the Amazon for energy plantations contribute to the diversity of species use and reduce biological risk due to the excessive use of clones or hybrids of *** study evaluates the effect of precipitation seasonality and phosphorus and potassium fertilization on gas exchange in a Tachigali vulgaris *** levels of P(zero,65.2,130.4 kg ha^(-1))and three of K(zero,100.0,200.0 kg ha^(-1))were applied in a 3×3 factorial randomized block *** exchange measurements were conducted in April and November *** low rainfall,high irradiance period,photo synthetic rates were up to four times higher than in the high rainfall period,reaching 20.3μmol m^(-2)s^(-1)in the treatment with 130.4 g kg^(-1)of P and 100.0 g kg^(-1)of *** analysis and principal component analysis reduced the initial eight gas exchange variables to two and three principal components in periods of high and low rainfall,*** multivariate method used in this study readily identified variations in the variables as a function of rainfall,with high reliability in explaining the data set.

关键词： Photosynthesis rate Stomatal conductance Principal component analysis Factor analysis Tachigali vulgaris

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Physicians and Conflicts of Interest

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International Journal of Clinical Medicine 2014年第16期5卷 956-965页

作者： Deborah Pimentel Maria-Jesia Vieira Joao pedro Declerc FSNeves manoel Juvenal da Costa-Neto Lais Costa Souza Oliveira Department of Medicine Universidade Federal de SergipeAracajuBrazil Universidade Tiradentes AracajuBrazil Postgraduate Program in Health Sciences Universidade Federal de SergipeAracajuBrazil Universidade Federal de Sergipe AracajuBrazil

In medical practice, the most commonly observed conflicts of interest are the ones existing in the relations between professionals and industries linked to health or between the former and health services. These relations are no longer a partnership in search of the best to be offered to patients and tend to take up a commercial role, in a seduction game where, each one, in their own manner, seeks benefits. The purpose of this study is to analyze the existence of these conflicts of interest, in an exploratory and qualitative study, with physician focal groups. The results were grouped in two categories: benefits received by physicians and the justifications provided by them for the establishment of these partnerships. The most common benefits are: percentages over orthoses and prosthetics received by the industry;discounts and credits on rent or condominium fees for exam indications and procedures;rewards offered by the pharmaceutical industry for the indication of their products. Physicians have pointed out as main determinants for ethical missteps that involve conflicts of interest, the character and family formation as well as the lack of denunciations. The fact that efficient and widespread laws for resolution of conflicts of interest do not exist makes it necessary to recognize their existence with mechanisms of management and transparency, perhaps as the only way to avoid negative interference in physician behavior.

关键词： Ethics Physicians Conflicts of Interest

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Development and implementation of an automated system to aid laboratory diagnosis using image processing

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Journal of Biomedical Science and Engineering 2013年第5期6卷 579-585页

作者： Alvaro manoel de Souza Soares Marco Rogério da Silva Richetto Joao Bosco Goncalves pedro Paulo Leite do Prado Department of Mechanical Engineering University of TaubateTaubateBrasil Department of Electrical Engineering University of TaubateTaubateBrasil

The objective of this work is to provide an automatic system to count white blood cells in a blood smear. To do so an experiment was assembled, composed by a standard microscope with two step motors coupled to its knobs in order to move the microscope in x and y directions and a web cam which was mounted in the top of the microscope responsible for to acquire images from the smear. The step motors and the web cam are controlled by a microcomputer PC standard via software developed inDelphi. The motors use the parallel port to communicate with the PC and the camera use the USB port. The main idea is to set an initial point into the smear and the automated system will carry over the smear acquiring images (frames with 640 × 480 pixels) and counting the white blood cells encountered. The double histogram threshold technique is implemented to initially exclude the red cells from the image leaving only the white ones. Preliminaries results are obtained and show that the system is quite fast and has a good capacity of selection, even when different kinds of smear are used.

关键词： Image Processing Robotics Automation Pattern Recognition

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Modeled Surface Observations for Spatial Analysis of Landscape Dynamics

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Journal of Geographic Information System 2013年第4期5卷 409-417页

作者： manoel do Couto Fernandes Tata Lacale Canal dos Santos pedro Henrique Ferreira Coura Paulo Marcio Leal de Menezes Alan Jose Salomao Graca Department of Geography Federal University of Rio de JaneiroRio de JaneiroBrazil

Landscape dynamics is a geoecological characteristic that defines changes in a landscape spatial structure and function during an established time scale. In this kind of analysis, geoprocessing is an essential tool, because it combines several technologies that help in this work. However, the majority of landscape dynamic investigations do not consider the dimensionality of the data and the information to be used, which is projected not measured from modeled (real) surface observations, and presents underestimated results, mainly in irregular relief landscapes. Considering that, this paper intends to assess the difference between observations on modeled and planimetric surfaces on the interpretation of landscape dynamics. The study was conducted in the massif of Tijuca, Rio de Janeiro (Brazil) using land use and soil cover maps of different times and a digital elevation models (DEM) generated based on a triangular irregular. The results show an increase of dynamic areas values when observations were carried out on modeled surface, as well as the deforestation rate (17.57% or 0.13 km2/year). This survey showed higher values in comparison with observations on planimetric surface, characterizing more realistic interpretations of how the structural elements and analyses made from these are structured in the landscape.

关键词： DEM Modeled Surface Landscape Change

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Antifungal Susceptibility Profile <i>In Vitro</i>Fungal Air in a Hospital Environment

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Open Journal of Medical Microbiology 2018年第3期8卷 35-46页

作者： Otávio Vilela de Figueiredo Luiz Francisley de Paiva Matheus Valejo Peixoto pedro Guilherme Paula Bariani Thiago Silva Pinto manoel Araujo Teixeira Ana Beatriz Alkmim Teixeira-Loyola School of Medicine University of the Sapucai Valley Pouso Alegr Minas Gerais Brazil Research Laboratory University of the Sapucai Valley Pouso Alegre Minas Gerais Brazil Department of Biology University of the Sapucai Valley Pouso Alegre Minas Gerais Brazil Department of Microbiology University of the Sapucai Valley Pouso Alegre Minas Gerais Brazil

Purpose: The aim of this study was to isolate and identify potentially pathogenic airborne fungi from Hospital das Clínicas Samuel Libanio in the city of Pouso Alegre-MG and evaluate their susceptibility to natural and industrial products. Methods: The air collection was performed by passive sedimentation in the morning during the autumn and winter seasons. Petri dishes were open as the location of air conditioning. The isolates were subjected to pathogenicity test. The identification of the fungi was performed according to the macroscopic evaluation and micromorphology. Potentially pathogenic isolates were susceptibility tested by disc diffusion method. The agents used were insecticides and industrial cleaning products and essential oils of citronella plants, lemon grass, eucalyptus and Melaleuca extracted by steam distillation method. Results: We obtained a total of 356 fungal isolates. The inside door environments were 126 (35.39%) and the outside environments were 230 (64.6%) isolates. The 22 (6.18%) isolates from the inside and 25 (6.18%) outside the hospital showed pathogenic potential. This isolates were identified as Acremonium spp., A. niger, A. terreus, A. versicolor, Curvularia sp., Penicillium sp. and Scopulariopsis sp. Susceptibility testing it was observed that most of the isolates were susceptible to the principle product containing sodium hypochlorite. Citronella oil showed enormous potential inhibition against all isolates. Already lemon grass oil was effective only against isolates of Penicillium spp. Conclusions: All genres identified are significant allergens, which can cause respiratory disease in both immunocompromised individuals such as asthmatics and people with any immune deficit. The monitoring of environmental sources should be performed, especially in special areas with immunocompromised patients. Despite efforts to try to reduce fungal infections hospital there are still flaws in the strategies employed.

关键词： Fungi Air Conditioning Housekeeping Hospital Disk Diffusion Antimicrobial Tests

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Unravelling the protracted U-Pb zircon geochronological record of high to ultrahigh temperature metamorphic rocks:Implications for provenance investigations

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Geoscience Frontiers 2023年第2期14卷 116-132页

作者： Mahyra Tedeschi pedro Leonardo Rossi Vieira Matheus Kuchenbecker Bruno VRibeiro Vitor Barrote Humberto Reis Laura Stutenbecker Cristiano Lana Antonio pedrosa-Soares Ivo Dussin Programa de Pós-graduação em Geologia da Universidade Federal de Minerais Centro de Pesquisas Manoel Teixeira da CostaInstitute of GeosciencesFederal University of Minas GeraisAv.Antônio Carlos6627Belo Horizonte 31270-901Brazil Institute of Geological Sciences University of Bern3012 BernSwitzerland Laboratório de Estudos Tectônicos Universidade Federal dos Vales do Jequitinhonha e MucuriRodovia MGT 367Km 583-Diamantina 39100-000Brazil School of Earth Atmosphere and EnvironmentMonash UniversityClaytonVictoria 3800Australia Timescales of Mineral Systems Group School of Earth and Planetary SciencesCurtin UniversityPerthWA 6845Australia Freie Universität Berlin Institut für Geologische WissenschaftenGeochemieMalteserstr.1074-10012249 BerlinGermany Departamento de Geologia-Escola de Minas Universidade Federal de Ouro Preto 35400-000Brazil Department of Materials and Earth Sciences Institute of Applied GeosciencesTU DarmstadtSchnittspahnstr.964287 DarmstadtGermany TEKTOS Geotectonic Research Group Geology InstituteRio de Janeiro State UniversitySão Francisco Xavier Street524/4030-AMaracanãRio de Janeiro 20550-900Brazil

The assessment of detrital zircon age records is a key method in basin analysis,but it is prone to several biases that may compromise accurate sedimentary provenance *** to ultrahigh temperature(HT-UHT)metamorphism(especially if T>850℃)is herein presented as a natural cause of bias in provenance studies based on U-Pb detrital zircon ages,since zircon from rocks submitted to these extreme and often prolonged conditions frequently yield protracted,apparently concordant,geochronological *** age spreading can result from disturbance of the primary U-Pb zircon system,likewise from(re)crystallization processes during multiple and/or prolonged metamorphic *** this contribution,available geochronological data on Archean,Neoproterozoic and Palaeozoic HT-UHT metamorphic rocks,acquired by different techniques(SIMS and LA-ICP-MS)and showing distinct compositions,are reassessed to demonstrate HT-UHT metamorphism may result in modes and age distributions of unclear geological *** a consequence,it may induce misinterpretations on UPb detrital zircon provenance analyses,particularly in sedimentary rocks metamorphosed under such extreme temperature *** evaluate the presence of HT-UHT metamorphism-related bias in the detrital zircon record,we suggest a workflow for data acquisition and interpretation,combining a multi-proxy approach with:(i)in situ U-Pb dating coupled with Hf analyses to retrieve the isotopic composition of the sources,and(ii)the integration of a petrochronological investigation to typify fingerprints of the HT-UHT metamorphic *** proposed workflow is validated in the investigation of one theoretical and one natural example allowing a better characterization of the sedimentary sources,maximum depositional ages,and the tectonic setting of the *** workflow allows to the appraisal of biases imposed by HT-UHT metamorphism and resulting disturbances in the U-Pb detrital zircon record,particularly for sedimentary

关键词： Detrital zircon Protracted zircon record Sedimentary basins UHT metamorphism Prolonged metamorphism Bias in detrital zircon

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Dynamic Modeling and Experimental Damping Identification of a Flexible Structure

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材料科学与工程（中英文A版） 2013年第7期3卷 505-509页

作者： Marcelo Rodolfo de Assis Alvaro manoel de Souza Soares JoAo Bosco Goncalves pedro Paulo Leite do Prado Mechanieal Engineering Department University of Taubate Campus da Juta Taubatd 12.060-440 SP Brazil Eletrical Engineering Department University of Taubatd Campus da Juta Taubatd 12.060-440 SP Brazil

This paper address the modeling and experimental damping of a cantilevered beam. Its objectives are to describe the design and the implementation of an experiment to study the dynamics and the experimental identification of the beam’s damping matrix. To perform the procedure an experimental setup was assembled composed by a cantilevered beam an impact hammer and an accelerometer. The dynamic modeling and an experimental modal analysis identification in the frequency domain was applied to obtain the vibrations modes of the beam and a procedure to the identification of the system damping matrix based on genetic algorithms method’s was used do complete the model. The results are quite good and show a good agreement between the real and experimental systems.

关键词：实验鉴定阻尼识别动态建模柔性结构实验模态分析阻尼矩阵悬臂梁遗传算法

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Physicians and Conflicts of Interest

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International Journal of Clinical Medicine（临床医学国际期刊） 2014年第16期

作者： Deborah Pimentel Maria-Jésia Vieira Jo？o pedro Declerc F. S. Neves manoel Juvenal da Costa-Neto Laís Costa Souza Oliveira

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Ecophysiology and multivariate analysis for production of Tachigali vulgaris in Brazil:Influence of rainfall seasonality and fertilization

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林业研究（英文版） 2023年第5期

作者： pedro Henrique Oliveira Sim?es Candido Ferreira de Oliveira Neto manoel Tavares de Paula Dênmora Gomes de Araújo Rodrigo Silva do Vale Jo?o Olegário Pereira de Carvalho

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