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Application of a Pulsed Electric Field to Modify the Free Energy of the Active Site of an Azoreductase

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Journal of Applied Mathematics and Physics 2024年第1期12卷 249-255页

作者： Jesus Magyber de Jesus Miranda pedro guillermo reyes-Romero Jorge Mulia-Rodríguez Daniel Osorio-González Molecular Biophysics Laboratory Faculty of Sciences Autonomous University of State of Mexico Toluca Mexico Advanced Physics Laboratory Faculty of Sciences Autonomous University of State of Mexico Toluca Mexico

The Gibbs free energy is strongly related to the stability and catalytic function of an enzyme through the energetic changes that occur in the chemical reactions the enzyme catalyzes. In this in silico study, a pulsed electric field was applied to an azoreductase, and its effect on the Gibbs free energy of molecular docking with two dyes was measured. We propose that certain stimuli from a pulsed electric field favor the structural stability of the enzyme by promoting an arrangement in the active site, potentially leading to an enhancement of enzymatic activity overall.

关键词： Pulsed Electric Field Molecular Dynamics Docking Azoreductase

Human Prion Protein Conformational Changes Susceptibility: A Molecular Dynamics Simulation Study

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Open Journal of Biophysics 2014年第4期4卷 169-175页

作者： Laura Alejandra Mandujano-Rosas Daniel Osorio-González pedro guillermo reyes-Romero Jorge Mulia-Rodríguez Laboratory of Molecular Biophysics Faculty of Sciences Autonomous University of State of Mexico Toluca Mexico Laboratory of Advaced Physics Faculty of Sciences Autonomous University of State of Mexico Toluca Mexico

Prion proteins are related to the development of incurable and invariably fatal neurodegenerative diseases in humans and animals. The pathogenicity involves the conversion of the host-encoded-alpha rich isoform of prion protein, PrPC, into a misfolded beta-strand rich conformer, PrPSc. Although it has already been described that many punctual mutations alter the stability of PrPC, making it more prone to adopt an abnormal misfolded structure, the majority of cases reported among general population are sporadic in wild-type organisms. Thus, in this work we studied the dynamics and stability profiles of wild-type human prion protein by Molecular Dynamics (MD) simulation at different solvent temperatures. This analysis brought out certain residues and segments of the prion protein as critical to conformational changes;these results are consistent with experimental reports showing that protein mutants in those positions are related to the development of disease.

关键词： Prion Protein Misfolding Susceptibility Molecular Dynamics Simulation

A Study of the Structural, Optical and Electrical Properties of SnS Thin Films Modified by Plasma

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材料科学与工程（中英文B版） 2013年第6期3卷 352-358页

作者： Aar6n Gomez Horacio Martinez Manuela Calixto-Rodriguez David Avellaneda pedro guillermo reyes Osvaldo Flores Laboratorio de Fisica Avanzada Facultad de Ciencias Universidad Autonoma del Estado de Mexico Estado de Mexico Mexico Laboratorio de Espectroscopia Instituto de Ciencias Fisicas Universidad Nacional Aut6noma de Mdxico Cuernavaca MorelosMdxico Intituto de Energia Renovable Universidad Nacional Aut6noma de Mdxico Cuernavaca Morelos M~xico Facultad de Ingenieria Meconica y Electrica Universidad Autonoma de Nuevo Leon Mexico

Pacing profiles and tactical behaviors of elite runners

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Journal of Sport and Health Science 2021年第5期10卷 537-549页

作者： Arturo Casado Brian Hanley pedro Jimenez-reyes Andrew Renfree Faculty of Health Sciences Isabel I UniversityBurgos 09003Spain Carnegie School of Sports Leeds Beckett UniversityLeeds LS63QSUK Centre for Sport Studies Rey Juan Carlos UniversityMadrid 28943Spain Institute of Sport and Exercise Science University of WorcesterWorcester WR26AJUK

The pacing behaviors used by elite athletes differ among individual sports,necessitating the study of sport-specific pacing ***,pacing behaviors adopted by elite runners differ depending on race ***“all-out”strategy,characterized by initial rapid acceleration and reduction in speed in the later stages,is observed during 100 m and 200 m events;400 m runners also display positive pacing patterns,which is characterized by a reduction in speed throughout the ***,800 m runners typically adopt a positive pacing strategy during paced“meet”***,during championship races,depending on the tactical approaches used by dominant athletes,pacing can be either positive or negative(characterized by an increase in speed throughout).A U-shaped pacing strategy(characterized by a faster start and end than during the middle part of the race)is evident during world record performances at meet races in 1500 m,5000 m,and 10,000 m *** a parabolic J-shaped pacing profile(in which the start is faster than the middle part of the race but is slower than the endspurt)can be observed during championship 1500 m races,a negative pacing strategy with microvariations of pace is adopted by 5000 m and 10,000 m runners in championship *** cross country and marathon championship races are characterized by a positive pacing strategy;whereas a U-shaped pacing strategy,which is the result of a fast endspurt,is adopted by 3000 m steeplechasers and half *** contrast,recent world record marathon performances have been characterized by even pacing,which emphasizes the differences between championship and meet races at distances longer than 800 *** reviewed suggest further recommendations for *** the whole race,800 m runners should avoid running wide on the *** turn,during major championship events,1500 m,5000 m,and 10,000 m runners should try to run close to the inside of the track as much as possible during t

关键词： Athletics Distance running Pacing Sprinting

Quantitative morphometric analysis of the Jama River profile in a tectonically active margin (Northwestern Ecuador)

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Journal of Mountain Science 2018年第5期15卷 966-975页

作者： pedro S.B. reyes Mauricio E. VALAREZO Jenry CORDOVA Francois A. MICHAUD Camilo ZAPATA Department of Geology Escuela Politécnica Nacional Ladrón de Gueuara E11-253 PO.Box 17-01-2759 Quito Ecuador C-eostrat S.A. Jorge Drom N39-188y Gaspar de Villaroel Quito Ecuador Department of Civil and Environmental Engineering Escuela Politécnica National Ladrón de Guevara E11-253 PO.Box17-ol-2759 Quito Ecuador Sorbonne Université Université Cote d'Azur IRD CNRS Observatoire de la Cdte d'Azur C-eoazur 250 rue A. EinsteinSophia Antipolis 06560 Valbonne France

The longitudinal profile of a river channel can be described in function of mathematical expressions. The logarithmic fit is the most used method to describe the relative equilibrium of a channel elevation profile. Rivers showing zones of high channel gradient and convex-upward profiles can be evaluated in function of the offset distance with respect to the logarithmic curve. The Jama River profile has been constructed using differential GPS data for the downstream reach and 4-m-grid DEM data base for the remaining headward profile. The resulting longitudinal profile shows a prominent knickpoint of about 80 m in elevation characterized by sharp local convexity. The offset distance in vertical direction from the logarithmic plot is interpreted as the successive accumulation of multiple uplift episodes associated with the Jama Fault System activity. The horizontal offset is suggested to represent the remaining retreated distance for the upstream propagation of the Jama knickpoint. The highly resistant Cretaceous rocks outcropping along the Jama knickpoint reach acts as an inflection point between two subprofiles that show well-fitted logarithmic curves, thereby representing a temporary partitioned equilibrium along the Jama River profile.

关键词： Morphometry Jama River Stream profile Active tectonics

CONSTANS alters the circadian clock in Arabidopsis thaliana

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Molecular Plant 2024年第8期17卷 1204-1220页

作者： pedro de los reyes Gloria Serrano-Bueno Francisco J.Romero-Campero He Gao Jose M.Romero Federico Valverde Plant Development Group-Institute for Plant Biochemistry and Photosynthesis Consejo Superior de Investigaciones CientificasUniversidad de SevillaSevilleSpain Department of Computer Science and Artificial Intelligence Universidad de SevillaSevilleSpain Department of Plant Developmental Biology Max Planck Institute for Plant Breeding ResearchCologneGermany Department of Plant Biochemistry and Molecular Biology Universidad de SevillaSevilleSpain

Plants are sessile organisms that have acquired highly plastic developmental strategies to adapt to the *** these processes,the floral transition is essential to ensure reproductive success and is finelyregulated by several internal andexternal genetic *** photoperiodic pathway,which controls plant response to day length,is one of the most important pathways controlling *** Arabidopsis photoperiodic flowering,CONSTANS(CO)is the central gene activating the expression of the florigen FLOWERING LOCUS T(FT)in the leaves at the end of a long *** circadian clock strongly regulates Co ***,to date,no evidence has been reported regarding a feedbackloop from the photoperiod pathway back to the circadian *** transcriptional networks,we have identified relevant network motifs regulating the interplay between the circadian clock and the photoperiod *** expression,chromatin immunoprecipitation experiments,and phenotypic analysis allowed us to elucidate the role of Co over the circadian *** with altered cO expression showed a different internal clock period,measured by daily leaf rhythmic *** showed that co upregulates the expression of key genes related to the circadian clock,such as CCA1,LHY,PRR5,and Gl,at the end of a long day by bindingto specific sites on their ***,a high numberof PRR5-repressed target genes are upregulated by CO,and this could explain the phase transition promoted by *** CO-PRR5 complex interacts with the bZiP transcriptionfactor HY5andhelps to localize the complex in the promoters of clock *** together,our results indicate that there may be a feedback loop in which co communicates back to the circadian clock,providing seasonal information to the circadian system.

关键词： CONSTANS circadian clock photoperiod

Regulation of floral senescence in Arabidopsis by coordinated action of CONSTANS and jasmonate signaling

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Molecular Plant 2022年第11期15卷 1710-1724页

作者： Gloria Serrano-Bueno pedro de los reyes Andrea Chini Gabriel Ferreras-Garrucho Víctor Sánchez de Medina-Hernández Marta Boter Roberto Solano Federico Valverde Plant Development Group Institute for Plant Biochemistry and PhotosynthesisCSIC-Universidad de Sevilla41092 SevillaSpain Department of Plant Molecular Genetics Centro Nacional de BiotecnologíaCSIC28049 MadridSpain Crop Science Centre University of CambridgeCambridge CB3 OLE CambridgeUK Gregor Mendel Institute(GMI) Austrian Academy of SciencesVienna BioCenter(VBC)ViennaAustria

In Arabidopsis,photoperiodic flowering is controlled by the regulatory hub gene CONSTANS(CO),whereas floral organ senescence is regulated by the jasmonates(JAs).Because these processes are chronologically ordered,it remains unknown whether there are common regulators of both *** this study,we discovered that CO protein accumulates in Arabidopsis flowers after floral induction,and it displays a diurnal pattern in floral organs different from that in the *** observed that altered CO expression could affect flower senescence and abscission by interfering with JA response,as shown by petal-specific transcriptomic analysis as well as CO overexpression in JA synthesis and signaling *** found that CO has a ZIM(ZINC-FINGER INFLORESCENCE MERISTEM)like domain that mediates its interaction with the JA response repressor JAZ3(jasmonate ZIM-domain 3).Their interaction inhibits the repressor activity of JAZ3,resulting in activation of downstream transcription factors involved in promoting flower ***,we showed that CO,JAZ3,and the E3 ubiquitin ligase COI1(Coronatine Insensitive 1)could form a protein complex in planta,which promotes the degradation of both CO and JAZ3 in the presence of *** together,our results indicate that CO,a key regulator of photoperiodic flowering,is also involved in promoting flower senescence and abscission by augmenting JA signaling and *** propose that coordinated recruitment of photoperiodic and JA signaling pathways could be an efficient way for plants to chronologically order floral processes and ensure the success of offspring production.

关键词： CONSTANS flower senescence jasmonic acid

Integrative Analysis of Transcriptome and Phenolic Compounds Profile Provides Insights into the Quality of Soursop (Annona muricata L.) Fruit

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Phyton-International Journal of Experimental Botany 2024年第7期93卷 1717-1732页

作者： Yolotzin Apatzingán Palomino-Hermosillo Ángel Elpidio Díaz-Jasso Rosendo Balois-Morales Verónica AlhelíOchoa-Jiménez pedro Ulises Bautista-Rosales guillermo Berumen-Varela Unidad de Tecnología de Alimentos-Secretaría de Investigación y Posgrado Universidad Autónoma de Nayarit.Ciudad de la Cultura SNTepic63000NayaritMéxico Programa Académico de Biología Unidad Académica de AgriculturaUniversidad Autónoma de NayaritKm.9.Carretera Tepic-CompostelaXalisco63780NayaritMéxico Estancias Posdoctorales-Consejo Nacional de Humanidades Ciencias y TecnologíasCoordinación de Apoyos a Becarios e InvestigadoresDirección de PosgradoCiudad de México03940México

Soursop(Annona muricata L.)is a tropical fruit highly valued for its uniqueﬂavor,nutritional value,and health-promoting *** ripening process of soursop involves complex changes in gene expression and metabo-lite accumulation,which have been studied using various omics *** analysis has provided insights into the regulation of key genes involved in ripening,while metabolic compound analysis has revealed the presence of numerous bioactive compounds with potential health beneﬁ***,the integration of transcrip-tome and metabolite compound data has not been extensively explored in ***,in this paper,we present a comprehensive analysis of the transcriptome and phenolic compound proﬁles of soursop during *** integration analysis showed that the genes and phenolic compounds were mainly involved in the starch and sucrose metabolism pathways during soursop ***,the phenolic compounds Kaempferol 3-Q-galactoside,Procyanidin C1,Procyanidin trimmer C1,and m-Coumaric,as well as the genes Ubiquitin-like protein 5(UBL5_ARATH),ATP-dependent zinc metalloprotease FTSH8(FTSH8_ORYSJ),Zinc transporter 4(ZIP4_AR-ATH),Thioredoxin-like 3-1(TRL31_ORYSJ),Mitogen-activated protein kinase YODA(YODA_ARATH),R-man-delonitrile lyase-like(MGL_ARATH),26s protease regulatory subunit 6A homolog(PRS6_SOLLC),Cytochrome P45072A13(C7A13ARATH),Cytochrome P45084A1(C84A1_ARATH)and Homoserine O-trans-acetylase(MET2-ORYSJ)were correlated and differentially accumulated and expressed,*** study provides new insights into the molecular mechanisms underlying soursop ripening and may contribute to the development of strategies for improving the nutritional quality and shelf life of this important fruit.

关键词： Bioinformatics data integration genes metabolites postharvest storage ripening

Dietary Monosodium Glutamate Does Not Affect the Electrocardiographic Profiles of Diabetic and Nondiabetic Wistar Rats

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Food and Nutrition Sciences 2019年第6期10卷 613-625页

作者： Miguel Arcanjo Areas Hellen Dea Barros Maluly Jean Franciesco Vettorazzi Everardo Magalhaes Carneiro Felix guillermo reyes reyes Institute of Biology University of Campinas/UNICAMPCampinasBrazil School of Food Engineering University of Campinas/UNICAMPCampinasBrazil

Background and Aims: Some studies have recently indicated that dietary monosodium glutamate (MSG) is linked to obesity and the development of diabetes. Both diseases induce cardiovascular changes, such as increases in blood pressure and arrhythmias, including ventricular fibrillation, which may result in sudden death. Here, we aimed to investigate the effects of oral MSG administration on the electrical conduction and histological dysfunctions of the heart in control and diabetic Wistar rats. Methods and Results: Twenty-one-day-old Wistar rats were fed diets containing 0.0%, 1.0%, 2.5% or 5.0% MSG for 70 days. After this period, diabetes was induced with streptozotocin (STZ;50 mg/kg bw) in half the rats and after an additional 21 days period;the electrocardiographic parameters and heart histology were evaluated. Diabetic rats demonstrated a reduction in heart rate as well as an enlargement of the QRS complex and QT and QTc intervals. Nevertheless, those changes are typical of STZ-induced diabetes, mainly because of electrolyte disturbances. The presence of MSG in the diet did not change the parameters evaluated between the group that received MSG and the group that did not receive MSG. Moreover, no histological alterations in the heart were observed due to MSG ingestion. Conclusion: Based on this evidence, diets containing MSG did not interfere with cardiovascular changes due to diabetes;there were no differences in the electrocardiographic and histological characteristics of the hearts of rats treated with MSG.

关键词： Monosodium Glutamate Diabetes Cardiovascular Changes Streptozotocin