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Morphological and Molecular Identification of Fungi Associated with Sesame Diseased Plants of the Three Agroclimatic Zones of Burkina Faso

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American Journal of Plant Sciences 2023年第3期14卷 290-307页

作者： Wendolian Romain Soalla pawindé elisabeth zida Bouma James Neya Kadidia Koita Laboratoire de Phytopathologie Institut de l’Environnement et de Recherches Agricoles (INERA) Ouagadougou Burkina Faso Laboratoire Biosciences Université Joseph Ki-ZERBO Ouagadougou Burkina Faso

Sesame is Burkina Faso’s second essential agricultural export after cotton. It’s consequently a supply of income for producers and foreign exchange for the country. However, sesame production is characterized by low average yields of about 538 kg·ha-1 at the farmer’s field as compared to the potential yield of the improved varieties (1500 - 2000 kg·ha-1). Fungal diseases are some of the major constraints to sesame production in Burkina Faso. The present study contributes to the development of means to control pathogenic fungi of this crop, which are responsible for significant losses. The objective is to identify the fungi associated with diseased sesame plant samples. To this end, 149 samples of diseased sesame plants were collected from different production sites located in three agro-climatic zones of the country. The analysis of the samples according to the blotting paper method, based on the morphological characteristics of the fungi, allowed the identification of 18 genera with prevalence rates from 2.68% to 97.98%. The most frequently identified genera were Macrophomina (97.98%), Cercospora (86.57%), Fusarium (85.23%), Phoma (62.41%) and Colletotrichum (61.07%). The results also showed a variable distribution of fungi according to the agro-climatic zone with the predominance of Macrophomina in all three zones. Molecular identification by DNA sequencing of 120 isolates belonging to the different fungi detected allowed the identification of 25 species of which the most representative were Macrophomina phaseolina, Cercospora sesami, Corynespora cassiicola, Alternaria simsimi, Alternaria porri, Fusarium oxysporum, F. fujikuroi, F. equiseti, Colletotrichum capsici, and C. gloesporiodes. The present study showed that diseased sesame plants collected from different production sites in Burkina Faso housed several species of fungi. The fungi presence in diseased plants indicates the need to inform and raise the stakeholders’ awareness about the p

关键词： Burkina Faso Fungi Molecular Identification Morphological Identification Sesame

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Morphological Identification of the Main Fungi Associated with Sesame in Burkina Faso

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American Journal of Plant Sciences 2023年第8期14卷 882-895页

作者： Dianyagou Paul Ouali pawindé elisabeth zida Wendolian Romain Soalla Kuilpoko Marie Laure Guissou Laboratoire Sciences de la Vie et de la Terre (LASVT) Université Norbert Zongo Koudougou Burkina Faso Laboratoire de Phytopathologie et de Biotechnologie Végétale Institut de l’Environnement et de Recherches Agricoles/CNRST Ouagadougou Burkina Faso

Several fungal species are responsible for diseases that damage sesame crop and cause production losses. These seed-borne fungi cause serious damage by reducing seed germination. To identify the fungi associated with sesame seeds in Burkina Faso, 72 sesame seed samples were collected from 24 localities in the main production areas (Sudanian zone, Sudano-Sahelian zone, Sahelian zone). These samples were submitted for seed health analysis using the blotting paper incubation method. The results obtained revealed that the seed samples were contaminated by various fungi, the main ones being: Fusarium spp. Link: Fr. infecting 97.22% of the samples, Macrophomina phaseolina (Tassi) Goid. (90.28%), Alternaria sesamicola E. Kawamura (83.33%), Curvularia lunata (Wakker) Boedijn (76.39%), Cercospora sesami A. Zimmerm. (73.61%), Phoma sorghina Boerema, Dorenbosch, & Van Kesteren (73.61%), Aspergillus flavus Link: Fr. (69.44%), Cladosporium sphaerospermum Penz. (56.94%), Rhizopus sp. Ehrenb. (40.28%) and A. niger Tiegh. (36.11%). In each seed sample, 0.25% to 94% of the seeds host one or more fungal species. Regarding the climatic zones, the results revealed that A. sesamicola and A. flavus were more represented in the Sudanian zone than in the Sudano-Sahelian and Sahelian zones;while P. sorghina, and C. sphaerospermum were more encountered in the Sahelian zone than in the other two zones. This study showed that the majority of sesame seeds produced and used by farmers in Burkina Faso host one or more species of fungi at high levels.

关键词： Sesame Fungi Associated with Seeds Seed Health Analysis Burkina Faso

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Morphological and Molecular Identification of Fungi Associated with Sesame Diseased Plants of the Three Agroclimatic Zones of Burkina Faso

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American Journal of Plant Sciences（美国植物学期刊） 2023年第3期

作者： Wendolian Romain Soalla pawindé elisabeth zida Bouma James Neya Kadidia Koita

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Fluid-rock interaction experiments with andesite at 100℃ for potential carbon storage in geothermal reservoirs

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Deep Underground Science and Engineering 2024年第3期3卷 369-382页

作者： Grace E.Belshaw elisabeth Steer Yukun Ji Herwin Azis Benyamin Sapiie Bagus Muljadi Veerle Vandeginste School of Chemistry University of NottinghamNottinghamUK GeoEnergy Research Centre University of NottinghamNottinghamUK British Geological Survey KeyworthUK State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering China University of Mining and TechnologyXuzhouChina PT Supreme Energy JakartaIndonesia Faculty of Earth Sciences and Technology Institut Teknologi BandungBandungIndonesia Faculty of Engineering University of NottinghamNottinghamUK Department of Materials Engineering KU LeuvenCampus BrugesBrugesBelgium

Geothermal energy extraction often results in the release of naturally occurring carbon dioxide(CO_(2))as a *** on carbon storage using volcanic rock types other than basalt under both acidic and elevated temperature conditions has been limited so *** study uses batch reactor experiments at 100℃ to investigate the dissolution of andesite rock samples obtained from an active geothermal reservoir in Sumatra(Indonesia).The samples are subjected to reactions with neutral-pH fluids and acidic fluids,mimicking the geochemical responses upon reinjection of geothermal fluids,either without or with dissolved acidic gases,*** elemental analysis reveals the release of Ca^(2+)ions into the fluids through the dissolution of *** overall dissolution rate of the rock samples is 2.4×10^(–11)to 4.2×10^(–11)mol/(m^(2)·s),based on the Si release during the initial 7 h of the *** dissolution rates are about two orders of magnitude lower than those reported for basaltic rocks under similar reaction *** study offers valuable insights into the potential utilization of andesite reservoirs for effective CO_(2) storage via mineralization.

关键词： andesite carbon sequestration geothermal reservoirs plagioclase dissolution

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Rumen microbial degradation of bromoform from red seaweed(Asparagopsis taxiformis)and the impact on rumen fermentation and methanogenic archaea

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Journal of Animal Science and Biotechnology 2024年第1期15卷 412-426页

作者： Pedro Romero Alejandro Belanche elisabeth Jiménez Rafael Hueso Eva Ramos-Morales Joan King Salwen Ermias Kebreab David R.Yáñez-Ruiz Estacion Experimental del Zaidin Consejo Superior de Investigaciones Cientificas(CSIC)Profesor Albareda 1Granada 18008Spain Department of Animal Production and Food Sciences University of ZaragozaMiguel Servet 177Saragossa 50013Spain Blue Ocean Barns Inc. Redwood CityCAUSA Department of Animal Science University of CaliforniaDavisCA 95618USA

Background The red macroalgae Asparagopsis is an effective methanogenesis inhibitor due to the presence of halogenated methane(CH_(4))analogues,primarily bromoform(CHBr_(3)).This study aimed to investigate the degradation process of CHBr3 from A taxiformis in the rumen and whether this process is *** in vitro batch culture system was used according to a 2×2 factorial design,assessing two A taxiformis inclusion rates[0(CTL)and 2%DM diet(AT)]and two diets[high-concentrate(HC)and high-forage diet(HF)].Incubations lasted for 72 h and samples of headspace and fermentation liquid were taken at 0,0.5,1,3,6,8,12,16,24,48 and 72 h to assess the pattern of degradation of CHBr_(3) into dibromomethane(CH_(2)Br_(2))and fermentation ***,an in vitro experiment with pure cultures of seven methanogens strains(Methanobrevibacter smithii,Methanobrevibacter ruminantium,Methanosphaera stadtmanae,Methanosarcina barkeri,Methanobrevibacter millerae,Methanorhermobacter wolfei and Methanobacterium mobile)was conducted to test the effects of increasing concentrations of CHBr3(0.4,2,10and 50μmol/L).Results The addition of AT significantly decreased CH_(4) production(P=0.002)and the acetate:propionate ratio(P=0.003)during a 72-h *** concentrations of CHBr_(3) showed a rapid decrease with nearly 90%degraded within the first 3 h of *** the contrary,CH_(2)Br_(2) concentration quickly increased during the first 6 h and then gradually decreased towards the end of the *** CHBr_(3) degradation nor CH_(2)Br_(2) synthesis were affected by the type of diet used as substrate,suggesting that the fermentation rate is not a driving factor involved in CHBr_(3)*** in vitro culture of methanogens showed a dose-response effect of CHBr3 by inhibiting the growth of ***,***,***,***,***,***,and *** The present work demonstrated that CHBr_(3) from *** is quickly degrad

关键词： Bromoform metabolism Dibromomethane metabolism Methane mitigation Methanogens Rumen microbiota Seaweed

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Morphological Identification of the Main Fungi Associated with Sesame in Burkina Faso

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American Journal of Plant Sciences（美国植物学期刊） 2023年第8期

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Integrated adsorption and photocatalytic removal of methylene blue dye from aqueous solution by hierarchical Nb_(2)O_(5)@PAN/PVDF/ANO composite nanofibers

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Nano Materials Science 2024年第1期6卷 96-105页

作者： Aditya Rianjanu Kurniawan Deny Pratama Marpaung elisabeth Kartini Arum Melati Rizky Aflaha Yudha Gusti Wibowo I Putu Mahendra Nursidik Yulianto Januar Widakdo Kuwat Triyana Hutomo Suryo Wasisto Tarmizi Taher Department of Materials Engineering Institut Teknologi SumateraTerusan RyacuduWay HuiJati AgungLampung Selatan35365Indonesia Center for Green and Sustainable Materials Institut Teknologi SumateraTerusan RyacuduWay HuiJati AgungLampung Selatan35365Indonesia Department of Environmental Engineering Institut Teknologi SumateraTerusan RyacuduWay HuiJati AgungLampung Selatan35365Indonesia Department of Physics Faculty of Mathematics and Natural SciencesUniversitas Gadjah MadaSekip Utara PO Box BLS 21Yogyakarta55281Indonesia Department of Mining Engineering Institut Teknologi SumateraTerusan RyacuduWay HuiJati AgungLampung Selatan35365Indonesia Department of Chemistry Institut Teknologi SumateraTerusan RyacuduWay HuiJati AgungLampung Selatan35365Indonesia Research Center for Photonics National Research and Innovation Agency(BRIN)Kawasan Puspitek SerpongTangerang Selatan15314Indonesia Department of Physics Faculty of Mathematics and Natural SciencesUniversitas IndonesiaDepok16424Indonesia PT Nanosense Instrument Indonesia UmbulharjoYogyakarta55167Indonesia Department of Chemical Engineering Faculty of EngineeringUniversitas Gadjah MadaJl Grafika No.2Yogyakarta55281Indonesia

This work presents the development of hierarchical niobium pentoxide(Nb_(2)O_(5))-based composite nanofiber membranes for integrated adsorption and photocatalytic degradation of methylene blue(MB)pollutants from aqueous *** Nb_(2)O_(5) nanorods were vertically grown using a hydrothermal process on a base electrospun nanofibrous membrane made of polyacrylonitrile/polyvinylidene fluoride/ammonium niobate(V)oxalate hydrate(Nb_(2)O_(5)@PAN/PVDF/ANO).They were characterized using field-emission scanning electron microscopy(FE-SEM),X-ray diffraction(XRD)analysis,and Fourier transform infrared(FTIR)*** composite nanofibers possessed a narrow optical bandgap energy of 3.31 eV and demonstrated an MB degradation efficiency of 96%after 480 min contact *** pseudo-first-order kinetic study was also conducted,in which Nb_(2)O_(5)@PAN/PVDF/ANO nanofibers have kinetic constant values of 1.29×10^(-2) min^(-1) and 0.30×10^(-2) min^(-1) for adsorption and photocatalytic degradation of MB aqueous solutions,*** values are 17.7 and 7.8 times greater than those of PAN/PVDF/ANO nanofibers without Nb_(2)O_(5) *** their outstanding photocatalytic performance,the developed membrane materials exhibit advantageous characteristics in recycling,which subsequently widen their practical use in environmental remediation applications.

关键词： Hierarchical nanostructure Composite nanofiber Niobium pentoxide Dye degradation Synergetic adsorption and photocatalysis

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“文化视野”与“经验意识”——迈克尔·苏立文对中西艺术的比较研究

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艺术研究（哈尔滨师范大学艺术学报） 2024年第1期 34-38页

作者：曹子达江苏开放大学设计学院

作为研究中国艺术发展与中国文化传播的西方学者,迈克尔·苏立文是最早一批将中国传统绘画、中国现代艺术的历史传承脉络介绍并普及到西方国家的学术开拓者之一。在主观研究视角上,苏立文更加关注中西方艺术在表现内容、表达形式、象征... 详细信息

作为研究中国艺术发展与中国文化传播的西方学者,迈克尔·苏立文是最早一批将中国传统绘画、中国现代艺术的历史传承脉络介绍并普及到西方国家的学术开拓者之一。在主观研究视角上,苏立文更加关注中西方艺术在表现内容、表达形式、象征寓意及精神、心灵体验上的对比差异,并从中引申出不同文化背景交融碰撞下的借鉴与互通,是基于跨“文化视野”与在彼融合之“经验意识”的学术体系建立,具有超越一般西方文化学者的“感官体验”。

关键词：迈克尔·苏立文 20世纪初期中国美术史中国艺术家中西对比中西融合

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Metastatic pancreatic and lung cancer patient in complete remission following immunotherapy: A case report and review of literature

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 2233-2240页

作者： Joaquina Martínez-Galán Cristina Jiménez-Luna Isabel Rodriguez elisabeth Maza Carlos García-Collado Antonio Rodríguez-Fernández Javier Luis López-Hidalgo Octavio Caba Department of Medical Oncology Virgen de las Nieves University HospitalGranada 18014Spain Instituto de Investigación Biosanitaria Ibs.GranadaGranada 18012Spain Institute of Biopathology and Regenerative Medicine(IBIMER) University of GranadaGranada 18016Spain Pharmacogenetics Unit Virgen de las Nieves University HospitalGranada 18014Spain Department of Medicina Nuclear Virgen de las Nieves University HospitalGranada 18014Spain Department of Anatomía Patológica Virgen de las Nieves University HospitalGranada 18014Spain

BACKGROUND Metastatic pancreatic ductal adenocarcinoma(PDAC)is a lethal malignancy with dispiriting survival *** is a promising approach to many cancer types,but achieves poor outcomes in advanced PDAC due to its immunosuppressive tumor *** describe a case of metastatic PDAC effectively treated with *** SUMMARY We report the case of a 67-year-old woman with unresectable locally advanced PDAC,treated with gemcitabine plus nab-paclitaxel followed by radiotherapy plus *** nine months,pancreatic tumor progression was observed at the level of the hepatic hilum with the appearance of a new pulmonary nodule suggestive of a second primary,confirmed by left lung *** immunotherapy was then initiated with pembrolizumab,an immune checkpoint inhibitor targeting programmed cell death protein-1 that covers the two tumor *** patient showed a complete metabolic response that was maintained throughout the *** patient continues to be disease-free at 5.6 years since the start of *** These results suggest that the administration of pembrolizumab after chemoradiotherapy has a beneficial effect in patients with metastatic *** our knowledge,this is the first reported case of a patient with metastatic PDAC and metastatic lung cancer showing such a long-lasting complete response after pembrolizumab treatment without curative *** studies are required to determine biomarkers that identify PDAC patients most likely to benefit from this immunotherapy.

关键词： Pancreatic ductal adenocarcinoma Lung cancer Immunotherapy Pembrolizumab Programmed cell death protein-1 Case report

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Diagnostic delay in inflammatory bowel diseases in a German population

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World Journal of Gastroenterology 2024年第29期30卷 3465-3478页

作者： elisabeth Blüthner Annalena Dehe Carsten Büning Britta Siegmund Matthias Prager Jochen Maul Alexander Krannich Jan Preiß Bertram Wiedenmann Florian Rieder Raneem Khedraki Frank Tacke Andreas Sturm Anja Schirbel Department of Hepatology and Gastroenterology Campus Virchow-Klinikum and Campus CharitéMitteCharité-Universitätsmedizin BerlinBerlin 10117Germany Department of Gastroenterology Vivantes Klinikum im FriedrichshainBerlin 10249Germany Department of Internal Medicine Krankenhaus WaldfriedeBerlin 14163Germany Department of Gastroenterology Infectious Diseases and RheumatologyCharité-Universitätsmedizin BerlinBerlin 12203Germany Praxis für Gastroenterologie Berlin Zehlendorf Berlin 14195Germany Gastroenterologie am Bayrischen Platz Berlin 10825Germany Clinical Trial Office Charité-Universitätsmedizin BerlinBerlin 10117Germany Department of Gastroenterology Diabetology and HepatologyVivantes Klinikum NeuköllnBerlin 10117Germany Department of Gastroenterology Hepatology and NutritionDigestive Diseases and Surgery InstituteThe Cleveland Clinic FoundationClevelandOH 44195United States Department of Internal Medicine DRK Kliniken Berlin WestendBerlin 14050Germany Gastroenterologie im Havelland Straße der EinheitFalkensee 14612Germany

BACKGROUND Early diagnosis is key to prevent bowel damage in inflammatory bowel disease(IBD).Risk factor analyses linked with delayed diagnosis in European IBD patients are scarce and no data in German IBD patients *** To identify risk factors leading to prolonged diagnostic time in a German IBD *** Between 2012 and 2022,430 IBD patients from four Berlin hospitals were enrolled in a prospective study and asked to complete a 16-item questionnaire to determine features of the path leading to IBD *** diagnostic time was defined as the time from symptom onset to consulting a physician(patient waiting time)and from first consultation to IBD diagnosis(physician diagnostic time).Univariate and multivariate analyses were performed to identify risk factors for each time *** The total diagnostic time was significantly longer in Crohn’s disease(CD)compared to ulcerative colitis(UC)patients(12.0 vs 4.0 mo;P<0.001),mainly due to increased physician diagnostic time(5.5 vs 1.0 mo;P<0.001).In a multivariate analysis,the predominant symptoms diarrhea(P=0.012)and skin lesions(P=0.028)as well as performed gastroscopy(P=0.042)were associated with longer physician diagnostic time in CD *** UC,fever was correlated(P=0.020)with shorter physician diagnostic time,while fatigue(P=0.011)and positive family history(P=0.046)were correlated with longer physician diagnostic *** We demonstrated that CD patients compared to UC are at risk of long diagnostic *** efforts should focus on shortening the diagnostic delay for a better outcome in these patients.

关键词： Diagnostic time Diagnostic delay Crohn’s disease Ulcerative colitis Germany

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