检索结果-南通市图书馆

Myotropic activity and immunolocalization of selected neuropeptides of the burying beetle Nicrophorus vespilloides (Coleoptera: Silphidae)

引用

Insect Science 2019年第4期26卷 656-670页

作者： Arkadiusz Urbanski Jan Lubawy pawel marciniak Grzegorz Rosinski Faculty of Biology Department of Animal Physiology and Development Adam Mickiewicz University in Poznan Poznan Poland

Burying beetles (Nicrophorus sp.) are necrophagous insects with developed parental care. Genome of Nicrophorus vespilloides has been recently sequenced, which makes them interesting model organism in behavioral ecology. However, we know very little about their physiology, including the functioning of their neuroendocrine system. In this study, one of the physiological activities of proctolin, myosuppressin (Nieve? MS), myoinhibitory peptide (Trica-MIP-5) and the short neuropeptide F (Nicve-sNPF) in N. vespilloides have been investigated. The tested neuropeptides were myoactive on N. vespilloides hindgut. After application of the proctolin increased hindgut contraction frequency was observed (EC50 value was 5.47 x 10-8 mol/L). The other tested neuropeptides led to inhibition of N. vespilloides hindgut contractions (Nicve-MS: IC50 = 5.20 x 10~5 mol/L;Trica-MIP-5: IC50 = 5.95 x 10-6 mol/L;Nicvc-sNPF: IC50 = 4.08 x 10-5 mol/L). Moreover, the tested neuropeptides were immunolocalized in the nervous system of N. vespilloides. Neurons containing sNPF and MIP in brain and ventral nerve cord (VNC) were identified. Proctolin-immunolabeled neurons only in VNC were observed. Moreover, MIP-immunolabeled varicosities and fibers in retrocerebral complex were observed. In addition, our results have been supplemented with alignments of amino acid sequences of these neuropeptides in beetle species. This alignment analysis clearly showed amino acid sequence similarities between neuropeptides. Moreover, this allowed to deduce amino acid sequence of N. vespilloides proctolin (RYLPTa), Nicve-MS (QDVDHVFLRFa) and six isoforms ofNicve-MIP (Nicve-MIP-1一 DWNRNLHSWa;Nicve-MIP-2—AWQNLQGGWa;Nicve-MIP-3—AWQNLQGGWa;Nicve-MlP-4—AWKNLNNAGWa;Nicve-MIP-5—SEWGNFRGSWa;Nicve-MIP-6— DPAWTNLKGIWa;and Nicve-sNPF—SGRSPSLRLRFa).

关键词： burying beetles immunolocalization myotropic activity neuropeptides nervous system

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cardioactive properties of Solanaceae plant extracts and pure glycoalkaloids on Zophobas atratus

引用

Insect Science 2015年第2期22卷 251-262页

作者： Emanuela Ventrella pawel marciniak Zbigniew Adamski Grzegorz Rosirnski Szymon Chowanski Patrizia Falabella Laura Scrano SabinoA. Bufo Department of Science University of Basilicata Potenza Italy Department of Animal Physiology and Development Adam Mickiewicz University Poznan Poland Electron and Confocal Microscope Laboratory Adam Mickiewicz University Poznan Poland Department of European and Mediterranean Cultures University of Basilicata Matera Italy

Glycoalkaloids, the biologically active secondary metabolites produced by Solanaceae plants, are natural defenses against animals, insects and fungi. In this paper, the effects of glycoalkaloids present in extracts of Solanaceae plants （potato, tomato and black nightshade） or pure commercial glycoalkaloids on the coleopteran Zophobas atratus E were evaluated by in vitro and in vivo bioassays using heart experimental models. Each tested extract induced a dose-dependent cardioinhibitory effect. The perfusion of Zophobas atratus semi-isolated heart using the highest potato and tomato extract concentration （1 mmol/L） caused irreversible cardiac arrests, while extract from black nightshade pro- duced fast but reversible arrests. Pure commercial glycoalkaloids caused similar but less evident effects compared with extracts. Our results showed that the bioactivity of tested compounds depended on their structure and suggested the existence of synergistic interac- tions when combinations of the main glycoalkaloids of potato and black nightshade were used for trials. Surprisingly, injection of tomato and potato extracts in 1-day-old pupae of Zophobas atratus induced reversible positive chronotropic effects and decreased the duration of the both phases （anterograde and retrograde） of the heart contractile activity. Furthermore, these extracts affected the amplitude of the heart contractions.

关键词： cardioinhibitory effect glycoalkaloid heart perfusion Solanaceae Zophobas atratus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Laser-induced white-light emission from graphene ceramics–opening a band gap in graphene

引用

Light(Science & Applications) 2015年第1期4卷 545-552页

作者： Wieslaw Strek Bartlomiej Cichy Lukasz Radosinski pawel Gluchowski Lukasz marciniak Mikolaj Lukaszewicz Dariusz Hreniak Polish Academy of Sciences Institute of Low Temperatures and Structural Research50-422 WroclawPoland Wroclaw University of Technology Group of Chemical and Biochemical Processes50-370 WroclawPoland

Recent theoretical and experimental studies have indicated the existence of a new stable phase of carbon with mixed sp^(2) and sp^(3) hybridized bonds—diaphite.Such a two-layered structure with sp^(2)/sp^(3) bonds may be observed after the photostimulation of highly oriented pyrolytic graphene with femtosecond laser pulses.This hidden multistability of graphene may be used to create a semiconducting phase immersed in the semimetallic continuum,resulting in bandgap opening.We demonstrate that bandgap opening and light emission from graphene is possible using continuous-wave laser beams with wavelengths from the visible(405 nm)to the near-infrared range(975 nm).We demonstrate that without the application of cooling,the effective temperature of the emitting sample remains lower than 900 K,which is far below the value predicted by the theory of black-body radiation.Moreover,light emission from a graphene sample may be observed at temperatures as low as 10 K.

关键词： ceramics graphene photoluminescence white light

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：