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Integrative Zoology 2016年第1期11卷 25-32页

作者： pavel A.SOROKIn Vyatcheslav V.ROZHnOV Anna U.KRASnEnKO Victor S.LUKAREVSKIY Sergey V.nAIDEnKO Jose A.HERnAnDEZ-BLAnCO A.n.Severtsov Institute of Ecology and Evolution Russian Academy of SciencesMoscowRussia

We used molecular genetic analyses to noninvasively identify individual Amur tigers and define subpopulations of tigers in the Russian Far *** identified 63 individuals after genotyping 256 feces,7 hair and 11 blood samples collected within southern,central and northern Sikhote-Alin,as well as Southwest *** of nuclear DnA at 9 microsatellite loci demonstrated greater genetic similarity between animals from southern and northern Sikhote-Alin(some 500 km apart)than between animals from Ussuriskii State nature Reserve and Southwest Primorye(less than 10 km apart at their nearest point),suggesting that a true barrier exists preventing movements of tigers between Southwest Primorye and the southern Sikhote-Alin Mountains.

关键词： Amur tiger fecal DnA noninvasive genetic sampling Panthera tigris altaica

Multispectral sensing of biological liquids with hollow-core microstructured optical fibres

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Light(Science & Applications) 2020年第1期9卷 346-357页

作者： Timur Ermatov Roman E.noskov Andrey AMachnev Ivan Gnusov Vsevolod Atkin Ekaterina n.Lazareva Sergei V.German Sergey S.Kosolobov Timofei S.Zatsepin Olga V.Sergeeva Julia S.Skibina pavel Ginzburg Valery V.Tuchin Pavlos G.Lagoudakis Dmitry A.Gorin Skolkovo Institute of Science and Technology 3 Nobelya str.Moscow 121205Russia Department of Electrical Engineering Tel Aviv UniversityRamat AvivTel Aviv 69978Israel Light-Matter Interaction Centre Tel Aviv UniversityRamat AvivTel Aviv 69978Israel Saratov State University 83 Astrakhanskaya str.Saratov 410012Russia Tomsk State University 36 Lenin’s av.Tomsk 634050Russia M.V.Lomonosov Moscow State University Leninskie Gory1-3Moscow 119992Russia SPE LLC nanostructured Glass Technology 10150 Let OktjabrjaSaratov 410033Russia Center for Photonics and 2D Materials Moscow Institute of Physics and TechnologyDolgoprudny 141700Russia Institute of Precision Mechanics and Control of the Russian Academy of Sciences 24 Rabochaya str.Saratov 410028Russia

The state of the art in optical biosensing is focused on reaching high sensitivity at a single wavelength by using any type of optical *** common strategy,however,disregards the promising possibility of simultaneous measurements of a bioanalyte’s refractive index over a broadband spectral ***,we address this issue by introducing the approach of in-fibre multispectral optical sensing(IMOS).The operating principle relies on detecting changes in the transmission of a hollow-core microstructured optical fibre when a bioanalyte is streamed through it via liquid *** offers a unique opportunity to measure the refractive index at 42 wavelengths,with a sensitivity up to ~3000 nm per refractive index unit(RIU)and a figure of merit reaching 99 RIU^(−1) in the visible and near-infra-red spectral *** apply this technique to determine the concentration and refractive index dispersion for bovine serum albumin and show that the accuracy meets clinical needs.

关键词： refractive spectral microstructure

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碱激发水泥的类型与特点(英文)

硅酸盐学报 2012年第1期40卷 69-75页

作者：史才军何富强 A.FERnAnDEZ-JIMIEnEZ V.pavel KRIVEnKO Angel PALOMO 湖南大学土木工程学院长沙410082 厦门理工学院土木工程与建筑系厦门361024 Eduardo Torroja Institute Consejo Superior de Investigaciones Cientificas c/Serrano Galvache s/n28033 MadridSpain Kiev national University of Civil Engineering and Architecture 31Vozdukhoflotskii prospectPO Box 161 Kiev 03037Ukraine

基于所含胶凝组份的成分,可以将碱激发水泥分成5种类型:1)碱激发矿渣水泥;2)碱激发波特兰复合水泥;3)碱激发火山灰水泥;4)碱激发石灰-火山灰/矿渣水泥;5)碱激发铝酸钙复合水泥,每种类型碱激发水泥包含几种胶凝体系。综述了这5种碱激发... 详细信息

基于所含胶凝组份的成分,可以将碱激发水泥分成5种类型:1)碱激发矿渣水泥;2)碱激发波特兰复合水泥;3)碱激发火山灰水泥;4)碱激发石灰-火山灰/矿渣水泥;5)碱激发铝酸钙复合水泥,每种类型碱激发水泥包含几种胶凝体系。综述了这5种碱激发水泥的成分和特征。

关键词：碱激发水泥特性微结构耐久性

过氧化氢氧化与阀切换离子色谱法检测食盐中的亚硝酸盐

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药物分析杂志 2023年第12期43卷 2105-2112页

作者：唐嘉怡郑杨杨黄雪婷 nesterenko pavel n 叶明立陈梅兰浙江树人大学生物与环境工程学院杭州310015 Chemistry Department Physical Chemistry DivisionLomonosov Moscow State UniversityMoscow 119991

目的:利用过氧化氢氧化与阀切换离子色谱法测定食盐中亚硝酸盐含量。方法:利用氧化剂(过氧化氢)将食盐样品溶液中的nO_(2)^(-)氧化为nO_(3)^(-),再通过阀切换,洗脱大部分Cl^(-)和Br^(-),收集氧化生成的nO_(3)^(-)于定量环中,定量环中的n... 详细信息

目的:利用过氧化氢氧化与阀切换离子色谱法测定食盐中亚硝酸盐含量。方法:利用氧化剂(过氧化氢)将食盐样品溶液中的nO_(2)^(-)氧化为nO_(3)^(-),再通过阀切换,洗脱大部分Cl^(-)和Br^(-),收集氧化生成的nO_(3)^(-)于定量环中,定量环中的nO_(3)^(-)经氢氧化钾淋洗液进入AS11-HC保护柱(50 mm×4 mm)和分析柱(250 mm×4 mm)进行分离,再进行电导检测,通过检测nO_(3)^(-)的含量间接得到nO_(2)^(-)的含量。结果:在0.01~1.00 mg·L^(-1)的线性范围内,r=0.9999,RSD(n=7)为2.3%,检测限为8.57×10^(-4)mg·L^(-1),回收率为85.2%~111.0%。22个样品中5个样品未检出nO_(2)^(-),其他样品介于6.94~88.70μg·g^(-1)。结论:该实验方法准确、灵敏,可应用于食盐中亚硝酸盐的检测。

关键词：阀切换过氧化氢氧化食盐硝酸盐亚硝酸盐离子色谱

3-Phenyl/Pyridinyl Derivatives of <i>Trans</i>-2-(aryl/heteryl)vinyl-3<i>H</i>-quinazolin-4-ones: Synthesis and Fluorescent Properties

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International Journal of Organic Chemistry 2012年第1期2卷 56-63页

作者： Emiliya V. nosova Tatyana V. Stupina Galina n. Lipunova Marina S. Valova pavel A. Slepukhin Valery n. Charushin Department of Organic Chemistry Chemical Technology Institute Urals Federal University Ekaterinburg Russia Postovsky Institute of Organic Synthesis Ural Branch of the Russian Academy of Sciences Ekaterinburg Russia

novel 3-phenyl/pyridinyl-trans-2-(aryl/heteryl)vinyl-3H-quinazolin-4-ones 3a,b, 4a, 5a, 7a and their 6,7-difluoro de- rivatives 3c,d, 4b, 5b, 7b have been obtained by condensation of the correspondent 2-methylquinazolin-4-ones 1, 6 with aromatic (heterocyclic) aldehydes in the presence of ZnCl2 (AcOna) or by the reaction of 2-methyl-3,1-benzoxazin-4- ones 2 with the Shiff bases. Effects of aryl(heteryl) substituents on photophysical properties of (aryl/heteryl) quinazolinylethenes have been studied.

关键词： Styryl Benzazines Fluorinated Quinazolin-4-Ones UV and FL Spectra X-Ray Trans-cis-Isomerisation 2-Methylquinazolinones 2-Methyl-3 1-benzoxazinones

Celiac disease markers in patients with liver diseases: A single center large scale screening study

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World Journal of Gastroenterology 2012年第43期18卷 6255-6262页

作者： pavel Drastich Eva Honsová Alena Lodererová Marcela Jaresová Aneta Pekáriková Iva Hoffmanová Ludmila Tuková Helena Tlaskalová-Hogenová Julius piák Daniel Sánchez Department of Hepatogastroenterology Institute for Clinical and Experimental Medicine 14021 Prague Czech Republic Department of Clinical and Transplant Pathology Institute for Clinical and Experimental Medicine 14021 Prague Czech Republic Department of Immunology Institute for Clinical and Experimental Medicine 14021 Prague Czech Republic Department of Immunology Institute of Microbiology Academy of Sciences of the Czech Republic 14220 Prague Czech Republic 2nd Department of Internal Medicine 3rd Faculty of Medicine Charles University 10034 Prague Czech Republic

AIM:To study the coincidence of celiac disease, we tested its serological markers in patients with various liver ***:Large-scale screening of serum antibodies against tissue transglutaminase (tTG), and deamidated gliadin using enzyme-linked immunosorbent assay and serum antibodies against endomysium using immunohistochemistry, in patients with various liver diseases (n = 962) and patients who underwent liver transplantation (OLTx, n = 523) was performed. The expression of tTG in liver tissue samples of patients simultaneously suffering from celiac disease and from various liver diseases using immunohistochemistry was carried out. The final diagnosis of celiac disease was confirmed by histological analysis of small-intestinal biopsy. RESULTS:We found that 29 of 962 patients (3%) with liver diseases and 5 of 523 patients (0.8%) who underwent OLTx were seropositive for IgA and IgG anti-tTG antibodies. However, celiac disease was biopsy-diagnosed in 16 patients:4 with autoimmune hepatitis type Ⅰ, 3 with Wilson's disease, 3 with celiac hepatitis, 2 with primary sclerosing cholangitis, 1 with primary biliary cirrhosis, 1 with Budd-Chiari syndrome, 1 with toxic hepatitis, and 1 with non-alcoholic steatohepatitis. Unexpectedly, the highest prevalence of celiac disease was found in patients with Wilson's disease (9.7%), with which it is only rarely associated. On the other hand, no OLTx patients were diagnosed with celiac disease in our study. A pilot study of the expression of tTG in liver tissue using immunohistochemistry documented the overexpression of this molecule in endothelial cells and periportal hepatocytes of patients simultaneously suffering from celiac disease and toxic hepatitis, primary sclerosing cholangitis or autoimmune hepatitis type Ⅰ. COnCLUSIOn:We suggest that screening for celiac disease may be beneficial not only in patients with associated liver diseases, but also in patients with Wilson's disease.

关键词： Tissue transglutaminase Anti-tissue transglutaminase antibodies Autoimmune liver diseases Wilson's disease Celiac disease Liver transplantation

载流子平衡方法提高量子点发光二极管效率的研究

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半导体光电 2020年第5期41卷 667-672页

作者：杨媚郑力为孟琪王鑫梁培袁晓霖唐莹 Sergei I.Pavlov pavel n.Brunkov 刘祖刚中国计量大学光学与电子科技学院杭州310018 中芯集成电路(宁波)有限公司浙江宁波315800 Ioffe物理技术研究所圣彼得堡194021

尝试采用三种方式来平衡载流子的浓度,以提高量子点发光二极管(QLED)的外量子效率等性能:在正装结构(ITO/HIL/HTL/QD/ETL/EIL/金属阴极)的QLED的发光层和电子传输层中间插入超薄聚甲基丙烯酸甲脂(PMMA)电子阻挡层;在空穴注入和传输层方... 详细信息

尝试采用三种方式来平衡载流子的浓度,以提高量子点发光二极管(QLED)的外量子效率等性能:在正装结构(ITO/HIL/HTL/QD/ETL/EIL/金属阴极)的QLED的发光层和电子传输层中间插入超薄聚甲基丙烯酸甲脂(PMMA)电子阻挡层;在空穴注入和传输层方面,通过使用更加优化的HIL等来提高空穴注入和传输几率;在QD发光层方面,用短链配体来置换量子点的长链配体以增加载流子向量子点发光层中的传输效率等。在进行量子点配体交换的同时带来了量子点在正交溶剂中的可溶性优势,有利于QLED器件的全溶液法制备。

关键词：量子点发光二极管电子阻挡层空穴注入层电荷平衡

Monoribbed-Functionalized Macrobicyclic Iron(Ⅱ) Complexes Decorated with Terminal Reactive and Vector Groups: Synthetic Strategy, Chemical Transformations and Structural Characterization

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Chinese Journal of Chemistry 2022年第17期40卷 2089-2105页

作者： Ilya P.Limarev Genrikh E.Zelinskii Svetlana A.Belova pavel V.Dorovatovskii Anna V.Vologzhanina Ekaterina G.Lebed Yan Z.Voloshin nesmeyanov Institute of Organoelement Compounds of the Russian Academy of Sciences 28 Vavilova stMoscow119991 Russia Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences 31 Leninsky prMoscow119991 Russia national Research Center Kurchatov Institute 1 Kurchatova plMoscow123098 Russia

General and straightforward synthetic strategy towards the iron(Ⅱ)clathrochelates with functionalizing substituents in their apical capping and one of the three chelate ribbed fragments was *** hexachloroclathrochelate precursors were prepared by the direct template condensation of dichloroglyoxime with a suitable boronic acid on the Fe^(2+) ion as a matrix and underwent a stepwise nucleophilic substitution with S_(2)-,n_(2)- or O_(2)-bis-nucleophiles,forming the alicyclic or aromatic n_(2)-,S_(2)-or O_(2)-six-membered *** on the reaction conditions and precursor-to-dinucleophile molar ratios,iron(Ⅱ)clathrochelates with one(S,O and n),two(S,O and n)or three(S and O)X_(2)-six-membered ribbed substituent(s)were *** reactivity substantially decreases in a row:Cl_(6)-Cage>Cl_(4)X_(2)-Cage(where X=S,O or,especially,n)>Cl_(2)X_(4)-Cage(where X=S or O).The reactive monoribbed-functionalized clathrochelates underwent further chemical transformations giving the target macrobicyclic complexes with terminal vector *** follows from the single crystal XRD data,their Fen_(6)-coordination polyhedra possess the geometry intermediate between a trigonal prism and a trigonal antiprism(φ=21.3°—25.20°).Fe-n distances vary from 1.887(6)to 1.933(8)Å.Their 3D-molecules,the hydrophobic–hydrophilic balance in which can be tuned using a ribbed functionalization,form in the crystals the intermolecular specific halogen bonds and/or hydrophobic interactions.

关键词： Macrocyclic compounds Clathrochelates Iron complexes Ligand reactivity Targetdelivery

Multiple haploids, triploids, and tetraploids found in modern-day “living fossil” Ginkgo biloba

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Horticulture Research 2018年第1期5卷 190-201页

作者： PetrŠmarda Lucie Horová Ondřej Knápek Heidi Dieck Martin Dieck Katarína Ražná pavel Hrubík Laszlo Orlóci Laszlo Papp Kristýna Veselá pavel Veselý Petr Bureš Department of Botany and Zoology Masaryk UniversityKoltlářská2CZ-61137BrnoCzech Republic Herrenkamper Gärten Herrenkamp 1DE-27254SiedenburgGermany Department of Genetics and Plant Breeding Slovak University of Agriculture in NitraTr.A.Hlinku 294976NitraSlovakia Slovak University of Agriculture in nitra Faculty of Horticulture and Landscape EngineeringDunajská1694911NitraSlovakia Botanical Garden of Eötvös University Illés utca 25BudapestHungary

Ginkgo biloba,the last extant representative of a lineage of Mesozoic gymnosperms,is one of the few seed plants with an exceptionally long(~300 Myr)evolutionary history free of genome-wide duplications(polyploidy).Despite this genome conservatism,we have recently found a viable spontaneous tetraploid Ginkgo sapling during routine screening of several plants,demonstrating that natural polyploidy is possible in *** we provide a much wider flow cytometry survey of ploidy in some European Ginkgo collections,and own seedlings(>2200 individuals and~200 cultivars).We found a surprisingly high level of ploidy variation in modern-day Ginkgo and documented altogether 13 haploid,3 triploid,and 10 tetraploid Ginkgo plants or cultivars,most of them being morphologically distinct from common *** frequently produced polyploid(dihaploid)buds or *** showed some genome size *** surveyed plants provide a unique resource for future Ginkgo research and breeding,and they might be used to accelerate the modern diversification of this nearly extinct plant lineage.

关键词： routine breeding spite

Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure

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Journal of Animal Science and Biotechnology 2019年第2期10卷 463-474页

作者： pavel Horky Sylvie Skalickova Lenka Urbankova Daria Baholet Silvia Kociova Zuzana Bytesnikova Eliska Kabourkova Zuzana Lackova natalia Cernei Milica Gagic Vedran Milosavljevic Vendula Smolikova Eva Vaclavkova pavel n.vrkla pavel Knot Olga Krystofova David Hynek pavel Kopel Jiri Skladanka Vojtech Adam Kristyna Smerkova Department of Animal nutrition and Forage Production Mendel University in BrnoZemedelska 1CZ-613 00 BrnoCzech Republic Department of Chemistry and Biochemistry Mendel University in BrnoZemedelska 1CZ-613 00 BrnoCzech Republic Central European Institute of Technology Brno University of TechnologyPurkynova 123CZ-612 00 BrnoCzech Republic Institute of Animal Science Komenskeho 1239CZ-517 41 Kostelec nad OrliciCzech Republic Department of Animal Breeding Mendel University in BrnoZemedelska 1CZ-613 00 BrnoCzech Republic.

Background: Development of new nanomaterials that inhibit or kil bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles(nPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including ***: Four formulations of zinc nanoparticles(Zn A, Zn B, Zn C, and Zn D) based on phosphates with spherical(Zn A, Zn B)or irregular(Zn C, Zn D) morphology were prepared. The highest in vitro inhibitory effect of our nPs was observed against Staphylococcus aureus(inhibitory concentration values, IC50, ranged from 0.5 to 1.6 mmol/L), fol owed by Escherichia coli(IC500.8–1.5 mmol/L). In contrast, methicil in resistant S. aureus(IC501.2–4.7 mmol/L) was least affected and this was similar to inhibitory patterns of commercial Zn O-based nPs and Zn O. After the successful in vitro testing, the in vivo study with rats based on dietary supplementation with zinc nPs was conducted. Four groups of rats were treated by 2,000 mg Zn/kg diet of Zn A, Zn B, Zn C, and Zn D, for comparison two groups were supplemented by 2,000 mg Zn/kg diet of Zn O-n and Zn O, and one group(control) was fed only by basal diet. The significantly higher(P n level in liver and kidney of al treated groups was found, nevertheless Zn nPs did not greatly influence antioxidant status of rats. However,the total aerobic and coliform bacterial population in rat feces significantly decreased(P n al zinc groups after 30 d of the treatment. Furthermore, when compared to the Zn O group, Zn A and Zn C nanoparticles reduced coliforms significantly more(P nclusions: Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents.

关键词： Aerobic bacteria Antibiotics Coliforms nanomaterials Oxidative stress