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Integrative Zoology 2015年第4期10卷 376-388页

作者： Zheng-Ting ZOU Olga V.UPHYRKINA pavel FOMENKO Shu-Jin LUO College of Life Sciences Peking-Tsinghua Center for Life SciencesPeking UniversityBeijingChina Institute of Biology and Soil Sciences Far Eastern Branch Russian Academy of SciencesVladivostokRussia World Wide Fund for Nature–Russia Amur Branch VladivostokRussia Department of Computational Medicine and Bioinformatics University of MichiganAnn ArborMichiganUSA.

Poaching and trans-boundary trafficking of tigers and body parts are threatening the world’s last remaining wild *** of an efficient molecular genetic assay for tracing the origins of confiscated specimens will assist in law enforcement and wildlife forensics for this iconic flagship *** developed a multiplex genotyping system“tigrisPlex”to simultaneously assess 22 short tandem repeat(STR,or microsatellite)loci and a gender-identifying SRY gene,all amplified in 4 reactions using as little as 1 ng of template *** DNA samples used for between-run calibration,the system generates STR genotypes that are directly compatible with voucher tiger subspecies genetic profiles,hence making it possible to identify subspecies via bi-parentally inherited *** applied“tigrisPlex”to 12 confiscated specimens from Russia and identified 6 individuals(3 females and 3 males),each represented by duplicated samples and all designated as Amur tigers(Panthera tigris altaica)with high *** STR multiplex system can serve as an effective and versatile approach for genetic profiling of both wild and captive tigers as well as confiscated tiger products,fulfilling various conservation needs for identifying the origins of tiger samples.

关键词： Amur tiger genetic multiplex sexing short tandem repeat

Comment on Q-Switched Tm：YAG Laser Intracavity-Pumped by a 1064 nm Laser

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Chinese Physics Letters 2010年第7期27卷 302-302页

作者： pavel Nicolaie National Institute for Laser Plasma and Radiation Physics Laboratory of Solid-State Quantum Electronics Bucharest R-077125 Romania

The paper published in Chin. Phys. Lett. 26 （2009） 124211 reported a Q-switched 2-tim Tm：YAG laser that is intracavity pumped by a Nd： YAG laser with emission at 1.06μm. However, analysis of the experimental setup concludes that only the Nd：YAG laser is Q-switched, and the Tm：YAG laser operates in the free-generation regime. Therefore, Q-switch operation for the 2μm emission wavelength is not realized.

关键词： Lasers Intracavity-Pumped YAG

Effect of a Hybrid Zinc Stearate-Silver System on the Properties of Polylactide and Its Abiotic and the Biotic Degradation and Antimicrobial Activity Thereof

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Chinese Journal of Polymer Science 2018年第8期36卷 925-933页

作者： Gabriela Jandikova Petra Stoplova Antonio Di Martino Petr Stloukal pavel Kucharczyk Michal Machovsky Vladimir Sedlarik Centre of Polymer Systems University Institute Tomas Bata University in Zlin tr. Tomas Bati 5678 760 O1 Zlin Czech Republic

This work investigates the degradation and properties of a thermoplastically prepared composite comprising a polylactide/ hybrid zinc stearate-silver system. The influence of the zinc stearate-silver system on the properties of the composite is investigated by electron microscopy, differential scanning calorimetry and tensile tests. Furthermore, the antimicrobial activities of the systems are examined. The degradation behaviour of the composites is studied in both abiotic and biotic （composting） environments at an elevated temperature of 58 ℃. The results reveal good dispersion of the additive in the PLA matrix, a stabilizing effect exerted by the same on the polylactide matrix during processing, and slight reduction in glass transition temperature. The zinc stearate-silver component also reduces brittleness and extends elongation of the composite. Abiotic hydrolysis is not significantly affected, which is in contrast with pure PLA, although mineralization during the early stage of biodegradation increases noticeably. The composite exhibits antimicrobial activity, even at the lowest dosage of the zinc stearate/silver component （1 wt%）. Moreover, Ag and Zn contents were found to be present in the composite during abiotic hydrolysis, which was demonstrated by minimal diffusion of Ag ions from the matrix and very extensive washing of compounds that contained Zn.

关键词： Polylactide Composite Biodegradation Zinc stearate Silver Antimicrobial

Plasma-enabled sensing of urea and related amides on polyaniline

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Frontiers of Chemical Science and Engineering 2016年第2期10卷 265-272页

作者： Harinarayanan Puliyalil Petr Slobodian Michal Sedlacik Ruhan Benlikaya pavel Riha Kostya (Ken) Ostrikov Uros Cvelbar Jozef Stefan Institute (F4) Jamova cesta 39 1000 Ljubjana Slovenia Jozef Stefan International Postgraduate School Jamova cesta 39 1000 Ljubjana Slovenia Centre of Polymer Systems University Institute Tomas Bata University Trida T. Bati 5678 76001 Zlin Czech Republic Department of Secondary Science and Mathematics Education Faculty of Necatibey Education Balikesir University 10100 Balikesir Turkey Institute of Hydrodynamics Academy of Sciences Pod Patankou 5 166 12 Prague 6 Czech Republic Institute for Future Environments and Institute for Health and Biomedical Innovation School of ChemistryPhysics and Mechanical Engineering Queensland University of Technology Brisbane Queensland 4000 Australia CSIRO-QUT Joint Sustainable Materials and Devices Laboratory Commonwealth Scientific and Industrial Research Organization New South Wales 2070 Australia School of Physics The University of Sydney Sydney New South Wales 2006 Australia

The atmospheric pressure plasma jet （APPJ） was used to enhance the sensitivity of industrially important polyaniline （PANI） for detection of organic vapors from amides. The gas sensing mechanism of PANI is operating on the basis of reversible protonation or deprotonation, whereas the driving force to improve the sensitivity after plasma modifications is unknown. Herein we manage to solve this problem and investigate the sensing mechanism of atmospheric plasma treated PANI for vapor detection of amides using urea as a model. The results from various analytical techniques indicate that the plausible mechanism responsible for the improved sensi- tivity after plasma treatment is operating through a cyclic transition state formed between the functional groups introduced by plasma treatment and urea. This transition state improved the sensitivity of PANI towards 15 ppm of urea by a factor of 2.4 times PANI. This plasma treated compared to the non-treated PANI is promising for the improvement of the sensitivity and selectivity towards other toxic and carcinogenic amide analytes for gas sensing applications such as improving material proces- sing and controlling food quality.

关键词： gas sensing urea PANI amides plasma

Contracted interlayer distance in graphene/sapphire heterostructure

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Nano Research 2015年第5期8卷 1535-1545页

作者： Shiro Entani Liubov Yu. Antipina pavel V. Avramov Manabu Ohtomo Yoshihiro Matsumoto Norie Hirao Iwao Shimoyama Hiroshi Naramoto Yuji Baba pavel B. Sorokin Seiji Sakai Advanced Science Research Center Japan Atomic Energy Agency Tokai Ibaraki 319-1195 Japan Technological Institute for Superhard and Novel Carbon Materials Troitsk Moscow 142190 Russia Moscow Institute of Physics and Technology Dolgoprudny Moscow 141700 Russia Siberian Federal University 79 Svobodniy pr. Krasnoyarsk 660041 Russia Ouantum Beam Science Directorate Japan Atomic Energy Agency Tokai Ibaraki 319-1195 Japan National University of Science and Technology MISiS Moscow 119049 Russia

Direct growth of graphene on insulators is expected to yield significant improvements in performance of graphene-based electronic and spintronic devices. In this study, we successfully reveal the atomic arrangement and electronic properties of a coherent heterostructure of single-layer graphene and α-Al2O3（0001）. The analysis of the atomic arrangement of single-layer graphene on α-Al2O3（0001） revealed an apparentcontradiction. The in-plane analysis shows that single-layer graphene grows not in a single-crystalline epitaxial manner, but rather in polycrystalline form, with two strongly pronounced preferred orientations. This suggests relatively weak interfacial interactions are operative. However, we demonstrate that unusually strong physical interactions between graphene and α-Al2O3（0001） exist, as evidenced by the small separation between the graphene and the α-Al2O3（0001） surface. The interfacial interaction is shown to be dominated by the electrostatic forces involved in the graphene n-system and the unsaturated electrons of the topmost O layer of α-Al2O3（0001）, rather than the van der Waals interactions. Such features causes graphene hole doping and enable the graphene to slide on the α-Al2O3（0001） surface with only a small energy barrier despite the strong interfacial interactions.

关键词： graphene sapphire chemical vapor deposition graphene/insulator interface

Photocatalytic and electrochemical properties of single-and multi-layer sub-stoichiometric titanium oxide coatings prepared by atmospheric plasma spraying

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Journal of Advanced Ceramics 2016年第2期5卷 126-136页

作者： pavel CTIBOR Ramachandran Chidambaram SESHADRI Jiri HENYCH Vaclav NEHASIL Zdenek PALA Jiri KOTLAN Materials Engineering Department Institute of Plasma Physics AS CR Za Slovankou 3 Prague 8 Czech Republic Center for Thermal Spray Research Department of Materials Science & Engineering The State University of New York at Stony Brook NY 11794-2275 USA Institute of Inorganic Chemistry AS CR Husinec-Rez 250 68 Czech Republic Department of Surface and Plasma Science Faculty of Mathematics and Physics Charles University V Holesovickach 2 Prague 8 Czech Republic

We studied the photocatalytic and electrochemical impedance properties of two different sub-stoichiometric titania powders deposited using plasma spraying. Two different commercial powders with markedly diverse mean size as well as size distribution were chosen. Thermal oxidation of these as-received powders was carried out to restore the O/Ti ratio to be 2.0. By this way, another two kinds of feedstock powders were obtained. Total of four kinds of feedstock powders were used in the experiments. All powders were sprayed using identical plasma spraying parameters. For some spraying runs, carbon steel served as the substrate, and for others, the previously sprayed titania coating from a different kind of feedstock. Combinations of single- and double-layer coatings were studied. Porosity, microstructure, phase composition, chemical composition, band gap based on reflectance measurement, and photocatalytic activity were examined. Electrochemical impedance spectroscopy demonstrated substantial differences between samples sprayed from the fine and coarse powders. Coatings from oxidized powders were slightly more photocatalytic. High spraying distance used for several of them was good for obtaining low band gap, whereas surface roughness and phase composition were not substantially different compared to standard spraying distance coatings.

关键词： titanium dioxide stoichiometry feedstock photocatalysis spectroscopic techniques

Genetic determination of irritable bowel syndrome

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World Journal of Gastroenterology 2008年第43期14卷 6636-6640页

作者： Cristina Hotoleanu Radu Popp Adrian pavel Trifa Laurentiu Nedelcu Dan L Dumitrascu 2nd Medical Department University of Medicine and Pharmacy Iuliu HatieganuCluj-Napoca 400 006Romania University of Medicine and Pharmacy Iuliu Hatieganu Genetic DepartmentCluj-Napoca 400 006Romania Department of Internal medicine University Transilvania BrasovCluj-Napoca 400 006Romania

Irritable bowel syndrome （IBS） is a common functional gastrointestinal disorder. According to the Rome m criteria, IBS is defined as recurrent abdominal pain or discomfort for at least 3 d per month during the previous 3 mo associated with two or more of the following symptoms： improvement with defecation, onset associated with a change in the frequency of stool and/or onset associated with a change in form or appearance of stool. There is growing evidence regarding the genetic contribution in IBS, however the precise etiology of IBS is still unknown. The evaluation of the genetic influence is based on twin studies, familial aggregation and genetic epidemiological investigations. Most studies showed a concordance for IBS significantly greater in monozygotic than in dizygotic twins. The majority of the studies have shown that familial aggregation may represent exposures to a similar environment, as well as the influence of genetic factors. Whereas no specific gene has been identified in association with IBS, recent studies have noticed the importance of polymorphisms in the promoter region of the serotonin reuptake transporter gene, G-protein beta 3 subunit gene （C825T）, cholecystokinin receptor （CCKAR gene 779T〉C）, and high-producer tumor necrosis factor genotype. Further studies are necessary to determine how genetic factors influence the clinical manifestations and therapeutical response in IBS patients.

关键词： Irritable bowel syndrome Genetic factors Gene polymorphisms Twin studies Familial aggregation

Mathematical Modeling of Heat Transfer Processes of Coal Waste Combustion in a Chamber of Automated Energy Generating Complex

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Journal of Thermal Science 2013年第2期22卷 174-179页

作者： Sergey P. Mochalov Sergey N. Kalashnikov pavel S. Mochalov Guolin Song Guoyi Tang Siberian State Industrial University Advanced Materials Institute Graduate School at ShenzhenTsinghua University

The automated energy generating complex allows obtaining heat energy from waste coal-water slurry fuel （WCF） that is a mixture of fine coal particles from coal enrichment wastes with water. The mixture is blown into the swirl chamber under the pressure through the special sprayers. The received heat energy is used in different ways. One of the important issues is to estimate the heat losses through the walls of this chamber. In this paper we solved the boundary problem of mathematical physics to estimate the temperature fields in the walls of the swirl chamber. The obtained solution allows us to estimate the heat losses through the waUs of the swid chamber. The task of the mathematical physics has been solved by a numerical finite-difference method. The method for solving this prob- lem can be used in the calculation of temperature fields and evaluation of heat losses in other thermal power units.

关键词： heat transfer heat conduction equation boundary conditions the heat transfer coefficient tempera-ture distribution.

Comparing the removal of polycyclic aromatic hydrocarbons in soil after different bioremediation approaches in relation to the extracellular enzyme activities

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Journal of Environmental Sciences 2019年第2期31卷 249-258页

作者： Zdeněk Ko?ná? Tereza ?ástková Lucie Wiesnerová Luká? Praus Ivan Jablonsky Martin Koudela pavel Tlusto? Department of Agroenvironmental Chemistry and Plant Nutrition Faculty of AgrobiologyFood and Natural ResourcesCzech University of Life Sciences Prague Department of Horticulture Faculty of AgrobiologyFood and Natural ResourcesCzech University of Life Sciences Prague

A 120-day experiment was conducted to compare the removal of polycyclic aromatic hydrocarbons(PAHs) from agricultural soil after natural attenuation(NA), phytoremediation(P), mycoremediation(M), and plant-assisted mycoremediation(PAM) approaches in relation to the extracellular enzyme activities in soil. The NA treatment removed the total soil PAH content negligibly. The P treatment using maize(Zea mays) enhanced only the removal of low and medium molecular PAHs. The Pleurotus ostreatus cultivated on 30–50 mm wood chip substrate used in M treatment was the most successful in the removal of majority PAHs. Therefore,significantly(p < 0.05) highest total PAH removal by 541.4 μg/kg dw(dry weight)(36%) from all tested M treatments was observed. When using the same fungal substrate together with maize in PAM treatment, the total PAH removal was not statistically different from the previous M treatment. However, the maize-assisted mycoremediation treatment significantly boosted fungal biomass, microbial and manganese peroxidase activity in soil which strongly correlated with the removal of total PAHs. The higher PAH removal in that PAM treatment could be reflected in the following post-harvest time. Our suggested M and PAM approaches could be promising in situ bioremediation strategies for PAH-contaminated soils.

关键词： PAHs Biodegradation White-rot fungi Ligninolytic enzymes Microbial activity

3D Raman mapping as an analytical tool for investigating the coatings of coated drug particles

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Journal of Pharmaceutical Analysis 2023年第3期13卷 276-286页

作者： Georgia Koutentaki pavel Krýsa Dan Trunov Tomás Pekarek Marketa Pislova Miroslav Soos Department of Chemical Engineering University of Chemistry and Technology PraguePrague 616628Czech Republic Zentiva k.s.Prague 1010237Czech Republic

The properties of dry-coated paracetamol particles(fast-dissolving model drug)with carnauba wax particles as the coating agent(dissolution retardant)were *** mapping technique was used to non-destructively examine the thickness and homogeneity of coated *** results showed that the wax existed in two forms on the surface of the paracetamol particles,forming a porous coating layer:i)whole wax particles on the surface of paracetamol and glued together with other wax surface particles,and ii)deformed wax particles spread on the *** of the final particle size fraction(between 100 and 800 mm),the coating thickness had high variability,with average thickness of 5.9±4.2 *** ability of carnauba wax to decrease the dissolution rate of paracetamol was confirmed by dissolution of powder and tablet *** dissolution was slower for larger coated *** further reduced the dissolution rate,clearly indicating the impact of subsequent formulation processes on the final quality of the product.

关键词： Raman mapping Dry-coating Dissolution Particle coating thickness Polymers