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Benchmarking two Saccharomyces cerevisiae laboratory strains for growth and transcriptional response to methanol

Synthetic and Systems Biotechnology 2019年第4期4卷 180-188页

作者： Monica I.Espinosa thomas C.Williams Isak S.Pretorius Ian t.paulsen ARC Centre of Excellence in Synthetic Biology Department of Molecular SciencesMacquarie UniversityNSWAustralia CSIRO Synthetic Biology Future Science Platform CanberraACT2601Australia

One-carbon compounds,such as methanol,are becoming potential alternatives to sugars as feedstocks for the biological production of chemicals,fuels,foods,and *** biological production often requires extensive genetic manipulation of a microbial host strain,making well-characterised and geneticallytractable model organisms like the yeast Saccharomyces cerevisiae attractive targets for the engineering of methylotrophic *** strains S288C and *** are the two best-characterised and most widely used hosts for yeast synthetic biology and metabolic engineering,yet they have unpredictable metabolic phenotypes related to their many genomic *** therefore sought to benchmark these two strains as potential hosts for engineered methylotrophic metabolism by comparing their growth and transcriptomic responses to *** had improved growth in the presence of methanol relative to the S288C derivative *** *** transcriptome also had a specific and relevant response to methanol that was either absent or less pronounced in the BY4741 *** response included up-regulation of genes associated with mitochondrial and peroxisomal metabolism,alcohol and formate dehydrogenation,glutathione metabolism,and the global transcriptional regulator of metabolism ***-expression of MIG3 enabled improved growth in the presence of methanol,suggesting that MIG3 is a mediator of the superior *** strain *** was therefore identified as a superior strain for the future development of synthetic methylotrophy in ***.

关键词： Yeast Methylotrophy Synthetic biology Metabolic engineering MIG3 Methanol

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基于势流理论和OpenFOAM的耦合模型对多向不规则波浪的模拟

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水科学进展 2016年第1期27卷 88-99页

作者：季新然柳淑学 Bo t.paulsen Harry B.BINGHAM 大连理工大学海岸和近海工程国家重点实验室辽宁大连116024 丹麦技术大学机械学院代尔夫特三角洲研究中心

基于势流理论的数值水池可以快速计算波浪的传播及其对建筑物的作用,但是势流理论是基于波浪的无黏性假设的,而在工程中,通常需要在固体边界及波浪破碎的区域考虑黏性效应。针对基于求解Navier-Stokes方程的黏性水池计算量较大、速度较... 详细信息

基于势流理论的数值水池可以快速计算波浪的传播及其对建筑物的作用,但是势流理论是基于波浪的无黏性假设的,而在工程中,通常需要在固体边界及波浪破碎的区域考虑黏性效应。针对基于求解Navier-Stokes方程的黏性水池计算量较大、速度较慢的缺点,采用耦合的方法模拟多向不规则波浪的传播,即在外域通过基于势流理论的数值水池产生多向不规则波浪,内域采用求解Navier-Stokes方程和流体体积法(VOF方法)对自由表面进行追踪,通过外域所提供的边界波浪,内域计算可以在较小区域进行计算,从而达到减少计算量、提高计算效率的目的。

关键词：势流理论 OpenFOAM 耦合多向不规则波浪

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标准蛋白和高蛋白小麦的农艺品质与其营养体中氮磷含量的关系

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新疆农业科学 1986年第6期 46-47页

作者： D.L.Fjell,G.M.paulsen,t.L.Walter,J.R.Lawless,赵广才美美美美

人们对小麦籽粒蛋白质的含量及其营养价值愈来愈重视,很多人还试图增加小麦籽粒中的蛋白质含量.Seth等人报道,高蛋白和标准蛋白小麦营养体的全氮含量在抽穗前无甚差别,抽穗后高蛋白品种穗中氮的增加比标准蛋白品种更多.Meneal等人在5个... 详细信息

人们对小麦籽粒蛋白质的含量及其营养价值愈来愈重视,很多人还试图增加小麦籽粒中的蛋白质含量.Seth等人报道,高蛋白和标准蛋白小麦营养体的全氮含量在抽穗前无甚差别,抽穗后高蛋白品种穗中氮的增加比标准蛋白品种更多.Meneal等人在5个春小麦品种中发现,它们营养体中的含氮量没有不同,但籽粒中蛋白质含量却有差别,只是变化范围很小.Johnson等人报道,Warrior小麦植株含氮量最高,但其籽粒含氮量最低;Warrior比其它品种能吸收更多的土壤有效氮,但却不能将吸收的土壤有效氮全部转移到籽粒中去.高蛋白品种与标准

关键词：营养体含磷量植株体标准蛋白含P量高蛋白小麦籽粒产量开花期拔节期

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卵巢上皮肿瘤患者的症状及收诊科室

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世界核心医学期刊文摘（妇产科学分册） 2005年第5期1卷 60-60页

作者： paulsen t K.rn J Kj.rheim K 朱晓明

Objective: to study referral to hospital units and the clinical characteristic s of women with epithelial ovarian tumors in a prospective, population-based st udy. Methods: Clinical information on all women diagnosed with epithelial invasi ve (n=486) and borderline ovarian tumors (n=137) in Norway during 2002 was deriv ed from notifications to the Cancer Registry of Norway and medical, surgical and histopathological records. Results: Sixty-one percent of women with invasive o varian tumors were initially referred to gynecology units. the 38%of women refe rred to surgical and medical units were more likely to have symptoms as ‘bowel irregularity’, ‘pain outside the abdominal cavity’, ‘persisting fatigue’and ‘respiratory difficulties’. these women were older, had lower performance sta tus and had a delay in treatment of 20 and 24 days respectively compared to 11 a t gynecology units. Conclusion: Greater awareness of the symptoms of ovarian can cer might lead to earlier diagnosis and treatment and thus possibly improve surv ival.

关键词：卵巢上皮卵巢肿瘤患者肠紊乱侵袭性呼吸困难大样本研究

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MR-GUIDED PULSE OXIMEtRY IMAGING OF BREASt IN VIVO

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Journal of Innovative Optical Health Sciences 2011年第2期4卷 199-208页

作者： ZHIQIU LI SHUDONG JIANG VENKAtARAMANAN KRISHNASWAMY SCOtt CDAVIS SUBHADRA SRINIVASAN KEItH D.paulsen BRIAN W.POGUE thayer School of Engineering Dartmouth College Hanover NH 03755USA

A near-infrared(NIR)tomography system with spectrally-encoded sources in two wavelength bands was built to quantify the temporal oxyhemoglobin and deoxyhemoglobin contrast in breast tissue at a 20 Hz *** system was integrated into a 3t magnetic resonance(MR)imaging system through a customized breast coil interface for simultaneous optical and MRI *** this configuration,the MR images provide breast tissue structural information for NIR spectroscopy of adipose andfibro-glandular tissue in *** characterization performance of the NIR system was verified through dynamic phantom *** human subjects were imaged withfinger pulse oximeter(PO)plethysmogram synchronized to the NIR system to provide a frequency-locked *** the raw data from the NIR system and the recovered absorption coefficients of the breast at two wavelengths showed the same frequency of about 1.3 Hz as the PO *** frequency lock-in approach provided a practical platform for MR-localized recovery of small pulsatile variations of oxyhemoglobin and deoxyhemoglobin in the breast,which are related to the heartbeat and vascular resistance of the tissue.

关键词： High frame-rate fast imaging MR-guided near-infrared tomography breast hemodynamics

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叶绿素（a/b）捕光蛋白复合体（LHCIIb）可以从完全变性的脱辅基蛋白状态进行体外重组

叶绿素（a/b）捕光蛋白复合体（LHCIIb）可以从完全变性的脱辅基...

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中国植物生理学会全国学术年会暨成立40周年庆祝大会

作者：杨春虹 Ruth Horn Harald paulsen Institut f. Allgemeine Botanik der Johannes-Gutenberg-Universit?t M llerweg 6 D-55099 Mainz Germany Institut f. Allgemeine Botanik der Johannes-Gutenberg-Universit?t M llerweg 6 D-55099 Mainz Germany Institut f. Allgemeine Botanik der Johannes-Gutenberg-Universit?t M llerweg 6 D-55099 Mainz Germany

＜正＞与水溶性蛋白相比,关于膜蛋白的体外重组的特性所知较少。原因之一是迄今为止,可以成功的进行体外重组的膜蛋白相对较少。很多水溶性蛋白可以在尿素或盐酸肌（Gnd）溶液里完全变性,然后稀释变性条件,再行复性。一些主要含β-折...

＜正＞与水溶性蛋白相比,关于膜蛋白的体外重组的特性所知较少。原因之一是迄今为止,可以成功的进行体外重组的膜蛋白相对较少。很多水溶性蛋白可以在尿素或盐酸肌（Gnd）溶液里完全变性,然后稀释变性条件,再行复性。一些主要含β-折叠结构的膜蛋白,如细菌外膜上的OmpA,或OmpF也可以利用尿素或Gnd变性,然后借助表面活性剂形成的微束,或脂质体进行复性。有些主要含α-螺旋结构的膜蛋白:如半乳糖转运酶也可以利用相同的方法进行重组,但重组的产量很低。大多数主要含α-螺旋结构的膜蛋白不能从Gnd溶液中变性的蛋白进行重组。这些蛋白的重组主要通过将蛋白溶解于一种表面活性剂（SDS）溶液中,然后再进行重组。SDS可以稳定膜蛋白中一部分α-螺旋结构,比如LHCⅡb在SDS溶液中已经具有50%的所有的α-螺旋结构。因此,很容易推断,α-螺旋结构的存在,是某些膜蛋白进行体外重组的先决条件。

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Interdisciplinary management of central nervous system metastasis and neoplastic meningitis:recent developments and future perspectives

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Journal of Cancer Metastasis and treatment 2016年第1期2卷 163-175页

作者： Ghazaleh tabatabai Marilin Koch Cristiana Roggia Juliane Ebert Claus Garbe Friedegund Meier Sara Brucker Eva Maria Grischke Diethelm Wallwiener Robert Möhle Lothar Kanz Walter Erich Aulitzky Ulrike Ernemann Christian laFougere Konstantin Nikolaou Bernd Pichler Jens Schittenhelm Manuela Neumann Falko Fend Stefan Czemmel Sven Nahnsen Frank paulsen Daniel Zips Maike van Lessen Hans-Otto Karnath Ulf Ziemann Constantin Roder Marco Skardelly Jürgen Bernd Honegger Marcos tatagiba Interdisciplinary Division of Neuro-Oncology Departments of Vascular Neurology and NeurosurgeryUniversity Hospital TübingenHertie Institute for Clinical Brain ResearchEberhard Karls University72076 TübingenGermany Division of Dermato-Oncology Department of DermatologyUniversity Hospital TübingenEberhard Karls University72076 TübingenGermany Department of Gynecology and Ostetrics University Hospital TübingenEberhard Karls University72076 TübingenGermany Department of Internal Medicine(II) University Hospital TübingenEberhard Karls University72076 TübingenGermany Department of Hematology and Oncology Robert Bosch Hospital StuttgartStuttgartGermanyComprehensive Cancer CenterTübingen StuttgartGermany Department of Diagnostic and Interventional Neuroradiology University Hospital TübingenEberhard Karls University72076 TübingenGermany Department of Nuclear Medicine University Hospital TübingenEberhard Karls University72076 TübingenGermany Department of Diagnostic and Interventional Radiology University Hospital TübingenEberhard Karls University72076 TübingenGermany Department of Preclinical Imaging and Radiopharmacy University Hospital TübingenEberhard Karls University72076 TübingenGermany Department of Pathology and Neuropathology University Hospital TübingenEberhard Karls University72076 TübingenGermany Department of Radiation Oncology University Hospital TübingenEberhard Karls University72076 TübingenGermany Division of Neuropsychology University Hospital TübingenHertie Institute for Clinical Brain ResearchEberhard Karls University72076 TübingenGermany Center for CNS tumors Comprehensive Cancer Center Tübingen StuttgartGermany Center for Personalized Medicine University Hospital TübingenEberhard Karls UniversityTübingenGermany Center for Dermato-Oncology University Hospital DresdenGermany Quantitative Biology Center(QBiC) Eberhard Karls UniversityTübingenGermany DKtK DKFZ partner site Tübingen.18Department of ImmunologyEberhard Karls University TübingenGermany

the incidence of metastatic disease in the central nervous system(CNS)is *** to current estimates,up to a third of adult cancer patients will suffer from CNS *** evidence-based data from prospective randomized trials are rare,however,because CNS metastasis patients were often excluded from clinical trial *** management of CNS metastasis patients is therefore rather ill-defined and an interdisciplinary *** basic and translational science data have begun contributing to a more profound understanding of the molecular mechanisms leading to invasion of tumor cells into the *** report reviews advances,challenges,and perspectives in this field.

关键词： Brain tumor central nervous system metastases interdisciplinary management

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