检索结果-南通市图书馆

A novel sampling method for the investigation of gut microbiota

World Journal of Gastroenterology 2007年第29期13卷 3990-3995页

作者： Alina Guimares Quintanilha Bruno Zilberstein Manoel A A Santos Denis Pajecki Eduardo Guimares Hourneaux Moura paulo roberto Arruda alves Fauze Maluf-Filho Ivan Cecconello Digestive Surgery Division Department of Gastroenterology and Institute of Biomedical Sciences Department of Microbiology So Paulo University Medical School So Paulo SP Brazil

AIM： In order to characterize the qualitative and quantitative microorganisms in different sites of the lower digestive tract （LDT） in healthy volunteers, a specific technique was developed for collecting mucous of the distal ileum, colon and rectum. METHODS： A polyethylene tube was designed to go through the colonoscope channel with a No. 8 French tube. In order to avoid internal contamination, the distal extremity was protected with a membrane of microfilm after being sterilized in ethilene oxid. To facilitate the aspiration of a precise volume, its interior was coated with silicone. One hundred rnicrolliter （0.1 mL） sample of mucous was collected and transferred into an Eppenddorff tube containing nine hundred rnicrolliter （0.9mL） of VMGA-3 （viable medium of Goteborg）. This procedure was repeated at each site of the LDT with a new sterilized catheter. RESULTS： All sites revealed the ＂non pathogenic＂ anaerobic bacteria Veillonella sp （average 10s colony forming units/mL-CFU/mL）, allowing to conclude an environment of low oxidation-reduction potential （redox） in the LDT. It was also characterized the presence of Klebisiella sp with significant statistical predominance （SSP） in the ileum. Enterobacter sp was found with SSP in the sigrnoid colon, Bacteroides sp non-pigmented （npg） and Ecoli with SSP in the sigrnoid colon and rectum, Enterococcus sp and Lactobacillus sp with SSP in the rectum, all in a mean concentration of 10s CFU/mL CONCLUSION： This procedure is feasible and efficient and can point out a similar distribution of the aerobic and anaerobic bacteria with the presence of biological markers of normal microbiota in the LDT.

关键词： Colony count Microbial Fungi Aerobic bacteria Anaerobic bacteria Gastrointestinal endoscopy Large Intestine Bacterial translocation Microbiology Catheterization

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Applying a Context-based Method to Build a Knowledge Graph for the Blue Amazon

引用

Data Intelligence 2024年第1期6卷 64-103页

作者： Pedro de Moraes Ligabue Anarosa alves Franco Brandão Sarajane Marques Peres Fabio Gagliardi Cozman paulo Pirozelli Escola Politécnica Universidade de São PauloRua Visconde da Luz60São PauloSão Paulo 05508-010Brazi Escola Politécnica Universidade de São PauloAv.Prof.Luciano Gualbertotv 3158São Paulo 05508-010Brazil Escola de Artes Ciências e Humanidades Universidade de São PauloRua Arlindo Béttio1000São Paulo 03828-000Brazil Instituto de Estudos Avançados Universidade de São PauloRua da Praça do Relógio109São Paulo 05508-050Brazil

Knowledge graphs are employed in several tasks,such as question answering and recommendation systems,due to their ability to represent relationships between *** constructing such a graphs,however,remains an unresolved challenge within knowledge *** tackle this challenge,we propose CtxKG,a method specifically aimed at extracting knowledge graphs in a context of limited resources in which the only input is a set of unstructured text *** is based on OpenIE(a relationship triple extraction method)and BERT(a language model)and contains four stages:the extraction of relationship triples directly from text;the identification of synonyms across triples;the merging of similar entities;and the building of bridges between knowledge graphs of different *** method distinguishes itself from those in the current literature(i)through its use of the parse tree to avoid the overlapping entities produced by base implementations of OpenIE;and(ii)through its bridges,which create a connected network of graphs,overcoming a limitation similar methods have of one isolated graph per *** compare our method to two others by generating graphs for movie articles from Wikipedia and contrasting them with benchmark graphs built from the OMDb movie *** results suggest that our method is able to improve multiple aspects of knowledge graph *** also highlight the critical role that triple identification and named-entity recognition have in improving the quality of automatically generated graphs,suggesting future paths for ***,we apply CtxKG to build BlabKG,a knowledge graph for the Blue Amazon,and discuss possible improvements.

关键词： knowledge graph word embeddings relationship triple extraction Blue Amazon Atlantic Ocean Brazilian coast

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Remote Sensing Applied to the Evaluation of Spatial and Temporal Variation of Water Quality in a Coastal Environment, Southeast Brazil

引用

Journal of Geographic Information System 2019年第5期11卷 500-521页

作者： Pedro Bettencourt Julio Cesar Wasserman Fábio Ferreira Dias paulo roberto alves Dandara Bernardino Bezerra Camila Américo Santos Luis Perez Zotes Sérgio Ricardo Barros Post-Graduate Program in Sustainable Systems Management UFF Niterói Brazil Post-Graduate Program in Geochemistry UFF Niterói Brazil Department of Geoenvironmental Analysis UFF Niterói Brazil Pós graduao em Biologia Marinha e Ambientes Costeiros UFF Niterói Brazil Pós graduao em Engenharia de Biossistemas UFF Niterói Brazil.

The monitoring of water quality in large coastal regions demands great analytical efforts through the collection of many samples, over long periods. Remote sensing is a reliable tool that can provide valuable information on the spatial and temporal variations of environmental parameters, particularly turbidity and chlorophyll a. The aim of the present research was to evaluate the spatial and temporal distribution of water quality from 2005 to 2017 along the north coast of São paulo and its responses to the implementation of industrial developments and to variations in rainfall. Fifty-two MODIS images were used, showing concentrations of chlorophyll a and turbidity, in the dry season and wet season, from 2005 to 2017. The results showed that dilution processes (due to rainfall) control chlorophyll a concentrations. However, a notable increase in concentrations could be identified after the installation of some of the developments in the region, particularly roads and ports. Turbidity was also shown to be affected by dilution processes, and during the wet season this parameter presented lower values. No effect in the results of turbidity could be identified from the installation of roads or ports, showing that vegetation cover exerts an important control on the erosional processes.

关键词： Water Quality Chlorophyll a Turbidity MODIS Remote Sensing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nicotinic cholinergic receptors in esophagus:Early alteration during carcinogenesis and prognostic value

引用

World Journal of Gastroenterology 2016年第31期22卷 7146-7156页

作者： Marina Chianello Nicolau Luis Felipe Ribeiro Pinto Pedro Nicolau-Neto paulo roberto alves de Pinho Ana Rossini Tatiana de Almeida Simao Sheila Coelho Soares Lima Departamento de Bioquímica Instituto de Biologia Roberto Alcantara GomesUniversidade do Estado do Rio de Janeiro Programa de Carcinogênese Molecular Instituto Nacional de CancerCoordenacao de Pesquisa Gastroenterologia Hospital Universitário Pedro ErnestoUniversidade do Estado do Rio de Janeiro

AIM: To compare expression of nicotinic cholinergic receptors(CHRNs) in healthy and squamous cell carcinoma-affected esophagus and determine the prognostic ***: We performed RT-q PCR to measure the expression of CHRNs in 44 esophageal samples from healthy individuals and in matched normal surrounding mucosa, and in tumors from 28 patientsdiagnosed with esophageal squamous cell carcinoma(ESCC). Next, we performed correlation analysis for the detected expression of these receptors with the habits and clinico-pathological characteristics of all study participants. In order to investigate the possible correlations between the expression of the different CHRN subunits in both healthy esophagus and tissues from ESCC patients, correlation matrices were generated. Subsequently, we evaluated whether the detected alterations in expression of the various CHRNs could precede histopathological modifications during the esophageal carcinogenic processes by using receiver operating characteristic curve analysis. Finally, we evaluated the impact of CHRNA5 and CHRNA7 expression on overall survival by using multivariate ***: CHRNA3, CHRNA5, CHRNA7 and CHRNB4, but not CHRNA1, CHRNA4, CHRNA9 or CHRNA10, were found to be expressed in normal(healthy) esophageal mucosa. In ESCC, CHRNA5 and CHRNA7 were overexpressed as compared with patient-matched surrounding non-tumor mucosa(ESCC-adjacent mucosa; P < 0.0001 and P = 0.0091, respectively). Positive correlations were observed between CHRNA3 and CHRNB4 expression in all samples analyzed. Additionally, CHRNB4 was found to be differentially expressed in the healthy esophagus and the normalappearing ESCC-adjacent mucosa, allowing for distinguishment between these tissues with a sensitivity of 75.86% and a specificity of 78.95%(P = 0.0002). Finally, CHRNA5 expression was identified as an independent prognostic factor in ESCC; patients with high CHRNA5 expression showed an increased overall survival, in comparison with those

关键词： Nicotinic cholinergic receptors Esophagus Esophageal squamous cell carcinoma Tobacco Alcohol Gene expression

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of resistance training volume on body adiposity,metabolic risk,and inflammation in postmenopausal and older females:Systematic review and meta-analysis of randomized controlled trials

引用

Journal of Sport and Health Science 2024年第2期13卷 145-159页

作者： paulo Ricardo Prado Nunes Pamela Castro-e-Souza Anselmo alves de Oliveira Bruno de Freitas Camilo Gislaine Cristina-Souza Lucio Marques Vieira-Souza Marcelo Augusto da Silva Carneiro Department of Body and Human Movement Minas Gerais State University(UEMG)37902-092 PassosMGBrazil University Center of Planalto de Araxa(UNIARAXA) 38180-129 AraxaMGBrazil Physical Activity Health and Ageing Research Group(GPASE)Minas Gerais State University(UEMG)37902-092 PassosMGBrazil Exercise and Nutrition Research Group(GPEN) Minas Gerais State University(UEMG)37902-092 PassosMGBrazil Sport Sciences and Physical Exercise Study and Research Group(GEPCEEX) Minas Gerais State University(UEMG)37902-092 PassosMGBrazil Metabolism Nutrition and Exercise LaboratoryPhysical Education and Sport CenterLondrina State University(UEL)86050-070 LondrinaPRBrazil Postgraduate Program in Physical Education Federal University of Sergipe(UFS)49107-230 Sao CristovaoSEBrazil

Purpose:This meta-analytical study aimed to explore the effects of resistance training(RT) volume on body adiposity,metabolic risk,and inflammation in postmenopausal and older ***:A systematic search was performed for randomized controlled trials in PubMed,Scopus,Web of Science,and *** controlled trials with postmenopausal and older females that compared RT effects on body adiposity,metabolic risk,and inflammation with a control group(CG) were *** reviewers selected the studies,extracted the data,and performed the risk of bias and certainty of the evidence(Grading of Recommendations,Assessment,Development,and Evaluation(GRADE)) *** body and abdominal adiposity,blood lipids,glucose,and C-reactive protein were included for meta-analysis.A random-effects model,standardized mean difference(Hedges’ g),and 95% confidence interval(95%CI) were used for ***:Twenty randomized controlled trials(overall risk of bias:some concerns;GRADE:low to very low) with overweight/obese postmenopausal and older females were *** groups were divided into low-volume RT(LVRT,~44 sets/week) and high-volume RT(HVRT,~77 sets/week).Both RT groups presented improved body adiposity,metabolic risk,and inflammation when compared to ***,HVRT demonstrated higher effect sizes than LVRT for glucose(HVRT=-1.19;95%CI:-1.63 to-0.74;LVRT=-0.78;95%CI:-1.15 to-0.41) and C-reactive protein(HVRT=-1.00;95%CI:-1.32 to-0.67;LVRT=-0.34;95%CI,-0.63 to-0.04)) when compared to ***:Compared to CG,HVRT protocols elicit greater improvements in metabolic risk and inflammation outcomes than LVRT in overweight/obese postmenopausal and older females.

关键词： C-reactive protein Fat mass Lipid profile Menopause Strength training

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Percutaneous nephrostomy versus ureteral stent in hydronephrosis secondary to obstructive urolithiasis:A systematic review and meta-analysis

引用

Asian Journal of Urology 2024年第2期11卷 261-270页

作者： Andreia Cardoso Aparício Coutinho Gonçalo Neto Sara Anacleto Catarina Laranjo Tinoco Nuno Morais Mário Cerqueira-alves Estevão Lima paulo Mota Department of Urology Hospital de BragaBragaPortugal School of Medicine University of MinhoBragaPortugal Association(2CA-Braga).School of Medicine University of MinhoBragaPortugal

Objective: To assess if there is a preferable intervention between retrograde ureteral stent (RUS) and percutaneous nephrostomy (PCN) tube, in cases of upper urinary tract stone obstruction with complications requiring urgent drainage, by evaluating outcomes regarding urinary symptoms, quality of life (QoL), spontaneous stone passage, and length of hospital stays, since there is no literature stating the superiority of one modality over the ***: We searched MEDLINE and other sources for relevant articles in June 2019 without any date restrictions or filters applied. The selection was done first by the title and abstract screening and then by full-text assessment for eligibility. Only randomized controlled trials or cohort studies in patients with hydronephrosis secondary to obstructive urolithiasis that presented comparative data between PCN and RUS placement concerning at least one of the defined outcome measures were included. Lastly, MEDLINE database and PubMed platform were screened again using the same terms, from June 2019 until November ***: Of 556 initial articles, seven were included in this review. Most works were considered of moderate-to-high quality. Three studies regarding QoL showed a tendency against stenting, even though only one demonstrated statistically significant negative impact on overall health state. Two works reported significantly more post-intervention urinary symptoms in stenting patients. One article found that PCN is a significant predictor of spontaneous stone passage, when adjusted for stone size and location. Findings on length of hospital stays were not consistent among ***: PCN appears to be the intervention better tolerated, with less impact on the patient’s perceived QoL and less post-operative urinary symptoms, in comparison with RUS. Nevertheless, further studies with larger samples and a randomized controlled design are suggested.

关键词： Ureteral stent Percutaneous nephrostomy Obstructive urolithiasis Urinary symptoms Quality of life

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Screening of Environmental Bacterial Strains for Wild Aedes Mosquito Larval Biocontrol

引用

Open Journal of Applied Sciences 2024年第9期14卷 2431-2442页

作者： Alexandre paulo Machado Liliana alves Correa Gabriel de Paula Albuquerque Ana Lúcia Maria Ribeiro Rosina Djunko Miyazaki Jéssica da Silva Gava Dyego Bichofre Cruz Laboratory of Microbiology Medical Faculty Federal University of Mato Grosso Mato Grosso Brazil Laboratory of Entomology Institute of Biosciences Federal University of Mato Grosso Mato Grosso Brazil

The effectiveness of current control measures against Aedes mosquitoes remains low, resulting in persistent epidemics in urban areas. The emergence of resistant mosquito populations to chemical insecticides highlights the need for novel, environmentally friendly, cost-effective control strategies. This study explored the potential of environmental bacterial isolates to biocontrol wild Aedes larvae. Initially, we collected bacterial samples from infectious masses of Aedes fluviatilis larvae. The isolated bacteria were identified using biochemical, enzymatic, and molecular methods, including 16S rRNA sequencing and MALDI-TOF. Previously, Aeromonas hydrophila and Bacillus thuringiensis isolated from these infectious masses showed limited Aedes larval inhibition. Consequently, we screened additional environmental isolates from the bacteriotheque. Six isolates previously identified were tested: Chromobacterium violaceum, Enterobacter cloacae, Bacillus cereus, Bacillus sphaericus, and two strains of Bacillus thuringiensis israelensis. Among these strains, B. thuringiensis and C. violaceum exhibited significant inhibitory activities against wild Aedes larvae. Bacillus thuringiensis cultures grown under daylight conditions showed a slight ability to inhibit Aedes larvae. The potential of B. thuringiensis and C. violaceum strains studied, along with optimized culture growth conditions, will be further investigated to develop bioinsecticide products to provide safer and more sustainable alternatives for controlling larvae of Aedes mosquitoes.

关键词： Bacillus thuringiensis Chromobacterium violaceum Aedes Biocontrol Larval Inhibition

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

ARHCS (Automatic Rainfall Half-Life Cluster System): A Landslides Early Warning System (LEWS) Using Cluster Analysis and Automatic Threshold Definition

引用

International Journal of Geosciences 2024年第1期15卷 54-69页

作者： Cassiano Antonio Bortolozo Luana Albertani Pampuch Marcio roberto Magalhães De Andrade Daniel Metodiev Adenilson roberto Carvalho Tatiana Sussel Gonçalves Mendes Tristan Pryer Harideva Marturano Egas Rodolfo Moreda Mendes Isadora Araújo Sousa Jenny Power Cemaden - National Center for Monitoring and Early Warning of Natural Disasters General Coordination of Research and Development S&#227o José dos Campos Brazil Environmental Engineering Department Institute of Science and Technology S&#227o Paulo State University S&#227o José dos Campos Brazil Department of Mathematical Sciences University of Bath Bath UK Institute for Mathematical Innovation University of Bath Bath UK

A significant portion of Landslide Early Warning Systems (LEWS) relies on the definition of operational thresholds and the monitoring of cumulative rainfall for alert issuance. These thresholds can be obtained in various ways, but most often they are based on previous landslide data. This approach introduces several limitations. For instance, there is a requirement for the location to have been previously monitored in some way to have this type of information recorded. Another significant limitation is the need for information regarding the location and timing of incidents. Despite the current ease of obtaining location information (GPS, drone images, etc.), the timing of the event remains challenging to ascertain for a considerable portion of landslide data. Concerning rainfall monitoring, there are multiple ways to consider it, for instance, examining accumulations over various intervals (1 h, 6 h, 24 h, 72 h), as well as in the calculation of effective rainfall, which represents the precipitation that actually infiltrates the soil. However, in the vast majority of cases, both the thresholds and the rain monitoring approach are defined manually and subjectively, relying on the operators’ experience. This makes the process labor-intensive and time-consuming, hindering the establishment of a truly standardized and rapidly scalable methodology on a large scale. In this work, we propose a Landslides Early Warning System (LEWS) based on the concept of rainfall half-life and the determination of thresholds using Cluster Analysis and data inversion. The system is designed to be applied in extensive monitoring networks, such as the one utilized by Cemaden, Brazil’s National Center for Monitoring and Early Warning of Natural Disasters.

关键词： Landslides Early Warning System (LEWS) Cluster Analysis Landslides Brazil

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Remote Sensing Applied to the Evaluation of Spatial and Temporal Variation of Water Quality in a Coastal Environment, Southeast Brazil

引用

Journal of Geographic Information System（地理信息系统） 2019年第5期

作者： Pedro Bettencourt Julio Cesar Wasserman Fábio Ferreira Dias paulo roberto alves Dandara Bernardino Bezerra Camila Américo Santos Luis Perez Zotes Sérgio Ricardo Barros

来源：评论

学校读者我要写书评

暂无评论

Chronic hepatitis C genotype 1 virus:Who should wait for treatment?

引用

World Journal of Gastroenterology 2014年第11期20卷 2867-2875页

作者： Cristiane Valle Tovo Angelo alves de Mattos paulo roberto Lerias de Almeida Department of Gastroenterology Universidade Federal de Ciências da Saúde de Porto AlegrePorto Alegre 90430-080Brasil Department of Gastroenterology Hospital Nossa Senhora da Concei??oPorto Alegre 91350-200Brasil

Elucidation of the natural history of chronic hepatitis C (CHC) and the identification of risk factors for its progression to advanced liver disease have allowed many physicians to recommend deferral treatment (triple therapy) in favour of waiting for new drug availability for patients who are at low risk of progression to significant liver disease. Newer generation drugs are currently under development, and are expected to feature improved efficacy and safety profiles, as well as less complex and shorter duration delivery regimens, compared to the current standards of care. In addition, patients with cirrhosis and prior null responders have a low rate (around 15%) of achieving sustained virological response (SVR) with triple therapy, and physicians must also consider the decision to wait for new treatments in the future for these patients as well. Naïve patients are the most likely to achieve a close to 100% SVR rate; therefore, it may be advisable to recommend that patients with mild to moderate CHC should wait for the newer therapy options. In contrast, patients with advanced fibrosis and cirrhosis will be those with the greatest need for expedited therapeutic intervention. There remains a need, however, for establishing definitive clinical management guidelines to maximize the benefit of waiting for new drugs and minimize risk of side effects and non-response to the current triple therapy.

关键词： Hepatitis C virus Chronic hepatitis C Treatment of hepatitis C Cirrhosis Protease inhibitors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：