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Stable isotopes and body composition in children: History, fundamentals, and clinical applications

Health 2013年第8期5卷 61-68页

作者： Wendell costa Bila Joel Alves Lamounier Andre Everton de Freitas Valmin Ramos silva Sylvia Dias Turani Jose Eduardo Dutra de Oliveira Graduate Program in Health Sciences Federal University of Sao Joao Del Rei-West Campus CenterDivinopolisBrazil Graduate Program in Child and Teenager Health-Medicine School-UFMG Belo Horizonte College of Health and Human Ecology-FASEH VespasianoBrazil Faculty of Medicine School of Sciences of Santa Casa de Misericordia of VitoriaNossa Senhora da Gloria Children’s HospitalVitoriaBrazil Faculty of Medicine Federal University of Sao Joao Del Rei-West Campus CenterDivinopolisBrazil Faculty of Medicine University of Sao Paulo-Ribeirao PretoBrazil

The aim of this study was to gather information on the use of stable isotopes to measure total body water and body composition scan in children. We selected studies in the last 13 years, in addition to classical studies on the subject, indexed in the database PubMed, LILACS, BVS and SciELO. The body composition was characterized by the amount of bone tissue, muscle and adipose tissue, also including the organs as well as levels of body water. Your knowledge becomes increasingly important in light of the changes that occur in the nutritional status of various types of diseases in frameworks, such as diabetes mellitus, protein energy malnutrition, in cases of obesity and metabolic syndrome. The ability to accurately assess body fat mass especially in children is associated with the importance of effective strategies for prevention and treatment of childhood obesity. Historically, in addition to clinical applications, measurements of total body water were used to determine body composition in nutritional studies. To the knowledge of the body composition, the body water can be measured and used by the ingestion of a dose of labeled water. The measured isotope enrichment is a function of the amount of body water. The method of deuterium is particularly interesting for the assessment of body composition in children, due to its characteristics of collection and analysis.

关键词： Body Water Deuterium Body Composition Doubly Labeled Water Obesity Children

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Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

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World Journal of Stem Cells 2015年第6期7卷 956-975页

作者： Jorge Ribeiro Tiago Pereira Ana Rita Caseiro paulo Armada-da-silva Isabel Pires Justina Prada Irina Amorim Sandra Amado Miguel Franca Carolina Goncalves Maria Ascensao Lopes Jose Domingos Santos Dina Morais silva Stefano Geuna Ana Lúcia Luís Ana Colette Maurício Departmento de Clínicas Veterinárias and+UPVet Instituto de Ciencias Biomedicas de Abel Salazar(ICBAS)and Centro de Estudos de Ciencia Animal(CECA)Instituto de CienciasTecnologias e Agroambiente da Universidade do Porto(ICETA)Rua de Jorge Viterbo Ferreira4050-313 PortoPortugal Centro Interdisciplinar de Estudo de Performance Humana(CIPER) Faculdade de Motricidade Humana(FMH)Universidade de Lisboa(UL)Estrada da Costa1499-002 Cruz QuebradaDafundoPortugal CECAV and Departamento de Ciencias Veterinárias Universidade de Trás-os-Montes e Alto Douro(UTAD)5001-801 Vila RealPortugal Departmento de Patologia e de Imunologia Molecular Instituto de Ciencias Biomedicas de Abel SalazarRua de Jorge Viterbo Ferreira4050-313 PortoPortugal Instituto Português de Patologia e Imunologia Molecular da Universidade do Porto(IPATIMUP) Rua Dr.Roberto Frias s/n4200-465 PortoPortugal UIS-IPL:Unidade de Investigacao em Saúde da Escola Superior de Saúde de Leiria Instituto Politécnico de Leiria2411-901 LeiriaPortugal CEMUC Departamento de Engenharia Metalúrgica e MateriaisFaculdade de Engenharia da Universidade do Porto(FEUP)Rua Dr.Roberto Frias4200-465 PortoPortugal Biosckin Molecular and Cell Therapies S.A.Tec MaiaRua Engenheiro Frederico Ulrich 26504470-605 MaiaPortugal Neuroscience Institute of the Cavalieri Ottolenghi Foundation and Department of Clinical and Biological Sciences University of Turin

AIM: To study the therapeutic effect of three tubeguides with electrical conductivity associated to mesenchymal stem cells(MSCs) on neuro-muscular regeneration after ***: Rats with 10-mm gap nerve injury were tested using polyvinyl alcohol(PVA), PVA-carbon nanotubes(CNTs) and MSCs, and PVA-polypyrrole(PPy). The regenerated nerves and tibialis anterior muscles were processed for stereological studies after 20 wk. The functional recovery was assessed serially for gait biomechanical analysis, by extensor postural thrust, sciatic functional index and static sciatic functionalindex(SSI), and by withdrawal reflex latency(WRL). In vitro studies included cytocompatibility, flow cytometry, reverse transcriptase polymerase chain reaction and karyotype analysis of the MSCs. Histopathology of lung, liver, kidneys, and regional lymph nodes ensured the biomaterials biocompatibility. RESULTS: SSI remained negative throughout and independently from treatment. Differences between treted groups in the severity of changes in WRL existed, showing a faster regeneration for PVA-CNTs-MSCs(P < 0.05). At toe-off, less acute ankle joint angles were seen for PVA-CNTs-MSCs group(P = 0.051) suggesting improved ankle muscles function during the push off phase of the gait cycle. In PVA-PPy and PVA-CNTs groups, there was a 25% and 42% increase of average fiber area and a 13% and 21% increase of the "minimal Feret's diameter" respectively. Stereological analysis disclosed a significantly(P < 0.05) increased myelin thickness(M), ratio myelin thickness/axon diameter(M/d) and ratio axon diameter/fiber diameter(d/D; g-ratio) in PVA-CNT-MSCs group(P < 0.05). CONCLUSION: Results revealed that treatment with MSCs and PVA-CNTs tube-guides induced better nerve fiber regeneration. Functional and kinematics analysis revealed positive synergistic effects brought by MSCs and PVA-CNTs. The PVA-CNTs and PVA-PPy are promising scaffolds with electric conductive properties, bio- and cytocompatible th

关键词： Nerve regeneration Neurotmesis Tubeguides Mesenchymal stem cells Carbon nanotubes Functional assessment Gait biomechanical analysis Histomorfometry Polyvinyl alcohol

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The Assessment of Medical Students’ Chronotypes

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World Journal of Neuroscience 2017年第3期7卷 275-281页

作者： José Reinaldo Maximo Gomes Fátima Karine Ferreira Santos Isaac Filipe Melo de Freitas Ilma Ferreira de Oliveira Hete Aguida dos Santos José Cláudio da silva Janise dal Pai Lysien Ivania Zambrano Euclides Mauricio Trindade Filho Milton Vieira costa Laboratório de Neurociência e comportamento Universidade Estadual de Ciências da Saúde de Alagoas—UNCISAL Maceió Brazil Universidade Nove de Julho (UNINOVE) S&atilde o paulo Brazil Facultad de Ciencias Medicas Universidad Nacional Autónoma de Honduras Tegucigalpa Honduras

Objective: This study aimed to investigate the circadian typology of medical students at public universities from one of the northeastern states in Brazil, active in the morning and afternoon shifts, checking the compatibility between their study time, productivity, income and chronotype. Methods: To obtain the results, the assessment of the chronotype in 342 medical students of both sexes was considered. Each participant was submitted to the questionnaire developed by Horne and Ostberg, adapted by Cardinali, Colomberk and Rey. It’s composed of 9 questions, providing the general ranking of each individual. Based upon scored, the chronotypes were classified. Results: The sample was composed of 49.12% (168) male and 50.87% (174) female subjects, a similar gender distribution of enrolled students during data collection time. By applying the questionnaire, the prevalent rating among the medical students as moderately morning (29.82%) and intermediate (43.85%) has been relevant. The academic performance assessment in two morning-intermediate groups (8.16) had no significant distance from others considered afternoon active (8.20). Conclusion: The health sciences student, especially Medicine, faces constant adaptation of scheduling, in the attempt of fulfilling the requirements throughout the course, causing chronogram desynchronization effects and forced readaptation to each different school semester.

关键词： Circadian Rhythm Chronobiology Disorders Delivery of Health Care Medicine Sleep Disorders

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Galvanic and asymmetry effects on the local electrochemical behavior of the 2098-T351 alloy welded by friction stir welding

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Journal of Materials Science & Technology 2020年第10期42卷 162-175页

作者： Mariana X.Milagre Uyime Donatus Naga V.Mogili Rejane Maria Psilva Barbara Victoria Gde Viveiros Victor F.Pereira Renato A.Antunes Caruline S.CMachado Joao Victor S.Araujo Isolda costa Instituto de Pesquisas Energeticas e Nucleares IPEN/CNENAv.Prof.Lineu Prestes2242Sao PauloBrazil Laboratorio Nacional de Nanotecnologia LNNano/CNPENRua Giuseppe Maximo Scolfaro10.000Polo II deAlta Tecnologia de CampinasBrazil Centro de Engenharia Modelagem e Ciencias Sociais AplicadasUFABCAv.dos Estados 5001Santo AndreBrazil

Scanning electrochemical microscopy(SECM)and scanning vibrating electrode technique(SVET)were used to investigate the electrochemical behaviour of the top surface of the 2098-T351 alloy welded by friction stir welding(FSW).The SVET technique was efficient in identifying the cathodic and anodic weld *** welding joint(WJ),which comprises the thermomechanically affected zone(TMAZ)and the stir zone(SZ),was cathodic relative to the heated affected zone(HAZ)and the base metal(BM).The reactivities of the welding joint at the advancing side(AS)and the retreating side(RS)were analyzed and compared using SECM technique in the competition mode by monitoring the dissolved oxygen as a redox mediator in 0.005 mol L^-1 NaCl *** RS was more electrochemically active than the AS,and these results were correlated with the microstructural features of the welded alloy.

关键词： Aluminum alloys Friction stir welding Localized corrosion

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Effects of Probiotic Lactobacillus gasseri Strains of Breast-Fed Infant in a Murine Model

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Journal of Food Science and Engineering 2014年第6期4卷 271-281页

作者： Maria da Penha Piccolo Ramos Joao Alexandre Tres Pancoto Iracilda Zeppone Carlos Rogeria Comastri deCastro Almeida danielle Cardoso Geraldo Maia Livia Carolina de Abreu Ribeiro Femanda Paulin Benzatti Luciana Rodrigues Cunha Aline costa e silva Celia Lucia de Luces Fortes Ferreira Departamento de Ciencias da Saude Universidade Federal do Espirito Santo S6o Mateus Espirito Santo CEP. 29.932-540 Brazil Laboratorio de Imunonologia Clinica Universidade Estadual Paulista Faculdade de Ciencias Farmaceuticas Araraquara Sao Paulo CEP: 14.801-902 Brazil Departamento de Ciencia dos Alimentos Universidade Federal da Bahia Eseola de Nutricao Salvador Bahia CEP. 40.110-160 Brazil Departamento de Tecnologia de Alimentos Universidade Federal de Ouro Preto Ouro Preto Minas Gerais CEP: 35.400-970 Brazil Departamento de Tecnologia de Alimentos Universidade Federal de Vigosa Vigosa Minas Gerais CEP: 36.570-000 Brazil

The ingestion of probiotic lactic acid bacteria has been evaluated and noted that it has an effect on the balance of desirable microbiota in the gastrointestinal tract. Lactobacillus gasseri demonstrates good survival in the gastrointestinal tract, and it has been associated with a variety of probiotic activities and roles, including the reduction of fecal mutagenic enzymes, the production of bacteriocins and the stimulation of macrophages immunomodulation. The aim of the study was to evaluate the effects of a pool of L. gasseri strains isolated from the feces of breastfed infants added in the human milk of healthy women. The milk was both pasteurized and unpasteurized, to verify the cell cytotoxicity of macrophages and to quantify the production of immunologic mediators such as IL-4, IL-6, IFN-γ, TNF-α, NO and oxygen intermediary compounds （H2O2）. The administration of raw human milk and pasteurized human milk to infants is a regular, encouraged practice in units of intensive therapy （UITs） and our present investigation verified the beneficial effect of addition of a pool of L. gasseri to pasteurized human milk （PHML）. Our results show that probiotic supplementation helped to maintain cell viability, reduced IL-6 and IFN-γ production and stimulated TNF-α, NO, H2O2, IL-4 production. Nevertheless, the results indicate that the addition of lactobacillus to human milk was not a determinant in the production of TNF-α. L. gasseri added to breast milk did not present a cytotoxic risk, and the addition ofL. gasseri to pasteurized milk of human milk bank would benefit newborns that depend on milk banks for the colonization of more desirable microbiota.

关键词： Probiotic human milk bank immunology Lactobacillus gasseri.

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氮素改变辣椒初级代谢和薄壁组织厚度调控比叶面积研究

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辣椒杂志 2021年第3期19卷 36-48页

作者： Lucas de ávila silva Rebeca P.Omena-Garcia Jorge A.Condori-Apfata paulo Mafra de Almeida costa Natália Machado silva Fábio M.da Matta Agustin Zsogon Wagner L.Araújo Edgard A.de Toledo Picoli Ronan Sulpice Adriano Nunes-Nesi 沈凌峰吉雪花(译) 不详石河子大学农学院乌鲁木齐832003

比叶面积(SLA)是影响植物光截获和光利用效率的关键性状,常常影响植物的生长和产量。SLA是解释不同植物在不同环境下生长差异的一个关键性状。光照和氮素供应都是决定SLA的重要因素。为了更好地了解辐照度和氮素对辣椒(Capsicum chinens... 详细信息

比叶面积(SLA)是影响植物光截获和光利用效率的关键性状,常常影响植物的生长和产量。SLA是解释不同植物在不同环境下生长差异的一个关键性状。光照和氮素供应都是决定SLA的重要因素。为了更好地了解辐照度和氮素对辣椒(Capsicum chinense)SLA的影响,研究对两个中国辣椒商品种(Biquinho和Habanero)的初级代谢产物和形态特征进行了分析。两种基因型辣椒的SLA随着遮阴而增加,但随氮供应量的增加而下降;在氮素供应由缺乏到充足的范围内Habanero品种的SLA表现稳定。相关分析表明,高氮供应导致SLA降低是因为氨基酸、蛋白质和淀粉含量的调节,从而影响叶片密度。此外,在中度缺氮到充足供氮范围内,两种基因型辣椒的SLA表现出响应差异,Biquinho和Habanero分别在栅栏和海绵状薄壁组织上表现出差异。总之,结果表明,SLA对氮供应的反应是由某些代谢物含量和依赖于基因型的影响叶片厚度及密度的薄壁组织变化平衡调节的。

关键词：辣椒叶片形态和解剖结构组织密度遮阴

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Concentrations of Silver Nitrate in the In Vitro Development and Conservation of Passiflora gibertii ***

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American Journal of Plant Sciences 2017年第12期8卷 2944-2955页

作者： Gláucia Amorim Faria Lucas Menezes Felizardo Antonio Flávio Arruda Ferreira Paula Suares Rocha Aline Namie Suzuki Antonio da silva Souza Tatiana Góes Junghans Maria Angélica Pereira de Carvalho costa Ana Patricia Bastos Peixoto Augusto Ramalho de Morais Beatriz Garcia Lopes Tiago Almeida de Oliveira Departmentof Mathematics School of EngineeringSao Paulo State University(Unesp)Ilha SolteiraBrazil Department of Civil Engineering School of EngineeringSao Paulo State University(Unesp)Ilha SolteiraBrazil Laboratory of Environmental Mutagenesis Institute of BiosciencesSao Paulo State University(Unesp)Rio ClaroBrazil Embrapa Cassava and Fruticulture Cruz das AlmasBrazil Center for Agrarian Biological and Environmental SciencesFederal University of the Recôncavo of Bahia(UFRB)Cruz das AlmasBrazil Department of Statistics State University of Paraíba(UEPB)Campina GrandeBrazil Department of Exact Sciences Federal University of Lavras(UFLA)LavrasBrazil

The aim of this study was to evaluate the effects of silver nitrate in the in vitro development and conservation of passion fruit plants. Experiments were carried out at the Laboratory of Culture and Tissues, in the National Cassava and Fruits Research Center (EMBRAPA), using microcuttings of Passiflora gibertii N. E. Br., previously cultivated in vitro. The microcuttings were placed in MS media supplemented with silver nitrate at concentrations of 0, 1, 2, 4 and 8 mg·L-1, and, during 30, 60 and 90 days, the following variables were identified: Shooting length (cm), number of roots, number and coloration of leaves. A completely randomized design with 30 replications in a split-plot scheme was employed. Polynomial regression equations were adjusted in analysis of variance to compare averages of treatments. The obtained results support the assumption of high sensitivity of passion fruit tissues to ethylene, which reflects in the lost of vigor and in the induction of culture senescence by its accumulation. Based on obtained results, and in order to mitigate this problem, it is possible to indicate an addition of 2 mg·L-1?of silver nitrate in culture media, for the micropropagation of passion fruit plant, allowing the controlling of the atmosphere inside the culture test tubes, during the establishment of explants and other steps of the process.

关键词： Passion Fruits Tropical Fruits Plant Propagation Reduced Growth Ethylene

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Protective Properties in Hymenaea martiana Hayne against Multi-drug-resistant Staphylococcus aureus

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Veterinary Science Research 2020年第2期2卷 16-23页

作者： Dielson da silva Vieira Tânia Maria Sarmento da silva Timothy AHackett Marianade Barros Weslen Fabrício Pires Teixeira Juliana Campos Pereira Diniz Pedro HenriqueGorni Sanely Lourenço da costa Maria Aparecida Scatamburlo Moreira MateusMatiuzzi da costa Francisco Leydson Formiga Feitosa São paulo State University“Júlio de Mesquita Filho”(UNESP) School of Veterinary MedicineAraçatubaSao PauloBrazil Federal Rural University of Pernambuco(UFRPE) Department of Molecular SciencesRecifePernambucoBrazil Department of Biochemistry University of Nebraska-LincolnLincolnNE68508USA Federal University of Viçosa(UFV) Veterinary DepartmentViçosaMinas GeraisBrazil Federal University of Vale do São Francisco(UNIVASF) Department of Animal SciencePetrolinaPernambucoBrazil University of West Paulista(UNOESTE) Department of AgronomyPresidente PrudenteSao PauloBrazil Federal University of Goiás(UFG) Department of Veterinary MedicineGoiâniaGoiásBrazil

Antibiotic resistance represents a widespread problem in milk *** identification of compounds for a topically applied ointment used in mastitis therapy remains *** from the genus Hymenaea can be administered in cases of multi-drugresistant Staphylococcus aureus infection for ruminant species,but the protective properties are not well *** this research the aim is verify the protective effects of *** against *** infection in bovine mammary epithelial cell line(MAC-T)and to obtain an antioxidant profile evaluation in *** MAC-T cells were challenged with *** after being exposed to the extract of the *** in the protective *** the verification of the viability of the MAC-T cells,the MTT assay was performed,and was used dilutions of the plant extract,starting at 2.5%.The extract of *** was evaluated for antioxidant aspect in different dilutions by FRAP,ORAC and DPPH.A variety of flavonoids(quercetin,luteolin,etc.)have been identified as the main components by using mass spectrometry,reinforcing our in vitro findings that flavonoids,especially quercetin,have a medicinal profile capable of killing mastitis-causing *** excellent antioxidant pattern was observed in the 2.5%solution;however,membrane integrity in MAC-T cells was *** findings suggest low dilutions of *** extract has a desirable protective effect from *** *** in vitro studies can be gleaned upon for further in vivo studies.

关键词： dairy animal Fitoterapic Hymenaea gender Cell activity Mastitis

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Three-dimensional imaging of the uterus:The value of the coronal plane

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World Journal of Radiology 2015年第12期7卷 484-493页

作者： Lufee Wong Nikki White Jayshree Ramkrishna Edward Araujo Júnior Simon Meagher Fabricio da silva costa Monash Ultrasound for Women Department of Obstetrics and Gynaecology Monash Medical CentreSouthern Health Department of Obstetrics Paulista School of Medicine-Federal University of Sao Paulo(EPM-UNIFESP)

Advent in three-dimensional(3D) imaging technology has seen 3D ultrasound establish itself as a useful adjunct complementary to traditional two-dimensional imaging of the female pelvis. This advantage largely arises from its ability to reconstruct the coronal plane of the uterus, which allows further delineation of many gynecological disorders. 3D imaging of the uterus is now the preferred imaging modality for assessing congenital uterine anomalies and intrauterine device localization. Newer indications include the diagnosis of adenomyosis. It can also add invaluable information to delineate other endometrial and myometrial pathology such as fibroids and endometrial polyps.

关键词： Three-dimensional ultrasound Coronal view Pelvis Uterus Uterine anomalies

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Prevalence of Frailty Syndrome in the Elderly and Associated Factors in Brazil

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Health 2015年第11期7卷 1591-1599页

作者： Anna Ferla Monteiro silva Passos Iris do Céu Clara costa Fábia Barbosa de Andrade Maria do Carmo Eulálio Anita Liberalesso Neri Romulo Lustosa Pimenteira de Melo Adrianna Ribeiro Lacerda Postgraduation Program in Health Sciences Federal University of Rio Grande do Norte Natal Brazil Department of Dentistry Federal University of Rio Grande do Norte Natal Brazil Nursing Faculty of Health Sciencies of Trairi/Federal University of Rio Grande do Norte Santa Cruz Brazil Department of Psychology State University of Paraíba Campina Grande Brazil Department of Educational Psychology State University of Campinas Campinas Brazil

This paper aims to identify the prevalence of frailty syndrome and its association with demographic, economic, health, psychological and functional variables in Brazilian population. The study was cross-sectional and composed of 385 elderly aged from 65 years, an average age of 73.92 years. A multivariate Poisson regression was used to check for conditions associated with frailty and to determine the prevalence (α = 0.05). The prevalence of frailty was 8.7% and pre-frailty of 50.4%. The frail and pre-frail older adults showed larger and increasing prevalence ratios for marital status, difficulty performing instrumental activities of daily living, old age, involuntary loss of feces, depression and negative affections. These results can guide the establishment of preventive measures and the development of intervention strategies aimed at minimizing the adverse effects of frailty in elderly people.

关键词： Aging Frail Elderly Frailty Frailty Syndrome

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