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Leptin gene polymorphism a昀ects leptin level in childhood asthma

World Journal of Pediatrics 2018年第6期14卷 601-606页

作者： Dawid Szczepankiewicz paulina sobkowiak Beata Naro(z)na Irena Wojsyk-Banaszak Anna Br(e)borowicz Aleksandra Szczepankiewicz Department of Animal Physiology and Biochemistry Poznan University of Life SciencesPoznanPoland Department of Pediatric Pulmonology Allergy and Clinical ImmunologyIIIrd Department of PediatricsPoznan University of Medical SciencesPoznanPoland Laboratory of Molecular and Cell Biology Department of Pediatric PulmonologyAllergy and Clinical ImmunologyIIIrd Department of PediatricsPoznan University of Medical SciencesSzpitalna St.27/3360-572 P oznanPoland

Background Leptin may induce inflammation in asthma by activation of Th2 cells. It has also been demonstrated that leptin expression increases upon inflammation and that asthmatic patients show increased serum leptin levels. We hypothesized that the polymorphism in leptin (LEP) and leptin receptor (LEPR) genes is associated with childhood asthma and may affect their serum level. To our knowledge, there are no reports analyzing LEP and LEPR polymorphisms in association with their serum levels in childhood asthma. Methods We analyzed 35 subjects: 25 asthmatic pediatric patients and 10 healthy children aged from 6 to 18. The diagnosis of allergic asthma was based on clinical manifestation, lung function, positive skin prick tests and increased immunoglobulin E levels. The polymorphisms were genotyped with use of polymerase chain reaction-restriction fragment length polymor-phism method. Serum levels of leptin and leptin receptor were determined using BioVendor enzyme-linked immunosorbent assay kits. Statistical analysis was done with Statistica v.12. Results We observed that leptin levels were increased in asthmatic subjects as compared to healthy controls and were sig-nificantly higher during exacerbation than in the asymptomatic period (P=0.025). We observed that LEP polymorphism (rs13228377) was associated with higher serum leptin levels in asthma and these two variables had high predictive value for asthma risk (P=0.007, odds ratio 17.5, predictive accuracy 83.9%). LEPR polymorphisms did not show association with its serum level and asthma risk. Conclusion LEP polymorphism may increase asthma risk via influence on its serum level.

关键词： Asthma Gene Leptin Leptin receptor Polymorphism

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