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Oxidative metabolism of photosynthetic species and the exposure to some freshwater and marine biotoxins

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BIOCELL 2021年第4期45卷 813-821页

作者： SUSANA PUNTARULO paula mARIELA GONZÁLEZ Universidad de Buenos Aires Facultad de Farmacia y BioquímicaFisicoquímicaBuenos AiresCP 1113Argentina CONICET-Universidad de Buenos Aires.Instituto de Bioquímica y medicina molecular(IBImOL) Buenos AiresCP 1113Argentina

Environmental climate conditions could lead to an increasing global occurrence of microorganism blooms that synthesize toxins in the aquatic *** blooms could result in significantly toxic *** of photosynthetic organisms to adverse environmental conditions implicate reactive oxygen species generation;but,due to the presence of a varied cellular antioxidant defense system and complex signaling networks,this oxidative stress could act as an important factor in the environmental adaptive *** objective of this review was to assess how some biotoxins are implicated in the generation of oxidative and nitrosative metabolic changes,not only in biotoxin-producing organisms but also in non-producing ***,toxins may modify the oxidative cellular balance of several other ***,the effect of toxins on the oxidative and nitrosative conditions will be evaluated in freshwater and marine algae and vascular *** changing climate conditions could act as agents capable of modifying the community composition leading to alterations in the global health of the habitat,risking the survival of many species with ecological relevance.

关键词： Blooms Climate change metabolic stress Photosynthetic organisms Toxins

Flower heads in Asteraceae—recruitment of conserved developmental regulators to control the flower-like inflorescence architecture

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Horticulture Research 2018年第1期5卷 491-500页

作者： paula Elomaa Yafei Zhao Teng Zhang Department of Agricultural Sciences Viikki Plant Science CentreUniversity of HelsinkiP.O.Box 2700014HelsinkiFinland

Inflorescences in the Asteraceae plant family,flower heads,or capitula,mimic single flowers but are highly compressed structures composed of multiple *** transference of a flower-like appearance into an inflorescence level is considered as the key innovation for the rapid tribal radiation of *** molecular data indicate that Asteraceae flower heads resemble single flowers not only morphologically but also at molecular *** summarize this data giving examples of how rewiring of conserved floral regulators have led to evolution of morphological innovations in *** diversification of the highly conserved flower meristem identity regulator LEAFY has shown a major role in the evolution of the capitulum ***,gene duplication and subsequent sub-and neofunctionalization of SEPALLATA-and CYCLOIDEA-like genes in Asteraceae have been shown to contribute to meristem determinacy,as well as flower type differentiation—key traits that specify this large *** challenge is to integrate genomic,as well as evolutionary developmental studies in a wider selection of Asteraceae species to understand the detailed gene regulatory networks behind the elaborate inflorescence architecture,and to promote our understanding of how changes in regulatory mechanisms shape development.

关键词： flowers architecture head

Doxycycline induces bone repair and changes in Wnt signalling

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International Journal of Oral Science 2017年第3期9卷 158-166页

作者： kátia do nascimento gomes ana paula negreiros nunes alves paula góes pinheiro dutra glauce socorro de barros viana faculty of dentistry pharmacy and nursingfederal university of cearáFortalezaBrazil faculty of medicine federal university of cearáFortaleza Brazil

Doxycycline （DOX） exhibits anti-inflammatory and mmP inhibitory properties. The objectives of this study were to evaluate the effects of DOX on alveolar bone repair. Controls （CTL） and DOX-treated （10 and 25 mg. kg- 1） molars were extracted, and rats were killed 7 or 14 days later. The maxillae were processed and subjected to histological and immunohistochemical assays. Hematoxylin-eosin staining （7th day） revealed inflammation in the CTL group that was partly reversed after DOX treatment. On the 14th day, the CTL group exhibited bone neoformation, conjunctive tissue, re-epithelization and the absence of inflammatory infiltrate. DOX-treated groups exhibited complete re-epithelization, tissue remodelling and almost no inflammation. Picrosirius red staining in the DOXlO group （7th and 14th days） revealed an increased percentage of type I and III collagen fibres compared with the CTL and DOX25 groups. The DOXlO and DOX25 groups exhibited increases in osteoblasts on the 7th and 14th days. However, there were fewer osteoclasts in the DOXlO and DOX25 groups on the 7th and 14th days. Wnt-lOb- immunopositive cells increased by 130% and 150% on the 7th and 14th days, respectively, in DOX-treated groups compared with the CTL group. On the 7th day, Dickkopf （Dkk）-I immunostaining was decreased by 63% and 46% in the DOXlO and DOX25 groups, respectively. On the 14th day, 69% and 42% decreases in immunopositive cells were observed in the DOXlO and DOX25 groups, respectively, compared with the CTL group. By increasing osteoblasts, decreasing osteoclasts, activating Wnt lOb and neutralising Dkk, DOX is a potential candidate for bone repair in periodontal diseases.

关键词： alveolar bone loss bone diseases bone repair doxycycline Dickkopf-1 immunohistochemistry Wnt-lOb

Programmed cell death in seeds of angiosperms

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Journal of Integrative Plant Biology 2015年第12期57卷 996-1002页

作者： maría paula López-Fernández Sara maldonado Laboratory of Plant Development Department of Biodiversity and Experimental Biology Faculty of Exact and Natural Sciences University of Buenos Aires National Research Council of Argentine (CONICET)

During the diversification of angiosperms, seeds have evolved structuralp chemical, molecular and physiolog- ically developing changes that specially affect the nucellus and endosperm. All through seed evolution, programmed celldeath （PCD） has played a fundamental role. However, examples of PCD during seed development are limited. The present review examines PCD in integuments, nucellus, suspensor and endosperm in those representative examples of seeds studied to date.

关键词： Caspases endosperm KDEL-CysEP metacaspases nucellus nucleases programmed cell death perisperm ricinosomes

Effect of Poly(ε-caprolactone-b-tetrahydrofuran)Triblock Copolymer Concentration on morphological,Thermal and mechanical Properties of Immiscible PLA/PCL Blends

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Journal of Renewable materials 2019年第2期7卷 129-138页

作者： paula do Patrocínio Dias marcelo Aparecido Chinelatto Department of materials Engineering-Campus II São Carlos School of Engineering-EESCUniversity of São Paulo-USPAv.João Dagnone1100-Jd.Sta AngelinaSão Carlos-SP-Brazil

In this study a low molecular weight triblock copolymer derived fromε-caprolactone and tetrahydrofuran was used as a non-reactive compatibilizer of immiscible PLA/PCL *** blends with 0,1.5 wt%,3 wt%and 5 wt% copolymer and about 75 wt%PLA were prepared by single screw extrusion and characterized by scanning electron microscopy(SEm),differential scanning calorimetry(DSC),dynamic mechanical analysis(DmA),tensile and Izod impact *** micrographs showed that the size of the dispersed PCL domains was practically constant regardless of copolymer *** result can be explained by the low shear rate employed during processing step and a decrease of PCL viscosity by presence of the triblock ***,when the copolymer concentration increased,strain at break of PLA/PCL blends also ***/PCL blend with 0 wt% copolymer presented 2%strain at break,whereas PLA/PCL blend with 5 wt%copolymer exhibited 90%.

关键词： Blends non-reactive compatibilization poly(lactic acid) poly(ε-caprolactone) triblock copolymer

Assisted Natural Remediation of a Trace Element-Contaminated Acid Soil:An Eight-Year Field Study

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Pedosphere 2015年第2期25卷 250-262页

作者： Jian XIONG paula mADEJON Engracia mADEJON Francisco CABRERA Instituto de Recursos Naturales y Agrobiologia de SevillaIRNAS CSICAvenida Reina Mercedes10P.O.Box 1052Seville 41080 Spain

There are many remediation techniques for organic contaminated soils,but relatively few of them are applicable to trace elementcontaminated soils.A field experiment was carried out to investigate assisted natural remediation(ANR) of an acid soil contaminated by As,Cd,Cu,Zn and Pb using one inorganic amendment,sugar beet lime(SL),and two organic amendments,biosolid compost(BC)and leonardeite(LE).The experiment was arranged in a completely randomized block design with four treatments in three replicates:1) a non-amended control(NA);2) SL amended at 30 mg ha^(-1) year^(-1);3) BC amended at 30 mg ha^(-1) year^(-1) and 4) LE amended at 20 mg ha^(-1) year^(-1) plus SL amended at 10 mg ha^(-1) year^(-1)(LESL).The amended plots received two doses of each amendment(DO2):one in October 2002 and another in October *** plots were then divided in half into two subpolts and one subplot received another two doses of the same amendments(DO4) in October 2005 and October *** 2011,the pH values of the amended soils were greater than that of the NA soil,with the SL-amended soil showing the highest *** organic carbon(TOC) was also greater in the amended soil,and greater with DO4 than with *** reduced the concentrations of 0.01 mol L^(-1) CaCl_2-extractable Cd,Cu and Zn,especially in the SL-amended *** cover of colonizing vegetation was enhanced by amendments,but was independent of amendment *** in soil properties from 2003 to 2011 showed that the first amendment application of DO2 caused a high differentiation between all the amendment treatments and the NA *** the second application of DO2,soil pH and TOC continued increasing *** two applications of amendments(DO4) caused differences only in the organic *** cover increased over time in all the treatments including *** could be concluded that the slow and steady natural remediation of this soil could be enhanced by amendment application(ANR).

关键词： amendment application CaCl2 extraction colonizing vegetation plant cover soil contamination soil property

Recent advances in magnetic electrospun nanofibers for cancer theranostics application

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Progress in Natural Science:materials International 2021年第6期31卷 835-844页

作者： paula I.P.Soares Jo?o Paulo Borges CENImAT/i3N Departamento de Ciência dos Materiais Faculdade de Ciências e Tecnologia Universidade Nova de Lisboa

Cancer theranostics is a recent concept that aims to combine in the same device diagnostic and therapeutic features. magnetic nanoparticles(m NPs) are commonly used as a critical part of these systems due to their ability to respond to an external magnetic field. Consequently, m NPs can generate heat when an alternating magnetic field is applied and enhance image contrast in magnetic resonance. However, direct administration of m NPs intravenously or directly in the tumor can lead to undesired side effects because of m NP elimination by macrophages or leakage to healthy tissues. Therefore, m NPs can be retained in a polymeric nanofibrous mesh, thus preventing misplacing or loss of m NPs. Furthermore, these magnetic nanofibers can be directly implanted in the tumor site, thus ensuring high m NPs loading and higher magnetic response. In addition, polymeric nanofibers produced by electrospinning are frequently used to maintain a sustained drug release in the tumor site. Therefore,a magnetic polymeric nanofiber produced by electrospinning is an ideal nanosystem for cancer theranostics application. This review summarizes the most recent developments of magnetic nanofibers produced by electrospinning for cancer theranostics applications.

关键词： Electrospinning Nanofibers Iron oxide nanoparticles magnetic fluid hyperthermia Cancer theranostics

UV-B Radiation Induces mu Element Somatic Transposition in maize

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molecular Plant 2013年第6期6卷 2004-2007页

作者： Julia Questa Virginia Walbot paula Casati Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI) Universidad Nacional de Rosario Rosario Argentina Cell and Developmental Biology John Innes Centre Norwich Research Park UK Department of Biology Stanford University Stanford CA 94305-5020 USA

Dear Editor, The maize mutator （muDRImu） transposon family is the most active DNA transposon in plants （Lisch, 2002）. The mu family contains diverse elements, all sharing similar -215-bp terminal inverted repeats （TIRs）. muDR is the autonomous element, and a transcriptionally active muDR is required for transposition of the non-autonomous mu elements （Chomet et al., 1991）. muDR contains two genes, mudrA and mudrB （Figure 1A）; mudrA encodes the mURA transposase, and mudrB encodes a protein with unknown function. To avoid the deleterious effects of transposons, plants have acquired mechanisms to epigenetically silence them. Silenced mu ele- ments become heavily methylated, and their reactivation is very rare. To date, only UV-B radiation treatments have reac- tivated silenced mu （Walbot, 1999）.

关键词： UV-B 元素辐射诱导玉米换位表观遗传学转座子赋值函数

Risk Governance,Assessment,and Economic Impacts

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International Journal of Disaster Risk Science 2017年第3期8卷 235-236页

作者： paula Teves-Costa Josémanuel mendes Instituto Dom Luiz Faculdade de Ciências Universidade de Lisboa European Center on Urban Risks (CERU) Center for Social Studies Faculty of Economics University of Coimbra

Due to climate change,growing urbanization,and population migration the social and economic impacts of natural hazards have been increasing *** challenge for risk science,both methodologically and conceptually,is to i... 详细信息

关键词： Risk Governance,Assessment,and Economic Impacts