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Native and exotic seed dispersal by the stone marten(Martes foina):implications for the regeneration of a relict climactic forest in central Portugal

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Integrative Zoology 2019年第3期14卷 280-292页

作者： Lúcia pereira Ana VASQUES paula MAIA Maria João RAMOS pereira Carlos FONSECA Milena MATOS CESAM-Centre for Environmental and Marine Studies-Wildlife Research Unit Department of BiologyUniversity of AveiroAveiroPortugal CESAM-Centre for Environmental and Marine Studies-Department of Environment and Planning University of AveiroAveiroPortugal Erasmus University College Life Sciences DepartmentRotterdamthe Netherlands Institute of Biosciences PPGBANDepartment of ZoologyUniversidade Federal do Rio Grande do SulBrazil BioLiving Association FrossosAlbergaria-a-VelhaPortugal

The stone marten(Martes foina)is a carnivorous mammal that often consumes fleshy fruits,thus potentially promoting seed *** present study was developed in Bussaco National Forest,central Portugal,and aimed to assess the potential role of the stone marten in dispersing native and exotic plants in different forest landscape *** from stone marten scats and fleshy fruits were collected monthly and were thereafter identified and sowed in a nursery,following a randomized experimental *** emergence was monitored *** linear models were used to test for differences in time and success of emergence between seeds from scats and fruits of 3 native species(Rubus ulmifolius,Arbutus unedo and Celtis australis)and 1 exotic plant species with invasive behavior(Prunus laurocerasus).Fruit consumption by the stone martens significantly increased and accelerated the germination of the native *** but had no effect on the other 2 native species or on the invasive *** suggested that stone martens contribute to gene flow and forest regeneration by dispersing native plant ***,although the germination was not enhanced in the invasive species,the preference of stone martens for these fruits may potentially contribute to the proliferation of *** study represents a contribution to better understanding the fauna and flora interactions,enabling for a more conscious and effective decision-making in forest management.

关键词： germination invasive species Martes foina Prunus laurocerasus relict climactic forest

Genetic testing vs microforceps biopsy in pancreatic cysts:Systematic review and meta-analysis

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World Journal of Gastroenterology 2019年第26期25卷 3450-3467页

作者： Sandra Faias Luisa pereira Angelo Luis paula Chaves Marilia Cravo Department of Gastroenterology Instituto Portugues de Oncologia de Lisboa de Francisco GentilEPELisboa 1099-023Portugal Faculdade de Ciencias da Seude Universidade da Beira InteriorCovilha 200-506Portugal GRUBI-Grupo de Revisoes Sistematicas Universidade da Beira InteriorCovilha 6200-506Portugal Centro de Matemática e Aplicacoes(CMA-UBI) Universidade da Beira InteriorCovilha 6200-506Portugal Centro de Investigacao em Ciencias da Saude(CICS-UBI) Universidade da Beira InteriorCovilha 6200-506Portugal Department of Pathology Instituto Portugues de Oncologia de Lisboa de Francisco GentilEPELisboa 1099-023Portugal Department of Gastroenterology Hospital Beatriz AngeloLoures 2674-514Portugal Faculdade de Medicina Universidade de LisboaLisboa 1099-023Portugal

BACKGROUND Carcinoembryonic antigen(CEA)and cytology in pancreatic cystic fluid are suboptimal for evaluation of pancreatic cystic *** testing and microforceps biopsy are promising tools for pre-operative diagnostic improvement but comparative performance of both methods is *** To compare the accuracy of genetic testing and microforceps biopsy in pancreatic cysts referred for *** We performed a literature search in Medline,Scopus,and Web of Science for studies evaluating genetic testing of cystic fluid and microforceps biopsy of pancreatic cysts,with endoscopic ultrasound with fine-needle aspiration(EUSFNA)prior to surgery and surgical pathology as reference standard for *** evaluated the diagnostic accuracy for:1-benign cysts;2-mucinous low-risk cysts;3-high-risk cysts,and the diagnostic yield and rate of correctly identified cysts with microforceps biopsy and molecular *** also assessed publication bias,heterogeneity,and study *** Eight studies,including 1206 patients,of which 203(17%)referred for surgery who met the inclusion criteria were analyzed in the systematic review,and seven studies were included in the *** testing and microforceps biopsies were identical for diagnosis of benign *** analysis was superior for diagnosis of both low and high-risk mucinous cysts,with sensitivities of 0.89(95%CI:0.79-0.95)and 0.57(95%CI:0.42-0.71),specificities of 0.88(95%CI:0.75-0.95)and 0.88(95%CI:0.80-0.93)and AUC of 0.9555 and 0.92,*** diagnostic yield was higher in microforceps biopsies than in genetic analysis(0.73 vs 0.54,respectively)but the rates of correctly identified cysts were identical(0.73 with 95%CI:0.62-0.82 vs 0.71 with 95%CI:0.49-0.86,respectively).CONCLUSION Genetic testing and microforceps biopsies are useful second tests,with identical results in benign pancreatic *** analysis performs better for low-and high-risk cysts but has lower diagnos

关键词： Pancreatic cysts Endoscopic ultrasound Endoscopic ultrasound with fineneedle aspiration Genetic testing Microforceps biopsy Molecular analysis KRAS Carcinoembryonic antigen Cytology

Methylation changes at the GNAS imprinted locus in pancreatic cystic neoplasms are important for the diagnosis of malignant cysts

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World Journal of Gastrointestinal Oncology 2020年第9期12卷 1056-1064页

作者： Sandra Faias Marlene Duarte Luísa pereira paula Chaves Marília Cravo Antonio Dias pereira Cristina Albuquerque Department of Gastroenterology Instituto Português de Oncologia de Lisboa Francisco GentilEPELisboa 1099-023Portugal Faculty of Health Sciences University of Beira InteriorCovilhã6200-506Portugal Unidade de Investigação em Patobiologia Molecular(UIPM) Instituto Português de Oncologia de Lisboa Francisco GentilEPELisboa 1099-023Portugal Centro de Matemática e Aplicações(CMA-UBI) Universidade da Beira InteriorCovilhã6200-506Portugal Department of Pathology Instituto Português de Oncologia de Lisboa Francisco GentilEPELisboa 1099-023Portugal Department of Gastroenterology Hospital BeatrizÂngeloLoures 2674-514Portugal

BACKGROUND Guanine nucleotide-binding protein,alpha stimulating(GNAS)mutations are characteristic of intraductal papillary mucinous neoplasms(IPMNs).Pancreatic ductal adenocarcinomas(PDACs)harboring GNAS mutations originate in *** is a complex imprinted locus that produces five transcripts regulated by differential methylated regions,NESP55,GNASAS,GNASXL,GNAS1A,and *** To evaluate if methylation changes in the differential methylated regions of GNAS locus contributed to malignant progression of pancreatic *** GNAS locus methylation was analyzed in archival pancreatic cyst fluid(PCF)obtained by endoscopic ultrasound with fine-needle aspiration by methylation specific–multiplex ligation dependent probe *** were normalized and analyzed using *** *** Fifty-two PCF samples obtained by endoscopic ultrasound with fine-needle aspiration and previously characterized for KRAS and GNAS mutations were *** final diagnoses were surgical(11)and clinicopathological(41),including 30 benign cysts,14 pre-malignant cyst,and eight malignant *** changes at NESP55,GNASAS,GNAS1A,and especially GNASXL were more frequent in malignant cysts,and NESP55 and GNASAS were useful for diagnosis.A combined variable defined as“GNAS locus methylation changes”was significantly associated with malignancy(6/8 malignant cysts and only 2/20 benign cysts)and improved *** in both maternally(NESP55)and paternally(GNASXL)derived promoters was found in 3/3 *** This is the first study to identify methylation changes in the GNAS locus,improving the diagnosis of malignant pancreatic cysts and suggesting a role in progression to PDAC.

关键词： Intraductal papillary mucinous neoplasms Pancreas cyst Methylation Biomarker GNAS locus Pancreatic neoplasm

Dietary supplementation with free methionine or methionine dipeptide mitigates intestinal oxidative stress induced by Eimeria spp. challenge in broiler chickens

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Journal of Animal Science and Biotechnology 2019年第4期10卷 1005-1021页

作者： Angélica de Souza Khatlab Ana paula Del Vesco Adhemar Rodrigues de Oliveira Neto Roberta pereira Miranda Fernandes Eliane Gasparino Animal Science Department State University of MaringáColombo Avenue5790Jardim UniversitárioMaringáParaná87020-900Brazil Animal Science Department Federal University of SergipeMarechal Rondon AvenueS/NJardim Rosa ElzeSao CristóvaoSergipe 49100-000Brazil EVONIK of Brazil Arquiteto Olavo Redig de Campos Street105Tower ASo PauloSP 04711-904Brazil Physiology Departament Federal University of SergipeMarechal Rondon AvenueS/NJardim Rosa ElzeSao CristóvaoSergipe 49100-000Brazil

Background: This study evaluated the effects of Eimeria spp. challenge and dietary supplementation with free methionine or methionine dipeptide on animal performance;expression of genes associated with the immune system, antioxidant system, and amino acid transport in the jejunum;and redox status of the jejunum of broiler ***: A randomized, 2 × 3 factorial design was used, in which Eimeria spp. challenge was the first factor(Eimeria-challenged, EC, or unchallenged, UC, broilers) and methionine supplementation was the second factor(non-supplemented, NS;free DL-methionine, DL-Met;and methionine dipeptide, DL-methionyl-DL-methionine, DLMMet). At 14 days of age, chickens were inoculated orally with sporulated oocysts of Eimeria acervulina, Eimeria praecox, Eimeria maxima, and Eimeria mitis. Birds were killed by cervical dislocation 144 h post-inoculation(PI), and the jejunum was collected for biochemical and molecular ***: EC broilers had a 13% lower feed intake(FI), 37% lower body weight gain(BWG), and 39% higher feed conversion ratio(FCR) than UC broilers. Chickens fed the DL-Met diet had higher BWG(about 12% higher) and better FCR(about 12% lower) than chickens fed the NS diet. EC chickens had lower relative weight of the bursa of Fabricius(51.8%) and higher relative weights of the spleen and whole intestine(53.6% and 26.3%, respectively) than UC chickens. Eimeria spp. challenge led to an increase in the levels of oxidative substances, such as nitrite and thiobarbituric acid reactive substances(TBARS), in the jejunum of chickens 144 h PI. Among UC chickens, those fed the DL-Met diet had higher total antioxidant capacity(TAC) and lower catalase(CAT) and superoxide dismutase(SOD) activities. EC chickens that received the NS diet had higher carbonylated protein content(CP). This result was associated with their lower TAC and catalase activity. The lower TAC in EC chickens might have been due to reduced expression of catalase(CAT) and superoxide

关键词： Antioxidant Coccidiosis Gut INFG PEPT1

-765G > C COX-2 polymorphism may be a susceptibility marker for gastric adenocarcinoma in patients with atrophy or intestinal metaplasia

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World Journal of Gastroenterology 2006年第34期12卷 5473-5478页

作者： Carina pereira Hugo Sousa paula Ferreira Maria Fragoso Luís Moreira-Dias Carlos Lopes Rui Medeiros Mário Dinis-Ribeiro Molecular Oncology Group Portuguese Institute of Oncology of Porto Portugal and ICBAS Abel Salazar Institute for the Biomedical Sciences Porto Portugal Oncology Department Portuguese Institute of Oncology of Porto Portugal Gastroenterology Department Portuguese Institute of Oncology of Porto Portugal and Cintesis / Faculty of Medicine University of Porto Porto Portugal

AIM： To investigate the relationship between the -765G 〉 C COX-2 polymorphism and the development of different gastric lesions： atrophy or intestinal metaplasia and gastric adenocarcinoma. METHODS： A cross-sectional study was performed involving 320 Portuguese individuals （210 without evidence of neoplastic disease, 73 patients with gastric adenocarcinomas and 37 with atrophy or intestinal metaplasia） using a PCR-RFLP method.RESULTS： -765C allele was overrepresented in the patients with gastric adenocarcinoma （51%） when compared either with the control group （38%） or patients with atrophy or intestinal metaplasia （27%）. Callele was found to be very common in our population （0.22）, and a multivariate logistic regression analysis revealed nearly 3-fold increased risk for the progression to gastric adenocarcinoma in patients with atrophy or intestinal metaplasia carrying the -765C allele （OR = 2.67, 95% CI = 1.03-6.93; P = 0.04）.CONCLUSION： -765C carrier status should be considered as another susceptibility marker for gastric adenocarcinoma development in patients with atrophy or intestinal metaplasia.

关键词： Gastric adenocarcinoma Atrophy Intestinal metaplasia COX-2 Polymorphism PCR-RFLP Pharmacogenomic

In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum

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Journal of Integrative Medicine 2018年第6期16卷 404-410页

作者： Bruno Jose Martins Da Silva Sandro Wilson Gomes pereira Ana paula Drummond Rodrigues Jose Luiz Martins Do Nascimento Edilene Oliveira Silva Laboratory of Structural Biology Institute of Biological SciencesFederal University of PardBelemPard 66075-110Brazil National Institute of Science and Technology in Structural Biology and Bioimaging(INCT-INBEB) Rio de Janeiro 21941-901Brazil Laboratory of Electron Microscopy Department of Health SurveillanceMinistry of HealthEvandro Chagas InstituteBelemPard 66087-082Brazil Laboratory of Molecular and Cellular Neurochemistry Institute of Biological SciencesFederal University of PardBelemPard 66075-110Brazil National Institute of Science and Technology in Neuroimmunomodulation(INCT-NIM) Rio de Janeiro 21040-360Brazil

Objective： In the present study, we evaluated the effects of the aqueous extract of Physalis angulata root （AEPa） on Leishmania infanturn proliferation, morphology, and the driving mechanism in leishmanicidal activity and modulatory action on macrophages. Methods： I., infantum promastigotes were treated with 50 and 100 μg/mL AEPa for 72 h and then antipro- mastigote assay was performed by counts in a Newbauer chamber, morphological changes were analyzed by transmission electron microscopy and the mechanism of the leishmanicidal activity was detected. In addition, macrophages were infected with L. infantum and were used to evaluate anti-amastigote activity of AEPa and effects of AEPa on cytokine secretion after 72-hour treatment. Results： Treatment with AEPa reduced the numbers ofL infantum promastigotes （50% inhibitory concen- tration （ICso） = 65.9μg/mL; selectivity index （SI）= 22.1） and amastigotes （ICso = 37.9 μg/mL; SI = 38.5） compared with the untreated control. Amphotericin B reduced 100% of the promastigote numbers after 72 h of treatment （IC50 = 0.2μg/mL）. AEPa induced several morphological changes and increased the production of reactive oxygen species and apoptotic death in promastigotes after treating for 72 h. AEPa （100 μg/mL） promoted tumor necrosis factor-α secretion in macrophages infected with L. infantum after 72 h of treatment, but did not induce an increase in this cytokine in noninfected macrophages. In addition, AEPa showed no cytotoxic effect on J774-A1 cells （50% cytotoxic concentration 〉1000μg/mL）. Conclusion： AEPa presented antileishmanial activity against the promastigotes and amastigotes of I.. infantum without macrophage cytotoxicity; these results show that natural products such as P. angulata have leishmanicidal potential and in the future may be an alternative treatment for leishmaniasis.

关键词： Leishmanicidal activity Leishmania infantum Reactive oxygen species Apoptosis Physalis angulata

Genoprotection and metabolic benefits of marine macroalgae-Insights into the concept of functional foods through direct and indirect consumption

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Food Bioscience 2022年第3期47卷 524-533页

作者： Ana Marques João Ferreira Ana Cerqueda-Pacheco Vitória pereira Helena Abreu Rui pereira Maria João Pires Fernanda Seixas paula Oliveira Isabel Gaivão Mário Pacheco Laboratory of Physiotoxicology CESAM-Centre for Environmental and Marine StudiesDepartment of BiologyUniversity of AveiroCampus Universitário de Santiago3810-193AveiroPortugal CECAV-Animal and Veterinary Research Center AL4AnimalsUniversity of Trás-os-Montes and Alto DouroQuinta de Prados5000-801Vila RealPortugal Department of Genetics and Biotechnology University of Trás-os-Montes and Alto DouroQuinta de Prados5000-801Vila RealPortugal Department of Biology and Environment University of Trás-os-Montes and Alto DouroQuinta de Prados5000-801Vila RealPortugal CITAB-Centre for the Research and Technology of Agro-Environmental and Biological Sciences Inov4AgroUniversity of Trás-os-Montes and Alto DouroQuinta de Prados5000-801Vila RealPortugal Department of Veterinary Sciences University of Trás-os-Montes and Alto DouroQuinta de Prados5000-801Vila RealPortugal ALGAplus Lda.Travessa Alexandre da Conceição s/n3830-196ÍlhavoPortugal

The consumption of marine macroalgae has been linked with health benefits,albeit some actions,including genoprotection,remain ***,we evaluated the genoprotective potential of a mixture of Ulva rigida,Fucus vesiculosus and Gracilaria gracilis,through direct consumption or via indirect intake using fish(Sparus aurata)as a vector of algal ***(Mus musculus)were subjected to a 1-month dietary supplementation with 5%of macroalgae mix or 10%of fillet of fish previously fed with an algae-supplemented aquafeed(for comparison purposes,10%supplementation with fillet of fish standardly fed was also considered).Thereafter,mice were challenged by the genotoxicant methyl methanesulfonate(MMS),and the genetic damage was evaluated through the micronuclei ***,a wider evaluation was carried out encompassing the assessment of energy metabolism,haematological and histological parameters,as well as growth *** unequivocally protected against MMS-induced *** activity profile of hepatic lactate and isocitrate dehydrogenases promoted by the supplemented diets reflected an improved energy *** properties were not transferred via fish fed with the algae-supplemented aquafeed,though the transference of phytocomponents with beneficial properties(*** balance improvement)should not be ***,these findings highlighted the genoprotection afforded by marine macroalgae,likely promoted by desmutagenic factors,reinforcing their definition as functional *** possibility of using fish as an indirect source of algae-borne phytocomponents proved to be plausible and worthy of further investigations.

关键词： Antigenotoxicity Desmutagens Fish consumption Functional food Marine macroalgae consumption Metabolic benefits

Engineering Improved Photosynthesis in the Era of Synthetic Biology

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Plant Communications 2020年第2期1卷 1-17页

作者： Willian Batista-Silva paula da Fonseca-pereira Auxiliadora Oliveira Martins Agustin Zsogon Adriano Nunes-Nesi Wagner L.Araujo Departamento de Biologia Vegetal Universidade Federal de Vic¸osaVic¸osaMinas Gerais 36570-900Brazil

Much attention has been given to the enhancement of photosynthesis as a strategy for the optimization of crop *** traditional plant breeding is most likely reaching a plateau,there is a timely need to accelerate improvements in photosynthetic efficiency by means of novel tools and biotechnological *** emerging field of synthetic biology offers the potential for building completely novel pathways in predictable directions and,thus,addresses the global requirements for higher yields expected to occur in the 21st ***,we discuss recent advances and current challenges of engineering improved photosynthesis in the era of synthetic biology toward optimized utilization of solar energy and carbon sources to optimize the production of food,fiber,and fuel.

关键词： photosynthesis synthetic biology genetic engineering yield improvement targeted manipulation

Ecophysiology and multivariate analysis for production of Tachigali vulgaris in Brazil:Influence of rainfall seasonality and fertilization

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Journal of Forestry Research 2023年第5期34卷 1289-1305页

作者： Pedro Henrique Oliveira Simoes Candido Ferreira de Oliveira Neto Manoel Tavares de paula Dênmora Gomes de Araújo Rodrigo Silva do Vale Joao Olegário pereira de Carvalho Universidade Federal de Mato Grosso Campus Universitario de SinopSinop 78550728Mato GrossoBrazil Universidade Federal Rural da Amazonia Campus BelemBelem 66077830ParaBrazil Universidade do Estado do Para Centro de Ciencias Naturais e TecnologiaBelem 66095015ParaBrazil Universidade Federal Rural da Amazonia Campus Capitao-PocoCapitao Poco 68650000ParaBrazil

Studies on fertilization management of species native to the Amazon for energy plantations contribute to the diversity of species use and reduce biological risk due to the excessive use of clones or hybrids of *** study evaluates the effect of precipitation seasonality and phosphorus and potassium fertilization on gas exchange in a Tachigali vulgaris *** levels of P(zero,65.2,130.4 kg ha^(-1))and three of K(zero,100.0,200.0 kg ha^(-1))were applied in a 3×3 factorial randomized block *** exchange measurements were conducted in April and November *** low rainfall,high irradiance period,photo synthetic rates were up to four times higher than in the high rainfall period,reaching 20.3μmol m^(-2)s^(-1)in the treatment with 130.4 g kg^(-1)of P and 100.0 g kg^(-1)of *** analysis and principal component analysis reduced the initial eight gas exchange variables to two and three principal components in periods of high and low rainfall,*** multivariate method used in this study readily identified variations in the variables as a function of rainfall,with high reliability in explaining the data set.

关键词： Photosynthesis rate Stomatal conductance Principal component analysis Factor analysis Tachigali vulgaris