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Use of Agaricus bisporus mushroom in beef burgers:antioxidant,flavor enhancer and fat replacing potential

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食物生产加工与营养(英文) 2019年第1期1卷 64-78页

作者： Iliani Patinho Erick Saldaña Miriam Mabel Selani Adriano Costa de Camargo Thais Cardoso Merlo Beatriz Schmidt Menegali Anna paula de souza Silva Carmen J.Contreras-Castillo departamento de Agroindústria Alimentos e Nutrição(LAN)Escola Superior de Agricultura“Luiz de Queiroz”(ESALQ)Universidade de São Paulo(USP)Avenida Pádua Dias11PiracicabaSP 13418-900Brazil Centro de Ciências da Natureza Campus Lagoa do SinoUniversidade Federal de São CarlosRod.Lauri Simões de BarrosKm 12BuriSPBrazil departamento de Ciencias Vegetales Facultad de Agronomía e Ingeniería ForestalPontificia Universidad Católica de ChileCasilla306-22 SantiagoChile Facultad de Ingeniería Agroindustrial Universidad Nacional de Moquegua(UNAM)MoqueguaPeru

This study aimed to evaluate the potential use of Agaricus bisporus mushroom as a natural antioxidant,fat/salt substitute and flavor enhancer in beef *** treatments were considered using a fractional factorial design(salt[0.5,1.25 and 2.0%],fat[10,15 and 20%]and mushroom content[0,15 and 30%]).Treatments were characterized by instrumental and sensory *** statistical design indicated that fat content had little influence on the *** principal component analysis showed that the incorporation of mushrooms and salt modified the texture,moisture and water *** stability decreased as the salt content increased,at a low-fat ***,the sensory profile was affected by the mushroom and salt contents,with the fat content exerting the least ***,mushroom incorporation in beef burger may be a feasible strategy to reduce the fat content of beef burgers.

关键词： Beef burger Agaricus bisporus Lipid oxidation Texture profile analysis descriptive analysis

Integrating species and successional classes for wood production in a mixed forest restoration system in a neotropical region

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Journal of Forestry Research 2021年第6期32卷 2313-2321页

作者： Ananias Francisco Dias Junior Ana Lucia Martins Sodero Pincelli Ana paula Camara da Silva Alison Moreira da Silva Natalia Dias de souza Mario Tommasiello Filho Jose Otavio Brito Wood and Forestry Science department Federal University of EspIrito Santo(UFES)Av.Governador Lindemberg316JerOnimo Monteiro CityES 29550-000Brazil São Paulo State School of Technology(FATEC/SP) Av.DiacOno Jair de Oliveira651PiracicabaSP 13414-141Brazil Forest Products department Federal Rural University of Rio de Janeiro(UFRRJ)BR 465km 07SeropEdicaRJ 29890-000Brazil University of SAo Paulo “Luiz de Queiroz”College of Agriculture(USP/ESALQ)Av.PAdua Dias11PiracicabaSP 13418-900Brazil

As forests in neotropical regions,particularly in developing countries,are devastated,interventions to restore biodiversity and its ecological functions are *** producers have thus been encouraged to grow trees for wood production as an economic *** objective of this study was to quantify the increment of wood density of four forest species from different successional classes of a mixed system of restoration in a neotropical forest in *** discs were sampled at breast height and analyzed radially by X-ray densitometry to obtain apparent density and basic *** trees each of a species from the pioneer,early and late secondary successional stages and of the dominant species in the climax *** radial profiles indicated increasing density from the pith to bark of trunks,except for some variations due to wood defects and *** density was 0.576 g cm^(-3),0.655 g cm^(-3),0.706 g cm^(-3)and 0.775 g cm^(-3),respectively,for Peltophorum dubium,Schinus terebinthifolius,Cariniana estrellensis and Hymenaea *** profiles indicated higher amplitudes in the apparent densities for slow-growing species.X-ray densitometry generated parameters such as minimum,average and maximum densities,and radial density *** parameters are important for understanding the ecological functional role of successional classes of the Atlantic Forest from the Neotropical region.

关键词： X-ray densitometry Timber species of neotropical forest Successional classes of forest species Maintenance of forest restoration

Resistance mechanism to cisplatin in NCI-H460 non-small cell lung cancer cell line:investigating apoptosis,autophagy,and cytogenetic damage

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Cancer Drug Resistance 2018年第1期1卷 72-81页

作者： Érica Ballestreri Daniel Simon Ana paula de souza Camila Schultz Grott Débora Dreher Nabinger Rafael Rodrigues Dihl Ivana Grivicich Laboratory of Cancer Biology Graduate Program in Cellular and Molecular Biology Applied to HealthLutheran University of BrazilCanoasRS 92425-900Brazil Laboratory of Human Molecular Genetics Graduate Program in Cellular and Molecular Biology Applied to HealthLutheran University of BrazilCanoasRS 92425-900Brazil Laboratory of Genetic Toxicity Graduate Program in Cellular and Molecular Biology Applied to HealthLutheran University of BrazilCanoasRS 92425-900Brazil

Aim:To investigate the effects of cisplatin on the human non-small cell lung carcinoma(NCI-H460)cell line regarding cytotoxicity,genotoxicity,and expression of genes associated with apoptosis(BIRC5)and autophagy(BECN1).Methods:Cell cultures were treated with cisplatin concentrations(0.16-33.3μmol/L)for 48 *** and acute and chronic cytotoxicities were assessed using the MTT,clonogenic,and cytokinesis-block micronucleus *** expression of BIRC5 and BECN1 was evaluated by reverse transcription-polymerase chain ***:Cisplatin IC50(0.33μmol/L)increased micronucleus frequency 2.50 *** was also cytotoxic in the 0.6-33.3μmol/L range,with reduced expression of the BIRC5 gene,suggesting induction of *** reducing the expression of the BIRC5 gene,33.3μmol/L cisplatin increased the expression of the BECN1 gene,suggesting that autophagy can be related to cisplatin ***:Cisplatin inhibited NCI-H460 growth,and cisplatin IC50 induced genotoxic *** higher cisplatin concentrations are used,the expression of genes associated with apoptosis and autophagy was *** results points to a further investigation of the role of autophagy in cisplatin resistance.

关键词： BECN1 BIRC5 cisplatin cytotoxicity lung cancer

Analyzing Brazilian Driver’s License Authenticity by Easy Ambient Sonic-Spray Ionization Mass Spectrometry

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American Journal of Analytical Chemistry 2016年第4期7卷 342-350页

作者： deleon Nascimento Correa Eduardo Morgado Schmidt Marcos Fernando Franco Jorge Jardim Zacca Werickson Fortunato de Carvalho Rocha Antony de paula barbosa Rodrigo Borges Wanderley de souza Marcos Nogueira Eberlin ThoMSon Mass Spectrometry Laboratory Department of Analytical Chemistry Institute of Chemistry University of Campinas-UNICAMP Campinas Brazil Technical-Scientific Police Superintendency from Sao Paulo State Criminalistic Institute Sao Paulo Brazil Analítica Sao Paulo Brazil Brazilian Federal Police Scientific and Technical Department Brasília Brazil National Institute of Metrology Quality and Technology-INMETRO Duque de Caxias Brazil

Fast and unequivocal methods of questioned document analysis are essential in forensic science. Here, a desorption/ionization technique, EASI-MS, was assessed for its ability to investigate questioned driver’s licenses (DL). Two suspects DL, displaying the same personal data in the proper fields (name and ID numbers), but with different individual photos, showing similar impressions on microscopic analysis, and authentic standards documents specimens were used as test cases. Profiles from authentic DL surface were dominated by a set of few minor ions, mainly from the plasticizers bis(2-ethylhexyl)phthalate and dibutylphthalate. The seized suspect counterfeit DL on points from personal data and photo were, however, dominated by abundant diagnostic ions of m/z 463, 507, 551, 595, 639, 683, which confirmed counterfeiting. Surfynol® and Nonoxynol-9®, which are common constituents of inkjet printing, were detected in the counterfeiting areas by high-accuracy EASI(+)-FT-ICR MS. The EASI-MS technique is shown therefore to offer an attractive tool for forensic investigation of questioned documents.

关键词： Forensic Sciences Questioned Documents Ambient Mass Spectrometry Easy Ambient Sonic-Spray Ionization Mass Spectrometry EASI-MS

Prevalence of congenital cytomegalovirus infection in preterm,small for gestational age and low birth weight newborns:characteristics and cytokines profile

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World Journal of Pediatrics 2022年第7期18卷 498-504页

作者： Janaina Fortes Lino Llian Martins Oliveira Diniz debora Marques de Miranda Daniela Valadao Freitas Rosa Nathalia Gualberto souza e Silva Eduardo de souza Nicolau Larissa Goncalves Rezende Lais Silva Carvalho Marianna Fischer de paula Lopes Luisa Petri Correa Gabriela Mafra de Oliveira Flavia Miranda da Silva Alves Lorena Batista Pascoal Erika Lima Dolabella Teixeira da Costal Leni Marcia Anchieta Roberta Maia de Castro Romaneli Odete Valadares Maternity Hospital Belo HorizonteBrazil Pediatrics department.Faculy of Medice of Federal University of Minas Gerais Belo Horizonte.Brazil Molecular Laboratory of the INCT-MM.Faculy of Medicine.Federal University of Minas Gerais-UFMG.Belo Horizonte Brazil Faculty of Medicine.Federal University of Minas Gerais-UFMG Belo Horizonte.Brazil Risoleta Tolentino.Neves University Hospital's Maternity Belo HorizonteBrazil Sofia Feldman Maternity Hospital Belo HorizonteBrazil Hospital das Clinicas Maternity-Federal University of Minas Gerais.Belo Horizonte Brazil Pediatrics department of Federal University of Minas Gerais School Medicine.Minas Gerais.Av Professor Alfredo Balena 190.Belo Horizonte 30130-100Brazil

Cytomegalovirus is the most frequent agent of congenital viral infections,affecting approximately 0.2%-2.4%of live births in different countries[1-7].Universal screening for congenital cytomegalovirus infection in newborns is not a standard practice considering cost-benefit issues and viabil-ity of diagnostic ***,most newborns are asympto-matic and there is no effective treatment for this population[2-5].

关键词： weight birth characteristics

Employment of Waste from the Textile Industry for the Production of Nanocomposites Aiming at the Generation of Thermal Shrinkable Films and the Non-Formation of Microplastics

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Materials Sciences and Applications 2023年第8期14卷 426-438页

作者： Dian souza Garcia Bruno de paula Amantes Maria Inês Bruno Tavares Instituto de Macromoléculas Professora Eloisa Mano Universidade Federal do Rio de Janeiro Centro de Tecnologia Rio de Janeiro Brasil

With the increase of incorrectly discarded materials in the environment, including clothes, the need for recycling and reusing them becomes one of the alternatives to work around this problem, following the law of the 3 Rs. However, these polymeric materials which are deposited in the marine environment and land generate microplastics, due to their degradation/fragmentation due to the action of the weather, in these regions, including being carried by the air and transported from various regions. With the aim of reducing the impact of this inappropriate disposal and obtaining a material that does not generate microplastics, this study used waste from the textile industry, which would be unusable, and a biodegradable polymer. The tissue residues were submitted to acid hydrolysis with sulfuric acid, in order to weaken the interactions between the cellulose fibrils and promote a better dispersion of the latter when processed with the polymer, during the extrusion process, generating biodegradable polymeric nanocomposites. The results showed that the samples submitted to acid hydrolysis presented a higher degree of crystallinity and a greater number of interactions and lower mobility of hydrogen atoms. In addition, the samples treated for a longer time showed a small release of fibrils, suggesting that this treatment can help in the production of nanocomposites during their extrusion.

关键词： Microplastic Nanotechnology Cellulose Textile Industry Biodegradable Polymer

Pediatric functional constipation treatment with Bifi dobacterium-containing yogurt:A crossover,double-blind,controlled trial

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World Journal of Gastroenterology 2011年第34期17卷 3916-3921页

作者： paula VP Guerra Luiza N Lima Tassia C souza Vanessa Mazochi Francisco J Penna Andreia M Silva Jacques R Nicoli Elizabet V Guimares departamento de Pediatria Faculdade de Medicina Universidade Federal de Minas Gerais30130-100 Belo Horizonte MG Brazil departamento de Micro-biologia Instituto de Ciências Biológicas Universidade Federalde Minas Gerais32270-901 Belo Horizonte MG Brazil Andreia M Silva Universidade Federal deSo Joo del Rei Campus de Sete Lagoas35701-970 So Joodel Rey MG Brazil

AIM: To evaluate the treatment of pediatric functional chronic intestinal constipation (FCIC) with a probiotic goat yogurt. METHODS: A crossover double-blind formula-controlled trial was carried out on 59 students (age range: 5-15 years) of a public school in Belo Horizonte, MG, Brazil, presenting a FCIC diagnostic, according to Roma Ⅲ criteria. The students were randomized in two groups to receive a goat yogurt supplemented with 109 colony forming unit/mL Bifidobacterium longum (***) (probiotic) daily or only the yogurt for a period of 5 wk (formula). Afterwards, the groups were intercrossed for another 5 wk. defecation frequency, stool consistency and abdominal and defecation pain were ***: Both treatment groups demonstrated improvement in defecation frequency compared to baseline. However, the group treated with probiotic showed most signif icant improvement in the f irst phase of the study. An inversion was observed after crossing over, resulting in a reduction in stool frequency when this group was treated by formula. Probiotic and formula improved stool consistency in the f irst phase of treatment, but the improvement obtained with probiotic was significantly higher (P = 0.03). In the second phase of treatment, the group initially treated with probiotic showed worseningstool consistency when using formula. However, the difference was not signif icant. A signif icant improvement in abdominal pain and defecation pain was observed with both probiotic and formula in the first phase of treatment, but again the improvement was more signif icant for the group treated with B. longum during phase I (P defecation frequency (P = 0.012), defecation pain (P = 0.046) and abdominal pain (P = 0.015).

关键词： Functional chronic constipation Probiotic Bifi dobacterium longum Yogurt Adolescents Children

Late diagnosis and TCD8 immune response profile of cutaneous tuberculosis: A case report

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Open Journal of Immunology 2012年第3期2卷 125-131页

作者： Bruna Daniella de souza Silva Sueli Lemes de ávila Alves André Kipnis Ana paula Junqueira-Kipnis departamento de Microbiologia Imunologia Patologia e Parasitologia Instituto de Patologia Tropical e Saúde Pública Universidade Federal de Goiás Goiania/GO Brazil Laboratório Central de Saúde Pública Dr. Giovanni Cysneiros LACEN/GO Goiania/GO Brazil

Introduction: Cutaneous tuberculosis (CTB) is a rare form of extra-pulmonary tuberculosis that, when associated with late diagnosis, worsen the quality of life of the sick individuals. This report presents a case of late diagnosis of CTB. Unusual clinical manifestations retarded the correct tuberculosis diagnosis for more than a year. The immune response elicited by this type of tuberculosis as well as the factors that might contribute to the delay in diagnosis was evaluated and discussed. Methodology: Clinical evaluation and flow cytometric analyses of PBMC were realized for a case of CTB and a healthy individual as a control. Results: M. tuberculosis specific TCD8+ cell response was analyzed by flow cytometry and revealed positive cells for IL-17, IL-10, TGF-β and IDO. The CTB patient presented higher percentage of those cells when compared to a healthy donor. However, TCD8 cells positive for the important protective cytokine, IFN-γ was decreased in the CTB patient. Conclusion. The assessment of the M. tuberculosis specific TCD8+ immune response showed a regulatory/modulatory phenotype with a reduced IFN-γ response when compared to the healthy control that could have contributed to the CTB infection.

关键词： Cutaneous tuberculosis Late Diagnosis CD8+ T Cells

Where Are the Bromeliads? A Study on Different Trails in the Ecological Reserve of Guapiaçu, RJ

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Agricultural Sciences 2023年第2期14卷 196-201页

作者： Johnatan Jair de paula Marchiori Vinicius de souza Oliveira Gilcéa Teixeira Fontana Boone Luan Luciano dos Santos Pereira Natália Cristina barbosa Moura Ana Beatriz Mamedes Piffer Anderson Mathias Holtz Lusiane de Sousa Ferreira Raquel Cetto Sampaio Federal Rural University of Rio de Janeiro Seropédica Brazil Center for Agricultural Sciences and Engineering Federal University of Espírito Santo Alegre Brazil Campus Itapina Federal Institute of Espírito Santo Colatina Brazil State University Paulista “Júlio de Mesquita Filho” Botucatu Brazil

Bromeliaceae are important in ecological restoration, showing easy adaptation to different environments, and constantly interacting with each other through ecological relationships, whose effects may or may not benefit the species involved. The hypothesis tested whether the population density of Hohenbergia augusta is higher in areas at a more advanced successional stage (brown trail), since the species is typical of more structured forest environments, occurring especially in the lower strata of the forest. Six plots of 10 meters (6 × 10 m) were installed, parallel to the brown and yellow trails, and with a distance of 5 m from the edge. Plots were allocated alternately between the two sides of the trail, 10 meters apart from each other when on the same side. In each plot, all H. augusta (Vell.) E. Morren bromeliads were sampled, without a minimum inclusion criterion, provided they were epiphytic phorophytes. The logarithm of the value was used for data normalization and later, the data were tested in a student’s t-test. The study pointed out that the highest population density of H. augusta occurred in the brown trail because the area is in a more advanced successional stage.

关键词： Bromeliads Atlantic Forest Ecological Restoration