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中国植物病理学会2019年学术年会

作者： Yin Qiaoxiu Ren Yafeng Li Dongxue Wang Xue Dharmasena dissanayake-saman-pradeep Jiang Shilong Wu Xian Song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Agriculture Guizhou University

Tea leaf spot was an important disease of tea plant(Camellia sinensis) in Yuqing county,Guizhou *** disease mainly damaged the young leaves and shoots tea,and then led to a huge loss of tea *** symptoms were light brown spots,which was gradually increased in size and developed into brown,scattered,elliptical or irregular *** lesions later became greyish at the central,and then were surrounded by an apparent bright yellow *** adjacent lesions have been merged to the larger lesion,which was covered the margin of the *** this study,we isolated and identified some fungi from tea leaves samples with the symptoms of leaf spot,and then conducted the pathogenicity test using Koch’s *** colony was white to pale yellow and produced masses of flocculent aerial mycelium,with an average growth rate of 12.9±0.062 mm/d on PDA after 7 days of post-inoculation in the *** colony displayed pale yellow on reverse *** 22 days of post-inoculation,the colonies represented white to rosy *** produced on PDA were usually globose to subglobose,scattered or gregarious,olivaceous to olivaceous *** unicellular and hyaline conidia represent ellipsoidal or obovate,(4.09-9.04) μm×(1.77-4.06)μm,which usually became rounded in both ends.28 S nrDNA(LSU),internal transcribed spacer(ITS),β-tubulin 2(β-TUB2)was amplified with the primer pair LR0 R/LR7,V9 G/ITS4,Btub2 Fd/Btub4 Rd for the isolates,and then the sequences were deposited in *** parsimony phylogenetic analysis based on concatenated sequences of combined ITS(1-493),LSU(494-1897),β-TUB2(1898-2254) indicated that the isolate GZYQ-5-2-b was identical to reference strains Didymella bellidis PD 94/886,and the clade was supported by 100% bootstrap *** fulfill Koch’s postulates,the tea leaves of two tea varieties(Moss tea and Fuding Dabai cha) were inoculated with mycelial plugs on PDA or the spore suspension(1×10 spores per mL) using the methods of unwound and wou

关键词： Didymella bellidis identification pathogenicity test leaf spot

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Metabolome changes of tea leaf spot pathogen Phoma segeticola *** cultured on PDA with tea liquor

Metabolome changes of tea leaf spot pathogen Phoma segeticol...

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中国植物病理学会2019年学术年会

作者： Ren Yafeng Li Dongxue Wang Xue Yin Qiaoxiu Dharmasena dissanayake-saman-pradeep Jiang Shilong Wu Xian Song Baoan Chen Zhuo Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of EducationGuizhou University College of Agriculture Guizhou University

The fungal pathogen Phoma segeticola *** causes leaf spot on tea(Camellia sinensis) in Guizhou Province,*** study the mechanism of metabolomics during the stage of the pathogen infection,we design the experiment scheme of the pathogen culturing on PDA or PDA with tea liquor(Tea variety:Fudingdabaicha).The samples were detected using Nano-LC-MS-MS(TripleTOF 5600) under the mode of positive ion and negative ion,and then the data of the metabolites was further identified and quantified using the software XCMS and *** MS and MS-MS of the metabolites were annotated using the software metaX and in-house fragment spectrum *** were12 624 features in the positive mode,and 524 could be identified by ***,1 1008 features were detected in the negative mode,and 323 could be identified by *** addition,10850 high quality features were used to quantity following condition:ratio≥2 or ratio≤1/2;q value≤0.05;VIP≥1.122 2 and 1 048 were up-or down-regulated in the positive mode,*** the negative mode,9917 high quality features were used to quantity,and 1 192 and 1 078 features were up-or down-regulated,*** to the partial least-squares discrimination analysis,R value was 1.00,and Q2 value was 0.98 in the positive *** R value was 1.00,and Q2 value was 0.99 in the negative *** different metabolites were identified in 117 pathways,such as Biosynthesis of secondary metabolites(204),Biosynthesis of antibiotics(128),Microbial metabolism in diverse environments(95) and Arachidonic acid metabolism(45) et *** data would provide important reference for the pathogenesis of tea leaf spot.

关键词： Phoma segeticola var.camelliae Camellia sinensis metabolome

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Comparative transcriptome profiling of mRNA and lncRNA related to tea leaves infected by Phoma segeticola ***

Comparative transcriptome profiling of mRNA and lncRNA relat...

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中国植物病理学会2019年学术年会

作者： Li Dongxue Wang Xue Yin Qiaoxiu Ren Yafeng Bao Xingtao Dharmasena dissanayake-saman-pradeep Wu Xian Song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University

Non-coding RNAs(ncRNAs) such as long non-coding IncRNA have been reported to play important roles in the regulation of many biological *** rapid development of omics sequencing technology has promoted the identification of thousands of lncRNAs in plant species,but the role of IncRNAs in plant-pathogen interactions remains largely *** identified significant differentially expressed genes(DEGs) andlncRNAs(DELs),and then construct lncRNA-mRNA networks using comparative transcriptome analysis of the leaves of tea plant(Camellia sinensis) infected and uninfected by Phoma segeticola ***,coupled with bioinformatics.A total of 1615 DEGs and 561 DELs(including 146 up-regulated IncRNAs and 415 down-regulated lncRNAs,P<0.05 and I log≥1) were identified between tea leaves infected and uninfected by *** *** network of co-localization was constructed for the analysis of the interaction of DEGs and *** DEGs may be cis-regulated by DELs were enriched in 10 terms containing with NADP binding and sucrose alpha-glucosidase activity,et al(P<0.05).By the enrichment analysis of KEGG,4 DELs were most enriched in the pathway of biosynthesis of amino acids(P<0.05).Integrated the analysis of the data of DEGs and DELs,6 pairs of significant co-expression pairs were screened,with 5 positively correlated pairs(COR≥0.7) and 1 negatively correlated pair(COR≤-0.7).As the differentially expressed trends of lncRNAs,IncRNA118096(MSTRG.70289.1) and lncRNA20211(MSTRG.12101.1) may play crucial roles in plant defense *** summary,the present study extends the IncRNA database of the tea leaves and these IncRNAs may provide reference on the study of plant-pathogen interactions in the future.

关键词： tea leaves Phoma segeticola var.camelliae lncRNA co-expressing

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Draft genome resource of the tea leaf spot pathogen Didymella segeticola

Draft genome resource of the tea leaf spot pathogen Didymell...

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中国植物病理学会2019年学术年会

作者： Li Dongxue Ren Yafeng Wang Xue Yin Qiaoxiu Bao Xingtao Dharmasena dissanayake-saman-pradeep Wu Xian Song Baoan Chen Zhuo State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University College of Agriculture Guizhou University

The fungal pathogen Didymella segeticola（Basionym:Phoma segeticola） causes leaf spot on tea（Camellia sinensis）,which leads to a loss in tea leaf production in Guizhou Province,*** isolate GZSQ-4 was sequenced on Illumina Hi-Seq platform,generating 53,530,422 raw *** Illumina reads were trimmed,corrected and filtered.A total of 51,270,673 clean reads passed the quality control criteria and were used in subsequent analyses of *** isolate ***,a Pacific Biosciences（PacBio） RS library was prepared using G-tubes and sequenced using single-molecule real-time cells,generating PacBio *** reads were further filtered,generating PacBio subreads,with 1 489 032 total *** largest length was 63 621 bp,and the N50,N90 and average lengths were 10 453 bp,5 327 bp and 7 939 bp,*** genome size was～36.7 Mbp based on read sequence data being processed with the condition of 21-mers using the K-mer statistical *** whole-genome assembly was～33.4 Mbp with a scaffold N50 value of-2.3 *** total,10 893 genes were predicted using the Non-redundant,Gene Ontology,Clusters of Orthologous Groups,Kyoto Encyclopedia of Genes and Genomes,and SWISS-PROT *** genome sequence has been deposited in the NCBI Sequencing Read Archive database and can be accessed with the gene accession SDAV00000000（BioProject:PRJNA516041;BioSample:SAMN10768991;SRA accession:SRR8492863）.The whole-genome sequence of *** will provide a resource for future research on host-pathogen interactions,determination of trait-specific genes,pathogen evolution and plant-host adaptation mechanisms.

关键词： whole genome sequences fungal pathogen Didymella segeticola leaf spot

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Investigating the antifungal activity and mechanism of a microbial pesticide Ningnanmycin against Phoma segeticola ***

Investigating the antifungal activity and mechanism of a mic...

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中国植物病理学会2019年学术年会

Phoma segeticola *** was isolated from tea leaf spot disease in tea garden,Shiqian county,Guizhou *** leaf spot disease affected seriously the quality and yield of *** present,there are no effective measures of prevention and ***(NNM) is a new cytosine nucleoside peptide antibiotic with high efficiency and low *** method of mycelial growth rate was used to determine the antifungal activity of NNM against *** *** strain GZSQ-4,with EC of 1 287.54 U/*** order to further study the mechanism of NNM on GZSQ-4,the mycelium treated with NNM at the dosage of EC for 1 h and 14 h was observed by optical microscope,scanning electron microscopy and transmission electron microscopy,*** was found that the mycelium swelled,the mycelial cytoplasmic density decreased,the granular content appeared and the number of mitochondria granules *** GZSQ-4 being treated by NNM at the dosage of EC for 1 h,RNA-seq showed that 1 363 differentially expressed genes(DEGs) were identified with 743 DEGs upregulated and 620 DEGs *** GO enrichment analysis,the DEGs were most enriched in the structural constituent of ribosome of molecular function,ribosome of cellular component and the oxidation-reduction process of biological process including 56,38 and 116 DEGs,respectively(P<0.05).By KEGG enrichment analysis,the DEGs were most enriched in the pathway of ribosome including 58 *** selected randomly DEGs from treatment group of NNM for 1 h were verified by *** results showed that the expression level of DEGs was consistent with the result of *** results indicated that NNM may affect the processes of translation of *** study will provide useful information for management of tea leaf spot caused by *** *** and increase our understanding of action mechanism of NNM against the pathogen.

关键词： tea disease Phoma segeticola var.camellia Ningnanmycin antifungal activity action mechanism

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Transition from early intervention program to primary school in children with autism spectrum disorder

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World Journal of Clinical Pediatrics 2017年第4期6卷 169-175页

作者： Valsamma Eapen Rachel Grove Elizabeth Aylward Annette V Joosten Scott I Miller Gerdamari Van Der Watt Kathryn Fordyce Cheryl dissanayake Jacqueline Maya Madonna Tucker Antonia DeBlasio Academic Unit of Child Psychiatry South West Sydney (AUCS)ICAMHSMental Health CentreL1Liverpool Hospital School of Psychiatry Uni-versity of New South Wales Cooperative Research Centre for Living with Autism (Autism CRC) KU Marcia Burgess Autism Specific Early Learning and Care Centre School of Occupational Therapy and Social Work Curtin University Western Australia Autism Specific Early Learning and Care Centre Autism Association of Western Australia St Giles Society North West Tasmania Autism Specific Early Learning and Care Centre La Trobe University AEIOU Foundation Anglicare SA Daphne St Autism Specific Early Learning and Care Centre

AIM To evaluate the characteristics that are associated with successful transition to school outcomes in preschool aged children with *** Twenty-one participants transitioning from an early intervention program were assessed at two time points; at the end of their preschool placement and approximately 5 mo later following their transition to school. Child characteristics were assessed using the Mullen Scales of Early Learning, Vineland Adaptive Behaviour Scales, Social Communication Questionnaire and the Repetitive Behaviour Scale. Transition outcomes were assessed using Teacher Rating Scale of School Adjustment and the Social Skills Improvement System Rating Scales to provide an understanding of each child's school adjustment. The relationship between child characteristics and school outcomes was evaluated. RESULTS Cognitive ability and adaptive behaviour were shown to be associated with successful transition to school outcomes including participation in the classroom and being comfortable with the classroom teacher. These factors were also associated with social skills in the classroom including assertiveness and engagement. CONCLUSION Supporting children on the spectrum in the domains of adaptive behaviour and cognitive ability, including language skills, is important for a successful transition to school. Providing the appropriate support within structured transition programs will assist children on the spectrum with this important transition, allowing them to maximise their learning and behavioural potential.

关键词： Autism spectrum disorder School transition Primary school Outcomes

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Identifying and characterizing the circular RNAs of tea leaves inoculated with Phoma segeticola ***

Identifying and characterizing the circular RNAs of tea leav...

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中国植物病理学会2019年学术年会

As a novel class of non-coding RNAs,circular RNAs(circRNAs) have been reported to play a role in various biological ***,a little attention has been focused on plant circRNAs in the study of plant-pathogen *** study aims to explore the expression profile and functional role of circRNAs in tea leaf infected by Phoma segeticola *** used comparative transcriptome analysis of the leaves of tea plant(Camellia sinensis) to identify significant differentially expressed circRNAs(DECs) from leaves infected or un-infected by the *** candidates contained more than 98% circRNA arising from the exons of genes(mismatch≤2,back-spliced junctions reads≥1 and genomic distances to back-splicing sites were less than 100 kb).We identified349 DECs including 231 up-regulated DECs and 118 down-regulated DECs(P<0.05,|log|≥1).Gene Ontology(GO) analysis indicated that DECs were most enriched in catalytic activity of molecular functions including 11 circRNAs,intracellular membrane-bounded organelle of cellular component including 6 circRNAs and response to stress of biological process including 4 *** enrichment analysis indicated that the most enriched DECs are the pathway of purine metabolism including12 circRNAs,followed by fatty acid metabolism including 10 circRNAs(P<0.05).Based on the data of circRNAs in our study,we would provide novel insights to reveal the molecular mechanisms of plantpathogen interactions.

关键词： circRNAs Phoma segeticola var.camelliae tea leaves plant-pathogen interactions

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氟——斯里兰卡前寒武纪矿化带矿化-水文地球化学指示剂

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地球与环境 1988年第3期 39-44+31页

作者： C.B.dissanayake ,S.V.R.Weerasooriya ,曹仁琦湖南衡阳师范专科学校

在近年来的论文中,Munasinghe和Dis-sanayake(1979,1980a、b、c,1982)对斯里兰卡的地质演化提出了一个新的板块构造模式。所提模式的重要特征,是矿化带局限于两个“小板块”的边界,即现在的地质缝合线中(dissanayake和Munasinghe,1984)... 详细信息

在近年来的论文中,Munasinghe和Dis-sanayake(1979,1980a、b、c,1982)对斯里兰卡的地质演化提出了一个新的板块构造模式。所提模式的重要特征,是矿化带局限于两个“小板块”的边界,即现在的地质缝合线中(dissanayake和Munasinghe,1984)。将板块构造应用于前寒武纪还颇多疑问。

关键词：矿化带高地地质演化东维成矿带紫苏花岗岩前寒武纪前古生代杂岩斯里兰卡南亚

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斯里兰卡沉积宝石矿床中的稀土元素丰度(一)

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中国宝玉石 1986年第5期 14-17页

作者： M.S.Rupasinghe C.B.dissanayake 石其光江西地质科学研究所

这份研究报告是关于斯里兰卡含宝石沉积矿床中 REE(稀土元素)的第一份资料。在这些砂矿床中的 REE 富集程度高,尤其是轻稀土元素。标准陨石的 REE 图表表明,沉积宝石矿床中的 REE 与斯里兰卡紫苏花岗岩和世界其它地区(如瑞典西南的紫苏... 详细信息

这份研究报告是关于斯里兰卡含宝石沉积矿床中 REE(稀土元素)的第一份资料。在这些砂矿床中的 REE 富集程度高,尤其是轻稀土元素。标准陨石的 REE 图表表明,沉积宝石矿床中的 REE 与斯里兰卡紫苏花岗岩和世界其它地区(如瑞典西南的紫苏花岗质岩石)的 REE 具有显著的相似性。以富集轻稀土元素和不同的负 Eu 异常为特征模式。从宝石矿床的一般地质和 REE 资料,发现在斯里兰卡高原群的所有矿床内 REE 原生体普遍和岩浆质紫苏花岗岩共生。元素对 Er—Hf、Er—Cc、Zr—Ti 与其它各元素显示良好相关,表明沉积环境内几乎没有分馏作用。

关键词：稀土元素宝石矿床 REE 麻粒岩相紫苏花岗岩黑云母片麻岩矿物学斯里兰卡元素丰度

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Diversity of Endophytic Diaporthe associated with Citrus grandis cv“Tomentosa”in China

Diversity of Endophytic Diaporthe associated with Citrus gra...

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中国植物病理学会2021年学术年会

作者：董章勇 MANAWASINGHE Ishara Sandeepani HUANG Yinghua SHU Yongxin PHILLIPS Alan John Lander dissanayake asha Janadaree HYDE Kevin David XIANG Meimei 罗梅 Innovative Institute for Plant Health Zhongkai University of Agriculture and Engineering Center of Excellence in Fungal Research Mae Fah Luang University Faculdade de Ciencias Biosystems and Integrative Sciences Institute(BioISI)Universidade de Lisboa School of Life Sciences and Technology University of Electronic Science and Technology of China

Diaporthe species are associated with Citrus as endophytes,pathogens,and saprobes ***,little is known about Diaporthe as endophytes in Citrus grandis in *** this study,24 endophytic Diaporthe isolates were obtained from cultivated *** cv."Tomentosa" in Huazhou,Guangdong Province in *** nuclear ribosomal internal transcribed spacer（ITS）,partial sequences of translation elongation factor 1-a（tef1）,b-tubulin（tub2）,and partial calmodulin（cal） gene regions were sequenced and employed to construct phylogenetic *** on morphology and combined multigene phylogeny,eleven Diaporthe species were identified including two new species,Diaporthe endocitricola and *** are the first report of ***,***,***,***,***,***,***,***,and *** on *** study provides the first intensive study of endophytic Diaporthe species on *** *** in *** results will improve the current knowledge of Diaporthe species associated with *** results obtained in this study will also help to understand the potential pathogens and biocontrol agents and to develop a platform in disease management.

关键词： new host records new species Diaporthales phylogeny taxonomy

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