检索结果-南通市图书馆

A simple and efficient CRISPR/Cas9 system permits ultra-multiplex genome editing in plants

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2024年第2期12卷 569-582页

作者： Suting Wu Htin Kyaw Zhijun Tong Yirong Yang Zhiwei Wang Liying Zhang Lihua Deng Zhiguo Zhang Bingguang Xiao William paul Quick Tiegang Lu Guoying Xiao Guannan Qin Xue'an Cui Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Biological Breeding for Fujian and Taiwan Crops Ministry of Agriculture and Rural AffairsCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125HunanChina School of Biosciences The University of SheffieldFirth CourtWestern BankSheffield S102TNUK Key Laboratory of Tobacco Biotechnological Breeding National Tobacco Genetic Engineering Research CenterYunnan Academy of Tobacco Agricultural SciencesKunming 650021YunnanChina

The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic *** available genome-editing tools have a limited number of targets,restricting their application in genetic *** this study,we developed a novel CRISPR/Cas9 plant ultra-multiplex genome editing system consisting of two template vectors,eight donor vectors,four destination vectors,and one primer-design software *** combining the advantages of Golden Gate cloning to assemble multiple repetitive fragments and Gateway recombination to assemble large fragments and by changing the structure of the amplicons used to assemble sg RNA expression cassettes,the plant ultra-multiplex genome editing system can assemble a single binary vector targeting more than 40 genomic loci.A rice knockout vector containing 49 sg RNA expression cassettes was assembled and a high co-editing efficiency was *** plant ultra-multiplex genome editing system advances synthetic biology and plant genetic engineering.

关键词： CRISPR/Cas9 Multiplex genome editing Assembly system Plant

Cool-season annual pastures with clovers to supplement wintering beef cows nursing calves

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2012年第4期3卷 221-226页

作者： Stacey A Gunter Whitney A Whitworth T Gregory Montgomery paul.a beck United States Department of Agriculture Agricultural Research Service Southern Plains Range Research Station University of Arkansas at Monticello Division of Agriculture Southeast Research & Extension Center University of Arkansas Division of Agriculture Southwest Research & Extension Center University of Arkansas

In December of 3 years, 87 beef cows with nursing calves （.594 ± 9.8 kg; calving season, September to November） at side were stratified by body condition score, body weight, cow age, and calf gender and divided randomly into 6 groups assigned to 1 of 6 cool-season annual pastures （0.45 ha/cow） that had been interseeded into a dormant common bermudagrass （Cynodon dactylon [L.] Pers.）/bahiagrass （Paspalum notatum Flugge） sod. Pastures contained 1 of the following 3 seeding mixtures （2 pastures/mixture）： 1） wheat （Triticum aestivurn L.） and ryegrass （Lofium multiflorum Lain., WRG）, 2） wheat and ryegrass plus red clover （Trifolium pretense L, WRR）, or 3） wheat and ryegrass plus white （Trifofium repens L.） and crimson clovers （Trifolium incarnatum L., WRW）. All groups had ad libitum access to grass hay （12% crude protein; 58% total digestible nutrients）. The second week in December, cow estrous cycles were synchronized and artificially inseminated. In late December, a bull was placed with each group for 60-d. Data were analyzed with an analysis of variance using a mixed model containing treatment as the fixed effect and year as the random effect. Body weight and condition scores did not differ （P ≥ 0.27） among cows between February and June. Calf birth weights or average daily gain did not differ （P≥ 0.17） among treatments; however, calves grazing pastures with clovers did tend （P= 0.06） to weigh more than calves grazing grass only. Weaning weight per cow exposed to a bull was greater （P= 0.02） for WRR and WRW than WRG. Cows grazing winter-annual pastures containing clovers tended to wean more calf body weight per cow exposed to a bull than cows grazing the grass only pastures.

关键词： Annual ryegrass Beef cows Clovers Nitrogen Pasture 1

Experimental Investigation of the Stability of the Performance Characteristics of a Photovoltaic Module in the Face of Environmental and Meteorological Factors

在线全文

学校读者我要写书评

暂无评论

Open Journal of Applied Sciences 2024年第2期14卷 589-608页

作者： Adingra paul.arsène Kouassi Siaka Touré Diakaridja Traoré Laboratoire des Sciences de la Matière de l’Environnement et de l’Energie Solaire (LASMES) Unité de Formation et de Recherche des Sciences des Structures de la Matière et Technologie (UFR-SSMT) Université Félix Houphou&#235t Boigny Abidjan C&#244te d’Ivoire

The explosive technological improvement of photovoltaic systems as well as the necessity of populations to come to less expensive energy sources, that have led to an implosion at the level of solar panel manufacturers. This causes a large flow of these equipments to developing countries where the need is high, without any quality control. That conducted an experimental investigation on the performance characteristics of a 250 wp monocrystalline silicon photovoltaic module in other to check the verification and quality control. Most of these PV panels which often have missing informations are manufactured and tested in places that are inadequate for our environmental and meteorological conditions. Also, their influences on the stability of internal parameters were evaluated in order to optimize their performance. The results obtained at maximum illumination (1000 w/m2) confirmed those produced by the manufacturer. The analysis of these characteristics showed that the illumination and the temperature (meteorological factors) influenced at most the stability of the internal characteristics of the module in the sense that the maximum power increased very rapidly beyond 750 w/m2 but a degradation of performance was accentuated for a temperature of the solar cells exceeding 50°C. The degradation coefficients were evaluated at -0.0864 V/°C for the voltage and at -1.6248 w/°C for the power. The 10° inclination angle of the solar panel proved to be ideal for optimizing overall efficiency in practical situations.

关键词： Renewables Energies Instruments Internal Parameters of Photovoltaic Panel Monocrystalline Photovoltaic Panel Solar Energy Production Energy Intermittence

Spatio-Temporal Change of Dispersal Areas of Greater Kudu (Tragelaphus strepsiceros) in Lake Bogoria Landscape, Kenya

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Ecology 2024年第3期14卷 183-198页

作者： Beatrice Chepkoech Cheserek George Morara Ogendi paul Mutua Makenzi Department of Environmental Science Egerton University Njoro Kenya

Decline in wildlife populations is manifest globally, regionally and locally. A wildlife decline of 68% has been reported in Kenya’s rangelands with Baringo County experiencing more than 85% wildlife loss in the last four decades. Greater Kudu (Tragelaphus strepsiceros) is endemic to Lake Bogoria landscape in Baringo County and constitutes a major tourist attraction for the region necessitating use of its photo on the County’s logo and thus a flagship species. Tourism plays a central role in Baringo County’s economy and is a major source of potential growth and employment creation. The study was carried out to assess spatio-temporal change of dispersal areas of Greater Kudu (GK) in Lake Bogoria landscape in the last four years for enhanced adaptive management and improved livelihoods. GK population distribution primary data collected in December 2022 and secondary data acquired from Lake Bogoria National Game Reserve (LBNGR) for 2019 and 2020 were digitized using in a Geographic Information System (GIS). Measures of dispersion and point pattern analysis (PPA) were used to analyze dispersal of GK population using GIS. Spatio-temporal change of GK dispersal in LBNR was evident thus the null hypothesis was rejected. It is recommended that anthropogenic activities contributing to GK’s habitat degradation be curbed by providing alternative livelihood sources and promoting community adoption of sustainable technologies for improved livelihoods.

关键词： Spatio-Temporal Change Dispersal Greater Kudu (Tragelaphus Strepsiceros) Point Pattern Analysis (PPA) GIS

Investigating the Influence of Glaucoma on the Quality of Life among Adult Patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Clinical Medicine 2024年第2期15卷 88-100页

作者： paul Mwale Karen Tabalasa Abdishakur A. Muse Ke Min Department of Ophthalmology Zhongnan Hospital of Wuhan University Wuhan China

Background: Research exploring the influence of glaucoma on quality of life has gained momentum in recent years. Numerous studies have investigated the multifaceted aspects of quality of life in glaucoma patients, utilizing various assessment tools and methodologies. Objective: To determine the influence that glaucoma has on the quality of life among adult patients. Study site: kitwe teaching eye hospital, Zambia. Method: It was a cross-sectional study that was conducted from 30th June 2022 to 17th April 2023. The study included 106 diagnosed glaucoma patients who were above the age of 55 years. Data was collected using validated 25-item national eye institute visual function Questionnaire (NEI VFQ-25). The data was analyzed using SPSS version 26 and Microsoft excel to present frequencies and percentages that generated graphical presentation. Results: 62% of the participants worry about their eye sight most of the time, 26% sometimes and 12% a little time. 9% of the participants had little difficulty to read ordinary prints, 14% moderate difficulties and 58% extremely difficult. 40% of the participants could drive despite glaucoma damaging their eyes while 60% could not. Majority of the patients said it was difficult noticing things around them while walking or sitted in one place. There was a significant difference between effects of glaucoma on patients and the existing relationship between glaucoma and quality of life (ANOVA, p = 0.019 and ANOVA, p = 0.023). Conclusion: This study has demonstrated the magnitude of impact that glaucoma has on the Quality of Life (QoL) in diagnosed patients and the relationship between glaucoma and the Quality of Life. Due to the progressive visual loss, the amount of activities these patients would perform is negatively affected especially where social and economic activities are concerned which also affects their monthly earnings for those living in middle to low-income countries. Therefore, social welfare a

关键词： Glaucoma Quality of Life Glaucoma Patients

Norian(Late Triassic) conodonts from the Raohe area， Heilongjiang， Northeastern China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Earth Science 2024年

作者： Xianlang Wu paul B.Wignall Xulong Lai Yan Chen Jinling Yuan Guiyue Luo Haishui Jiang State Key Laboratory of Biogeology and Environmental Geology， School of Earth Sciences， China University of Geosciences School of Earth and Environment， University of Leeds Hubei Institute of Geosciences

The Raohe area of Heilongjiang Province， Northeast China belongs to the Nadanhada Terrane， which was in low latitudes of Panthalassa during the Triassic. The composition of the Late Triassic conodont fauna， derived from limestone lenses interpreted to be formed on seamounts， provides important new information on the pelagic biota in this ocean. New conodont samples collected from sections at Minzhu， Minnan and Chigangbei belong to three Norian conodont zones. In ascending order， they are: Mockina postera Zone， Mockina bidentata Zone and Parvigondolella andrusovi Zone. The Norian conodont fauna in the Raohe area has distinct attributes: there are a lot of cosmopolitan species (e.g. Mockina postera， Mockina bidentata， Parvigondolella andrusovi) which enable good global correlation; endemic conodont species are also present， (e.g. Mockina sakurae， Mockina shamiseni， Norigondolella nadanhadaensis) indicating that Panthalassa Ocean conodont populations also contains unique taxa; and some conodonts belong to taxa with much shorter ranges in surrounding epeiric seas (e.g. Carnepigondolella pseudoechinata， Neocavitella cavitata and Epigondolella vialovi). The presence of the latter “relicts” indicates that the seamounts were persistently suitable habitats for many millions of years in the Late Triassic.

关键词：

Heterophase junction engineering-induced Co spin-state modulation of CoSe_(2) for large-current hydrogen evolution reaction

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Rare Metals 2024年第6期43卷 2660-2670页

作者： Bao-Chai Xu Ya-Ping Miao Min-Qin Mao Dong-Lian Li Song Xie Wei-Hong Jin Shu Xiao Jing Wen Zaenab Abd-Allah Zhi-Tian Liu Xiang Peng paul K.Chu Hubei Key Laboratory of Plasma Chemistry and Advanced Materials School of Materials Science and EngineeringWuhan Institute of TechnologyWuhan 430205China School of Textile Science and Engineering Xi'an Polytechnic UniversityXi'an 710048China Institute of Advanced Wear and Corrosion Resistant and Functional Materials Jinan UniversityGuangzhou 510632China Institute of Safety Science and Engineering South China University of TechnologyGuangzhou 510641China Department of Engineering Manchester Metropolitan UniversityManchester M156BHUK Department of Physics Department of Materials Science and Engineeringand Department of Biomedical EngineeringCity University of Hong KongHong Kong 999077China

Efficient electrocatalysts are vital to large-current hydrogen production in commercial water splitting for green energy ***,a novel heterophase engineering strategy is described to produce polymorphic CoSe_(2)*** composition of the electrocatalysts consisting of both cubic CoSe_(2)(c-CoSe_(2))and orthorhombic CoSe_(2)(o-CoSe_(2))phases can be controlled *** results demonstrate that junction-induced spin-state modulation of Co atoms enhances the adsorption of intermediates and accelerates charge transfer resulting in superior large-current hydrogen evolution reaction(HER)***,the CoSe_(2)based heterophase catalyst with the optimal c-CoSe_(2)content requires an overpotential of merely 240 mV to achieve 1,000 mA·cm^(-2)as well as a Tafel slope of 50.4 mV·dec^(-1).Furthermore,the electrocatalyst can maintain a large current density of 1,500 mA·cm^(-2)for over 320 h without *** results reveal the advantages and potential of heterophase junction engineering pertaining to design and fabrication of low-cost transition metal catalysts for large-current water splitting.

关键词： Heterophase junction Spin-state Hydrogen evolution reaction Large current electrochemical hydrogen production Water splitting

Hypertriglyceridemia-induced pancreatitis: A case-based review

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2006年第44期12卷 7197-7202页

作者： S Ian Gan Alun L Edwards Christopher J Symonds paul L beck Division of Gastroenterology and Endocrinology Foothills Hospital University of Calgary Calgary Alberta Canada

Hypertriglyceridemia is an established cause of pancreatitis. In a case-based approach, we present a review of hypertriglyceridemJa and how it can cause pancreatitis. We outline how to investigate and manage such patients. A 35 year old man presented to the emergency department with abdominal pain and biochemical evidence of acute pancreatitis. There was no history of alcohol consumption and biliary imaging was normal. The only relevant past medical history was that of mild hyperlipidemia, treated with diet alone. Physical exam revealed epigastric tenderness, right lateral rectus palsy, lipemia retinalis, bitemporal hemianopsia and a delay in the relaxation phase of his ankle reflexes. Subsequent laboratory investigation revealed marked hypertriglyceridemia and panhypopituarism. An enhanced CT scan of the head revealed a large suprasellar mass impinging on the optic chiasm and hypothalamus. The patient was treated supportively; thyroid replacement and lipid lowering agents were started. He underwent a successful resection of a craniopharyngioma. Postoperatively, the patient did well on hormone replacement therapy. He has had no further attacks of pancreatitis. This case highlights many of the factors involved in the regulation of triglyceride metabolism. We review the common causes of hypertriglyceridemia and the proposed mechanisms resulting in pancreatitis. The incidence and management of hypertriglyceridemiainduced pancreatitis are also discussed.

关键词： Hypertriglyceridemia Pancreatitis Hyperlipidemia

众脑之力:通过开放神经影像数据和大规模协作催化神经精神疾病的创新发现

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science Bulletin 2024年第10期69卷 1536-1555页

作者：鲁彬陈骁 Francisco Xavier Castellanos paul M.Thompson 左西年臧玉峰严超赣 CAS Key Laboratory of Behavioral Science Institute of PsychologyBeijing 100101China Department of Psychology University of Chinese Academy of SciencesBeijing 100101China Department of Child and Adolescent Psychiatry NYU Grossman School of MedicineNew York 10016USA d Nathan Kline Institute for Psychiatric Research Orangeburg 10962USA Imaging Genetics Center Mark&Mary Stevens Institute for Neuroimaging&InformaticsKeck School of MedicineUniversity of Southern CaliforniaLos Angeles 90033USA Developmental Population Neuroscience Research Center IDG/McGovern Institute for Brain ResearchBeijing Normal UniversityBeijing 100875China National Basic Science Data Center Beijing 100190China Centre for Cognition and Brain Disorders The Affiliated Hospital of Hangzhou Normal UniversityHangzhou 310004China Institute of Psychological Science Hangzhou Normal UniversityHangzhou 310030China Zhejiang Key Laboratory for Research in Assessment of Cognitive Impairment Hangzhou 311121China International Big-Data Center for Depression Research Institute of PsychologyChinese Academy of SciencesBeijing 100101China Magnetic Resonance Imaging Research Center Institute of PsychologyChinese Academy of SciencesBeijing 100101China

Recent advances in open neuroimaging data are enhancing our comprehension of neuropsychiatric *** pooling images from various cohorts,statistical power has increased,enabling the detection of subtle abnormalities and robust associations,and fostering new research *** collaborations in imaging have furthered our knowledge of the neurobiological foundations of brain disorders and aided in imaging-based prediction for more targeted ***-scale magnetic resonance imaging initiatives are driving innovation in analytics and supporting generalizable psychiatric *** also emphasize the significant role of big data in understanding neural mechanisms and in the early identification and precise treatment of neuropsychiatric ***,challenges such as data harmonization across different sites,privacy protection,and effective data sharing must be *** proper governance and open science practices,we conclude with a projection of how large-scale imaging resources and collaborations could revolutionize diagnosis,treatment selection,and outcome prediction,contributing to optimal brain health.

关键词： Magnetic resonance imaging Neuropsychiatric disorders Big data Open science Artificial intelligence