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Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation

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synthetic and systems Biotechnology 2019年第1期4卷 57-66页

作者： Lorna suckling Ciaran McFarlane Chelsea sawyer stephen P.Chambers Richard I.Kitney David W.McClymont paul s.freemont The London DNA Foundry SynbiCITEImperial College LondonLondonSW72AZUK Department of Life sciences Imperial College LondonLondonSW72AZUK Department of Bioengineering Imperial College LondonLondonSW72AZUK Department of Medicine Imperial College LondonLondonSW72AZUK

High-throughput preparation of plasmid DNA libraries for next-generation sequencing(NGs)is an important capability for molecular biology *** particular,it is an essential quality control(QC)check when large numbers of plasmid variants are being ***,we describe the use of the Design of Experiments(DOE)methodology to optimise the miniaturised preparation of plasmid DNA libraries for NGs,using the Illumina®Nextera XT technology and the Labcyte Echo®acoustic liquid dispensing ***,we describe methods which can be implemented as a QC check for identifying the presence of genomic DNA(gDNA)in plasmid DNA samples and the subsequent shearing of the gDNA,which otherwise prevents the acoustic transfer of plasmid *** workflow enables the preparation of plasmid DNA libraries which yield high-quality sequencing data.

关键词： preparation otherwise shearing

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Multimode vibrational strong coupling in direct laser written mid-IR plasmonic MIM nano-patch antennas

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Photonics Research 2023年第12期11卷 2136-2148页

作者： NICHOLAs V.PROsCIA MICHAEL A.MEEKER NICHOLAs sHARAC FRANK K.PERKINs CHAsE T.ELLIs paul D.CUNNINGHAM JOsEPH G.TIsCHLER NRC Postdoc residing at U.s.Naval Research Laboratory WashingtonD.C.20375USA U.s.Naval Research Laboratory WashingtonD.C.20375USA Current address:Rigetti Computing FreemontCalifornia 94538USA Current address:Homer L.Dodge Department of Physics and Astronomy The University of OklahomaNormanOklahoma 73019USA

strong coupling of mid-infrared(mid-IR)vibrational transitions to optical cavities provides a means to modify and control a material’s chemical reactivity and offers a foundation for novel chemical detection ***,the relatively large volumes of the mid-IR photonic cavities and weak oscillator strengths of vibrational transitions restrict vibrational strong coupling(VsC)studies and devices to large ensembles of molecules,thus representing a potential limitation of this nascent ***,we experimentally and theoretically investigate the mid-IR optical properties of 3D-printed multimode metal-insulator-metal(MIM)plasmonic nanoscale cavities for enabling strong light-matter interactions at a deep subwavelength *** observe strong vibration-plasmon coupling between the two dipolar modes of the L-shaped cavity and the carbonyl stretch vibrational transition of the polymer *** cavity mode volume is half the size of a typical square-shaped MIM geometry,thus enabling a reduction in the number of vibrational oscillators to achieve strong *** resulting three polariton modes are well described by a fully coupled multimode oscillator model where all coupling potentials are *** 3D printing technique of the cavities is a highly accessible and versatile means of printing arbitrarily shaped submicron-sized mid-IR plasmonic cavities capable of producing strong light–matter interactions for a variety of photonic or photochemical ***,similar MIM structures fabricated with nanoscopic voids within the insulator region could constitute a promising microfluidic plasmonic cavity device platform for applications in chemical sensing or photochemistry.

关键词： coupling vibrational shaped

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Longitudinal analysis of quantitative biomarkers using projection-resolved OCT angiography in retinal vein occlusion

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Annals of Eye science 2021年第2期6卷 19-31页

作者： Alexandra Miere Donato Colantuono Camille Jung Agnès Glacet-Bernard severine Becuwe Eric Petit Eric souied Department of Ophthalmology Centre Hospitalier Intercommunal de CréteilUniversitéParis EstCréteilFrance Laboratory of Images Signals and Intelligent Systems Laboratory(LISSI/EA 3956)University Paris-EstCréteilFrance Clinical Research Center GRC Maculaand Biological Ressources CenterCentre Hospitalier Intercommunal de CréteilCréteilFrance UNIACT NeurospinISVFJCEAGif-sur-YvetteFrance

Background:To evaluate a fully automated vascular density(VD),skeletal density(sD)and fractal dimension(FD)method for the longitudinal analysis of retinal vein occlusion(RVO)eyes using projection-resolved optical coherence tomography angiography(OCTA)images and to evaluate the association between these quantitative variables and the visual prognosis in RVO ***:Retrospective longitudinal observational case *** presenting with RVO to Creteil University Eye Clinic between October 2014 and December 2018 and healthy controls were retrospectively *** 1 consisted of central RVO(CRVO)eyes,group 2 consisted of eyes with branch RVO(BRVO)and group 3 of healthy control *** acquisitions(AngioVue RTVue XR Avanti,Optovue,Inc.,freemont,CA)were performed at baseline and last follow up ***,sD,and FD analysis were computed on OCTA superficial and deep vascular complex(sVC,DVC)images at baseline and final follow up using an automated *** regression was performed to find if and which variable(VD,sD,FD)was predictive for the visual ***:Forty-one eyes,of which 21 consecutive eyes of 20 RVO patients(13 CRVO in group 1,8 BRVO in group 2),and 20 eyes of 20 healthy controls were *** the level of sVC,VD and FD were significantly lower in RVO eyes compared to controls(Pspectively).Best-corrected visual acuity(BCVA)at last follow-up visit was associated with baseline VD(P=0.013),FD(P=0.016),and sD(P=0.01)at the level of the sVC,as well as with baseline FD at the DVC level(P=0.046).Conclusions:Baseline VD,sD,and FD are associated with the visual outcome in RVO *** parameters seem valuable biomarkers and may help improve the evaluation and management of RVO patients.

关键词： Retinal vein occlusion(RVO) vascular density(VD) optical coherence tomography angiography(OCTA) fractal dimension(FD) image analysis

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