检索结果-南通市图书馆

Journal of Genetics and Genomics 2021年第4期48卷 289-299页

作者： Wouter Steyaert Shana Verschuere paul J.Coucke Olivier m.Vanakker Center for medical Genetics Ghent University HospitalGhentBelgium bDepartment of Biomolecular medicine Ghent UniversityGhentBelgium Department of Human Genetics Radboud Institute for Molecular Life SciencesRadboud University Medical CenterNijmegenthe Netherlands

Pseudogenes are frequently encountered noncoding sequences with a high sequence similarity to their protein-coding *** this reason,their presence is often considered troublesome in molecular *** pseudoxanthoma elasticum(PXE),a disease predominantly caused by mutations in ATPbinding cassette family C member 6(ABCC6),the presence of two pseudogenes complicates the analysis of sequence *** whole-exome sequencing(WES)becoming the standard of care in molecular diagnostics,we wanted to evaluate whether this technique is as reliable as gene-specific targeted enrichment analysis for the analysis of *** established a PCR-based targeted enrichment and next-generation sequencing testing approach and demonstrated that the ABCC6-specific enrichment combined with the applied mapping algorithm overcomes the complication of ABCC6 pseudogene aspecificities,contrary to *** propose a time-and cost-efficient diagnostic strategy for comprehensive and accurate molecular genetic testing of PXE,which is highly automatable.

关键词： Pseudoxanthoma elasticum ABCC6 Pseudogenes Next-generation sequencing Whole-exome sequencing Targeted enrichment

On the Contribution of Wu Wen-Tsün to Algebraic Topology

在线全文

学校读者我要写书评

暂无评论

Journal of Systems Science & Complexity 2019年第1期32卷 3-36页

作者： BRASSELET Jean-paul I2m (CNRS)-Aix-marseille University

The aim of this article is to present the contribution of Wu Wen-Tsün to Algebraic Topology and more precisely to the theory of characteristic classes. Several papers provide complete and welldocumented biography and academic career of Wu Wen-Tsün, in particular, Hudecek, 2014; O'Connor and Robertson, 2006; Wen-Tsün Wu's Academic Career, 2006; Selected works of Wen-Tsun Wu, *** author does not repeat the details provided in these papers concerning the Wu Wen-Tsün's bibliography, we will just mention people involved in the Wu Wen-Tsün's period in *** addition to Wu Wen-Tsün's papers, the Dieudonné's book(Dieudonné, 1960) provides an excellent presentation of main results of Wu Wen-Tsün in Algebraic and Differential Topology. The author will use and abuse of this book(and refer to) when *** the introduction, the author recalls mainly historical facts concerning the contribution of Wu Wen-Tsün to Algebraic Topology. The second section shows speci?cally the contribution of Wu WenTsün to the Stiefel-Whitney classes and introduces the third section, dealing with the(real) Wu *** author provides de?nition, properties as well as further developments and generalizations of the Wu classes. The fourth and ?fth sections are devoted to recent applications: In Cobordism theory and in mathematical Physics. The author notices that Wu classes have been used as well in other domains,in particular surgery theory(madsen and milgram, 1979). The last section concerns the complex Wu classes and shows that the more recent mather classes coincide with the previously de?ned complex Wu classes, that is a result from Zhou(1994)(see also Liu, 1996).This article is devoted to the contribution of Wu Wen-Tsün to the theory of Characteristic Classes,which coincides with his "French period"(1947–1951). However, speaking of Algebraic Topology, it is worthwhile to mention the important contribution of Wu Wen-Tsün to the Theory of realization of complexes or manifolds in Eucl

关键词： Algebraic topology characteristic class cobordism theory mathematical physics Wu Class

Combined administration of platelet rich plasma and autologous bone marrow aspirate concentrate for spinal cord injury: a descriptive case series

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2021年第2期16卷 362-366页

作者： Joseph A.Shehadi Steven m.Elzein paul Beery m.Chance Spalding michelle Pershing Section of Neurosurgery at OhioHealth Grant medical Center Cedar Stem Cell InstituteColumbusOHUSA The Ohio State University College of medicine ColumbusOHUSA Division of Trauma and Acute Care Surgery OhioHealth Grant Medical CenterColumbusOHUSA OhioHealth Research Institute ColumbusOHUSA

Administration of platelet rich plasma(PRP) and bone marrow aspirate concentrate(BmAC) has shown some promise in the treatment of neurological conditions;however, there is limited information on combined administration. As such, the purpose of this study was to assess safety and functional outcomes for patients administered combined autologous PRP and BmAC for spinal cord injury(SCI). This retrospective case series included seven patients who received combined treatment of autologous PRP and BmAC via intravenous and intrathecal administration as salvage therapy for SCI. Patients were reviewed for adverse reactions and clinical outcomes using the Oswestry Disability Index(ODI) for up to 1 year, as permitted by availability of follow-up data. Injury levels ranged from C3 through T11, and elapsed time between injury and salvage therapy ranged from 2.4 months to 6.2 years. Post-procedure complications were mild and rare, consisting only of self-limited headache and subjective memory impairment in one patient. Four patients experienced severe disability prior to PRP combined with BmAC injection, as evidenced by high(> 48/100) Oswestry Disability Index scores. Longitudinal Oswestry Disability Index scores for two patients with incomplete SCI at C6 and C7, both of whom had cervical spine injuries, demonstrated a decrease of 28–40% following salvage therapy, representing an improvement from severe to minimal disability. In conclusion, intrathecal/intravenous co-administration of PRP and BmAC resulted in no significant complications and may have had some clinical benefits. Larger clinical studies are needed to further test this method of treatment for patients with SCI who otherwise have limited meaningful treatment options. This study was reviewed and approved by the Ohio Health Institutional Review Board(IRB No. 1204946) on may 16, 2018.

关键词： bone marrow aspirate concentrate cell-based therapy neural regeneration Oswestry Disability Index platelet rich plasma spinal cord injury stem cells

Red cell transfusion trigger-avoiding the highs and the lows

在线全文

学校读者我要写书评

暂无评论

中国输血杂志 2010年第10期23卷 834-835页

作者： paul m.Ness Transfusion medicine Division Johns Hopkins Medical InstitutionsBaltimore Maryland USA

One of the most vexing problems in AIHA is handling the acute situation where all blood is incompatible and the patient has severe,worsening anemia. Since the panagglutinin in the patient's serum typically reacts with all donor red cells,crossmatching donor blood is a difficult and time consuming process and probably of little benefit. The most pressing problem in a patient with previous pregnancies or transfusions is detecting alloantibody which may be hidden by the autoantibody. Sophisticated immunohematology laboratories can use a combination of procedures including differential adsorption and warm autoadsorption to identify underlying alloantibodies.

关键词：输血红细胞触发自身抗体工业卫生细胞反应抗体检测血液学

自牺牲型金属有机框架衍生In_(2)S_(3)多级孔结构纳米材料强化光催化性能

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Catalysis 2024年第4期59卷 204-213页

作者：杨婷婷王彬朱剑豪夏杰祥李华明江苏大学化学化工学院能源研究院江苏镇江212013 香港城市大学物理系材料科学与工程系生物医学工程系中国香港

半导体基光催化是减少对化石燃料的依赖和解决环境污染问题的有前景的策略之一.在光催化有机污染物降解领域,硫化物材料备受关注.其中,In_(2)S_(3)催化剂因展现较好的催化活性及经济可行性,而被认为是光催化降解的理想催化剂之一.然而,... 详细信息

半导体基光催化是减少对化石燃料的依赖和解决环境污染问题的有前景的策略之一.在光催化有机污染物降解领域,硫化物材料备受关注.其中,In_(2)S_(3)催化剂因展现较好的催化活性及经济可行性,而被认为是光催化降解的理想催化剂之一.然而,采用传统煅烧法制备的In_(2)S_(3)催化剂由于比表面积小,暴露的活性位点有限,进而限制了其催化活性的进一步提升.因此,制备具有较大比表面积和多活性位点的In_(2)S_(3)基催化剂,对于推动光催化降解有机污染物领域的发展具有重要的意义.本文通过构建分级多孔结构的光催化剂,以增强材料的光吸收性能并优化光生载流子的迁移和分离,从而提升光催化降解有机污染物的性能.利用In基金属有机骨架(mOFs)作为自我牺牲模板,通过硫化制备了包括空心纳米管、微管、中空球和十二面体在内的多种分级In_(2)S_(3)光催化剂.分级多孔结构不仅增强了入射光的多次折射和反射,还提供了更大的表面积,从而提高了光生载流子的光利用率和相分离效率.实验结果表明,这些材料的光催化效率远高于块状和商用In_(2)S_(3).通过X射线光电子能谱、X射线衍射等手段验证了不同形貌分级多孔In_(2)S_(3)材料的成功制备.紫外-可见漫反射光谱结果表明,所有催化剂均可吸收部分可见光,结合莫特肖特基曲线和XPS价带谱结果,说明催化剂的导带、价带位置均满足光催化降解有机污染物的要求.光致发光光谱、光电流强度曲线和电化学阻抗曲线等研究表明,分级多孔结构有效促进了光生载流子的分离和迁移.光催化降解罗丹明B(RhB)和四环素(TC)性能评价结果表明,与块状和商用In_(2)S_(3)相比,具有分级多孔结构的In_(2)S_(3)材料表现出更好的光催化降解活性.其中,空心In_(2)S_(3)纳米管(HNTs)具有最佳的光催化性能,在光照1.5和2 h后,In_(2)S_(3)-HNT可以去除约50%的TC和95%的RhB,其RhB的降解速率分别是块状和商业In_(2)S_(3)的135.6倍和446.9倍,TC的降解速率分别是块状和商业级In_(2)S_(3)的7.8倍和36.5倍.电子顺磁共振和自由基捕获实验结果表明,在光催化降解过程中,空穴、超氧自由基以及单线态氧是主要的活性物种.特别是,当In_(2)S_(3)-HNT受到光照时,其独特的分级多孔结构使得光生电子和空穴对能够有效分离,这使得In_(2)S_(3)-HNT可以积累更多的活性氧自由基,从而显著提升了其光催化降解有机污染物的性能.综上,本文采用新型自牺牲模板法,制备了金属有机框架衍生In_(2)S_(3)多级孔结构纳米材料.通过精准调控In_(2)S_(3)催化剂的形貌,有效提升了光催化降解有机污染物性能.该方法在高效光催化剂的制备上展现了显著潜力,为设计高性能的光催化降解材料提供参考.

关键词：硫化铟金属有机框架形貌调控光催化污染物降解

Ferritin acts as a target site for the snowdrop lectin （GNA） in the midgut of the cotton leafworm Spodoptera littoralis

在线全文

学校读者我要写书评

暂无评论

Insect Science 2008年第6期15卷 513-519页

作者： Amin Sadeghi Guy Smagghe paul Proost Els J.m. Van Damme Laboratory of Agrozoology Department of Crop Protection Laboratory of Biochemistry and Glycobiology Department of Molecular Biotechnology Faculty of Bioscience Engineering Ghent University Ghent Rega Institute Laboratory of Molecular Immunology Kathofieke Universiteit Leuven Minderbroedersstraat 10 B-3000 Leuven Belgium

The snowdrop lectin GNA （Galanthus nivalis agglutinin） has been shown to possess insecticidal activity to a range of economically important insect pests. However, the precise mechanism of insecticidal action of GNA against insects remains unknown. In this investigation, we attempted to purify and identify receptor（s） responsible for binding of GNA in the larval midgut of a major lepidopteran pest （the cotton leafworm, Spodoptera littoralis） to better understand its mode of action. Therefore, cytoplasmic as well as membrane proteins from 800 larval midguts were chromatographed on a column with immobilized GNA. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the proteins eluted from the GNA column followed by sequencing of the GNA-binding proteins and BLAST analyses revealed that the N-terminal sequences of a 24 kDa polypeptide purified from the cytoplasmic and membrane protein fraction revealed sequence similarity to sequences encoding heavy chain homologs of ferritin from manduca sexta （76% sequence identity）, Calpodes ethlius （80% sequence identity） and Bombyx mori （61% sequence identity）. Furthermore, the N-terminal sequence of a 31 kDa polypeptide from the membrane protein fraction showed sequence similarity to a light chain homolog of ferritin from manduca sexta （88% sequence identity）.

关键词： ferritin Galanthus nivalis agglutinin GNA Lepidoptera midgut receptors Spodoptera littoralis

A nonparametric estimation of the infection curve

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China mathematics 2011年第9期54卷 1815-1828页

作者： LIN HuaZhen YIP paul S. F HUGGINS Richard m School of Statistics Southwestern University of Finance and Economics Chengdu 611130 China Social Work and Social Administration University of Hong Kong Hong Kong China Department of mathematics and Statistics The University of Melbourne Melbourne Australia

Predicting the future course of an epidemic depends on being able to estimate the current numbers of infected ***,while back-projection techniques allow reliable estimation of the numbers of infected individuals in the more distant past,they are less reliable in the recent *** propose two new nonparametric methods to estimate the unobserved numbers of infected individuals in the recent past in an *** proposed methods are noniterative,easily computed and asymptotically normal with simple variance *** show that the proposed methods are much more robust and accurate than the existing back projection method,especially for the recent past,which is our primary *** apply the proposed methods to the 2003 Severe Acute Respiratory Syndorme(SARS) epidemic in Hong Kong.

关键词： epidemic back-projection nonparametric method infection curve

An assessment of the taxonomy and chemotaxonomy of Ganoderma

在线全文

学校读者我要写书评

暂无评论

Fungal Diversity 2015年第2期71卷 1-15页

作者： Christian Richter Kathrin Wittstein paul m.Kirk marc Stadler Department microbial Drugs Helmholtz Centre for Infection Research GmbHInhoffenstraße 738124 BraunschweigGermany mycology Section Royal Botanic GardensKewSurrey TW93DSUK State Key Laboratory of mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China

The taxonomic and nomenclatural history of the genus Ganoderma and related basidiomycetes is reviewed and compared to recent studies on its molecular phylogeny.A basidiomycete belonging to the genus Ganoderma can often rather easily be recognised in the field from the macromorphological characters of the *** most important species and lineages can also be discriminated well by molecular ***,the application of incongruent species concepts and the frequent misapplication of European names by chemists and other non-taxonomists have created confusion in the scientific *** identity of the species reported in the course of mycochemical studies can often not be verified,since no voucher material was *** this review,an overview on the most important types of specific chemotaxonomic traits(i.e.,secondary metabolites of the basidiomes and mycelia)reported from the genus is *** certain triterpenoids such as ganoderic and lucidenic acids,steroids(***)and triterpenes(***)appear to have some chemotaxonomic value at the generic rank,their relevance for species discrimination remains to be *** propose that all important names in Ganoderma should be,as required,epitypified by fresh collections for which living cultures should be made available and that these should be examined by a combination of morphological,chemotaxonomic and molecular phylogenetic methods to attain a more stable taxonomy.

关键词： Polyporales Basidiomycota Chemosystematics Evolution

Real-time seismology for the 05/12/2008 Wenchuan earthquake of China: A retrospective view

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Earth Sciences 2009年第2期52卷 155-165页

作者： paul SOmmERVILLE United Research Services Corporation

The devastating 05/12/2008 Wenchuan earthquake (mw7.9) in Sichuan Province of China showed very few precursory phenomena and occurred on a fault system once assigned to be of moderate long term seismic risk. Given the existing coverage of seismograph stations in Sichuan Province, real-time seis-mology could have been effective in avoiding some earthquake damage and helping post-earthquake emergency response. In a retrospective view, we demonstrated that the epicenter can be located with 20 km accuracy using just two broadband stations with three-component, which takes only about 10 s after the onset of the earthquake. Initial magnitude is estimated to be m7 with the Tc measurement over first 4 seconds of P waves. Better magnitude estimate can be obtained within 2 min by modeling Pnl waves for stations about 500 km away where the S waveforms are clipped. The rupture area is well revealed by teleseismically-recorded >m5 early aftershocks within two hours after the mainshock. Within a few hours, teleseismic body waves were inverted to derive a more detailed rupture process and the finite fault model can be readily used to calculate ground motions, thus providing vital information for rescue efforts in the case where no real-time strong motion records are available.

关键词： real-time seismology earthquake early warning strong ground motion rapid location

Spatiotemporal characteristics of positive cloud-to-ground lightning discharges and bidirectional leader of the lightning

在线全文

学校读者我要写书评

暂无评论

Science China Earth Sciences 2006年第2期49卷 212-224页

作者： Krehbiel paul New mexico Institute of mining and Technology Socorro New Mexico 87801 USA

The three-dimension spatiotemporal development characteristics of positive cloud-to- ground (CG) lightning discharges have been analyzed by using the data measured by the lightning mapping array system with high time and space resolution. The results indicate that a positive CG lightning discharge can be divided into three stages based on the characteristics of its development. The first stage is discharge process in cloud with a long duration preceding the return stroke. This process with an average of 370 ms propagated at velocity of 105 m/s and produced intensive radiation with a magnitude equal to that of the negative leader. During this stage, the lightning channels de-veloped horizontally in the positive charge region with few branches as the negative polarity break-down. During the stage after the return stroke of the positive CG lightning, the lightning channels propagated at velocity of 2 times faster than that before the return stroke. This stage involved lots of positive fast impulses and corresponded to the continuing current process producing less and dis-persed radiation points and more intensive radiation powers. During the final stage of the positive CG lightning, the lightning channels developed at velocity equal to that before the return stroke and the radiation points appeared mainly at the ends of channel. The spatiotemporal development character-istics of the positive CG lightning are very different from that of the negative CG lightning. All of the radiation points of the positive CG lightning appeared in the positive charge region of cloud. Little or no radiation was detected during the positive leader just before the return stroke. The duration of the positive CG lightning was an average of 730 ms. The positive CG lightning discharges with lasting time of 500―600 ms were 43%. 90% of the positive CG lightning discharges involved one return stroke, the most return stroke number being four. The current of the return stroke was an average of 36.5

关键词： positive CG lightning, radiation characteristics, bidirectional leader.