检索结果-南通市图书馆

Quantitative and qualitative subgenomic RNA profiles of SARS-CoV-2 in respiratory samples: A comparison between Omicron BA.2 and non-VOC-D614G

引用

Virologica Sinica 2024年第2期39卷 218-227页

作者： Zigui Chen Rita Way Yin Ng Grace Lui Lowell Ling Agnes S.Y.Leung Chit Chow Siaw Shi Boon Wendy C.S.Ho Maggie Haitian Wang Renee Wan Yi chan Albert Martin Li David Shu Cheong Hui paul kay sheung chan Department of Microbiology Faculty of MedicineThe Chinese University of Hong KongHong KongChina Department of Medicine and Therapeutics Faculty of MedicineThe Chinese University of Hong KongHong KongChina Department of Anaesthesia and Intensive Care Faculty of MedicineThe Chinese University of Hong KongHong KongChina Department of Paediatrics Faculty of MedicineThe Chinese University of Hong KongHong KongChina Department of Anatomical and Cellular Pathology Faculty of MedicineThe Chinese University of Hong KongHong KongChina Jockey Club School of Public Health and Primary Care Faculty of MedicineThe Chinese University of Hong KongHong KongChina Stanley Ho Centre for Emerging Infectious Diseases Faculty of MedicineThe Chinese University of Hong KongHong KongChina

The SARS-CoV-2 Omicron variants are notorious for their transmissibility,but little is known about their subgenomic RNA(sgRNA)*** study applied RNA-seq to delineate the quantitative and qualitative profiles of canonical sgRNA of 118 respiratory samples collected from patients infected with Omicron BA.2 and compared with 338 patients infected with non-variant of concern(non-VOC)-D614G.A unique characteristic profile depicted by the relative abundance of 9 canonical sgRNAs was reproduced by both BA.2 and non-VOCD614G regardless of host gender,age and presence of ***,such profile was lost in samples with low viral load,suggesting a potential application of sgRNA pattern to indicate viral activity of individual patient at a specific time point.A characteristic qualitative profile of canonical sgRNAs was also reproduced by both BA.2 and *** presence of a full set of canonical sgRNAs carried a coherent correlation with crude viral load(AUC¼0.91,95%CI 0.88–0.94),and sgRNA ORF7b was identified to be the best surrogate marker allowing feasible routine application in characterizing the infection status of individual *** potentials in using sgRNA as a target for vaccine and antiviral development are worth pursuing.

关键词： Coronavirus SARS-CoV-2 Omicron BA.2 Non-VOC-D614G RNA-seq Subgenomic RNA(sgRNA)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Clinical significance of hepatic derangement in severe acute respiratory syndrome

引用

World Journal of Gastroenterology 2005年第14期11卷 2148-2153页

作者： Henry Lik-Yuen chan Ambrose Chi-Pong Kwan Ka-Fai To Sik-To Lai paul kay-sheung chan Wai-Keung Leung Nelson Lee Alan Wu Joseph Jao-Yiu Sung Department of Medicine and Therapeutics Chinese University of Hong Kong Hong Kong SARChina Department of Medicine Princess Margaret Hospital Hong Kong SARChina Department of Anatomical and Cellular Pathology Chinese University of Hong Kong Hong Kong SARChina Department of Microbiology Chinese University of Hong Kong Hong Kong SARChina

AIM:Elevation of alanine aminotransferase (ALT) level is commonly seen among patients suffering from severe acute respiratory syndrome (SARS). We report the progression and clinical significance of liver derangement in a large cohort of SARS patient. METHODS: Serial assay of serum ALT was followed in patients who fulfilled the WHO criteria of SARS. Those with elevated ALT were compared with those with normal liver functions for clinical outcome. Serology for hepatitis B virus (HBV) infection was checked. Adverse outcomes were defined as oxygen desaturation, need of intensive care unit (ICU) and mechanical ventilation and death. RESULTS: Two hundred and ninety-four patients were included in this study. Seventy (24%) patients had elevated serum ALT on admission and 204 (69%) patients had elevated ALT during the subsequent course of illness. Using peak ALT >5xULN as a cut-off and after adjusting for potential confounding factors, the odds ratio of peak ALT >5x ULN for oxygen desaturation was 3.24 (95%CI 1.23-8.59, P= 0.018), ICU care was 3.70 (95%CI 1.38-9.89, P= 0.009), mechanical ventilation was 6.64 (95%CI 2.22-19.81, P = 0.001) and death was 7.34 (95%CI 2.28-24.89, P= 0.001). Ninety-three percent of the survived patients had ALT levels normalized or were on the improving trend during follow-up. Chronic hepatitis B was not associated with worse clinical outcomes. CONCLUSION: Reactive hepatitis is a common complication of SARS-coronavirus infection. Those patients with severe hepatitis had worse clinical outcome.

关键词： SARS Hepatitis Hepatitis B virus Coronavirus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Anti-inflammatory mechanisms of the novel cytokine interleukin-38 in allergic asthma

引用

Cellular & Molecular Immunology 2020年第6期17卷 631-646页

作者： Xiaoyu Sun Tianheng Hou Edwin Cheung Tiffany Nga-Teng Iu Victor Wai-Hou Tam Ida Miu-Ting Chu Miranda Sin-Man Tsang paul kay-sheung chan Christopher Wai-Kei Lam Chun-Kwok Wong Department of Chemical Pathology The Chinese University of Hong KongHong KongChina Cancer Centre/Centre of Precision Medicine Research and Training Faculty of Health SciencesUniversity of MacaoMacaoChina Institute of Chinese Medicine and State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants The Chinese University of Hong KongHong KongChina Department of Microbiology The Chinese University of Hong KongHong KongChina Faculty of Medicine and State Key Laboratory of Quality Research in Chinese Medicines Macao University of Science and TechnologyMacaoChina Li Dak Sum Yip Yio Chin R&D Centre for Chinese Medicine The Chinese University of Hong KongHong KongChina

We elucidated the anti-inflammatory mechanisms of IL-38 in allergic *** bronchial epithelial cells and eosinophils were cocultured upon stimulation with the viral RLR ligand poly(I:C)/LyoVec or infection-related cytokine TNF-αto induce expression of cytokines/chemokines/adhesion *** dust mite(HDM)-induced allergic asthma and humanized allergic asthma NOD/SCID murine models were established to assess anti-inflammatory mechanisms in ***-38 significantly inhibited induced proinflammatory IL-6,IL-1β,CCL5,and CXCL10 production,and antiviral interferon-βand intercellular adhesion molecule-1 expression in the coculture *** cytometry and RNA-sequencing analysis revealed that IL-38 could antagonize the activation of the intracellular STAT1,STAT3,p38 MAPK,ERK1/2,and NF-κB pathways,and upregulate the expression of the host defense-related gene POU2AF1 and anti-allergic response gene *** injection of IL-38 into HDM-induced allergic asthma mice could ameliorate airway hyperreactivity by decreasing the accumulation of eosinophils in the lungs and inhibiting the expression of the Th2-related cytokines IL-4,IL-5,and IL-13 in the bronchoalveolar lavage fluid(BALF)and lung *** examination indicated lung inflammation was alleviated by reductions in cell infiltration and goblet cell hyperplasia,together with reduced Th2,Th17,and innate lymphoid type 2 cell numbers but increased proportions of regulatory T cells in the lungs,spleen,and lymph ***-38 administration suppressed airway hyperreactivity and asthma-related IL-4 and IL-5 expression in humanized mice,together with significantly decreased CCR3^(+) eosinophil numbers in the BALF and lungs,and a reduced percentage of human CD4^(+)CRTH2^(+)Th2 cells in the lungs and mediastinal lymph ***,our results demonstrated the anti-inflammatory mechanisms of IL-38 and provided a basis for the development of a regulatory cytokine-based treatment for allergic

关键词： allergic asthma eosinophils IL-38 ILC2s Tregs

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

展枝马尾藻硫酸多糖对呼吸道合胞病毒的抗病毒活性及其机理研究(英文)

引用

中国海洋药物 2006年第1期25卷 32-38页

作者：朱文黄荣春伍泽庚陈基湘四川大学华西医院肺癌分子实验室四川成都610041 香港中文大学生物系新界香港中文大学微生物系新界

目的研究从褐藻展枝马尾藻Sargassumpatens中分离到的一种硫酸多糖SP2的抗病毒活性及抗病毒作用机理。方法通过细胞致病效应实验、MTT测定和空斑形成抑制实验对该多糖的抗病毒活性、细胞毒性和可能的抗病毒机理进行测定。结果通过空斑... 详细信息

目的研究从褐藻展枝马尾藻Sargassumpatens中分离到的一种硫酸多糖SP2的抗病毒活性及抗病毒作用机理。方法通过细胞致病效应实验、MTT测定和空斑形成抑制实验对该多糖的抗病毒活性、细胞毒性和可能的抗病毒机理进行测定。结果通过空斑形成抑制实验测定结果显示,该多糖对呼吸道合胞病毒有好的抗病毒活性,其EC50值为1.1但没有抗流感病毒A和B、副流感病毒的活性。多糖的CC50高达3000μ***-1。其对呼吸道合胞病毒的抗病毒选择性指数被估计大于2727。用SP2预处理宿主细胞,不显示任何对病毒感染的保护作用。SP2对呼吸道合胞病毒有一定的杀病毒活性,其EC50值超过100μ***-1。时间加入实验证明,只有在37℃呼吸道合胞病毒吸附时期加入SP2才能完全抑制病毒的复制。结论SP2可能是一种有潜力的抗呼吸道合胞病毒感染的多糖。其对呼吸道合胞病毒的抗病毒作用机制与抑制病毒的吸附有关。

关键词：展枝马尾藻硫酸多糖抗病毒活性呼吸道合胞病毒

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identification of pathologic features associated with “ulcerative colitis-like” Crohn's disease

引用

World Journal of Gastroenterology 2014年第36期20卷 13139-13145页

作者： Samuel D James paul E Wise Tania Zuluaga-Toro David A Schwartz M kay Washington chanjuan Shi Departments of Pathology Microbiology and Immunology Vanderbilt University Medical Center Nashville TN 37232 United States Section of Colon and Rectal Surgery Washington University in St. Louis Gainesville MO 63130 United States Department of Pathology University of Florida Gainesville FL 32611 United States Departments of Gastroenterology Hepatology and Nutrition Vanderbilt University Medical Center Nashville TN 37232 United States

AIM: To identify pathologic features associated with this “ulcerative colitis (UC)-like” subgroup of Crohn’s disease (CD).

关键词： Ulcerative colitis-like Crohn’ s disease Ulcerative colitis Crohn’ s colitis Inflammatory bowel disease

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Comparative Assessment of the Surface Roughness of Thermoplastic Denture Base Resins Following Adjustment and Re-Polishing

引用

Open Journal of Stomatology 2017年第4期7卷 250-263页

作者： Shivaughn M. Marchan Anna kay Bishop William A. J. Smith paul Seerattan David Hinds Unit of Restorative Dentistry School of Dentistry Faculty of Medical Sciences The University of the West Indies St Augus-tine Trinidad and Tobago School of Dentistry Faculty of Medical Sciences the University of the West Indies St Augustine Trinidad and Tobago Department of Physics Faculty of Science and Technology the University of the West Indies St Augustine Trinidad and Tobago

Purpose: This study assessed the roughness of two injection-molded, thermoplastic materials used for denture bases compared with a polyamide material and compression molded Polymethylmethacrylate (PMMA) after the adjustment and re-polishing with either a laboratory protocol or a chair side protocol. Methods: Forty specimens, each of PMMA, Valplast, DuraFlex, Dura Cetal were fabricated and finished according to individual manufactures’ instructions. These materials were adjusted with tungsten carbide (TC) burs to mimic gross adjustments, and then re-polished either on a lathe or bonded silicon carbide (B-SC). Following instrumentation, the specimens were assessed using contact profilometry and scanning electron microscopy. Two-factor ANOVA was used to determine significant differences in mean surface roughness (Ra and Rmax), with included factors being material type and re-polishing regimen. Results: Mean Ra values ranged from 0.26 (DuraFlex control) to 1.82 (Valplast adjusted with TC burs). Mean Rmax values ranged from 1.88 (Dura Flex control) to 13.76 (Valplast adjusted with TC burs). Two-factor ANOVA revealed that interaction of both factors was significant (p Ra and Rmax. There was a statistically significant increase in both Ra (p Rmax (p < 0.05) for all material types following the gross adjustment. With the exception of DuraFlex, re-polishing of samples that were previously adjusted with TC burs, on the dental lathe produced surfaces that were comparable to control samples. Conclusion: Adjustment of DuraFlex should be kept to a minimum since the adjustment produced the significant surface detriment that could not be corrected with either of the polishing regimens.

关键词： DuraFlex DuraCetal Valplast Poly Methylmethacrylate Surface Roughness

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

奥古斯丁

引用

2019年

作者： (美)沙伦·M. 凯(Sharon M. kay)，(美)保罗·汤姆森(paul Thomson)

来源：南通市图书馆图书评论

学校读者我要写书评

暂无评论

B&K界定基本颜色术语的语言学诠释

引用

曲靖师范学院学报 2007年第5期26卷 80-84页

作者：保罗.凯依朱宏华美国加州大学伯克利分校曲靖师范学院外语学院云南曲靖655011

人们对颜色词汇的语言学研究一直关注把语言的"基本"颜色词语分离出来:说话者可以说出任何一个颜色词语的一种语言学层面的最小一组的简单语言表达。

关键词：语言学 B&K 基本颜色术语定义

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

奥古斯丁

引用

作者： (美)沙伦·M. 凯(Sharon M. kay)，(美)保罗·汤姆森(paul Thomson)

来源：南通市图书馆图书评论

学校读者我要写书评

暂无评论

EPIC-Norfolk眼病研究中的青光眼和眼压:横断面研究

引用

英国医学杂志中文版 2019年第3期22卷 134-134页

作者： Michelle P Y chan David C Broadway Anthony P Khawaja David F Garway- Heath Jennifer M Burr Robert Luben Shabina Hayat Nichola Dalzell kay-Tee Khaw paul J Foster 杨一佺(译) 不详首都医科大学附属北京同仁医院眼科

目的报告眼压随年龄和性别的分布情况与青光眼的患病率。方法社区人群为基础的横截面观察性研究。背景诺维奇(Norwich)城区和郊区的EPIC-Norfolk队列研究。参与者从社区招募的8623名年龄在48~92岁的参与者,为其进行青光眼相关的眼科检... 详细信息

目的报告眼压随年龄和性别的分布情况与青光眼的患病率。方法社区人群为基础的横截面观察性研究。背景诺维奇(Norwich)城区和郊区的EPIC-Norfolk队列研究。参与者从社区招募的8623名年龄在48~92岁的参与者,为其进行青光眼相关的眼科检查。主要结局测量青光眼患病率及其特点,眼压的分布情况,眼压诊断青光眼的敏感性与特异性。

关键词：青光眼眼压横断面眼病社区人群队列研究眼科检查患病率

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：