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Dismantling Melaspileaceae:a first phylogenetic study of Buelliella,Hemigrapha,Karschia,Labrocarpon and Melaspilea

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Fungal Diversity 2015年第2期71卷 141-164页

作者： damien Ertz paul Diederich Department Bryophytes-Thallophytes(BT) Botanic Garden MeiseNieuwelaan 38B–1860 MeiseBelgium Musée national d’histoire naturelle 25 rue MunsterL–2160 LuxembourgLuxembourg

Melaspileaceae is a heterogeneous group of Ascomycota including lichenized,lichenicolous and saprobic fungi.A first phylogenetic study of Melaspileaceae is presented and is based on mtSSU and nuLSU sequence *** obtained 49 new sequences for 28 specimens representing 15 *** genera Buelliella,Hemigrapha,Karschia,Labrocarpon and Melaspilea *** included in a molecular phylogeny for the first *** is recovered as polyphyletic,with members placed in two main lineages of *** *** included in *** is placed in synonymy with *** genus Encephalographa is placed in *** genera Buelliella,Karschia,Labrocarpon and several members of Melaspilea are demonstrated to belong to Asterinales,while Hemigrapha is confirmed in this *** genera Melaspileella,Melaspileopsis,Stictographa are reinstated for former Melaspilea species now placed in *** cezannei is described as new,and the new combinations Melaspilea costaricensis,***,***,Melaspileella proximella and Melaspileopsis diplasiospora are *** as newly defined includes lichenized and saprobic *** lichenicolous and saprobic life styles form different intermixed lineages in Asterinales that do not include lichenized *** phylogenetic data provide a first framework for dismantling further the genus Melaspilea for which most of the species are expected to belong to Asterinales.

关键词： Asterinales Eremithallales Lichenicolous fungi Phylogeny Taxonomy

Multiple origin of the lichenicolous life habit in Helotiales,based on nuclear ribosomal sequences

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Fungal Diversity 2015年第1期70卷 55-72页

作者： Ave Suija damien Ertz James D.Lawrey paul Diederich Institute of Ecology and Earth Sciences University of Tartu40 Lai st.EE-51005 TartuEstonia Département Bryophytes et Thallophytes Jardin botanique national de BelgiqueDomaine de BouchoutB-1860 MeiseBelgium Department of Environmental Science and Policy George Mason UniversityMSN 5G810900 University BoulevardManassasVA 20110-2203USA Musée national d’historie naturelle 25 rue MunsterL-2160 LuxembourgLuxembourg

The Helotiales are an ecologically and morphologically highly diverse group of ascomycetes that also includes lichen-inhabiting(lichenicolous)*** generated sequence data of three rDNA regions(nuSSU,nuLSU,5.8S of ITS)from 28 lichenicolous specimens representing nine genera in order to determine their phylogenetic *** on the most complete dataset of helotialean fungi to date,the analyses were performed using Maximum Likelihood(ML)and Bayesian *** results suggest that 1)the lichen-inhabiting life-style in Helotiales was subjected to gains or losses at least three times;2)Thamnogalla,previously tentatively included in Ostropales,is shown to belong to Helotiales;3)ascomata found intermixed with pycnidia of the asexual Diplolaeviopsis ranula and possessing the same pigments are tentatively considered as the sexual morph of this taxon,and are shown to belong to Helotiales;4)the lichenicolous species of ***,Llimoniella,Rhymbocarpus,Skyttea,Thamnogalla and Unguiculariopsis form a well-supported clade together with non-lichenicolous encoelioid fungi from the genera Ionomidotis,Cordierites and Encoelia;5)Geltingia associata forms a highly supported clade with the fungicolous asexual fungus Eleutheromyces subulatus,whereas Pezizella epithallina clusters with an aquatic asexual fungus Tetracladium sp.;6)Phaeopyxis punctum belongs to Ostropomycetidae(Lecanoromycetes),but its deep relationships to other groups remain unresolved based on rDNA sequences.

关键词： Convergence Helotiales rDNA Taxonomy Diplolaeviopsis Geltingia Llimoniella Pezizella Phaeopyxis Rhymbocarpus Skyttea Thamnogalla Unguiculariopsis

Molecular data resolve a new order of Arthoniomycetes sister to the primarily lichenized Arthoniales and composed of black yeasts, lichenicolous and rock-inhabiting species

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Fungal Diversity 2014年第3期66卷 113-137页

作者： damien Ertz James D.Lawrey Ralph S.Common paul Diederich Jardin botanique national de Belgique Domaine de BouchoutB-1860 MeiseBelgium Department of Environmental Science and Policy George Mason University4400 University DriveFairfaxVA 22030-4444USA 534 Fenton St LansingMI 48910USA P.Diederich Musée national d’histoire naturelle 25 rue MunsterL-2160 LuxembourgLuxembourg

Lichenicolous fungi belonging to the anamorphtypified genus Phaeosporobolus and to the teleomorph-typified genus Lichenostigma were isolated in pure culture or sequenced directly,with nuLSU and mtSSU sequences *** analyses place the species of Phaeosporobolusin a strongly supported clade with the generic type of Lichenostigma(***),the genus Phaeococcomyces and several melanized rock-inhabiting *** strongly supported nonlichenized lineage is sister to the primarily lichenized Arthoniales in the Arthoniomycetes and is here described as the *** new order is characterized by cells multiplying by budding,either representing black yeasts,or species in which conidiomata and ascomata are entirely made of an organised agglomeration of spherical yeast-like *** way of life is not only very different from all other Arthoniomycetes that exist only in the mycelial stage,but ascomata and conidiomata representing a dense and organised agglomeration of yeast cells might be unique amongst fungi.A further difference with the Arthoniales is the absence of *** results suggest that Phaeosporobolus usneae is the asexual stage of Lichenostigma *** species of Phaeosporobolus are transferred to the genus Lichenostigma except ***,for which the new genus Etayoa is *** genus Diederimyces is reduced into synonymy with *** other members of Lichenostigma are placed in the Dothideomycetes and are intermixed with Lichenothelia species.

关键词： Anamorphic fungi Arthoniomycetes Dothideomycetes Lichenostigmatales Phaeococcomycetaceae Yeasts

Molecular data place the hyphomycetous lichenicolous genus Sclerococcum close to Dactylospora (Eurotiomycetes) and S. parmeliae in Cladophialophora (Chaetothyriales)

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Fungal Diversity 2013年第1期58卷 61-72页

作者： paul Diederich damien Ertz James D.Lawrey Masoumeh Sikaroodi Wendy A.Untereiner Musée national d’histoire naturelle 25 rue MunsterL-2160LuxembourgLuxembourg Jardin botanique national de Belgique Département bryophytes-thallophytesDomaine de BouchoutB-1860MeiseBelgium Department of Environmental Science and Policy George Mason University4400 University DriveFairfaxVA 22030-4444USA Department of Biology Brandon UniversityBrandonMB R7A 6A9Canada

The lichenicolous anamorphic fungus Sclerococcum parmeliae was isolated in pure culture,and ITS,nuLSU and mtSSU sequences were obtained from these *** comparison,sequences from ***,the generic type,were obtained directly from freshly collected *** analyses place *** with species of Dactylospora and an unidentified lichen-inhabiting isolate in a strongly supported clade that is sister to a lineage comprising members of the Chaetothyriales and *** contrast,*** is inferred as a member of the Herpotrichiellaceae(Chaetothyriales)and belongs to a robustly supported clade that also includes species of Cladophialophora,Capronia semiimmersa,and Phialophora *** the Herpotrichiellaceae,*** most closely resembles members of the anamorph genus ***,we propose the transfer of *** and the morphologically similar species ***,*** and *** to Cladophialophora.A new lichenicolous species,***,collected twice on Megalospora in Florida and Papua New Guinea,is also described.

关键词： Anamorphic fungi Capronia Conidial fungi Hyphomycetes Parasitic fungi

Briancoppinsia,a new coelomycetous genus of Arthoniaceae(Arthoniales)for the lichenicolous Phoma cytospora,with a key to this and similar taxa

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Fungal Diversity 2012年第1期52卷 1-12页

作者： paul Diederich James D.Lawrey Masoumeh Sikaroodi Pieter P.Gvan den Boom damien Ertz Musée national d’histoire naturelle 25 rue Munster2160 LuxembourgLuxembourg Department of Environmental Science and Policy George Mason University4400 University DriveFairfaxVA 22030-4444USA Arafura 16 5691 JA Sonthe Netherlands Jardin Botanique National de Belgique Domaine de Bouchout1860 MeiseBelgium

Morphological,anatomical,chemical and molecular data suggest that a relatively common lichenicolous coelomycete on Lecanora conizaeoides is conspecific with Phoma cytospora,previously known only from parmelioid lichens,and that further populations on Cladonia and Pertusaria belong to the same *** species is distinguished from Phoma by several taxonomically important characters and obviously represents a previously unrecognized genus,for which the name Briancoppinsia is *** analyses using nuLSU and mtSSU sequences of isolates obtained in pure culture suggest that the new genus belongs to the Arthoniaceae(Arthoniales).This is the first obligate lichenicolous,non-lichenized anamorph confirmed to belong to the Arthoniales based on molecular data.

关键词： Anamorphic fungi Arthonia Conidial fungi Mitosporic fungi

Phylogenetic insights resolve Dacampiaceae(Pleosporales)as polyphyletic:Didymocyrtis(Pleosporales,Phaeosphaeriaceae)with Phoma-like anamorphs resurrected and segregated from Polycoccum(Trypetheliales,Polycoccaceae ***.)

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Fungal Diversity 2015年第5期74卷 53-89页

作者： damien Ertz paul Diederich James D.Lawrey Franz Berger Colin E.Freebury Brian Coppins Alain Gardiennet Josef Hafellner Department Bryophytes-Thallophytes(BT) Botanic Garden MeiseNieuwelaan 38B-1860 MeiseBelgium Musée national d’histoire naturelle 25 rue MunsterL-2160 LuxembourgLuxembourg Department of Biology George Mason University4400 University DriveFairfaxVA 22030USA Raiffeisenweg 130 A-4794 KopfingAustria Canadian Museum of Nature PO Box 3443Stn.BDOttawaOntarion K1P 6P4Canada Royal Botanic Garden Edinburgh 20A Inverleith RowEdinburgh EH35LRUK 14 rue Roulette F-21260 VéronnesFrance Institute for Plant Sciences NAWI GrazKarl-Franzens-University GrazHolteigasse 6A-8010 GrazAustria

A phylogenetic analysis of nuLSU and ITS sequences representing genera previously included in Dacampiaceae indicates that the family is strongly polyphyletic and that the type species of Dacampia is placed in *** genus Munkovalsaria *** placed in Didymosphaeriaceae(Pleosporales).Polycoccum *** two species of Clypeococcum are shown to form a new lineage sister to the Trypetheliaceae in Trypetheliales and described here as *** members of Polycoccum *** included in the Pleosporales and are closely related to lichenicolous Phoma-like species of the family *** genus Didymocyrtis is resurrected for these species and for lichenicolous species previously assigned to Diederichia,Diederichomyces,Leptosphaeria and *** genera Diederichia and Diederichomyces are synonymized with *** new combinations Didymocyrtis bryonthae,***,***,***,***,***,***,***,*** and *** are made,and the new name *** is introduced for Phoma *** anamorph-teleomorph relationships are resolved,such as Didymocyrtis ramalinae–Phoma ficuzzae and Didymocyrtis consimilis–Phoma caloplacae,the phylogenetic results being supported by single ascospore cultures that lead to the asexual stage producing pycnidia and conidia in *** by host switching is assumed to be important in the genus *** identification key to Didymocyrtis species is provided.

关键词： Dothideomycetes Leptosphaeria Diederichia Diederichomyces Lichenicolous fungi Phylogeny

Phylogenetic placement of lichenicolous Phoma species in the Phaeosphaeriaceae(Pleosporales,Dothideomycetes)

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Fungal Diversity 2012年第4期55卷 195-213页

作者： James D.Lawrey paul Diederich Matthew P.Nelsen Colin Freebury Dries Van den Broeck Masoumeh Sikaroodi damien Ertz Department of Environmental Science and Policy George Mason University4400 University DriveFairfaxVA 22030-4444USA Musée national d’histoire naturelle 25 rue MunsterL-2160 LuxembourgLuxembourg Committee on Evolutionary Biology University of Chicago1025 E.57th StreetChicagoIL 60637USA Department of Botany The Field Museum1400 S.Lake Shore DriveChicagoIL 60605USA Canadian Museum of Nature P.O.Box 3443Station DOttawaON K1P 6P4Canada Jardin Botanique National de Belgique Domaine de BouchoutB–1860 MeiseBelgium

More than twenty species of lichenicolous fungi have been described in Phoma,a large anamorphic genus of primarily plant-associated pathogens with broad geographic *** obtained nuclear and mitochondrial rDNA sequences from 19 fungal cultures isolated from specimens representing four described and two undescribed lichenicolous species in the *** multilocus phylogeny indicates that lichenicolous Phoma species represent at least two phylogenetically distinct clades in the Phaeosphaeriaceae,one including a new species,Phoma puncteliae,isolated from a specimen of Punctelia rudecta collected inMaryland,USA,and another group of primarily lichenicolous *** latter group includes four described lichenicolous Phoma species,an unidentified melanized rock fungus,and a new lichenicolous Phoma species isolated from Xanthomendoza species collected in Canada that we are naming *** specimens in this clade collected from different lichen genera and species were found to be very similar genetically,which calls into question the recent practice of recognizing lichenicolous Phoma species mainly by differences in host preference.

关键词： Anamorphic fungi Coelomycetes Conidial fungi Parasitic fungi

Tetanus in a post operative patient—A case report

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Case Reports in Clinical Medicine 2013年第1期2卷 32-34页

作者： paul Dienye Andrew Bock-Oruma damien Uyagu Department of Family Medicine University of Benin Teaching Hospital Benin City Nigeria Department of Family Medicine University of Port Harcourt Teaching Hospital Port Harcourt Nigeria

Tetanus is a preventable disease which commonly occurs with puncture or penetrating wounds, or contamination of cutaneous wounds. It may be secondary to surgical procedures such as gastrointestinal surgery on rare occasions. This article reports a fatal case of tetanus in a post operative patient in which the source of infection could not be ascertained. Active immunization of all persons against tetanus, adequate sterilization of surgical equipment and consumables as well as the operating rooms is advocated.

关键词： Appendectomy Post Operative Tetanus Immunisation Developing Countries

Prevalence and Associated Factors of Occupational Stress in the Mining Sector: The Case Study of the Youga Mine at Zabre in Burkina Faso

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Occupational Diseases and Environmental Medicine 2021年第4期9卷 185-198页

作者： Antoine V. Hinson Badirou Aguemon Menonli Adjobimey Georgia damien Ibrahime Ouili Rose Mikponhoue Ibrahim Mama Cisse Fabien Gounongbe paul Ayelo Unit of Teaching and Research in Occupational and Environmental Health Faculty of Health Sciences University of Abomey-Calavi Benin Unit of Teaching and Research in Public Health Faculty of Health Sciences University of Abomey-Calavi Benin Faculty of Medicine University of Parakou Benin

Introduction: Occupational stress (OS) is a public health issue;it affects all sectors of activity in Burkina Faso. General objective: Assess the situation of professional stress and its associated factors among workers at the Youga mine in the municipality of Zabré. Material and Methods: This was a descriptive and analytical cross-sectional study. Data collection took place August 15, 2020 to October 10, 2020 from 324 mine workers who met the inclusion criteria using an anonymous questionnaire. Data analysis was done with EpiDATA3.1 software. The Fisher and Chi-squared tests were used to compare the proportions. A binary logistic regression was performed by the stepwise top-down method. The significance level chosen for the tests was p ≤ 0.05. Results: In our study, 324 workers were included. The average age was 35.84 ± 6.56 years with extremes of 23 years and 58 years. The sex ratio was 8. The prevalence of OS was 26.23% and that of iso strain and lack of rewards were 24.38% and 45.06%, respectively. Factors associated with OS were marital status p = 0.010;higher education level p = 0.032, lack of recognition p = 0.001, intention to change jobs p = 0.009, job security p = 0.035;job satisfaction p = 0.046 and the stress felt by the worker at his job p = 0.003. Conclusion: Occupational stress is a reality in the mining sector in Burkina Faso, our study consisted of evaluating the extent and determining the factors of OS in order to propose preventive measures for the good health of workers.

关键词： Occupational stress Prevalence Mine Zabré Burkina Faso