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Lineage specification of early embryos and embryonic stem cells at the dawn of enabling technologies

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National Science Review 2017年第4期4卷 533-542页

作者： Guangdun Peng patrick P.L.tam Naihe Jing State key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of SciencesUniversity of Chinese Academy of Sciences Embryology Unit Children’s Medical Research Institute School of Medical SciencesSydney Medical School University of Sydney School of Life Science and Technology Shanghai Tech University

Establishment of progenitor cell populations and lineage diversity during embryogenesis and the differentiation of pluripotent stem cells is a fascinating and intricate biological process. Conceptually, an understanding of this developmental process provides a framework to integrate stem-cell pluripotency, cell competence and differentiating potential with the activity of extrinsic and intrinsic molecular *** recent advent of enabling technologies of high-resolution transcriptome analysis at the cellular,population and spatial levels proffers the capability of gaining deeper insights into the attributes of the gene regulatory network and molecular signaling in lineage specification and differentiation. In this review, we provide a snapshot of the emerging enabling genomic technologies that contribute to the study of development and stem-cell biology.

关键词： early development cell lineage cell identity scRNA-seq spatial transcriptome

A Physicist's Guide to Mathematica, Second Edition 2

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Integration of Computational Analysis and Spatial Transcriptomics in Single-cell Studies

2008年

作者： patrick T. tam

来源：畅想之星图书光盘版（南... 评论

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Genomics, Proteomics & Bioinformatics 2023年第1期21卷 13-23页

作者： Ran Wang Guangdun Peng patrick P.L.tam Naihe Jing State key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China CAS key Laboratory of Regenerative Biology Guangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China Embryology Research Unit Children’s Medical Research InstituteUniversity of SydneySydneyNSW 2145Australia School of Medical Sciences Faculty of Medicine and HealthUniversity of SydneySydneyNSW 2145Australia Guangzhou Laboratory Guangzhou 510005China

Recent advances of single-cell transcriptomics technologies and allied computational methodologies have revolutionized molecular cell ***,pioneering explorations in spatial transcriptomics have opened up avenues to address fundamental biological questions in health and ***,we review the technical attributes of single-cell RNA sequencing and spatial transcriptomics,and the core concepts of computational data *** further highlight the challenges in the application of data integration methodologies and the interpretation of the biological context of the findings.

关键词： scRNA-seq Computational methodology Spatial transcriptome Data integration Mathematical model

Defining cell identity beyond the premise of differential gene expression

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Cell Regeneration 2021年第1期10卷 226-228页

作者： hani Jieun kim patrick P.L.tam Pengyi Yang School of Mathematics and Statistics The University of SydneySydneyNSW 2006Australia Computational Systems Biology Group Children’s Medical Research InstituteFaculty of Medicine and HealthThe University of SydneyWestmeadNSW 2145Australia Charles Perkins Centre The University of SydneySydneyNSW 2006Australia Embryology Unit Children’s Medical Research InstituteFaculty of Medicine and HealthThe University of SydneyWestmeadNSW 2145Australia School of Medical Science Faculty of Faculty of Medicine and HealthThe University of SydneySydneyNSW 2006Australia

Identifying genes that define cell identity is a requisite step for characterising cell types and cell states and predicting cell fate *** far,the most widely used approach for this task is based on differential expression(DE)of genes,whereby the shift of mean expression are used as the primary statistics for identifying gene transcripts that are specific to cell types and *** DE-based methods are useful for pinpointing genes that discriminate cell types,their reliance on measuring difference in mean expression may not reflect the biological attributes of cell identity ***,we highlight the quest for non-DE methods and provide an overview of these methods and their applications to identify genes that define cell identity and functionality.

关键词： beyond cell identity

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学术短评:两项关于胆道闭锁的实践与技术

临床小儿外科杂志 2020年第9期19卷 856-857页

作者： patrick Chung Paul tam 邓喜成(翻译) 香港大学李嘉诚医学院玛丽医院外科香港特别行政区810000 湖南省儿童医院湖南省长沙市410007

2019年第12期的《Journal of Pediatric Surgery》刊登了两项来自日本的相关胆道闭锁诊治的临床研究[1,2]。其中一项主题是“肝穿刺活检有可能延迟葛西肝门肠吻合术的实施,在腹腔镜技术时代用其诊断胆道闭锁已经过时”;另一项的主题是... 详细信息

2019年第12期的《Journal of Pediatric Surgery》刊登了两项来自日本的相关胆道闭锁诊治的临床研究[1,2]。其中一项主题是“肝穿刺活检有可能延迟葛西肝门肠吻合术的实施,在腹腔镜技术时代用其诊断胆道闭锁已经过时”;另一项的主题是“胆道闭锁手术中使用近红外荧光胆管造影结合吲哚菁绿实时评估胆道流量的结果”。

关键词：胆道闭锁吲哚菁绿肝穿刺活检腹腔镜技术胆管造影 Surgery 肝门肠吻合术近红外荧光

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Portage of Bacteria Responsible of Foodborne Illness in Scholarly Canteens (Republic of Benin)

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Advances in Microbiology 2012年第3期2卷 340-344页

作者： honoré Sourou Bankolé Tossou Jacques Dougnon patrick Aléodjrodo Edorh tamègnon Victorien Dougnon Magloire Legonou Jean Robert klotoé Frédéric Loko Interfaculty Center of Formation and Research in Environment for the Sustainable Development Laboratory of Toxicology and Environmental Health University of Abomey-Calavi (UAC) Cotonou Benin Laboratory of Food Microbiology Ministry of Health Cotonou Benin Polytechnic School of Abomey-Calavi Research Laboratory in Applied Biology University of Abomey-Calavi Cotonou Benin

This study has determined the portage of bacteria responsible for foodborne illness in the school canteen staff. 336 samples taken on the nose, mouth and hands were collected. Microbiological analyses were realized and several pathogenic bacterial strains were isolated from the vendors: Staphylococcus aureus (26/122), sulphite-reducing clostridia (14/122) and Escherichia coli (10/122). The food vendors in the school canteen may be the vectors of germs that cause food poisoning among young students.

关键词： Portage Bacteria Food Escherichia coli Disease

Phytochemical Screening, Nutritional and Toxicological Analyses of Leaves and Fruits of <i>Solanum macrocarpon</i>Linn (Solanaceae) in Cotonou (Benin)

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Food and Nutrition Sciences 2012年第11期3卷 1595-1603页

作者： tamègnon Victorien Dougnon honoré Sourou Bankolé Roch Christian Johnson Jean Robert klotoé Godfried Dougnon Fernand Gbaguidi Fidèle Assogba Joachim Gbénou Salifou Sahidou Jean-Marc Atègbo Bertrand henri Rhin Frédéric Loko Michel Boko Aléodjrodo patrick Edorh Department of Animal Physiology Faculty of Science and Technology University of Abomey-Calavi (UAC) Cotonou Benin Laboratory of Pharmacognosy and Essentials Oils Institute of Applied Biomedical Sciences Champ de Foire Cotonou Benin Laboratory of Toxicology and Environmental health Interfaculty Center of Formation and Research in Environment for the Sustainable Development University of Abomey-Calavi (UAC) Cotonou Benin National Laboratory of Pharmacognosy Benin Center of Scientific and Technical Research and Essential Oils Porto-Novo Benin Research Laboratory in Applied Biology Polytechnic School of Abomey-Calavi University of Abomey-Calavi Cotonou Benin Unit Inserm U525 Faculty of Pharmacy Nancy France Unit of Training and Research in Pharmacy Faculty of Health Sciences Champ de Foire Cotonou Benin

Vegetables are very important sources of protein and minerals. Some of them even have medicinal properties recognized traditionally. Despite the large number of studies carried out on various vegetables and vegetable crops, very few have scientifically explored the usefulness of S. macrocarpon. This study identified the main groups of chemicals and mineral elements to explain any medicinal or nutritional value. It has also identified some toxic elements contained in this vegetable. Phytochemical screening was carried out on the leaves and fruits of S. macrocarpon. Some mineral elements were determined by Atomic Absorption Spectrophotometry (sodium, potassium, calcium, magnesium) while protein, phosphorous, iron, copper, zinc and toxic metals (lead, cadmium) were determined by Molecular Absorption Spectrophotometry. Fat, ash, moisture and vitamins were sought. The study showed that the leaves of S. macrocarpon were more nutritious than fruits (P macrocarpon require that the consumption of vegetables should be as varied as possible and that the fruit may be consumed with caution. In addition, constraints about vegetable cultivation in Cotonou could lead to research findings that could help provide techniques for producing healthy *** A and k1 were found in both parts of this vegetable very rich in water while vitamin E has not been detected. S. macrocarpon also contains lipids at various levels.

关键词： S. macrocarpon Nutrients Minerals Lead Cadmium Cotonou

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北京侨福芳草地

城市环境设计 2013年第8期 232-237页

作者：徐腾 Ulrich Blum Nicole Ip Alicia Tan Pusey Chan Stephane Lacroix Moses Tan kin Choy Christa Lange Jochen Tombers howard Chung Peter Liu Louisa Williams Colin Foster Rosita Nicknafs patrick Yue Simon Griffiths Ed Peter Connie ho William tam IDA建筑设计事务所

2000年岁末,北京市中心,来自香港的侨福集团为一座地标性建筑提出了一项挑战性的策划,旨在改变大型综合商业开发项目的成规。它所引得的回应,也是一项时尚、节能且环保的设计,由徐腾(Winston Shu)领衔的香港建筑事务所IDA一手命制。整... 详细信息

2000年岁末,北京市中心,来自香港的侨福集团为一座地标性建筑提出了一项挑战性的策划,旨在改变大型综合商业开发项目的成规。它所引得的回应,也是一项时尚、节能且环保的设计,由徐腾(Winston Shu)领衔的香港建筑事务所IDA一手命制。整个开发项目有微气候调节,运用与北京季候变化相一致的可持续自然手段,实现了水准空前的热舒适度与灵活的使用空间。项目由四座建筑组成,位于一座下沉庭园之中,低于毗邻街道地平面9m。所有的建筑彼此独立,中间是一个L形的满光中庭,宽达24m;其中有空中花园、露台、廊桥彼此穿插错落,均在一道

关键词：精品酒店廊桥商业微气候微小气候办公楼零售商场绿色建筑绿地集团 LEED 北京庭园园林中庭空中花园露台园林建筑设计团队

原肠运动中期细胞谱系与细胞命运的空间转录组特征

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科学新闻 2017年第4期 106-106页

作者： Guangdun Peng Shengbao Suo Jun Chen Weiyang Chen Chang Liu Fang Yu Ran Wang Shirui Chen Na Sun Guizhong Cui Lu Song patrick P.L.tam 韩敬东景乃禾中国科学院上海生命科学研究院计算生物学研究所中科院计算生物学重点实验室中国科学院上海生命科学研究院生物化学与细胞生物学研究所中国科学院细胞生物学国家重点实验室

文章简介哺乳动物的早期胚胎发育,特别是着床后原肠运动过程中,多能性干细胞通过多个层次的细胞命运决定,形成了胚胎器官发生、形态建成的整个发育蓝图,是生命体最重要的分子事件之一。在此过程中,不同细胞在胚胎中的空间位置决定了其... 详细信息

文章简介哺乳动物的早期胚胎发育,特别是着床后原肠运动过程中,多能性干细胞通过多个层次的细胞命运决定,形成了胚胎器官发生、形态建成的整个发育蓝图,是生命体最重要的分子事件之一。在此过程中,不同细胞在胚胎中的空间位置决定了其未来的分化潜能和发育方向。因此,对早期胚胎进行空间位置特异的转录组分析,可以解析早期

关键词：细胞命运肠运动上胚层外胚层转录组