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Identity and distribution of weedy Pedicularis kansuensis Maxim. (Orobanchaceae) in Tianshan Mountains of Xinjiang：morphological, anatomical and molecular evidence

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Journal of Arid Land 2016年第3期8卷 453-461页

作者： SUI Xiaolin patrick kuss LI Wenjun YANG Meiqing GUAN Kaiyun LI Airong Yunnan Key Laboratory for Research and Development of Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Kunming 650201 China Institute of Systematic Botany University of Zurich Zurich 8008 Switzerland Key Laboratory of Biogeography and Bioresource in Arid Land Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences Urumqi 830011 China University of Chinese Academy of Sciences Beijing 100049 China World Agroforestry Centre East and Central Asia Region Kunming 650201 China

Weedy plants affect the biodiversity and ecosystem function as well as the crop and fodder plant production. However, adequate management requires detailed knowledge of the taxonomic identity of these plants. Here, we focused on a hemiparasitic Pedicularis species（Orobanchaceae）, which occurs at high densities and results in significant biomass reductions in forage grasses in Bayanbulak Grassland of Xinjiang. The identity of this target species is not clear, with conflicting reports in publications and in herbarium collections. Hence, clear and management-relevant information on demography and reproductive ecology is difficult to be obtained from the literature. Therefore, we analyzed field and archival materials collected from Xinjiang in order to clarify the identity and distribution of the target species. Morphological analyses suggested that the populations at Bayanbulak Grassland should be Pedicularis kansuensis Maxim. rather than P. verticillata L. which has been accepted in the available literature. Phylogenetic analysis with a combination of three barcodes（mat K, rbc L and trn H-psb A） uniting a clade of P. kansuensis and individuals from Bayanbulak Grassland populations with 100% bootstrap support, confirmed the target species to be P. kansuensis. Anatomical investigations and field observations showed that the target species is an annual or biennial herb, which also fits with the life cycle as P. kansuensis. Based on archive material and field observations, we verified that the distribution of P. kansuensis is mainly concentrated in the Tianshan Mountains of Xinjiang.

关键词： DNA barcode herb-chronology morphological characters root hemiparasite

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Spatial isolation and genetic differentiation in naturally fragmented plant populations of the Swiss Alps

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Journal of Plant Ecology 2008年第3期1卷 149-159页

作者： patrick kuss AndreaRPluess Hafdı´s HannaÆgisdottir Jurg Stocklin Institute of Botany University of BaselSchonbeinstrasse 6CH-4056BaselSwitzerland Institute of Plant Sciences University of BernAltenbergrain21CH-3013BernSwitzerland Institute for Terrestrial Ecosystems Ecosystem ManagementETH ZurichUniversitatstrasse 168092 ZurichSwitzerland

The effect of anthropogenic landscape fragmentation on the genetic diversity and adaptive potential of plant populations is a major issue in conservation biology.However,little is known about the partitioning of genetic diversity in alpine species,which occur in naturally fragmented habitats.Here,we investigate molecular patterns of three alpine plants(Epilobium fleischeri,Geum reptans and Campanula thyrsoides)across Switzerland and ask whether spatial isolation has led to high levels of population differentiation,increasing over distance,and a decrease of within-population variability.We further hypothesize that the contrasting potential for long-distance dispersal(LDD)of seed in these species will considerably influence and explain diversity partitioning.Methods For each study species,we sampled 20–23 individuals from each of 20–32 populations across entire Switzerland.We applied Random Amplified Polymorphic Dimorphism markers to assess genetic diversity within(Nei’s expected heterozygosity,He;percentage of polymorphic bands,Pp)and among(analysis of molecular variance,Ust)populations and correlated population size and altitude with within-population diversity.Spatial patterns of genetic relatedness were investigated using Mantel tests and standardized major axis regression as well as unweighted pair group method with arithmetic mean cluster analyses and Monmonier’s algorithm.To avoid known biases,we standardized the numbers of populations,individuals and markers using multiple random reductions.We modelled LDD with a high alpine wind data set using the terminal velocity and height of seed release as key parameters.Additionally,we assessed a number of important life-history traits and factors that potentially influence genetic diversity partitioning(e.***.breeding system,longevity and population size).Important findings For all three species,we found a significant isolation-by-distance relationship but only a moderately high differentiation among populations(Ust:22.7,14.8 and 16.8%,for E.fleischeri,G.reptans and C

关键词： Campanula thyrsoides Epilobium fleischeri Geum reptans isolation by distance molecular diversity

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3D printing of silk fibroin-based hybrid scaffold treated with platelet rich plasma for bone tissue engineering

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Bioactive Materials 2019年第1期4卷 256-260页

作者： Liang Wei Shaohua Wu Mitchell kuss Xiping Jiang Runjun Sun Reid patrick Xiaohong Qin Duan Bin School of Textile Science and Engineering Xi'an Polytechnic UniversityXi'an710048PR China Mary&Dick Holland Regenerative Medicine Program Division of CardiologyDepartment of Internal MedicineUniversity of Nebraska Medical CenterOmahaNE68198USA Key Laboratory of Textile Science&Technology Ministry of EducationCollege of TextilesDonghua UniversityShanghai201620PR China College of Textiles&Clothing Qingdao UniversityQingdao266071PR China Department of Pathology&Microbiology College of MedicineUniversity of Nebraska Medical CenterOmahaNE68198USA Department of Surgery College of MedicineUniversity of Nebraska Medical CenterOmahaNE68198USA Department of Mechanical and Materials Engineering University of Nebraska-LincolnLincolnNE68516USA

3D printing/bioprinting are promising techniques to fabricate scaffolds with well controlled and patient-specific structures and architectures for bone tissue engineering.In this study,we developed a composite bioink consisting of silk fibroin(SF),gelatin(GEL),hyaluronic acid(HA),and tricalcium phosphate(TCP)and 3D bioprinted the silk fibroin-based hybrid scaffolds.The 3D bioprinted scaffolds with dual crosslinking were further treated with human platelet-rich plasma(PRP)to generate PRP coated scaffolds.Live/Dead and MTT assays demonstrated that PRP treatment could obviously promote the cell growth and proliferation of human adipose derived mesenchymal stem cells(HADMSC).In addition,the treatment of PRP did not significantly affect alkaline phosphatase(ALP)activity and expression,but significantly upregulated the gene expression levels of late osteogenic markers.This study demonstrated that the 3D printing of silk fibroin-based hybrid scaffolds,in combination with PRP post-treatment,might be a more efficient strategy to promote osteogenic differentiation of adult stem cells and has significant potential to be used for bone tissue engineering.

关键词： 3D bioprinting Hybrid scaffold Coating Growth factor cocktail Tissue engineering

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Laparoscopic Transperitoneal Pyeloplasty for Ureteropelvic Junction Obstruction: Preliminary Results from 26 Cases

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Surgical Science 2023年第5期14卷 309-320页

作者： Cyril Kamadjou Divine Enoru Eyongeta Annie Kameni Wadeu patrick Fotso Gwabap Bertin Njinou Ngninkeu Edouard Hervé Moby Medico Surgical Centre of Urology and Mini Invasive Surgery Douala Cameroon Department of Surgery and Specialties Faculty of Medicine and Pharmaceutical Sciences University of Douala Douala Cameroon Department of Surgery Faculty of Health and Sciences University of Buea Buea Cameroon Clinic of Aeroport Douala Cameroon

Background and Objectives: Pyelo-Ureteral Junction Syndrome (PUJS) is the most common obstructive malformation of the upper urinary tract in children and adults. The standard treatment is open pyeloplasty using the kuss-Anderson technique. Because of the minimally invasive nature of the laparoscopic approach, it has become the approach of choice for the management of this pathology. We present the results of laparoscopic transperitoneal pyeloplasty used in the management of PUJS. Materials and Methods: This was a retrospective study from 2015 to 2020, including 26 patients who underwent laparoscopic transperitoneal pyeloplasty for PUJS. Results: Twenty-six patients, including 18 (69.2%) men and 6 (30.8%) women, with a mean age of 35.42 ± 13.62 years, were treated. Renal colic was the primary symptom in the majority of the cases (22, 84.6%)). The diagnosis was confirmed in all patients through an abdominal CT scan, which revealed that all 26 patients had hydronephrosis. Half of the patients (50.0%) were classified as Valayer-Cendron Type II, and eight (30.8%) patients had associated stones. Seventeen (65.4%) patients had pathologies on the left side, and all patients were treated using the kuss-Anderson technique with a median operating time of 108.5 (90.0 - 136.0) minutes. The uncrossing of lower pole vessels was performed in 10 patients. The average duration of hospitalisation was 2.23 ± 0.82 days. No conversion was observed, and after an average follow-up of 53.69 days, the success rate was 92.3%. Conclusion: Laparoscopic pyeloplasty is a minimally invasive technique of choice for the treatment of pyelo-ureteral junction obstruction. It is dependable, repeatable, and produces good functional outcomes that are equal to those of traditional surgery.

关键词： Pyelo-Ureteral Junction Lower Pole Vessel Pyeloplasty Double J Stent

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