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Plectranthus amboinicus(Lour.)*** oil as a natural alternative for the conservation of beef patties stored under refrigeration

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Food Bioscience 2022年第5期49卷 397-405页

作者： Bruno Dutra da Silva patricia Campos Bernardes patrícia fontes pinheiro Juliana Di Giorgio Giannotti Consuelo Domenici Roberto Department of Food Engineering Center for Agrarian Sciences and EngineeringUniversidade Federal do Espírito SantoAlegreEspírito SantoBrazil Department of Chemistry and Physics Center for ExactNatural and Health SciencesUniversidade Federal do Espírito SantoAlegreEspírito SantoBrazil Department of Rural Engineering Center for ExactNatural and Health SciencesUniversidade Federal do Espírito SantoAlegreEspírito SantoBrazil

Plectranthus amboinicus essential oil(PAEO)has the potential to be used by the food industry as a natural antimicrobial *** study aimed to evaluate the antibacterial activity of PAEO in vitro against pathogenic bacteria contaminating food and its potential for action when added to intentionally contaminated beef *** composition was determined by GC-MS and GC-FID,and thymol(45.64%)was the main compound present in the *** minimum inhibitory(MIC)and minimum bactericidal(MBC)concentration of the PAEO was evaluated against five pathogenic bacteria(Staphylococcus aureus,Listeria monocytogenes,Escherichia coli,Pseudomonas aeruginosa and Salmonella Typhimurium).The MIC of PAEO was the concentration ranges between 0.25 and 2 mg mL^(-1),and the MBC ranged from 1 to 4 mg mL^(-1).The anti-adhesion and removal of adhered cell assays indicated significant reductions(P<0.05)even at concentrations 8x lower than the MIC of *** patties samples were prepared with PAEO concentrations at MIC(2 mg mL^(-1))and sub-MIC(1 mg mL^(-1)).*** and *** were inoculated individually into the beef *** reductions were observed with a greater reduction in the 12th day of storage for ***(1.52 log CFU.g^(-1))and ***(0.93 log CFU.g^(-1))for beef patties with the MIC of *** has the potential to be applied in meat ***,the application and interactions of the oil in the food matrix need to be better elucidated.

关键词： Thymol Natural antimicrobial Anti-adhesion activity Minimum inhibitory concentration Antimicrobial activity

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Influence of Environmental and Microclimate Factors on the Coffee Beans Quality (C. canephora): Correlation between Chemical Analysis and Stable Free Radicals

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Agricultural Sciences 2018年第9期9卷 1173-1187页

作者： AndréLuíz Alves Marcio Solino Pessoa Paulo Eduardo Narcizo de Souza Fábio Luís Partelli Paulo Sérgio Moscon Edson Correa da Silva André Oliveira Guimaraes Eduardo Perini Muniz patrícia fontes pinheiro Flávio Meira Borém Paulo César Morais Departamento de Ciências Naturais Universidade Federal do Espírito SantoCentro Universitário Norte do Espírito SantoSao MateusBrazil Universidade de Brasília Instituto de FísicaNúcleo de Física AplicadaBrasília DFBrazil Laboratório de Ciências Físicas Centro de Ciências e TecnologiaUniversidade Estadual Norte FluminenseCampos dos GoytacasesBrazil Departamento de Química e Física Universidade Federal do Espírito SantoAlto UniversitárioAlegreBrazil Departamento de Engenharia Universidade Federal de LavrasLavrasBrazil School of Chemistry and Chemical Engineering Anhui UniversityHefeiChina

The present study reports a physicochemical comparison of shade-grown and sun-grown coffee beans, under unripe, rip and roasted-ripe conditions, using electrical conductivity measurements, electron paramagnetic resonance (EPR), infrared spectroscopy (FTIR), and high performance liquid chromatography (HPLC). Moreover, the assessed physicochemical parameters were compared with organoleptic evaluations based on the Coffee Quality Institute protocol. The values found for electrical conductivity, leached potassium, and stable free radicals were respectively 29%, 31%, and 350% higher for shade-grown coffee beans, whereas polyphenol oxidase enzymatic activity was 23% lower. By contrast, FTIR and HPLC measurements identified higher chlorogenic acid and lipid contents in sun-grown coffee beans. Importantly, the sensorial grade attributed to roasted-ripe grains was 12% higher for sun-grown coffee. Our findings suggest that shade-grown coffee beans have undergone microorganismal activity and undesired fermentation during cultivation, which resulted in lower coffee quality. A correlation between a set of selected physicochemical properties and organoleptic properties was robustly established and could be used in the development of future coffee bean quality control protocols.

关键词： Sun-Grown Coffee Shade-Grown Coffee Fermentation EPR FTIR HPLC

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Cardiovascular rehabilitation in patients aged 70-year-old or older:benefits on functional capacity,physical activity and metabolic profile in younger *** patients

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Journal of Geriatric Cardiology 2020年第9期17卷 544-553页

作者： Marta fontes-Oliveira Maria Trêpa patrícia Rodrigues Preza Fernandes Sandra Magalhães Sofia Cabral Mário Santos Severo Torres Cardiology Department University Hospital Centre of PortoPortoPortugal Cardiac Rehabilitation Unit Cardiology DepartmentUniversity Hospital Centre of PortoPortoPortugal Pulmonary Vascular Disease Unit University Hospital Centre of PortoPortoPortugal

Background The benefits of exercise-based cardiac rehabilitation(EBCR)programs in post-acute myocardial infarction(AMI)patients have been *** aim was to assess the impact of EBCR in³70-years-old *** post-AMI *** We retrospectively evaluated patients who underwent a supervised EBCR protocol,twice a week during 6-12 *** evaluated changes in several outcomes based on pre-and post-CRP *** Of a total of 1607 patients,333(21%)were³*** the EBCR,an overall improvement on functional capacity,daily physical activity,lipid profile,body mass index,glycated hemoglobin(HbA1c),N-terminal pro-brain natriuretic peptide(NT-pro-BNP)and C-reactive protein was observed in both younger and older patients(P<0.05).Older patients showed a smaller benefit on the increment of daily physical activity and lipid profile improvement,but a larger reduction in *** the multivariate analysis,only improvements on daily physical activity and HbA1c were dependent on *** As their younger counterparts,older patients,significantly improved functional capacity,metabolic parameters and level of daily physical activity after EBCR.

关键词： Aging Cardiovascular prevention Exercise-based cardiac rehabilitation

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Evolutionary fingerprint, phylogenetic and forest structure of tropical montane Atlantic cloud forests along an elevation gradient

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Journal of Mountain Science 2024年第4期21卷 1259-1271页

作者： Ravi Fernandes MARIANO Vanessa Leite REZENDE Cléber Rodrigo de SOUZA patrícia Vieira POMPEU Rubens Manoel dos SANTOS Carolina Njaime MENDES Aloysio Souza de MOURA Felipe Santana MACHADO Warley Augusto Caldas CARVALHO Marco Aurélio Leite fontes Department of Forest Sciences Federal University of LavrasLavras 37200-900Brazil 2Environmental Defence Association of Minas Gerais Belo Horizonte 30360-110Brazil Department of Ecology and Conservation Federal University of LavrasLavras 37200-900Brazil Department of Forest Sciences State University of Mato Grosso do SulAquidauana 79200-000Brazil Agência Zetta Federal University of LavrasLavras 37200-900Brazil. Department of Natural Sciences Federal University of São João del-ReiSão João del-Rei 36307-352Brazil.

Environmental conditions can change markedly over geographical distances along elevation gradients,making them natural laboratories to study the processes that structure *** work aimed to assess the influences of elevation on Tropical Montane Cloud Forest plant communities in the Brazilian Atlantic Forest,a historically neglected *** evaluated the phylogenetic structure,forest structure(tree basal area and tree density)and species richness along an elevation gradient,as well as the evolutionary fingerprints of elevation-success on phylogenetic lineages from the tree *** do so,we assessed nine communities along an elevation gradient from 1210 to 2310 m *** large elevation *** relationships between elevation and phylogenetic structure,forest structure and species richness were investigated through Linear *** occurrence of evolutionary fingerprint on phylogenetic lineages was investigated by quantifying the extent of phylogenetic signal of elevation-success using a genus-level molecular *** results showed decreased species richness at higher elevations and independence between forest structure,phylogenetic structure and *** also verified that there is a phylogenetic signal associated with elevation-success by *** concluded that the elevation is associated with species richness and the occurrence of phylogenetic lineages in the tree communities evaluated in Mantiqueira *** the other hand,elevation is not associated with forest structure or phylogenetic ***,closely related taxa tend to have their higher ecological success in similar ***,we highlight the fragility of the tropical montane cloud forests in the Mantiqueira Range in face of environmental changes(*** warming)due to the occurrence of exclusive phylogenetic lineages evolutionarily adapted to environmental conditions(*** temperature)associated with each elevation range.

关键词： Atlantic Forest Cloud forest Conservation Community ecology Montane forests Phylogenetic diversity Phylogenetic signal

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Hollow ion atomic structure and X-ray emission in dense hot plasmas

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Matter and Radiation at Extremes 2024年第6期9卷 43-52页

作者： Frank B.Rosmej Christopher J.fontes Sorbonne University Faculty of Science and EngineeringUMR 7605Case 1284 Place JussieuF-75252 ParisFrance Ecole Polytechnique LULIAtomic Physics in Dense PlasmasRoute de SaclayF-91129 PalaiseauFrance Computational Physics Division Los Alamos National LaboratoryLos AlamosNew Mexico 87545USA

Hollow ion X-ray emission is of great interest in high-energy-density research,since negligible opacity allows studies from the interior of very dense *** this paper,ionization potential depressions of the isoelectronic sequences for single and double K-shell vacancies are obtained from a pure ab initio multiconfiguration Hartree-Fock simulation including exact exchange terms and finite temperature dense plasma *** is demonstrated that the simultaneous representation of these ab initio data in the form of a map of hollow ion X-ray transition energies enables identification of important steps in the matter evolution and ionization *** along the isoelectronic sequence as a function of the pumping energy of a X-ray free electron laser also enables visualization of the impact of ionization potential depression on the pathways of hollow ion formation.

关键词： enable interior steps

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Comparative Proteomics Reveals Set of Oxidative Stress and Thaumatin-Like Proteins Associated with Resistance to Late Blight of Tomato

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American Journal of Plant Sciences 2018年第4期9卷 789-816页

作者： Bruno Soares Laurindo Renata Dias Freitas Laurindo patrícia Pereira fontes Camilo Elber Vital Fábio Teixeira Delazari Maria Cristina Baracat-Pereira Derly José Henriques da Silva Department of Phytotechny Federal University of Vi&ccedil osa Vi&ccedil osa Minas Gerais Brazil Department of Biochemistry and Molecular Biology Federal University of Vi&ccedil osa Vi&ccedil osa Minas Gerais Brazil Biomolecules Analysis Cente Federal University of Vi&ccedil osa Vi&ccedil osa Minas Gerais Brazil

Proteomics techniques were used to study the molecular mechanisms involved in the defense of tomato against late blight (Phytophthora infestans). Proteins were extracted from resistant access BGH-2127 and susceptible cultivar “Santa Clara”. Leaves of the inoculated and non-inoculated (control) genotypes were collected at 0, 2, and 48 h after inoculation and analyzed by two-dimensional electrophoresis (2-DE), followed by identification with mass spectrometry (MALDI TOF-TOF). A total of 56 differentially abundant proteins were identified, of which 39 were resistant genotypes and 17 were susceptible. These proteins were categorized into functional groups of energy and metabolism, photosynthesis, stress and defense, transcription, other proteins, and as un-characterized ones. For access BGH-2127, oxidative stress proteins (2-cis peroxiedoxin BAS1 and 2-cis peroxiredoxin) and thaumatin-like protein showed increase in the relative abundance at 0 and 48 h of inoculation, respectively, and were therefore considered important for the defense mechanism of this genotype. The expression standards evaluated by real-time PCR differed from the results of the proteomic analysis. The protein-protein interaction networks provided important information on the cellular activities involved in the resistance of BGH-2127 late blight.

关键词： Solanum lycopersicum L. Pathogenesis-Related (PR) Proteins Resistance to Phytophthora infestans

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Role of neuronal gap junctions in NMDA receptor-mediated excitotoxicity and ischemic neuronal death

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Neural Regeneration Research 2016年第1期11卷 75-76页

作者： Andrei B.Belousov Joseph D.fontes Department of Molecular and Integrative Physiology University of Kansas Medical Center Department of Biochemistry and Molecular Biology University of Kansas Medical Center

In the mammalian central nervous system（CNS）coupling of neurons by gap junctions and the expression of neuronal gap junction protein,connexin 36（Cx36）rapidly increases（usually during 1–2 hours）following a wide range of neuronal injuries,including ischemia,traumatic brain injury（TBI）,spinal cord injury and epilepsy（reviewed in Belousov and fontes,2013）.Pharmacological blockade or genetic elimi-nation of Cx36-containing gap junctions dramatically re- duce neuronal death in animal models of ischemia, TBI and epilepsy and prevent NMDA receptor （NMDAR）-mediated excitotoxicity （Belousov and fontes, 2014）.

关键词： neuronal epilepsy junction connexin traumatic NMDA injuries cortical mammalian minutes

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Method for grinding and delaminating muscovite

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Mining Science and Technology 2011年第1期21卷 7-10页

作者： Shirleny fontes Santos Silvia Cristina Alves Franca Tsuneharu Ogasawara Metallurgical and Materials Department Centre for Mineral Technology

A method of dry grinding muscovite based on the use of knife-mills is *** was possible to produce mica with a particle size below 100 and 45μ*** grinding,the samples were submitted to sonication treatment,which promoted delamination of the *** particle size distribution shows that sonicated mica has more particles in the size range 10-50μm than does non-sonicated mica have. This also indicates a decrease in the average particle *** of the treated muscovite by scanning electron microscopy revealed a highly delaminated material with a plate-like *** products were characterized by chemical analysis and X-ray diffraction,*** mica has already been used for the synthesis of pearlescent pigments.

关键词： Muscovite Grinding Ultrasound treatment Mineral processing

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Boron in Plants: Deficiency and Toxicity

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Journal of Integrative Plant Biology 2008年第10期50卷 1247-1255页

作者： Juan J. Camacho-Cristóbal Jesús Rexach Agustín Gonzlez-fontes Departamento de Fisiología Anatomía y Biología Celular Facultad de Ciencias Experimentales Universidad Pablo de OlavideE-41013 Sevilla Spain

Boron （B） is an essential nutrient for normal growth of higher plants, and B availability in soil and irrigation water is an important determinant of agricultural production. To date, a primordial function of B is undoubtedly its structural role in the ceil wall; however, there is increasing evidence for a possible role of B in other processes such as the maintenance of plasma membrane function and several metabolic pathways. In recent years, the knowledge of the molecular basis of B deficiency and toxicity responses in plants has advanced greatly. The aim of this review is to provide an update on recent findings related to these topics, which can contribute to a better understanding of the role of B in plants.

关键词： boron-binding molecules boron transporter cell wall-related genes gene expression nitrogen metabolism,

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Spectral classification of water masses under the influence of the Amazon River plume

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Acta Oceanologica Sinica 2010年第3期29卷 1-8页

作者： Gustavo Souto fontes Molleri Milton Kampel Evlyn Mrcia Leo de Moraes Novo National Institute for Space Research-INPE Remote Sensing Division So Jos dos Campos

The large amount of dissolved and particulate material discharged by the Amazon River into the Equatorial Atlantic Ocean cause distinct spectral response of its waters as compared to the nearby ocean waters. This paper shows the application of K-means clustering algorithm for classifying water masses in the region under the Amazon River plume influence according to their spectral behavior. Salinity and temperature data from 67 oceanographic stations were related to Sea-viewing Wide Field-of-view Sensor （SeaWiFS） remote sensing reflectances values and the following bio-optical products：（i） chlorophyll-a concentration, （ii） water attenuation coefficient and （iii） absorption coefficient for dissolved and detrital material. Four different water masses were identified such as：（1） oceanic water, （2） intermediate oceanic water, （3） intermediate river plume water and （4） Amazon River plume water. The spectral behavior of these water masses allowed concluding that the main active optical component of the waters in the region is the colored dissolved organic matter originated mostly from the Amazon River.

关键词： ocean color remote sensing amazon river plume spectral behavior cluster analysis

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