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3D imaging lipidometry in single cell by in-flow holographic tomography

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Opto-Electronic Advances 2023年第1期6卷 10-25页

作者： Daniele Pirone Daniele Sirico Lisa Miccio Vittorio Bianco Martina Mugnano Danila del Giudice Gianandrea Pasquinelli Sabrina Valente Silvia Lemma Luisa Iommarini Ivana Kurelac pasquale Memmolo Pietro Ferraro CNR-ISASI Institute of Applied Sciences and Intelligent Systems“E.Caianiello”Via Campi Flegrei 34PozzuoliNapoli 80078Italy Department of Electrical Engineering and Information Technologies University of Naples“FedericoⅡ”via Claudio 21Napoli 80125Italy Department of Chemical Materials and Production Engineering of the University of Naples FedericoⅡPiazzale Tecchio 80Napoli 80125Italy Department of Mathematics and Physics University of Campania“Luigi Vanvitelli”Caserta 81100Italy Biotechnology and Methods in Laboratory Medicine Department of ExperimentalDiagnostic and Specialty Medicine(DIMES)University of BolognaBologna 40126Italy Subcellular Nephro-Vascular Diagnostic Program Pathology Unit S.Orsola IRCCSUniversity of BolognaBologna 40126Italy Unit of Medical Genetics Department of Medical and Surgical Sciences(DIMEC)University of BolognaVia Massarenti 9Bologna 40138Italy Study and Research Center on Gynecological Neoplasias Department of Medical and Surgical Sciences(DIMEC)University of BolognaVia Massarenti 9Bologna 40138Italy Center for Applied Biomedical Research(CRBA) University of BolognaBologna 40138Italy Department of Pharmacy and Biotechnology(FABIT) University of BolognaBologna 40126Italy

The most recent discoveries in the biochemical field are highlighting the increasingly important role of lipid droplets(LDs)in several regulatory mechanisms in living *** are dynamic organelles and therefore their complete characterization in terms of number,size,spatial positioning and relative distribution in the cell volume can shed light on the roles played by *** now,fluorescence microscopy and transmission electron microscopy are assessed as the gold standard methods for identifying LDs due to their high sensitivity and ***,such methods generally only provide 2D assays and partial ***,both can be destructive and with low productivity,thus limiting analysis of large cell numbers in a *** we demonstrate for the first time the capability of 3D visualization and the full LD characterization in high-throughput with a tomographic phase-contrast flow-cytometer,by using ovarian cancer cells and monocyte cell lines as models.A strategy for retrieving significant parameters on spatial correlations and LD 3D positioning inside each cell volume is *** information gathered by this new method could allow more in depth understanding and lead to new discoveries on how LDs are correlated to cellular functions.

关键词： lipid droplets label-free phase-contrast imaging in-flow tomography 3D imaging

Trichoderma harzianum enhances tomato indirect defense against aphids

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Insect Science 2017年第6期24卷 1025-1033页

作者： Mariangela Coppola pasquale Cascone Maria Luisa Chiusano Chiara Colantuono Matteo Lorito Francesco Pennacchio Rosa Rao Sheridan Lois Woo Emilio Guerrieri Maria Cristina Digilio Department of Agricultural Sciences University of Naples Federico II Portici Italy CNR--IPSP Portici Italy

Many fungal root symbionts of the genus Trichoderma are well-known for their beneficial effects on agronomic performance and protection against plant pathogens; moreover, they may enhance protection from insect pests, by triggering plant resistance mechanisms. Defense barriers against insects are induced by the activation of metabolic pathways involved in the production of defense-related plant compounds, either directly active against herbivore insects, or exerting an indirect effect, by increasing the attrac- tion of herbivore natural enemies. In a model system composed of the tomato plant, the aphid Macrosiphum euphorbiae and the parasitoid Aphidius ervi, plant metabolic changes induced by Trichoderma harzianum and their effects on higher trophic levels have been assessed. T. harzianum T22 treatments induce a primed state that upon aphid attacks leads to an increased attraction of aphid parasitoids, mediated by the enhanced produc- tion of volatile organic compounds （VOCs） that are known to induce Aphidius ervi flight. Transcriptome sequencing of T22-treated plants infested by aphids showed a remarkable upregulation of genes involved in terpenoids biosynthesis and salicylic acid pathway, which are consistent with the observed flight response ofA. ervi and the VOC bouquet profile underlying this behavioral response.

关键词： defense gene defense priming Macrosiphum euphorbiae multitrophic interactions transcriptome VOCs

Internet as a Growing and Dynamic Network: An Economic View

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Communications and Network 2014年第2期6卷 69-75页

作者： pasquale Lucio Scandizzo Alessandra Imperiali University of Rome Tor Vergata Center for Economic and International Studies Rome Italy University of Rome Tor Vergata Rome Italy

The past few decades have witnessed renewed interest and research efforts on the part of the scientific community. After spending decades to disassemble nature, focusing the attention on its components, scientists have shifted their attention on complex networks. These basic structures constitute a wide range of systems in nature and society, but their design is irregular, evolves dynamically over time and their components can fit in a large multiplicity of alternative ways. Nevertheless, the most recent studies of networks have made remarkable progresses by investigating some critical issues of structure and dynamics, thereby improving the understanding of the topology and the growth processes of complex networks. From an economic point of view, networks are especially interesting because they can be considered as a problem of allocation of a critical resource, information, under multiple constraints. They can also be viewed as forms of poliarchies that reproduce, for many aspects, the market paradigm, with surprising properties of self-organization and resilience, which go much beyond the characteristics that are generally attributed to general equilibrium structures. In this paper we first address the major results achieved in the study of complex network and then focus our attention on two specific, highly dynamic and complex networks: Internet and the World Wide Web.

关键词： Network Analysis Internet Social Networks

Effectiveness of oral probiotics supplementation in the treatment of adult small chalazion

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International Journal of Ophthalmology(English edition) 2022年第1期15卷 40-44页

作者： Mariaelena Filippelli Roberto dell'Omo Angela Amoruso Ilaria Paiano Marco Pane pasquale Napolitano Giuseppe Campagna Silvia Bartollino Ciro Costagliola Department of Medicine and Health Sciences “V.Tiberio”University of MoliseCampobassoMolise 86100Italy Probiotical Research Srl R&D DepartmentNovaraPiemonte 28100Italy Department of Medical-Surgical Sciences and Translational Medicine University of Rome“Sapienza”Rome 00185Italy

AIM:To define the possible beneficial impact of probiotics oral supplementation on patients affected by ***:Prospective comparative pilot study on 20 adults suffering from chalazion randomly divided into two *** first group(n=10)received conservative treatment with lid hygiene,warm compression,and dexamethasone/tobramycin ointment for at least 20 *** second group(n=10),in addition to the conservative treatment,received a mixture of probiotic microorganisms of Streptococcus thermophilus ST10(DSM 25246),Lactococcus lactis LLC02(DSM 29536)and Lactobacillus delbrueckii(DSM 16606)once a day up to 3 *** were classified according to their size into three groups:small(<2 mm),medium(≥2 to<4 mm),or large(≥4 mm).When conservative treatment with and without probiotics supplementation failed to resolve the lesion,invasive methods were used,intralesional steroid injection in medium size chalazion and surgical incision and curettage for the largest ***:Medical treatment with or without probiotics supplementation was effective only on the small size *** was a significant difference in the time taken for complete resolution of small size chalazia between the two groups in favor of the patients receiving probiotics(38.50±9.04 d vs 21.00±7.00 d,P=0.039).Medium and large size chalazia did not respond to medical treatment with or without probiotics supplementation over the follow-up period(3 mo).The treatment did not induce any complications in both groups and no recurrence of chalaziosis was recorded in both ***:The considerable difference in time taken for complete resolution of small chalazia between the two groups in favor of the experimental one confirms the presence of a gut-eye axis.

关键词： microbiome probiotics chalaziosis adults

Depression and chronic heart failure in the elderly： an intriguing relationship

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Journal of Geriatric Cardiology 2018年第6期15卷 451-459页

作者： Ilaria Liguori Gennaro Russo Francesco Curcio Giuseppe Sasso David Della-Morte Gaetano Gargiulo Flora Pirozzi Francesco Cacciatore Domenico Bonaduce pasquale Abete Gianluca Testa Department of Translational Medical Sciences University of Naples 'Federico Ⅱ' Naples Italy Department of Systems Medicine University of Rome Tor Vergata Rome ltaly IRCCS San Raffaele Pisana Rome Italy Division of Internal Medicine A OU San Giovanni di Dio e Ruggi di Aragona Salerno Italy Azienda Ospedaliera dei Colli Monaldi Hospital Heart Transplantation Unit Naples Italy Department of Medicine and Health Sciences University of Molise Campobasso Italy

Chronic heart failure and depressive disorders have a high prevalence and incidence in the elderly. Several studies have shown how depression tends to exacerbate coexisting chronic heart failure and its clinical outcomes and vice versa, especially in the elderly. The negative synergism between chronic heart failure and depression in the elderly may be approached only taking into account the multifaceted pathophysiological characteristics underlying both these conditions, such as behavioural factors, neurohormonal activation, inflammatory mediators, hypercoagulability and vascular damage. Nevertheless, the pathophysiological link between these two conditions is not well established yet. Despite the high prevalence of depression in chronic heart failure elderly patients and its negative prognostic value, it is often unrecognized especially because of shared symptoms. So the screening of mood disorders, using reliable questionnaires, is recommended in elderly patients with chronic heart failure, even if cannot substitute a diagnostic interview by mental health professionals. In this setting, treatment of depression requires a multidisciplinary approach including： psychotherapy, antidepressants, exercise training and electroconvulsive therapy. Pharmacological therapy with selective serotonin reuptake inhibitors, despite conflicting results, improves quality of life but does not guarantee better outcomes. Exercise training is effective in improving quality of life and prognosis but at the same time cardiac rehabilitation services are vastly underutilized.

关键词： Chronic heart failure Depression The elderly

Positive cascading effect of restoring forests

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International Soil and Water Conservation Research 2020年第1期8卷 102-102页

作者： pasquale Borrelli Panos Panagos David Wuepper University of Basel Bernoullistrasse 304056BaselSwitzerland European Commission Joint Research Centre(JRC)Via Enrico Fermi 2749I-21027IspraVAItaly ETH Zürich Sonneggstrasse 338092ZürichSwitzerland

Recent assessment of global tree restoration potential reports that under current climate conditions there would be room for additional 0.9 billion hectares of woodlands and forests Bastin(2019).This could store 205 gigatonnes of carbon making forest restoration a viable strategy for climate change ***-menting on Bastin(2019),Chazdon and Brancalion(2019)call for holistic approaches because forest restoration is a mechanism to achieve multiple goals that go beyond climate mitigation,also including biodiversity conservation,socioeconomic benefits,food security,and ecosystem services.A timely scientific debate consid-ering the recent decision of the UN Environment Assembly in Nai-robi,Kenya,to declare the coming decade 2021-2030 the UN Decade on Ecosystem Restoration(Link 1).

关键词： Soil Forest Ecosystem restoration Carbon sequestration Climate change mitigation

Nosocomial spontaneous bacterial peritonitis antibiotic treatment in the era of multi-drug resistance pathogens: A systematic review

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World Journal of Gastroenterology 2017年第25期23卷 4654-4660页

作者： Marco Fiore Alberto Enrico Maraolo Ivan Gentile Guglielmo Borgia Sebastiano Leone pasquale Sansone Maria Beatrice Passavanti Caterina Aurilio Maria Caterina Pace Department of Anaesthesiological Surgical and Emergency SciencesUniversity of Campania“Luigi Vanvitelli” Department of Clinical Medicine and Surgery Section of Infectious DiseasesUniversity of Naples Federico II Division of Infectious Diseases “San Giuseppe Moscati”Hospital

To systematically review literature upon aetiology of nosocomial spontaneous bacterial peritonitis (N-SBP) given the rising importance of multidrug-resistant (MDR) bacteria. METHODSA literature search was performed on MEDLINE and Google Scholar databases from 2000 to 15th of November 2016, using the following search strategy: “spontaneous” AND “peritonitis”. RESULTSThe initial search through electronic databases retrieved 2556 records. After removing duplicates, 1958 records remained. One thousand seven hundred and thirty-five of them were excluded on the basis of the screening of titles and abstract, and the ensuing number of remaining articles was 223. Of these records, after careful evaluation, only 9 were included in the qualitative analysis. The overall proportion of MDR bacteria turned out to be from 22% to 73% of cases across the studies. CONCLUSIONN-SBP is caused, in a remarkable proportion, by MDR pathogens. This should prompt a careful re-assessment of guidelines addressing the treatment of this clinical entity.

关键词： Hospital-acquired infections Nosocomial spontaneous bacterial peritonitis Multidrug resistant bacteria Cirrhosis Critically ill patient

Long-term outcome of pancreatic function following oncological surgery in children:Institutional experience and review of the literature

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World Journal of Clinical Cases 2021年第25期9卷 7340-7349页

作者： Giulia Bolasco Teresa Capriati Chiara Grimaldi Lidia Monti Maria Debora De pasquale Ippolita Patrizia Patera Marco Spada Giuseppe Maggiore Antonella Diamanti Hepatology Gastroenterology and Nutrition Unit“Bambino Gesù”Children’s HospitalIRCCSRome 00165Italy Division of Hepatobiliopancreatic Surgery Liver and Kidney Transplantation"Bambino Gesù"Children's HospitalIRCCSRome 00165Italy Radiology Department "Bambino Gesù"Children’s HospitalIRCCSRome 00165Italy Hematology/Oncology Department "Bambino Gesu"Children's HospitalIRCCSRome 00165Italy Endocrinology and Diabetes Unit "Bambino Gesù"Children’s HospitalIRCCSRome 00165Italy

BACKGROUND Pancreatic neoplasms are uncommon in children and in most cases they are benign or have low malignant *** and solid pseudopapillary tumor are the most frequent types in early and late childhood,*** resection,although burdened by severe complications,is the only curative treatment for these *** surgery may result in impaired exocrine and endocrine pancreatic ***,limited data are available on the long-term pediatric pancreatic function following surgical *** To investigate endocrine and exocrine pancreatic function and growth after oncological pancreatic surgery in a pediatric *** A retrospective analysis of all pediatric patients who underwent surgery for pancreatic neoplasm in our Institution from January 31,2002 to the present was *** and exocrine insufficiency,auxological and fat-soluble vitamin status(A,D,E and clotting tests)were assessed at diagnosis and at every follow-up *** insufficiency was defined as steatorrhea with fecal elastase-1<200μg/g stool,while endocrine insufficiency was identified as onset of Diabetes or Impaired Glucose *** was evaluated based on body mass index(BMI)z-score *** Sixteen patients(12 girls and 4 boys,mean age 10.7±5.3 years),were *** patients(56%)had a neoplasm in the pancreatic head,4 in the body/tail,2 in the tail and 1 in the *** findings were as follows:Solid pseudopapillary tumor in 10 patients(62.5%),insulinoma in 2 patients,neuroendocrine tumor in 2 patients and acinar cell carcinoma in 2 *** most frequent surgery was pancreaticoduodenectomy(50%).Exocrine failure occurred in 4 patients(25%)and endocrine failure in 2 patients(12.5%).Exocrine insufficiency occurred early(within 6 mo after surgery)and endocrine insufficiency later(8 and 10 years after surgery).Mean BMI z-score was 0.36±1.1 at diagnosis and 0.27±0.95 at the last ***

关键词： Pancreatic neoplasms Pancreatic function Children Malabsorption Pancreatic failure Growth

Polymer-coated nanoporous carbons for trace seawater uranium adsorption

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Science China Chemistry 2013年第11期56卷 1510-1515页

作者： YUE YanFeng SUN XiaoGuang MAYES Richard T. KIM Jungseung FULVIO pasquale F. QIAO ZhenAn BROWN Suree TSOURIS Costas OYOLA Yatsandra DAI Sheng Chemical Sciences Division Oak Ridge National Laboratory Energy & Transportation Science Division Oak Ridge National Laboratory Department of Chemistry University of Tennessee

Polymer-coated mesoporous carbon nanocomposites were prepared from the immobilization of acrylonitrile and acrylic acid copolymers with divinylbenzene as a crosslinker onto a mesoporous carbon *** surface areas were maintained after polymerization with accessible *** functional nanocomposite was tested as an adsorbent for uranium from high salinity *** adsorption results have shown that the adsorption capacities are strongly influenced by the density of the amidoxime groups and the specific surface area.

关键词： mesoporous carbon nanocomposite copolymerization seawater uranium adsorption