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Novel CMS lines in pigeonpea[Cajanus cajan(L.)Millspaugh]derived from cytoplasmic substitutions,and their effective restoration and deployment in hybrid breeding

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The Crop Journal 2017年第1期5卷 89-94页

作者： Abhishek Bohra Rintu Jha Indra Prakash Singh Gaurav Pandey Shalini Pareek partha sarathi basu Sushil Kumar Chaturvedi Narendra Pratap Singh ICAR Indian Institute of Pulses Research(IIPR)Kanpur 208024India

The availability of stable cytoplasmic male sterile(CMS or A) lines coupled with a robust restoration system(R lines) is an essential prerequisite for efficient hybrid ***-enabled hybrid technology holds immense potential to enhance the long-stagnant productivity of pigeonpea. In the present investigation, cytoplasmic substitutions were made in the nuclear backgrounds of early-maturing pigeonpea varieties or lines. Three new CMS lines(ICPL 88039 A, Pusa 992 A, and DPP 3-2A) resulted from genetic crosses involving cytoplasmic donors from A2(GT 288A) and A4(ICPA 2089) categories. In addition to visual inspection of anthers, pollen-staining techniques and scanning electron microscopy(SEM)analysis were used to confirm pollen sterility. Further, given the relevance of the plant mitochondrial genome to CMS manifestation, 25 mitochondrion-specific DNA markers were assayed on these newly developed A lines and isogenic maintainer(B) lines. DNA polymorphism between Pusa 992 A and Pusa 992 B as revealed by the nad7a_del marker confirmed the successful combination of sterilizing cytoplasm(A4) and nonrestoring nuclear background(Pusa 992). Such cytoplasm-specific DNA markers are required for A2-CMS as well. Further, to assess restoration ability, potential restorers were crossed with these CMS lines, and as a consequence, promising A × R combinations exhibiting 100%pollen fertility could be identified. In parallel, we also analyzed the inheritance patterns underlying fertility restoration using ICPL 88039A-derived F2 and BC1F1populations,and established a monogenic dominant model to explain the phenomenon of A2-CMS restoration. In summary, we report the successful development of new CMS lines and describe their effective deployment in hybrid breeding of pigeonpea.

关键词： Cytoplasm Hybrid Mitochondria Pigeonpea Restorer Sterility

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Non-Nutritive Bioactive Compounds in Pulses and Their Impact on Human Health: An Overview

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Food and Nutrition Sciences 2012年第12期3卷 1664-1672页

作者： Jagdish Singh partha sarathi basu Division of Basic Sciences Indian Institute of Pulses Research Kanpur India

This review describes the non-nutritive biologically active components in grain legumes and discusses about the bioactivity of phenols, isoflavones, phytosterols, phytic acid, saponins, tannins, protease inhibitors and bioactive complex carbohydrates in different pulse grains. These bioactive components have wide ranging biological activities and conesquently many different targets and mechanism of action. The potential beneficial effect of these compounds especially their antioxidant properties and their role in the prevention of non-communicable chronic diseases such as coronary heart disease, stroke, cancer and diabetes has been discussed.

关键词： Pulses Grain Legumes Bioactive Compounds Anti Nutritional Compounds Phenolics Antioxidants An-tioxidant Activity

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Pulsed Magnetic Field Measurement Outside Finite Length Solenoid: Experimental Results &Mathematical Verification

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Journal of Electromagnetic Analysis and Applications 2013年第10期5卷 371-378页

作者： Shibaji basu Siddhartha Shankar Pany partha Bannerjee Sabyasachi Mitra Accelerator & Pulse Power Division Bhabha Atomic Research Centre Mumbai India Accelerator & Pulse Power Division Bhabha Atomic Research Centre Vishakapatnam India Electronics & Instrumentation Services Division Bhabha Atomic Research Centre Mumbai India Energetics & Electromagnetics Division Bhabha Atomic Research Centre Vishakapatnam India

The paper deals with magnetic field mapping outside a finite length solenoid electromagnet, by an in-house designed and calibrated inductive pick-up or search coil. The search coil is calibrated in a unique methodology based on the azimuthal magnetic field component generated by a straight wire. This unique calibration technique helps us to avoid additional circuitry to integrate the signal obtained from search coil. The methodology proves advantageous in diffusion, implosion studies where the signal frequency changes with dimension and material of experimental job-piece (hollow metal tube). Remedial measures have been taken to avoid electrostatic capacitive pick-up (which eventually exacerbates with integration) keeping measurement simple and accurate. The experimentally measured field values have also been compared with electromagnetic field results obtained from mathematical calculations and finite element based simulations. Two different mathematical approaches have been demonstrated for field computation based on Biot-Savart Law. Both the methods have taken into account the exact geometry of the solenoid, including the inter-turn gaps. The methods use appropriate combination of closed-form mathematical expression and numerical integration techniques and are capable of determining all the vector components of magnetic field anywhere around the finite length solenoid. The mathematical computations are equally significant contributions in the paper especially because exact determination of magnetic fields outside finite length solenoids has not been discussed in sufficient specific details in already existing literature. The mathematical computations, finite element simulations and experimental verification together provide a holistic solution to magnetic field determination problems in pulse power applications that have not been discussed in available literature or books in specific details.

关键词： Pulsed Magnetic Field Biot-Savart Law Search Coil

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Human Papillomavirus Type-Distribution in Indian Women with and without cervical lesions:A Meta-Analysis

Human Papillomavirus Type-Distribution in Indian Women with ...

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24th International Papillomavirus Conference and Clinical Workshop

作者： Neena Lal Bhatla Neerja basu partha Ng Timothy L Bock Hans L All India Institute of Medical Sciences New DelhiIndia All India Institute of Medical Sciences New DelhiIndia Chittaranjan National Cancer Institute KolkataIndia GlaxoSmithKline Biologicals RixensartBelgium GlaxoSmithKline Biologicals RixensartBelgium

＜正＞Objectives:To determine the human papillomavirus（HPV）prevalence and type-distribution in women from south Asia with and without cervical lesions and to estimate the future impact of an HPV 16/18 prophylactic v...

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Significant increase in HBV, HCV, HIV and syphilis infections among blood donors in West Bengal, Eastern India 2004-2005: Exploratory screening reveals high frequency of occult HBV infection

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World Journal of Gastroenterology 2007年第27期13卷 3730-3733页

作者： Prasun Bhattacharya partha Kumar Chandra Sibnarayan Datta Arup Banerjee Subhashish Chakraborty Krishnan Rajendran Subir Kumar basu Sujit Kumar Bhattacharya Runu Chakravarty Institute of Blood Transfusion Medicine and Immunohematology Kolkata India ICMR Virus Unit Kolkata India National Institute of Cholera and Enteric Diseases Kolkata India

AIM: To evaluate the prevalence of markers of hepatitis B virus (HBV) and hepatitis C virus (HCV) and human immunodeficiency virus (HIV) among blood donors in Kolkata, Eastern India for two consecutive years and to conduct a pilot study to explore the presence of HBV DNA among hepatitis B surface antigen (HBsAg) negative but anti-HBc positive blood donors. METHODS: Seroprevalence of HBsAg, anti-HCV and anti-HIV was studied among 113 051 and 106 695 voluntary blood donors screened in 2004 and 2005, respectively. Moreover, a pilot study on 1027 HBsAg negative donors was carried out for evaluating the presence of HBV DNA by PCR on HBsAg negative/anti- HBc positive donors. RESULTS: A statistically significant increase in the prevalence of HBV (1448 vs 1768, P < 0.001), HIV (262 vs 374, P < 0.001), HCV (314 vs 372, P = 0.003) and syphilis (772 vs 853, P = 0.001) infections was noted among blood donors of Kolkata West Bengal in 2005 as compared to 2004. Moreover, the exploratory study on 1027 HBsAg negative donors revealed that 188 (18.3%)of them were anti-HBc positive out of which 21% were positive for HBV DNA. CONCLUSION: The findings of this study underscore the significantly increasing endemicity of hepatitis viruses, syphilis and HIV among the voluntary blood donors of our community. The pilot study indicates a high rate of prevalence of HBV DNA among HBsAg negative/anti-HBc positive donors and thus emphasizes the need for a more sensitive and stringent screening algorithm for blood donations.

关键词： Hepatitis B virus Human immunodeficiency virus Hepatitis C virus Blood donation Occult HBV infection

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