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Immunological-based assays for specific detection of shrimp viruses

World Journal of Virology 2014年第1期3卷 1-10页

作者： parin chaivisuthangkura Siwaporn Longyant Paisarn Sithigorngul Department of Biology Faculty of Science Srinakharinwirot University Bangkok 10110 Thailand Faculty of Environmental Culture and Ecotourism Srinakharinwirot University Bangkok 10110 Thailand

Among shrimp viral pathogens, white spot syndrome virus(WSSV) and yellow head virus(YHV) are the most lethal agents, causing serious problems for both the whiteleg shrimp, Penaeus(Litopenaeus) vannamei, and the black tiger shrimp, Penaeus(Penaeus) monodon. Another important virus that infects P. vannamei is infectious myonecrosis virus(IMNV), which induces the white discoloration of affected muscle. In the cases of taura syndrome virus and Penaeus stylirostris densovirus(Pst DNV; formerly known as infectious hypodermal and hematopoietic necrosis virus), their impacts were greatly diminished after the introduction of tolerant stocks of P. vannamei. Less important viruses are Penaeus monodon densovirus(Pm DNV; formerly called hepatopancreatic parvovirus), and Penaeus monodon nucleopolyhedrovirus(Pemo NPV; previously called monodon baculovirus). For freshwater prawn, Macrobrachium rosenbergii nodavirus and extra small virus are considered important viral pathogens. Monoclonal antibodies(MAbs) specific to the shrimp viruses described above have been generated and used as an alternative tool in various immunoassays such as enzyme-linked immunosorbent assay, dot blotting, Western blotting and immunohistochemistry. Some of these MAbs were further developed into immunochromatographic strip tests for the detection of WSSV, YHV, IMNV and Pemo NPV and into a dual strip test for the simultaneous detection of WSSV/YHV. The strip test has the advantages of speed, as the result can be obtained within 15 min, and simplicity, as laboratory equipment and specialized skills are not required. Therefore, strip tests can be used by shrimp farmers for the pond-side monitoring of viral infection.

关键词： Shrimp viruses Immunological assay Western blot Immunohistochemistry Immunochromatographic strip test

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