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园艺学报 2017年第5期44卷 817-827页

作者：樊胜雷超辛明志邵红霞 Muhammad Mobeen tahir 张东韩明玉西北农林科技大学园艺学院陕西杨凌712100

以‘长富2号’苹果为试验材料,从其短枝顶芽中克隆得到1个赤霉素信号转导因子mdGAMYB,对其进行生物信息学和表达分析。结果表明,mdGAMYB的开放阅读框(ORF)长度为1 656bp,编码551个氨基酸,蛋白质分子量为59.741 kD。生物信息学分析表明md... 详细信息

以‘长富2号’苹果为试验材料,从其短枝顶芽中克隆得到1个赤霉素信号转导因子mdGAMYB,对其进行生物信息学和表达分析。结果表明,mdGAMYB的开放阅读框(ORF)长度为1 656bp,编码551个氨基酸,蛋白质分子量为59.741 kD。生物信息学分析表明mdGAMYB编码的蛋白存在多个糖基化位点和磷酸化位点;序列分析表明,md GAYMB和其他物种的GAMYB蛋白有很高的相似性,均含有保守的R2R3 DNA结合域和GAMYB家族所特有的Box1,Box2和Box3保守区域;系统进化分析表明,md GAYMB与梨、梅花、草莓、枣和葡萄等的GAMYB蛋白具有较高的同源性。实时荧光定量PCR分析表明,md GAYMB具有组织表达特异性,在叶片、花和芽中的表达量较高。外源GA3处理抑制了花芽孕育和翌年成花,抑制mdGAMYB的表达。在易成花品种‘烟富6号’中的表达量高于难成花品种‘长富2号’。

关键词：苹果 mdGAMYB 基因克隆基因表达成花诱导

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Endoscopic pancreatic sphincterotomy:indications,outcome,and a safe stentless technique

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World Journal of Gastroenterology 1998年第3期4卷 57-57页

作者： Eric Elton, md, Douglas A. Howell, md, Willis G. Parsons, md, tahir Qaseem, md, Brian L. Hanson, mdPortland, MaineDivision of Gastroenterology, Maine Medical Center, Portland, Maine

AIM Endoscopic pancreatic sphincterotomy is less widely practiced than biliary sphincterotomy, in part because of the lack of firm data regarding its indications and safety. In addition, recent reports of ductal and parenchymal changes occurring after pancreatic stenting raise concerns about the standard practice of stent placement at the time of pancreatic sphincterotomy. We report our experience with pancreatic sphincterotomy and describe the use of a technique involving overnight nasopancreatic drainage rather than stenting.METHODS We reviewed the records of the 164 pancreatic sphincterotomies performed on 160 patients at our institution between January 1, 1991, and October 1, 1996, comparing procedures done with overnight nasopancreatic catheter placement with those done with stenting or no drainage. We also examined the longterm clinical outcome of patients after pancreatic sphincterotomy.RESULTS Of the 164 sphincterotomies, 98 were done with overnight nasopancreatic drainage, 50 with stent placement, and 16 with no drainage. Complications (all pancreatitis) were significantly more frequent in the group with no drainage (125%) as compared with those with drainage (07%); P<0003. Nasopancreatic drainage was as safe as stent placement, with no complications after 98 procedures. Pancreatic sphincterotomy was effective when used as primary therapy, with 64% of patients so treated experiencing complete and longlasting resolution of symptoms after the procedure.CONCLUSION Pancreatic sphincterotomy is safe and effective, although pancreatic drainage is required to reduce the incidence of pancreatitis. Overnight nasopancreatic drainage is the method of choice, as it carries as low a complication rate as stent placement, but without the need for a repeat procedure, and presumably without the risk of ductal and parenchymal damage.

关键词： sphincterotomy indications

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The immunogenic maturation of goat monocyte-derived dendritic cells and upregulation of toll-like receptors by five antigens of Haemonchus contortus in-vitro

The immunogenic maturation of goat monocyte-derived dendriti...

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2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛

作者： Muhammad Waqqas Hasan Muhammad Haseeb Muhammad Ehsan Javaid Ali Gadahi Qiangqiang Wang Muhammad Ali Memon Muhammad tahir Aleem Shakeel Ahmed Lakho Ruo Feng Yan Li Xin Xu Xiao Kai Song 李祥瑞 College of Veterinary Medicine Nanjing Agricultural University

Previously,it was found that several proteins of Haemonchus contortus were involved in the stimulation of the host immune ***,the information about the selection of superlative antigens with immunogenic efficacies on host DCs is *** the current study,the stimulatory effects of five recombinant proteins(elongation factor-1α,arginine kinase,ES-15,ES-24,and ADP-ribosylation factor 1) of *** on the maturation of goat monocyte-derived dendritic cells(md-DCs) were *** proteins were purified separately in *** expression and incubated with isolated goat peripheral blood mononuclear cells(PBMC).Immunofluorescence assay(IFA) results confirmed the binding of these molecules to the md-DC’s surface as compared to control *** the flow cytometry analysis,recombinant proteins induced md-DC stimulation via the up-regulation of the expression of the costimulatory molecule(CD80) and MHC-Ⅱ.Quantitative RT-PCR data showed a significant increase in the expression of specific genes of the WNT and toll-like receptor(TLR) signaling *** result of ELISA indicated the higher levels of cytokine(IL-10,IL-12,IFN-y,and TNF-α) secretion in the md-DC compared to the negative(pET-32 a His-Tag) and blank(PBS)control *** data gives valuable support in the selection of potential antigens for future studies on the immunomodulation of the host against the infection of ***.

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