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Endoscopic ultrasound-guided fine-needle aspiration cytology in pancreaticobiliary carcinomas:diagnostic efficacy of cell-block immunocyto-chemistry

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Hepatobiliary & Pancreatic Diseases International 2015年第3期14卷 305-312页

作者： Antonio Ieni paolo todaro Stefano Francesco Crinò Valeria Barresi Giovanni Tuccari Department of Human Pathology "Gaetano Barresi" Section of Pathological AnatomyAzienda Ospedaliera Universitaria "Policlinico G. Martino" University of Messina Department of Internal Medicine Digestive Endoscopy UnitAzienda Ospedaliera Universitaria "Policlinico G. Martino" University of Messina

BACKGROUND： Endoscopic ultrasound-guided fine-needle aspiration cytology was demonstrated to be a useful tool for the diagnosis and staging of pancreaticobiliary neoplastic le- sions. Nonetheless, the diagnostic value of this procedure may be limited by low cellularity of the specimen, contamination of intestinal cells and unfeasibility of ancillary immunocy- tochemical procedures. The present study was to evaluate its usefulness in the diagnosis of neoplastic lesions.

关键词： endoscopic ultrasound fine-needle aspiration cytology pancreas cell-block contaminant cells immunohistochemistry

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Cell-block procedure in endoscopic ultrasound-guided-fine-needle-aspiration of gastrointestinal solid neoplastic lesions

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World Journal of Gastrointestinal Endoscopy 2015年第11期7卷 1014-1022页

作者： Antonio Ieni Valeria Barresi paolo todaro Rosario Alberto Caruso Giovanni Tuccari Department of Human Pathology "Gaetano Barresi" Section of Anatomic Pathology University of MessinaAzienda Ospedaliera Universitaria "Gaetano Martino" 98125 Messina Italy Department of Human Pathology "Gaetano Barresi" Section of Cytopathology University of MessinaAzienda Ospedaliera Universitaria "Gaetano Martino" 98125 Messina Italy

In the present review we have analyzed the clinical applications of endoscopic ultrasound-guided-fineneedle-aspiration(EUS-FNA) and the methodological aspects obtained by cell-block procedure(CBP) in the diagnostic approach to the gastrointestinal neoplastic pathology. CBP showed numerous advantages in comparison to the cytologic routine smears; in particular, better preservation of cell architecture, achievement of routine haematoxylin-eosin staining equivalent to histological slides and possibility to perform immunohistochemistry or molecular analyses represented the most evident reasons to choose this method. Moreover, by this approach, the differential diagnosis of solid gastrointestinal neoplasias may be more easily achieved and the background of contaminant nonneoplastic gastrointestinal avoided. Finally, biological samples collected by EUS-FNA CBP-assisted should be investigated in order to identify and quantify further potential molecular markers.

关键词： Endoscopic ultrasound-guided-fine-needleaspiration

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