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The impact of gut microbiota changes on the intestinal mucus barrier in burned mice:a study using 16S rRNA and metagenomic sequencing

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Burns & Trauma 2023年第1期11卷 842-856页

作者： Xule Zha Sen Su Dan Wu panyang zhang Yan Wei Shijun Fan Qianying Huang Xi Peng Clinical Medical Research Center Southwest HospitalThird Military Medical University(Army Medical University)Gaotanyan StreetShapingba DistrictChongqing400038China State Key Laboratory of Trauma Burns and Combined InjurySouthwest HospitalThird Military Medical University(Army Medical University)Chongqing400038China

Background:The gut microbiota is a complex ecosystem that plays a critical role in human health and ***,the relationship between gut microbiota and intestinal damage caused by burns is not well *** intestinal mucus layer is crucial for maintaining intestinal homeostasis and providing a physiological barrier against bacterial *** study aims to investigate the impact of gut microbiota on the synthesis and degradation of intestinal mucus after burns and explore potential therapeutic targets for burn ***:A modified histopathological grading system was employed to investigate the effects of burn injury on colon tissue and the intestinal mucus barrier in ***,16S ribosomal RNA sequencing was used to analyze alterations in the gut microbiota at days 1-10 *** on this,metagenomic sequencing was conducted on samples collected at days 1,5 and 10 to investigate changes in mucus-related microbiota and explore potential underlying ***:Our findings showed that the mucus barrier was disrupted and that bacterial translocation occurred on day 3 following burn injury in ***,the gut microbiota in mice was significantly disrupted from days 1 to 3 following burn injury,but gradually recovered to normal as the disease ***,there was a marked increase in the abundance of symbiotic and pathogenic bacteria associated with mucin degradation on day 1 after burns,but the abundance returned to normal on day ***,the abundance of probiotic bacteria associated with mucin synthesis changed in the opposite *** analysis revealed that after a burn injury,bacteria capable of degrading mucus may utilize glycoside hydrolases,flagella and internalins to break down the mucus layer,while bacteria that synthesize mucus may help restore the mucus layer by promoting the production of short-chain fatty ***:Burn injury leads to disruption of colonic mucus barrier an

关键词： Burn injury Gut microbiota Intestinal mucus 16S rRNA Metagenomic sequencing

Heteroatoms doped iron oxide-based catalyst prepared from zinc slag for efficient selective catalytic reduction of NO_(x) with NH_(3)

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International Journal of Coal Science & Technology 2024年第1期11卷 180-193页

作者： Jiale Liang Yaojun zhang Hao Chen Licai Liu panyang He Lei Wu College of Materials Science and Engineering Xi’an University of Architecture and TechnologyXi’an710055China School of Chemistry and Chemical Engineering Xi’an University of Architecture and TechnologyXi’an710055China School of Resources Engineering Xi’an University of Architecture and TechnologyXi’an710055China

Excessive emissions of nitrogen oxides from flue gas have imposed various detrimental impacts on environment,and the development of deNO_(x) catalysts with low-cost and high performance is an urgent *** oxide-based material has been explored for promising deNO_(x) ***,the unsatisfactory low-temperature activity limits their practical *** this study,a series of excellent low-temperature denitrification catalysts(Ha-FeO_(x)/yZS)were prepared by acid treatment of zinc slag,and the mass ratios of Fe to impure ions was regulated by adjusting the acid ***-FeO_(x)/yZS showed high denitrification performance(>90%)in the range of 180–300℃,and the optimal NO conversion and N2 selectivity were higher than 95%at 250℃.Among them,the Ha-FeO_(x)/2ZS synthesized with 2 mol/L HNO3 exhibited the widest temperature window(175–350℃).The excellent denitrification performance of Ha-FeO_(x)/yZS was mainly attributed to the strong interaction between Fe and impurity ions to inhibit the growth of crystals,making Ha-FeO_(x)/yZS with amorphous structure,nice fine particles,large specific surface area,more surface acid sites and high chemisorbed *** in-situ DRIFT experiments confirmed that the SCR reaction on the Ha-FeO_(x)/yZS followed both Langmuir-Hinshelwood(L-H)mechanism and Eley-Rideal(E-R)*** present work proposed a high value-added method for the preparation of cost-effective catalysts from zinc slag,which showed a promising application prospect in NO_(x) removal by selective catalytic reduction with ammonia.

关键词： Flue gas NO_(x)removal Zinc slag-derived catalyst NH_(3)-SCR Catalysis activity

长链非编码RNA Rpph1对高糖培养的肾小球系膜细胞炎症因子表达的影响

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第三军医大学学报 2019年第1期41卷 33-40页

作者：张攀扬孙艳彭睿彭惠民张政重庆医科大学基础医学院细胞生物学及遗传学教研室重庆400016 重庆医科大学基础医学院分子医学与肿瘤研究中心重庆400016

目的探讨长链非编码RNA (long non-coding RNA,lncRNA) Rpph1对糖尿病肾病(diabetic nephropathy,DN)肾小球系膜细胞炎症因子的影响。方法采用荧光原位杂交(fluorescence in situ hybridization,FISH)检测Rpph1细胞定位,实时定量PCR (quantitative real-time PCR,qRT-PCR)检测Rpph1在DN小鼠肾脏组织和系膜细胞中的水平;生物信息学技术预测Rpph1的编码蛋白能力。构建Rpph1的过表达质粒,并设计合成Rpph1的siRNAs,用qRT-PCR验证其转染效率。在高糖培养的肾小球系膜细胞中转染Rpph1-siRNA;在低糖培养的肾小球系膜细胞中转染Rpph1过表达质粒,通过Western blot检测转染Rpph1的siRNA和过表达Rpph1后肿瘤坏死因子(tumor necrosis factorα,TNF-α)以及单核细胞趋化蛋白-1(macrophage cationic peptide 1,MCP-1)的表达情况。结果与正常小鼠比较,Rpph1在DN小鼠肾脏组织中显著上调(P <0. 01);同样,高糖培养的系膜细胞中Rpph1表达较低糖培养的系膜细胞显著上调(P <0. 01),其主要定位于系膜细胞的细胞质。此外,生物信息学证实Rpph1不具有编码蛋白的能力。且过表达Rpph1后炎症相关因子TNF-α和MCP-1的水平明显上调(P <0. 05);在下调Rpph1后,炎症相关因子TNF-α和MCP-1的表达也随之下降(P <0. 05)。结论在DN小鼠肾脏组织和高糖环境下的系膜细胞中lncRNA Rpph1显著高表达,且增强炎症相关因子的表达。Rpph1可能与糖尿病肾病炎症的发生相关。

关键词：糖尿病肾病长链非编码RNA Rpph1 肾小球系膜细胞炎症

半乳糖凝集素3调控高糖下肾小球系膜细胞增殖的研究

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第三军医大学学报 2020年第19期42卷 1913-1919页

作者：林子越张攀扬崇馨煜孙艳彭睿张政重庆医科大学基础医学院:细胞生物学及遗传学教研室重庆400016 重庆医科大学基础医学院:生物信息学教研室重庆400016

目的探究半乳糖凝集素3(galectin-3,Gal-3)对高糖培养的肾小球系膜细胞(mesangial cells,MCs)增殖的作用及机制。方法实时定量PCR(qRT-PCR)检测Gal-3在糖尿病肾病(diabetic nephropathy,DN)、正常小鼠肾脏组织和高低糖条件下MCs中的表达... 详细信息

目的探究半乳糖凝集素3(galectin-3,Gal-3)对高糖培养的肾小球系膜细胞(mesangial cells,MCs)增殖的作用及机制。方法实时定量PCR(qRT-PCR)检测Gal-3在糖尿病肾病(diabetic nephropathy,DN)、正常小鼠肾脏组织和高低糖条件下MCs中的表达;构建、合成Gal-3过表达质粒和siRNA,qRT-PCR和Western blot检测转染效率;在高糖培养的MCs中转染Gal-3-siRNA;在低糖培养的MCs中转染Gal-3过表达质粒,通过5-乙基-2′-脱氧尿嘧啶核苷(5-Ethynyl-2′-deoxyuridine, EdU)检测MCs的增殖能力,通过Western blot检测高低表达Gal-3对Mek和磷酸化Mek表达的影响。结果 qRT-PCR结果显示:与正常小鼠相比,Gal-3在DN小鼠肾脏组织中表达显著升高(P<0.01),且与低糖下的MCs相比,高糖条件下的MCs中Gal-3表达显著升高(P<0.01)。过表达Gal-3后,MCs增殖能力显著提高(P<0.01)且磷酸化Mek表达上调(P<0.01),而在siRNA沉默Gal-3后,MCs增殖能力显著降低(P<0.01)且磷酸化Mek表达下调(P<0.001)。结论 Gal-3在DN中显著上调,并且可能通过调控Mek磷酸化影响MCs的增殖,进而在DN中发挥重要的调节作用。

关键词：糖尿病肾病 galectin-3 肾小球系膜细胞细胞增殖 Mek

甘氨酸与热力学抑制剂对甲烷水合物的协同抑制机理

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天然气工业 2024年第8期44卷 107-113页

作者：汪杰张良君刘成郭盼阳江厚顺马攀付宏雨非常规油气省部共建协同创新中心·长江大学油气钻采工程湖北省重点实验室·长江大学长江大学石油工程学院中国石油浙江油田公司油气勘探开发项目经理部中国石油辽河油田公司金海采油厂

天然气水合物在开采过程中,温度和压力的变化会导致分解后的水合物二次形成,进而会造成井筒和管线堵塞。甘氨酸因其强亲水性和生物可降解性,在水合物防治方面具有巨大应用潜力,但其与热力学抑制剂复配协同抑制水合物生成的作用机理尚不... 详细信息

天然气水合物在开采过程中,温度和压力的变化会导致分解后的水合物二次形成,进而会造成井筒和管线堵塞。甘氨酸因其强亲水性和生物可降解性,在水合物防治方面具有巨大应用潜力,但其与热力学抑制剂复配协同抑制水合物生成的作用机理尚不明确。为此,开展了甘氨酸与常见盐类和醇类热力学抑制剂复配使用时的甲烷水合物生成模拟实验,分析了甘氨酸与热力学抑制剂协同抑制甲烷水合物生成机理,并形成了适用于海洋水合物钻探开发用的甘氨酸类钻井液体系。研究结果表明:①甲烷气消耗量并不能真实反映抑制剂对水合物生成的抑制效果,还需结合水合物最终生成量、水合物生成3个阶段的时间变化情况综合判断;②甘氨酸浓度为1.0%时,抑制水合物生成的效果最佳,对应的反应体系具有最小的水合物生成区和最大的稳定区;③相比于单独使用1.0%甘氨酸,5.0%盐类抑制剂与1.0%甘氨酸混合使用时,具有协同抑制水合物生成的效果,可减少水合物生成量20%~30%,但5.0%醇类抑制剂与1.0%以及0.5%甘氨酸混合使用时反而促进了水合物生成;④甘氨酸与盐类混合形成的钻井液体系,可以有效降低水合物生成量。结论认为,该认识进一步厘清了甘氨酸与热力学抑制剂对水合物的协同抑制机理,为解决水合物开采过程中井筒堵塞造成的钻井安全问题提供了实验数据和理论支撑。

关键词：甲烷水合物甘氨酸热力学抑制剂协同效果影响因素钻井液体系基础物性抑制性能

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One-Step Synthesis of Magnetic Zeolite from Zinc Slag and Circulating Fluidized Bed Fly Ash for Degradation of Dye Wastewater

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Journal of Renewable Materials 2020年第4期8卷 405-416页

作者： Zhichao Han Yaojun zhang panyang He College of Materials Science and Engineering Xi’an University of Architecture and TechnologyXi’an710055China

In this study,a magnetic P zeolite was directly synthesized by utilization of industrial solid wastes of zinc slag(ZS)and circulating fluidized bed fly ash(CFBFA)via one-step hydrothermal *** effects of different CFBFA/ZS ratios and hydrothermal times on the as-synthesized zeolite were *** X-ray diffraction(XRD)and vibrating sample magnetometer(VSM)results indicated that the magnetic P zeolite possessed well-defined crystals and *** as-prepared zeolite was employed as a Fenton-like solid catalyst for degradation of direct green B dye *** was discovered that the magnetic P zeolite took the advantage of rapid separation and efficient recovery under the external magnets in a solid-liquid *** effects of the solution pH,the catalyst dosage,and the H_(2)O_(2)concentration on the degradation rate of direct green B dye wastewater were studied *** results showed that the highest degradation of 96.3%was obtained and the magnetic P zeolite showed excellent stability after four ***,the magnetic P zeolite derived from industrial solid wastes had a potential application in wastewater treatment.

关键词： Magnetic zeolite solid wastes Fenton-like reaction dye degradation

An α-Fe_(2)O_(3)/Circulating Fluidized Bed Fly Ash Based Geopolymer Composite Anode for Electrocatalytic Degradation of Indigo Carmine Dye Wastewater

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Journal of Renewable Materials 2021年第12期9卷 2277-2289页

作者： Jiaqian Lei Yaojun zhang panyang He College of Materials Science and Engineering Xi’an University of Architecture and TechnologyXi’an710055China

Geopolymers have been developed to various catalysts due to their ***,low conductivity restricts their application in the electrocatalysis *** this study,anα-Fe_(2)O_(3)/circulating fluidized bed fly ash based geopolymer(CFAG)composite anode was fabricated using a facile dip-coating method by loadingα-Fe_(2)O_(3) in the matrix of *** effects ofα-Fe_(2)O_(3) content on the composition,surface morphology and electrochemical performance ofα-Fe_(2)O_(3)/CFAG composite anode were *** X-ray diffraction(XRD)and scanning electron microscope(SEM)results demonstrated thatα-Fe_(2)O_(3) was successfully inlaid with the surface of amorphous CFAG *** electrochemical measurements indicated thatα-Fe_(2)O_(3)/CFAG composite anode had higher oxygen evolution potential,greater electrochemical activity area,and smaller electrochemical impedance than *** as-prepared composite anode was applied for electrocatalytic degradation of indigo carmine dye *** was discovered that the highest degradation efficiency over 10α-Fe_(2)O_(3)/CFAG reached up 92.6%,and the degradation of indigo carmine followed pseudo-first-order ***,10α-Fe_(2)O_(3)/CFAG composite anode presented excellent stability after five *** active hydroxyl radical was generated over theα-Fe_(2)O_(3)/CFAG composite anode,which acted as strong oxidizing agents in the electrocatalytic degradation process.

关键词： Geopolymers composite anode electrocatalytic oxidation dye degradation

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补骨脂酚对去势导致雄性小鼠骨质疏松的治疗

实验动物科学 2019年第3期36卷 1-5页

作者：张盼阳王昭昕袁晓美毕亚男张玥周昆天津中医药大学中医药研究院天津300193 天津中医药大学方剂学教育部重点实验室天津300193

目的观察补骨脂酚对去势导致的雄性小鼠骨质疏松的治疗作用。方法 ICR雄性小鼠随机分为5组,分别为对照组、去势模型组、阳性药组、补骨脂酚10 mg/kg和20 mg/kg体质量组,对照组做假手术,其余组均切除小鼠睾丸。造模成功6周后,小鼠给药8... 详细信息

目的观察补骨脂酚对去势导致的雄性小鼠骨质疏松的治疗作用。方法 ICR雄性小鼠随机分为5组,分别为对照组、去势模型组、阳性药组、补骨脂酚10 mg/kg和20 mg/kg体质量组,对照组做假手术,其余组均切除小鼠睾丸。造模成功6周后,小鼠给药8周取材,检测血清生化指标,取各组右腿胫骨进行MicroCT扫描,取各组小鼠股骨进行骨生物力学测定。结果对照组、模型组、B10和B20 mg/kg组的骨钙素OC值分别为(177.69±45.13),(220.35±25.08),(167.48±74.11)和(108.76±38.17);对照组、模型组、阳性药组、B10和B20 mg/kg组的Tb.N分别为(3.53±0.49)、(1.71±0.16)、(2.51±0.35)、(2.14±0.23)和(2.45±0.42)。结论补骨脂酚对去势导致雄性小鼠骨质疏松有治疗作用。

关键词：骨质疏松补骨脂酚雄性小鼠去势

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南方医科大学学报 2023年第1期43卷 8-16页

作者：张攀扬何明敏曾园媛蔡雄伟重庆市妇幼保健院(重庆医科大学附属妇女儿童医院)妇产科重庆401147 陆军军医大学第一附属医院妇产科重庆400038

目的探讨在高级别浆液性卵巢癌(HGSOC)中的潜在功能性miRNA-mRNA调控网络与复发的相关性及其生物学意义。方法使用来自癌症和肿瘤基因图谱(TCGA)数据库的HGSOC患者样本表达数据,根据基因本体论生物过程(GO_BP)将HGSOC患者分成不同的亚型... 详细信息

目的探讨在高级别浆液性卵巢癌(HGSOC)中的潜在功能性miRNA-mRNA调控网络与复发的相关性及其生物学意义。方法使用来自癌症和肿瘤基因图谱(TCGA)数据库的HGSOC患者样本表达数据,根据基因本体论生物过程(GO_BP)将HGSOC患者分成不同的亚型,分析不同亚型与复发的相关性;通过基因表达综合(GEO)数据库找到两个与复发相关的数据集,与TCGA数据取交集,获得共同差异表达的miRNAs;预测miRNAs的靶基因,构建与HGSOC复发相关的关键miRNA-mRNA网络;通过RT-qPCR及Western blot检测miR-506-3p与SNAI2的表达水平;双荧光素酶实验验证miR-506-3p与SNAI2的靶向结合;划痕实验及Trans well实验检测miR-506-3p对卵巢癌细胞迁移与侵袭的影响。结果共筛选出与HGSOC相关通路活性的303个GO通路,确定两个亚型C1和C2。亚型与复发相关,C1患者的复发概率显著高于C2患者。C1和C2亚型之间的差异表达基因主要富集在上皮间质转化(EMT)通路,GSE25204、GSE73582及TCGA 3个数据集中有5个共同差异表达的miRNAs,共有41个靶基因能在C1和C2亚型之间差异表达的EMT通路中找到,利用这5个miRNAs及41个mRNAs构建与HGSOC复发相关的关键miRNA-mRNA网络。MiR-506-3p与SNAI2靶向结合,上调miR-506-3p抑制了SNAI2表达及降低SKOV3和CAOV3的迁移及侵袭(P<0.05);而敲低miR-506-3p显著上调SNAI2表达水平及增强SKOV3和CAOV3的迁移及侵袭(P<0.05)。结论miR-506-3p和SNAI2是与HGSOC复发相关的关键分子,miR-506-3p可能通过SNAI2调控卵巢癌细胞的迁移和侵袭影响EMT,对HGSOC的复发有预测价值。

关键词：高级别浆液性卵巢癌复发上皮间质转化 miRNA