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Isolation and Identification of Selenium-tolerant Strains from Selenium-rich Soil in Guangxi

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Agricultural Biotechnology 2018年第3期7卷 122-125,132页

作者： Qing LIAO panxia liang Ying XING Taiqing HUANG Yongxian LIU Zepu JIANG Agricultural Resource and Environment Research Institute Guangxi Academy of Agricultural Sciences

To provide reference for selenium resource exploitation of soil,many selenium-tolerant strains were isolated from selenium-rich soil in Guangxi. The dilution spread plate method and selenium-added culture method were used to screen the selenium-tolerant strains from the soils which were sampled from the main Selenium-rich areas such as Yongfu,Bama,Yulin Hanshan,Guiping and Tengxian. The results showed that 8 strains with high selenium tolerance were obtained,which could tolerate the selenium concentration above 10 000 μg/m L in solid medium. Among the 8 strains,YLB1-33 showed the highest selenium tolerance,and it could still grow weakly in the solid medium with selenium concentration of 29 000 μg/m L. The sequencing of 16 S rD NA and phylogenetic analysis showed that YLB1-6 was identified as Bacillus cereus,BMB2-1 and TXB1-8 were identified as Bacillus pumilus,GPB2-5 was identified as Bacillus thuringiensis,YLB1-26 and YLB1-33 were identified as Bacillus licheniformis,and YLB1-2 and YFB1-8 were identified as Serratia marcescens. The finding of selenium-tolerance strains had potential application value on promoting the utilization of selenium soil resources and the development of selenium-rich agricultural products in Guangxi.

关键词： Selenium-rich soil Selenium tolerance Strains Identification Guangxi

Effects of Slow-release Fertilizers and Sugarcane-specific Fertilizers on Yield and Quality of Sugarcane

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Asian Agricultural Research 2018年第6期10卷 72-74,84页

作者： Qing LIAO Zepu JIANG Ying XING Shao'e YANG Guifen CHEN panxia liang Institute of Agricultural Resource and Environment Guangxi Academy of Agricultural Sciences

The effects of slow-release fertilizers and sugarcane-specific fertilizers on the growth and quality of sugarcane were studied to provide a theoretical basis for cultivation of sugarcane with lower costs but higher efficiency. Field experiments were carried out in two major sugarcane areas in Guangxi and three fertilization treatments were studied: single application of compound fertilizers( treatment I),compound fertilizers + slow-release fertilizers( treatment II) and sugarcane-specific base fertilizers + sugarcane-specific topdressing( treatment III). Effects of equal fertilization conditions of treatment I,II and III on growth,yield and sugar of sugarcane were studied. The three fertilization treatments had little effects on emergence,tillering,and effective stems of sugarcane,but compared with the treatment of compound fertilizer( treatment I) with a ratio of N,P,and K of 1∶1∶1,treatment II using slow-release fertilizers as topdressing had better growth,higher plant height and stem diameter,so the yield was higher. Treatment III designed sugarcane-specific fertilizers with proper ratio according to fertilizer demands of sugarcane. Besides,the treatment III sugarcane-specific base fertilizers,containing certain amount of organic matters,could promote the sugar accumulation of sugarcane. Therefore,the sugarcane yield of treatment III was higher than that of treatment II. In conclusion,slow-release fertilizers and sugarcane-specific fertilizers can significantly increase sugarcane yield,especially sugarcane-specific fertilizers. Sugarcane-specific fertilizers have reasonable ratio and contain certain amount of organic matters,and can increase sugar content,obtain significant economic benefits,so it is worth popularization in large areas.

关键词： Slow-release fertilizer Sugarcane-specific fertilizer Sugarcane Growth Yield

Estimation of Production Amount of Livestock and Poultry Manure and Environmental Impact Assessment in Guangxi

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Asian Agricultural Research 2013年第11期5卷 93-96,100页

作者： Qing LIAO Dongliang HUANG Zepu JIANG Guangpo WEI panxia liang Yuning WANG Yangrui LI Agricultural Resource and Environment Research Institute Guangxi Academy of Agricultural Sciences Sugarcane Research Center Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement ( Guangxi) Ministry of Agriculture/Guangxi Key Laboratory of Sugarcane Genetic Improvement

This study was intended to estimate production of major livestock and poultry manure and contaminant content,and find out current situation of manure pollution,so as to provide reference for pollution control of livestock and poultry breeding industry in *** on the related statistic data in 2010 and the excretion coefficient of different livestock and poultry,the manure and its contaminant production amount of main livestock and poultry in Guangxi were *** the annual livestock and poultry manure load of farmland and the loss of contaminant were also calculated to analyze the ecological pressure resulted from livestock and poultry breeding in *** results were obtained:in 2010,the production amount of the livestock and poultry manure in Guangxi was 9141.30×104tons,including nutrient TN42.07×104tons and TP 13.62×104tons;the annual livestock and poultry manure and N,P pure nutrient load of farmland was 21t/hm2,98kg/hm2,and 32 kg/hm2respectively;the production amount of manure contaminants was BOD5383.43×104tons,COD Cr435.42×104tons,and NH3-N 42.08×104tons;according to 30%loss rate,the loss amount of COD Cr and NH3-N was higher than the sum of industrial and life waste *** was concluded that the livestock and poultry breeding industry had little impact on soil environment,but posed a grave threat to water environment.

关键词： Livestock and poultry manure Pollution Environment

Form and Interconversion Factor of Selenium in Soil

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Agricultural Biotechnology 2019年第1期8卷 116-120页

作者： Ying XING Yongxian LIU panxia liang Qing LIAO Liping PAN Taiqing HUANG Zepu JIANG Agricultural Resources and Environment Institute Guangxi Academy of Agricultural Sciences

In recent years,production and processing of natural selenium-rich agricultural products has become a research hotspot. The main factor affecting the selenium content in plant is the content of effective selenium in soil. So it is important to understand the form and conversion relationship of selenium in soil and find ways to improve selenium effectiveness. By summarizing the researches on selenium at home and abroad,we analyze the classification of soil selenium form,the transformation among different selenium forms and the improving factors of selenium effectiveness. We understand the relationship between chemical form and extraction form of selenium,analyze the driving factors of conversion among different forms,and discuss the ways to improve selenium effectiveness of soil in high selenium-rich area. By combining characteristics of soil and crop in different selenium-rich regions,different selenium activation methods are used,which could provide more scientific evidence for further selenium research.

关键词： Soil Selenium Form Conversion factor

Optimization of Extraction Process of Anthocyanins from Selenium-enriched Purple Potato by Response Surface Methodology

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Agricultural Biotechnology 2019年第4期8卷 173-177页

作者： Deming LIU Yongxian LIU Dongmei WANG panxia liang Liping PAN Jinping CHEN Jinhuan QIN Analysis and Testing Center Hunan Agricultural UniversityChangsha 410128China Agricultural Resource and Environment Research Institute Guangxi Academy of Agricultural Sciences/Guangxi Selenium Rich Agricultural Research CenterNanning 530000China Nanning Institute of Agricultural Sciences Nanning 530021China

Ultrasound-assisted extraction method was applied to extract the anthocyanins from selenium-enriched purple potato,and the optimum extraction conditions for anthocyanins in selenium-enriched purple potato were obtained by response surface methodology:the phosphoric acid concentration of 1.0%,the solid-liquid ratio at 5,and the extraction time of 19 *** these conditions,the yield of anthocyanins in selenium-enriched purple potato reached(0.409±0.010)mg/g(RSD=2.51%,n=3).The extraction of anthocyanins from selenium-rich fresh purple potato with dilute phosphoric acid has the advantages of less acid consumption and good extraction effect.

关键词： Selenium Purple potato Anthocyanin Extraction

Study on Extraction Method of Selenium-enriched Rice

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Agricultural Biotechnology 2018年第3期7卷 165-169页

作者： panxia liang Xiu LAN Yongxian LIU Liping PAN Mengling NONG Ying XING Qing LIAO Shiyang LU Jinping CHEN Resources and Environment Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 China Guangxi University Nanning 530007China

[Objectives] The aims were to optimize the extraction process of selenoproteins from selenium-enriched rice in Guangxi and provide references for the intensive processing and comprehensive utilization of selenium protein resources. [Methods]Selenium-enriched rice was used as materials to extract selenoproteins by phosphate buffer extraction method and to optimize the extraction process of selenoproteins by using the orthogonal experiment. Proteins and selenium content was measured by Coomassie Brilliant Blue G-250 reagent and AFS（ atomic fluorescence spectrometry） respectively. [Results] The most significant factor affecting extraction of rice Selenoproteins was extraction NaO H concentration,followed by the ratio of solid-liquid,temperature and then extraction time. The optimum extraction conditions of selenoproteins from rice were extraction temperature of 50 ℃,NaO H concentration of 0. 14 mol/L,extraction time of 5 h,and solid-liquid ratio of 1∶ 30. [Conclusions]The alkali extraction process optimized by orthogonal test could effectively improve the extraction rate of selenoproteins,and the optimized process parameters could be popularized and applied in practical production.

关键词： Selenium-enriched rice Selenoproteins Orthogonal experiment Extraction efficiency

High-quality Development of Selenium-enriched Industry in Guangxi Driven by Scientific and Technological Innovation

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Asian Agricultural Research 2020年第10期12卷 4-6页

作者： Yongxian LIU Ying XING Xue TANG Zhong HE Liping PAN Jinping CHEN Boqian CHEN Qi liang Jingmao XU Zepu JIANG panxia liang Qing LIAO Taiqing HUANG Institute of Agricultural Resource and Environment Guangxi Academy of Agricultural Sciences/Guangxi Selenium-enriched Agricultural Products Engineering Technology Research CenterNanning 530007China Guangxi University Nanning 530005China Guangxi Green Food Group Co. Ltd.Nanning 530022China Guangxi Selenium-enriched Agricultural Research Center Nanning 530007China

As the largest natural selenium-enriched soil area in China,Guangxi has achieved a leapfrog development in its selenium-enriched industry through the implementation of major scientific and technological projects driven by selenium-enriched *** selenium-enriched industry has also become a new force in the poverty alleviation industry in Guangxi,making remarkable *** summarizing and sorting out the effective measures and effects of scientific and technological innovation driving the development of selenium-enriched industry,the present paper analyzed some shortcomings and problems in the high-quality development of the selenium-enriched industry driven by the scientific and technological ***,in view of the problems,it came up with pertinent recommendations and countermeasures,to better promote high-quality development of the selenium-enriched industry and boost the rural revitalization.

关键词： Scientific and technological innovation Selenium enrichment High-quality development Rural revitalization

Current Development and Prospects of Selenium-enriched Moringa oleifera Industry in Guangxi

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Asian Agricultural Research 2018年第6期10卷 14-17页

作者： panxia liang Yongxian LIU Guoxin SHA Liping PAN Ying XING Qing LIAO Zepu JIANG Xiu LAN Jinping CHEN Shiyang LU Agricultural Resource and Environmnent Research Institute Guangxi Academy of Agricultural Sciences Nanning 53007 China Guangxi Xiyuan Moringa Co. Ltd. Nanning 530007 China Guangxi University Nanning 530007 China

Due to its unique economic value,Moringa oleifera Lam. has become a tree species with great development potential. This paper firstly introduced the value of development and utilization of M. oleifera in nutrition,medicinal,industrial,greening,etc. Then,focusing on the current development status of M. oleifera industry in Guangxi and combining Guangxi's resource conditions,it came up with recommendations for the comprehensive development and rational utilization of M. oleifera. Besides,it made a prediction of the prospect of Se-enriched *** industry in Guangxi.

关键词： Moringa oleifera Lam. Selenium Current development Prospects Guangxi

甘蔗核苷二磷酸激酶(NDPK1)基因克隆及表达分析

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热带作物学报 2012年第12期33卷 2199-2205页

作者：梁潘霞李杨瑞杨丽涛广西大学亚热带农业生物资源保护与利用国家重点实验室广西南宁530004 广西作物遗传改良生物技术重点开放实验室广西南宁530007 广西农业科学院资源与环境研究所广西南宁530007 中国农业科学院甘蔗研究中心/农业部广西甘蔗生物技术与遗传改良重点实验室/广西甘蔗遗传改良重点实验室广西南宁530007

采用RT-PCR技术克隆了甘蔗核苷二磷酸激酶基因全长cDNA,并用荧光定量PCR分析该基因的表达情况。结果表明:克隆得到的cDNA片段长度为686 bp,包括1个450 bp的开放阅读框,编码149个氨基酸,GenBank登录号为JQ712580。甘蔗与高粱NDPK基因cDN... 详细信息

采用RT-PCR技术克隆了甘蔗核苷二磷酸激酶基因全长cDNA,并用荧光定量PCR分析该基因的表达情况。结果表明:克隆得到的cDNA片段长度为686 bp,包括1个450 bp的开放阅读框,编码149个氨基酸,GenBank登录号为JQ712580。甘蔗与高粱NDPK基因cDNA序列的同源性为96%,氨基酸序列同源性为98%;该基因推导的蛋白分子量大小为16.83 ku,等电点为6.58,疏水性分值在-2.089～2.033;蛋白二级结构中α-螺旋占44.3%,随机卷曲占24.83%,延伸链占20.13%,β-转角只占10.74%。实时荧光定量分析结果表明,随着甘蔗干旱胁迫时间的延长,NDPK1基因表达均呈先升高后降低的趋势,30 h达最大;在处理后的18、30、40 h,PEG与PEG+Si处理的NDPK1基因的表达量有显著差异。

关键词：甘蔗核苷二磷酸激酶克隆生物信息学分析表达