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Midgut bacterial communities in the giant Asian honeybee （Apis dorsata） across 4 developmental stages： A comparative study

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Insect Science 2017年第1期24卷 81-92页

作者： Prakaimuk Saraithong Yihong Li Kanokporn Saenphet Zhou Chen panuwan chantawannakul Department of Biology Faculty of Science Chiang Mai University Chiang Mai 50200 Thailand Department of Basic Science and Craniofacial Biology New York University College of Dentistry NY 10010 USA Materials Science Research Center Faculty of Science Chiang Mai University Chiang Mai 50200 Thailand

Bacterial communities are known to play important roles during the developmental stages of insects, but current knowledge of bacteria associated with the midgut of Apis dorsata, the giant Asian honeybee, is limited. Using polymerase chain reaction-denaturing gradient gel electrophoresis analysis （PCR-DGGE） and 16S rRNA sequencing, the aim of this study was to determine the dynamics of bacterial community structure across four A. dorsata life stages in different geographical locations. The results reveal that bacterial diversity increased as the bee progressed through larval stage to newly emerged worker and old worker. However, in the pupal stage, no bands identified as bacteria could be observed. Overall, 2 bacterial phyla （Proteobacteria and Firmicutes） and 4 classes （Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, and Bacilli） were identified, but the frequency varied among the different stages and locations. The classes of Gammaproteobacteria and Bacilli dominated among larval, newly emerged worker and old worker developmental stages.

关键词： Apis dorsata DGGE gut flora insect bacteria microbial diversity 16S rRNA gene

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Comparative susceptibility and immune responses of Asian and European honey bees to the American foulbrood pathogen, Paenibacillus larvae

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Insect Science 2019年第5期26卷 831-842页

作者： Sasiprapa Krongdang Jay D.Evans Yanping Chen Wannapha Mookhploy panuwan chantawannakul 1Bee Protection Laboratory Department of BiologyFaculty of ScienceChiang Mai UniversityChiang MaiThailand Interdisciplinary Program in Biotechnology Graduate SchoolChiang Mai UniversityChiang MaiThailand USDA-ARS Bee Research LaboratoryBeltsvilleMarylandUSA Environmental Science Research Center Faculty of ScienceChiang Mai UniversityChiang MaiThailand

American foulbrood (AFB) disease is caused by Paenibacillus larvae. Currently, this pathogen is widespread in the European honey bee— Apis mellifera. However, little is known about infectivity and pathogenicity of P. lan'ae in the Asiatic cavity-nesting honey bees, Apis cerana. Moreover, comparative knowledge of P. larvae infectivity and pathogenicity between both honey bee species is scarce. In this study, we examined susceptibility, larval mortality, survival rate and expression of genes encoding antimicrobial peptides (AMPs) including defensin, apidaecin, abaecin, and hymenoptaecin in A. mellifera and A. cerana when infected with P. larvae. Our results showed similar effects of P. larvae on the survival rate and patterns of AMP gene expression in both honey bee species when bee larvae are infected with spores at the median lethal concentration (LC5 0 ) for A. mellifera. All AMPs of infected bee larvae showed significant upregulation compared with noninfected bee larvae in both honey bee species. However, larvae of A. cerana were more susceptible than A. mellifera when the same larval ages and spore concentration of P. larvae were used. It also appears that A. cerana showed higher levels of AMP expression than A. mellifera. This research provides the first evidence of survival rate, LC50 and immune response profiles of Asian honey bees, A. cerana, when infected by P. larvae in comparison with the European honey bee, A. mellifera.

关键词： Apis cerana Apis mellifera honey bee immune gene Paenibacillus larvae susceptibility

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A novel ascosporogenous yeast species,Zygosaccharomyces siamensis,and the sugar tolerant yeasts associated with raw honey collected in Thailand

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Fungal Diversity 2012年第1期52卷 123-139页

作者： Sujinan Saksinchai Motofumi Suzuki panuwan chantawannakul Moriya O.hkuma Saisamorn Lumyong The Graduate School Chiang Mai UniversityChiang Mai 50200Thailand Department of Biology Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Microbe Division/Japan Collection of Microorganisms(JCM) RIKEN BioResource CenterWakoSaitama 351-0198Japan

Diversity of yeasts in association with bees and their food sources has been explored during the last *** Thailand,there has been no study of yeast identification in honey and ***,a total of 186 yeast strains were isolated from 37 honey samples of 12 different bee *** the basis of morphological and physiological characteristics,55 representative strains were chosen and identified by sequence analysis of the 26S rDNA D1/D2 domain and the ITS *** data were compared with the published sequences and the results showed the occurrence of 19 ascomycetous and 1 basidiomycetous yeast *** strains of the new species were *** analysis of the 26S rDNA D1/D2 sequence revealed that they were conspecific and most closely related to Zygosaccharomyces *** on the ITS sequence,the new species was clustered with the type"and clearly distinguished from the type!.Sequence analysis of combined ITS-26S rDNA D1/D2 showed similar *** occurrence of these two types,with a divergence of more than 1%in their sequences,and low DNA relatedness among them suggested that members of the typeβcan be regarded as separate *** analysis of the morphological and physiological characteristics was *** formation was observed on acetate agar and Gorodkowa *** new Zygosaccharomyces species differed physiologically from *** in 4 assimilation *** data supports the hypothesis that the new species,Zygosaccharomyces siamensis,is a novel ascosporogenous *** type strain is JCM 16825T(=CBS 12273T)and a description is given here.

关键词： Ascosporogenous yeast Zygosaccharomyces siamensis Sugar tolerant yeasts Raw honey Thailand

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Social apoptosis in honey bee superorganisms

Social apoptosis in honey bee superorganisms

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二十一世纪第二届全国蜂业科技与蜂产业发展大会

作者：蔺哲广 Paul Page Ninat Buawangpong Huoqing Zheng Fuliang Hu Peter Neumann panuwan chantawannakul Vincent Dietemann College of Animal Sciences Zhejiang University Institute of Bee Health Vetsuisse FacultyUniversity of Bern Social Insect Research Group Department of Zoology and EntomologyUniversity of Pretoria

蜜蜂作为一种典型的真社会性昆虫可以被视作超级生物体,蜂群内世代层叠、协同合作的个体组成了一个功能上类似于多细胞生物的有机体,这种协作配合可通过社会性免疫机制限制群体内致病原的传播。然而,当蜂群面对一种新兴的疾病时,已有的... 详细信息

蜜蜂作为一种典型的真社会性昆虫可以被视作超级生物体,蜂群内世代层叠、协同合作的个体组成了一个功能上类似于多细胞生物的有机体,这种协作配合可通过社会性免疫机制限制群体内致病原的传播。然而,当蜂群面对一种新兴的疾病时,已有的社会性免疫便突显出不足,如蜜蜂的体外寄生虫狄斯瓦螨Varroa destructor,从其原始寄主东方蜜蜂Apis cerana迁移至西方蜜蜂Apis mellifera,目前已成为全球养蜂业的最大威胁。本研究首次揭示了发育中的东方蜜蜂工蜂幼虫较西方蜜蜂更为脆弱、敏感的生理特性致使其在被狄斯瓦螨寄生后更易死亡,从而有效限制了瓦螨的繁殖与传播。这种通过牺牲部分患病个体以避免疾病在群体中传播,从而保全整体健康的现象很可能是群居生物抵抗疾病的一种基本防御策略。社会性昆虫跨世代（transgenerational）群体免疫的新机制——类细胞凋亡免疫的提出,增进了当前对社会性昆虫生物学的了解,提升了对狄斯瓦螨与其原始寄主间互作关系的认识,且具有颇高的蜂群抗病育种的应用价值。

关键词：真社会性昆虫社会性免疫东方蜜蜂西方蜜蜂狄斯瓦螨

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泰国北部地区与蜜蜂相关的放线菌的多样性

泰国北部地区与蜜蜂相关的放线菌的多样性

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第九届亚洲养蜂大会

作者： Yaowanoot Promnuan Takuji Kudo Wasu Pathom-aree panuwan chantawannakul Department of Biology Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Japan Collection of Microorganisms RIKEN BioResource CenterWakoSaitama 351-0198Japan

＜正＞放线菌是产生生物活性物质（尤其是抗生素）的主要微生物群类之一。尽管蜜蜂中常检测出有细菌、酵母菌和霉菌等微生物,但从未报道有放线菌检出。本文旨在研究泰国地区蜂箱中放线菌的多样性。收集泰国北部地区的蜂具及子脾样品,以... 详细信息

＜正＞放线菌是产生生物活性物质（尤其是抗生素）的主要微生物群类之一。尽管蜜蜂中常检测出有细菌、酵母菌和霉菌等微生物,但从未报道有放线菌检出。本文旨在研究泰国地区蜂箱中放线菌的多样性。收集泰国北部地区的蜂具及子脾样品,以添加了抗生素的淀粉-酪蛋白琼脂分离放线菌。总共分离出了9个放线菌分离菌,通过形态学、生理学、化学及分子特征等对每个分离菌进行鉴定。结果发

关键词：放线菌蜜蜂微生物群细菌多样性

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