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An artificial chromosome for data storage

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National Science Review 2021年第5期8卷 67-75页

作者： Weigang Chen Mingzhe Han Jianting Zhou Qi Ge panpan Wang Xinchen Zhang Siyu zhu Lifu Song Yingjin Yuan School of Microelectronics Tianjin University Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education) Tianjin University Syn Bio Research Platform Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)School of Chemical Engineering and TechnologyTianjin University

DNA digital storage provides an alternative for information storage with high density and long-term ***, we report the de novo design and synthesis of an artificial chromosome that encodes two pictures and a video clip. The encoding paradigm utilizing the superposition of sparsified error correction codewords and pseudo-random sequences tolerates base insertions/deletions and is well suited to error-prone nanopore sequencing for data retrieval. The entire 254 kb sequence was 95.27% occupied by encoded *** Transformation-Associated Recombination method was used in the construction of this chromosome from DNA fragments and necessary autonomous replication sequences. The stability was demonstrated by transmitting the data-carrying chromosome to the 100 th generation. This study demonstrates a data storage method using encoded artificial chromosomes via in vivo assembly for write-once and stable replication for multiple retrievals, similar to a compact disc, with potential in economically massive data distribution.

关键词： DNA storage synthetic biology indel correction encoded DNA artificial chromosome

RcSPL1-RcTAF15b regulates the flowering time of rose (Rosa chinensis)

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Horticulture Research 2023年第6期10卷 182-194页

作者： Rui Yu Zhiying Xiong Xinhui zhu panpan Feng Ziyi Hu Rongxiang Fang Yuman Zhang Qinglin Liu Department of Ornamental Horticulture College of HorticultureChina Agricultural UniversityBeijing 100193China National Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of Sciencesand National Plant Gene Research CenterBeijing 100101China University of Chinese Academy of Sciences Beijing 101408China

Rose(Rosa chinensis),which is an economically valuable floral species worldwide,has three types,namely once-flowering(OF),occasional or re-blooming(OR),and recurrent or continuous flowering(CF).However,the mechanism underlying the effect of the age pathway on the duration of the CF or OF juvenile phase is largely *** this study,we observed that the RcSPL1 transcript levels were substantially upregulated during the floral development period in CF and OF ***,accumulation of RcSPL1 protein was controlled by *** ectopic expression of RcSPL1 in Arabidopsis thaliana accelerated the vegetative phase transition and ***,the transient overexpression of RcSPL1 in rose plants accelerated flowering,whereas silencing of RcSPL1 had the opposite ***,the transcription levels of floral meristem identity genes(APETALA1,FRUITFULL,and LEAFY)were significantly affected by the changes in RcSPL1 ***15b protein,which is an autonomous pathway protein,was revealed to interact with *** silencing and overexpression of RcTAF15b in rose plants led to delayed and accelerated flowering,***,the study findings imply that RcSPL1–RcTAF15b modulates the flowering time of rose plants.

关键词： TAF flowering floral

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桂北柑橘园土壤化学性状研究

土壤 2020年第6期52卷 1187-1195页

作者：张超博邱洁雅王敏李有芳朱攀攀王彤邓崇岭付行政凌丽俐刘升球陈传武彭良志西南大学柑桔研究所重庆400712 广西特色作物研究院广西桂林541004 四川省内江市农业农村局四川内江641000

为了解桂北柑橘园土壤化学状况,2017年在桂北7个柑橘主产县(市)选取102个代表性柑橘园采集土壤样本,对pH、有机质和10种养分进行定量分析。结果表明:土壤pH变幅3.95~8.02。其中,pH处于强酸性(pH7.3)范围的果园分别占总样本数的24.51%、4... 详细信息

为了解桂北柑橘园土壤化学状况,2017年在桂北7个柑橘主产县(市)选取102个代表性柑橘园采集土壤样本,对pH、有机质和10种养分进行定量分析。结果表明:土壤pH变幅3.95~8.02。其中,pH处于强酸性(pH7.3)范围的果园分别占总样本数的24.51%、48.04%和9.80%,大部分果园土壤pH为强酸性和酸性,有64.70%的果园土壤pH适宜或基本适宜(pH 4.5~7.0)柑橘生长,但仅有14.71%的果园土壤pH适宜柑橘生长(pH 5.5~6.5)。土壤有机质含量丰富,适量及以上水平的比例达86.27%。果园土壤营养元素丰缺并存,失衡比较明显,其中,有效磷和有效钾含量不足(低量或缺乏)的比例分别为33.33%和30.39%,超标(高量或过量)比例分别为21.57%和23.53%;碱解氮和有效钙含量主要在适量范围,不足比例分别为36.27%和31.37%;有效铁和有效锰含量丰富,超标比例分别为64.71%和55.89%;有效镁、锌、铜和硼含量不足比例较高,分别为59.80%、92.16%、56.86%和50.98%。桂北柑橘产区土壤养分限制因子主要为土壤酸化,有效镁、锌、铜和硼含量不足,生产上应注重调节土壤酸碱度,适度增施镁、锌、铜和硼肥。

关键词：桂北柑橘园土壤养分

含钙镁硅硝基复合肥对纽荷尔脐橙增产提质效应的研究

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肥料与健康 2023年第4期50卷 33-37页

作者：马彦平周忠雄杨国富刘哲王晓明朱盼盼上海化工研究院有限公司上海200062 湖北凯龙楚兴化工集团有限公司湖北荆门448000 农业农村部长江中下游耕地保育重点实验室/华中农业大学微量元素研究中心湖北武汉430070

为了探究含钙镁硅硝基复合肥对赣南脐橙产量及品质的影响,以纽荷尔脐橙为对象,开展了田间小区试验。试验共设3个处理,分别为不施肥(T1)、习惯施肥(T2)和施用含钙镁硅硝基复合肥(T3)。结果表明:T3处理可提高脐橙的挂果数、单果质量及果... 详细信息

为了探究含钙镁硅硝基复合肥对赣南脐橙产量及品质的影响,以纽荷尔脐橙为对象,开展了田间小区试验。试验共设3个处理,分别为不施肥(T1)、习惯施肥(T2)和施用含钙镁硅硝基复合肥(T3)。结果表明:T3处理可提高脐橙的挂果数、单果质量及果实横径,其中T3处理的挂果数比T2处理的显著提高15.62%,果实横径属于优质果范畴;T3处理还可改善果实品质,其中可溶性固形物含量比T2处理的显著提高15.50%,维生素C含量显著提高15.90%,可滴定酸含量显著降低39.09%,糖酸比、固酸比和可食率都有一定程度的提升。相较于习惯施肥,施用含钙镁硅硝基复合肥能提高脐橙的产量和品质。

关键词：含钙镁硅硝基复合肥脐橙品质产量

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干旱胁迫对小麦颖果发育的影响

扬州大学学报（农业与生命科学版） 2017年第1期38卷 81-88页

作者：陈昕钰杨阳盛洁悦聂甲玥汪巧菊张小惠朱盼盼杨蓉张二金余徐润熊飞扬州大学生物科学与技术学院江苏扬州225009

以扬麦16为材料,在小麦幼苗返青期至颖果成熟期内进行干旱处理,研究小麦颖果组织在干旱胁迫下细胞学结构的变化特征。结果表明:(1)在颖果发育过程中,干旱胁迫能促进前期颖果生长,但抑制后期颖果生长。(2)干旱胁迫在颖果发育早期促进果... 详细信息

以扬麦16为材料,在小麦幼苗返青期至颖果成熟期内进行干旱处理,研究小麦颖果组织在干旱胁迫下细胞学结构的变化特征。结果表明:(1)在颖果发育过程中,干旱胁迫能促进前期颖果生长,但抑制后期颖果生长。(2)干旱胁迫在颖果发育早期促进果皮组织发育,而在后期阻碍果皮细胞的程序性死亡,降低果皮中淀粉体的降解速率。(3)干旱胁迫下颖果胚乳组织在早期发育较快,但在后期胚乳细胞的进一步生长受到抑制,胚乳淀粉体的积累减少。(4)干旱胁迫下小麦颖果在发育早期胚组织分化早、发育快,但随着干旱的持续,胚体积的进一步扩张受阻,营养物质积累下降。综合而言,干旱胁迫通过改变颖果各组织结构发育影响颖果生长发育,总体表现为在发育前期促进颖果生长,此后随着干旱的持续,阻碍颖果的进一步发育和成熟。

关键词：小麦干旱胁迫颖果发育

Molecular engineering tuning optoelectronic properties of thieno[3,2-b]thiophenes-based electrochromic polymers

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Science China Chemistry 2017年第1期60卷 63-76页

作者： Mengmeng zhu Weishuo Li panpan Xu Jingjing Shi Shan Shao Xiaosi zhu Yitong Guo Yaowu He Zhao Hu Hongtao Yu Yanan zhu Igor F.Perepichka Hong Meng School of Advanced Materials Peking University Shenzhen Graduate School School of Chemistry Bangor University

Thieno[3,2-b]thiophene(TT)monomers end-capped with 3,4-ethylenedioxythiophene(EDOT)moieties are electropolymerized to formπ-conjugated polymers with distinct electrochromic(EC)*** and electronic factors(electron donor and acceptor substituents)in the side groups of the TT core,as well as the structure of the polymer backbone strongly affect the electrochemical and optical properties of the polymers and their electrochromic *** studied polymers show low oxidation potentials,tunable from-0.78 to+0.30 V(***/Fc^+)and the band gaps from 1.46 to 1.92 eV and demonstrate wide variety of color palettes in polymer films in different states,finely tunable by structural variations in the polymer backbone and the side *** materials of different colors in their doped/dedoped states have been developed(violet,deep blue,light blue,green,brown,purple-red,pinkish-red,orange-red,light gray,cyan and colorless transparent).High optical contrast(up to 79%),short response time(0.57-0.80 s),good cycling stability(up to 91%at 2000 cycles)and high coloration efficiency(up to 234.6 cm2C^(-1))have been demonstrated and the influence of different factors on the above parameters of EC polymers have been discussed.

关键词： electrochromic conducting polymer electrochemistry thieno[3 2-b]thiophene EDOT

Increased leptin by hypoxic-preconditioning promotes autophagy of mesenchymal stem cells and protects them from apoptosis

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Science China(Life Sciences) 2014年第2期57卷 171-180页

作者： WANG LiHan HU XinYang zhu Wei JIANG Zhi ZHOU Yu CHEN panpan WANG JianAn Cardiovascular Key Lab of Zhejiang Province the Second Affiliated Hospital School of Medicine Zhejiang University

Autophagy is the basic catabolic progress involved in cell degradation of unnecessary or dysfunctional cellular *** has been proven that autophagy could be utilized for cell survival under ***-preconditioning(HPC)could reduce apoptosis induced by ischemia and hypoxia/serum deprivation(H/SD)in bone marrow-derived mesenchymal stem cells(BMSCs).Previous studies have shown that both leptin signaling and autophagy activation were involved in the protection against apoptosis induced by various stress,including ***,it has never been fully understood how leptin was involved in the protective effects conferred by *** the present study,we demonstrated that HPC can induce autophagy in BMSCs by increased LC3-II/LC3-I ratio and autophagosome ***,similar effects were also observed when BMSCs were pretreated with *** beneficial effects offered by HPC were absent when BMSCs were incubated with autophagy inhibitor,3-methyladenine(3-MA).In addition,down-regulated leptin expression by leptin-shRNA also attenuated HPC-induced autophagy in BMSCs,which in turn was associated with increased apoptosis after exposed to sustained H/***,increased AMP-activated protein kinase phosphorylation and decreased mammalian target of rapamycin phosphorylation that were observed in HPC-treated BMSCs can also be attenuated by down-regulation of leptin *** data suggests that leptin has impact on HPC-induced autophagy in BMSCs which confers protection against apoptosis under H/SD,possibly through modulating both AMPK and mTOR pathway.

关键词： BMSCs autophagy hypoxic-preconditioning leptin apoptosis

Electrospinning-hot pressing technique for the fabrication of thermal and electrical storage membranes and its applications

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International Journal of Minerals,Metallurgy and Materials 2024年第9期31卷 1945-1964页

作者： panpan Che Baoshan Xie Penghui Cao Youfu Lv Daifei Liu Huali zhu Xianwen Wu Zhangxing He Jian Chen Chuanchang Li Key Laboratory of Renewable Energy Electric-Technology of Hunan Province School of Energy and Power EngineeringChangsha University of Science and TechnologyChangsha 410114China State Key Laboratory of Disaster Prevention&Reduction for Power Grid Changsha University of Science and TechnologyChangsha 410114China School of Chemistry and Chemical Engineering Jishou UniversityJishou 416000China School of Chemical Engineering North China University of Science and TechnologyTangshan 063009China

The combination of electrospinning and hot pressing,namely the electrospinning-hot pressing technique(EHPT),is an efficient and convenient method for preparing nanofibrous composite materials with good energy storage *** emerging composite membrane prepared by EHPT,which exhibits the advantages of large surface area,controllable morphology,and compact structure,has attracted immense *** this paper,the conduction mechanism of composite membranes in thermal and electrical energy storage and the performance enhancement method based on the fabrication process of EHPT are systematically ***,the state-of-the-art applications of composite membranes in these two fields are *** particular,in the field of thermal energy storage,EHPT-prepared membranes have longitudinal and transverse nanofibers,which generate unique thermal conductivity pathways;also,these nanofibers offer enough space for the filling of functional ***,EHPT-prepared membranes are beneficial in thermal management systems,building energy conservation,and electrical energy storage,e.g.,improving the electrochemical properties of the separators as well as their mechanical and thermal *** application of electrospinning-hot pressing membranes on capacitors,lithium-ion batteries(LIBs),fuel cells,sodium-ion batteries(SIBs),and hydrogen bromine flow batteries(HBFBs)still requires *** the future,EHPT is expected to make the field more exciting through its own technological breakthroughs or be combined with other technologies to produce intelligent materials.

关键词： electrospinning-hot pressing technique thermal storage electrical storage composite membranes nanofiber

Unique fluorophilic pores engineering within porous aromatic frameworks for trace perfluorooctanoic acid removal

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National Science Review 2023年第10期10卷 106-113页

作者： Chi Zhang Junchao Dong panpan Zhang Lei Sun Liu Yang Wenjian Wang Xiaoqin Zou Yunning Chen Qingkun Shang Danyang Feng Guangshan zhu Faculty of Chemistry Northeast Normal University Institute of Molecular Sciences and Engineering Institute of Frontier and Interdisciplinary Science Shandong University

Perfluorooctanoic acid(PFOA),a representative of per/polyfluorinated alkyl substances,has become a persistent water pollutant of widespread concern due to its biological toxicity and refractory *** this work,we design and synthesize two porous aromatic frameworks(PAF) of PAF-CF3and PAF-C2F5using fluorine-containing alkyl based monomers in tetrahedral *** PAFs exhibit nanosized pores(～1.0 nm) of high surface areas(over 800 m2g-1) and good *** adsorption capacity(>740 mg g-1) and superior efficiency(>24 g mg-1h-1) are achieved toward the removal of PFOA with 1μg L-1concentration owing to unique C-F…F-C *** particular,PAF-CF3and PAF-C2F5are able to reduce the PFOA concentration in water to 37.9 ng L-1and 43.3 ng L-1,below EPA regulations(70 ng L-1).The reusability and high efficiency give both PAFs a great potential for sewage treatment.

关键词： porous aromatic frameworks fluorophilicity perfluorooctanoic acid adsorption removal halogen interactions