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实用临床医药杂志 2020年第15期24卷 1-7页

作者： Tamoghna Ghosh pankaj krishna Prakash Singh Bisen 印度医药城Avantor性能材料公司研发诊断中心印度德拉敦248011 斋浦尔国立大学医学科学和研究中心研究所印度斋浦尔302017 瓜廖尔大学生物技术研究学院印度瓜廖尔474011

人和动物的基因组有很大部分由各种内源性病毒组成,它们快速复制和突变,并呈现出时间和空间的变化性,导致或者不导致疾病。但是人类对于其组成、复杂性和动态变化了解不足。迄今为止,高通量基因组学、转录组学和蛋白质组学研究对病毒组... 详细信息

人和动物的基因组有很大部分由各种内源性病毒组成,它们快速复制和突变,并呈现出时间和空间的变化性,导致或者不导致疾病。但是人类对于其组成、复杂性和动态变化了解不足。迄今为止,高通量基因组学、转录组学和蛋白质组学研究对病毒组-微生物组-人类行为三者之间的相互作用有了一些重要的发现,结果表明其相互作用极大地影响着人体的生理和内稳态。大量的内源性病毒为宿主提供了益处,也带来不良的免疫调节和继发感染。很多寄宿于人体的病毒来源于哺乳动物和鸟类的跨物种传播,这种传播往往导致致死性高的流行病。理解病毒分类以及病毒蛋白质组中负责与宿主病毒组相互作用的元素和因子会帮助我们制订应对未来病毒爆发的策略。本文对病毒宏基因组学研究中与人类健康和疾病相关的一些关键内容进行了综述。

关键词：动物基因组高通量基因组病毒蛋白质组学病毒基因组人类病毒组内稳态宿主

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Use of a Bioaugmented Organic Soil Amendment in Combination with Gypsum for Withania somnifera Growth on Sodic Soil

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Pedosphere 2016年第3期26卷 299-309页

作者： Manjul GUPTA pankaj Kumar SRIVASTAVA SHIKHA Abhishek NIRANJAN Shri krishna TEWARI CSIR National Botanical Research Institute Rana Pratap Marg Lucknow 226001 (India) School of Environmental Sciences Babasaheb Bhimrao Ambedkar University Vidya Vihar Rae Bareilly Road Lucknow 226025 (India)

Limited availability of organic matter is a problem to sustain crop growth on sodic soil. Organic soil amendments are a costeffective source of nutrients to enhance crop growth. A field study was conducted to evaluate the effect of an organic soil amendment bioaugmented with plant growth-promoting fungi(SF_(OA) ) in combination with gypsum on soil properties and growth and yield attributes of Withania somnifera, one of the most valuable crops of the traditional medicinal system in the world, on a sodic soil at the Aurawan Research Farm of CSIR-National Botanical Research Institute, Lucknow, India. The SF_(OA) used was prepared by pre-enriching farm waste vermicompost with plant growth-promoting fungi before mixing with pressmud and Azadirachta indica seed cake. The application of SF_(OA) at 10 Mg ha^(-1)after gypsum(25.0 Mg ha^(-1)) treatment significantly(P < 0.05) increased root length(by 96%) and biomass(by 125%) of Withania plants compared to the control without SF_(OA) and gypsum. Similarly, the highest withanolide contents were observed in leaves and roots of Withania plants under 10 Mg ha^(-1)SF_(OA) and gypsum. Combined application of SF_(OA) and gypsum also improved physical, chemical and enzymatic properties of the soil, with the soil bulk density decreasing by 25%, water-holding capacity increasing by 121%, total organic C increasing by 90%, p H decreasing by 17% and alkaline phosphatase, β-glucosidase, dehydrogenase and cellulase activities increasing by 54%, 128%, 81% and 96%, respectively, compared to those of the control. These showed that application of the SF_(OA) tested in this study might reclaim sodic soil and further support Withania cultivation and results were better when the SF_(OA) was applied after gypsum treatment.

关键词： medicinal crop plant growth-promoting fungi soil property soil reclamation vermicompost

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In vivo subsite classification and diagnosis of oral cancers using Raman spectroscopy

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Journal of Innovative Optical Health Sciences 2016年第5期9卷 57-70页

作者： Aditi Sahu Atul Deshmukh Arti R.Hole pankaj Chaturvedi CMurali krishna Chilakapati Laboratory ACTRECTMC KhargharNavi Mumbai 410210India Head and Neck Surgical Oncology Tata Memorial HospitalDr.E.Borges RoadParelMumbaiMaharashtra-400012India

Oral cancers suffer from poor disease free survival rates due to delayed ***,rapid,objective approaches as adjuncts to visual inspection can help in better management of oral *** spectrosoopy(RS)has shown potential in identification of oral premalignant and malignant conditions and also in the detection of early cancer changes like cancer field ffects(CFE)at buccal mucosa *** differences between different oral subsites have also been reported using *** this study,anatomical differences between subsites and their possible infuence on healthy vs pathological classification were evaluated on 85 oral cancer and 72 healthy *** were acquired from buccal mucosa,lip and tongue in healthy,contralateral(internal healthy control),premalignant and cancer conditions using fiber optic Raman *** spectra indicate predominance of lipids in healthy buccal mucosa,contribution of both lipids and proteins in lip while major dominance of protein in tongue *** healthy to tumor,changes in protein secondary structure,DNA and heme related features were *** component linear discriminant analysis(PC-LDA)followed by leave one out CrOoss-validation(LOOCV)was used for data *** gs indicate buccal mucosa and tongue are distinct entities,while lip misclassifies with both these ***,the diagnostic algorithm for individual subsites gave improved classification efficiencies with respect to the pooled subsites ***,as the pooled subsites model yielded 98%specifcity and 100%sensitivity,this model may be more useful for preliminary screening ***-scale validation studies are a pre requisite before envisaging future clinical applications.

关键词： Oral cancer screening in vivo Raman spectroscopy PC-LDA.

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Genetic Behaviour of Bacterial Wilt Resistance in Brinjal (Solanum melongena L.) in Tropics of Andaman and Nicobar Islands of India

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American Journal of Plant Sciences 2016年第2期7卷 333-338页

作者： Naresh Kumar Bainsla Shrawan Singh pankaj Kumar Singh krishna Kumar Awanindra Kumar Singh Raj Kumar Gautam Central Island Agricultural Research Institute Port Blair India Current Address: Division of Genetics Indian Agricultural Research Institute New Delhi India

Brinjal or eggplant (Solanum melongena L.) is severely affected by bacterial wilt caused by Ralstonia solanecearum in Andaman and Nicobar Islands. Resistant varieties are most suitable option to reduce crop losses from bacterial wilt but knowledge of resistance mechanism and its inheritance is important to develop resistant varieties. Further, majority of germplasm from mainland India showed susceptible reaction under Andaman conditions. Thus, the present study was done during 2010-2012 to understand the genetic behaviour of bacterial wilt resistance in brinjal “CARI-B-1” (R) × “Pusa Purple Long” (S) in hot humid tropical climate of Andaman Islands. For this, the population from single F1 fruit was advanced to F2 and F3 and recorded the reaction of segregating population in the sick plots. The results revealed that there is preponderance of recessive gene family wherein more than one gene acts in additive mode. Another cross between S. torvum (R) × Diglipur local collection (S) also showed the recessive gene action for resistance as observed in F2 generation. Besides, the study also advocated that mechanisms of resistance, escape with early resistance and progressive escape have been found to be operating in individuals of segregating population.

关键词： Egg Plant Ralstonia solanecearum Genetics Islands Resistance

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