检索结果-南通市图书馆

In-VitroEffect of Steroids on Melanoma Cell Growth—A Prelude to Melanoma Treatment?

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Cancer Therapy 2018年第4期9卷 338-350页

作者： pandurangan ramaraj Department of Biochemistry Kirksville College of Osteopathic Medicine A.T. Still University Kirksville USA

Skin is not only a target organ for various sex steroids and hormones, but also an endocrine organ, which produces sex steroids. It has been suggested by Nikolakis et al. that impairment in skin steroidogenesis may result in inflammatory or autoimmune or other skin disorders. Melanoma is one such skin disease or disorder, which is believed to be caused by UV rays. But, epidemiological, clinical, in-vivo and in-vitro studies suggested the involvement of steroids in the regulation of melanoma growth. However, these studies either did not identify the steroid involved or did not relate to the protective function of the steroid in menstruating females in melanoma, as reported by the clinical studies. In this context, our studies with mouse and human melanoma cell lines showed that female sex steroid progesterone not only inhibited melanoma cell growth, but also affected adhesion and migration functions. In addition, our studies also showed that the effect of progesterone was not a toxic or spurious, but a specific effect on melanoma cells. Hence, our in-vitro studies along with previous other studies subscribed to the idea proposed earlier by Slominski et al. that modulation of local steroids could be a new therapeutic approach for treatment of skin disease or disorder, melanoma.

关键词： Steroids in Skin Steroids Action on Melanoma Cells Progesterone Treatment Melanoma Cell Growth

In-VitroEffect of Sex Steroids on Mouse Melanoma (B16F10) Cell Growth

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CellBio 2014年第2期3卷 60-71页

作者： pandurangan ramaraj James L. Cox Department of Biochemistry Kirksville College of Osteopathic Medicine A.T. Still University of Health Sciences Kirksville Missouri USA

A battery of sex steroids were used to check their effect on mouse melanoma (B16F10) cell growth in-vitro. Progesterone and its synthetic receptor antagonist RU-486 showed maximum inhibition on in-vitro melanoma cell growth [1]. Further research work showed that the inhibition by progesterone was not a toxic, spurious or non-specific effect on mouse melanoma cell growth and the inhibition by progesterone was not mediated through progesterone receptor.

关键词： Sex Steroids B16 Mouse Melanoma MTT Growth Assay

In-VitroDetermination of Biological and Anabolic Functions of Weak Androgen Dehydroepiandrosterone (DHEA) Using a Variety of Cell Lines

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Endocrine and Metabolic Diseases 2015年第8期5卷 105-116页

作者： James L. Cox Yingzi Chang pandurangan ramaraj Department of Biochemistry Kirksville College of Osteopathic Medicine A.T. Still University of Health Sciences Kirksville MO USA Department of Pharmacology Kirksville College of Osteopathic Medicine A. T. Still University of Health Sciences Kirksville MO USA

Dehydroepiandrosterone (DHEA) is a weak androgen and is shown to have anti-cancer, anti-atherogenic, anti-adipogenic and anti-inflammatory effects on mouse, rat and rabbit models. However, human clinical trials data did not support animal findings and were inconclusive. These systemic differences in biological actions between rodents and humans were attributed to the low level of DHEA in rodents. In order to further understand the differences in biological functions between rodents and humans, we resorted to an in-vitroapproach involving mouse, rat and human cell lines to assess DHEA biological and anabolic functions separately and independently without systemic influence. Results indicated that DHEA was effective on mouse and rat cell lines but not on human cell lines, as observed in in-vivo studies. In addition, our in-vitrostudy showed that DHEA was able to induce myogenesis in mouse mesenchymal cells revealing its anabolic function, even though DHEA was considered as a weak androgen. This observation lent credence to the ban on DHEA by IOC medical commission, citing DHEA as an anabolic steroid. These in-vitro experiments suggested that the differences in biological actions of DHEA between rodents and humans existed not only in-vivo at the systemic level, but also in-vitro at the cellular level and thus paving the way to study the mechanism responsible for these differences at the cellular level itself.

关键词： Dehydroepiandrosterone Various Cell Lines Biological Functions Anabolic Function

Effect of Androstenedione on Adipogenesis in Murine C3H10T1/2 Mesenchymal Cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Endocrine and Metabolic Diseases 2015年第2期5卷 9-18页

作者： pandurangan ramaraj Jorge N. Artaza Indrani Sinha-Hikim Wayne E. Taylor Department of Biochemistry Kirksville College of Osteopathic Medicine A.T. Still University of Health Sciences Kirksville MO USA Division of Endocrinology Charles R. Drew University of Medicine and Science Los Angeles CA USA Department of Health and Life Sciences Charles R. Drew University of Medicine and Science Los Angeles CA USA

Clinical trials of weak androgen androstenedione (AD) administered at a high concentration, showed an increase in muscle mass in men like strong androgens testosterone (T) and dihydrotestosterone (DHT), but did not show any inhibitory effect on fat mass unlike strong androgens. This observation prompted us to check the in-vitro effect of AD on adipogenesis using mouse mesenchymal multipotent cells (C3H10T1/2), which can differentiate into both myoblasts and adipocytes. Results indicated that AD inhibited adipogenesis at 10 nM, 100 nM and 1 μM concentrations, but not at 10 μM concentration. AD did not inhibit adipogenesis at 10 μM concentration and also did not inhibitmyogenesis at 10 μM concentration. Addition of bicalutamide, an androgen receptor (AR) antagonist decreased myogenesis and increased adipogenesis, indicating that the effect of AD was mediated through AR. Another weak androgen dehydroepiandrosterone (DHEA) also showed the same pattern of adipogenesis in 10T1/2 cells. AD also showed a similar pattern of adipogenesis in 3T3-L1 preadipocyte cells. Thus, the in-vitro results of AD on adipogenesis correlated with the in-vivo results of AD on fat-mass from clinical trials and suggested a possible difference in biological action between weak androgens (AD, DHEA) and strong androgens (T, DHT) on adipogenesis. Since the biological action of AD was mediated through AR, this physiological difference onadipogenesis could be due to the nature (partial agonist/antagonist) of AD binding to AR.

关键词： Androstenedione Adipogenesis Myogenesis Androgen Receptor Bicalutamide

Intensive-visible-light-responsive ANbO_(2)N(A = Sr, Ba) synthesized from layered perovskite A_(5)Nb_(4)O_(15) for enhanced photoelectrochemical water splitting

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2022年第5期31卷 529-537页

作者： Sukanya ramaraj Jeongsuk Seo Departmentof Chemistry.College of Natural Sciences Chonnam National University.77 Yongbong-ro Buk-gu Gwangju 61186Republicof Korea

Nb-based, perovskite-type oxynitrides ANb(O,N)_(3)(A = Sr, Ba) have been significantly attractive semiconductor materials for photoelectrochemical(PEC) water splitting due to their absorption abilities of intensive-visible-light. However, PEC activities of these perovskites are relatively low due to reduction of Nb species during nitridation leading to the generation of anion defects and impurity ***, we propose nitridation of A-rich, layered perovskite A_(5)Nb_(4)O_(15) as starting oxides for general synthesis of photoactive ANbO_(2)N. These layered perovskite A_(5)Nb_(4)O_(15) were completely transformed to single phase perovskite-type ANbO_(2)N by nitridation. Wavelength onsets of light absorption were observed at 700 nm for SrNbO_(2)N and at 740 nm for BaNbO_(2)N. According to X-ray photoelectron spectroscopy results,excess Lewis base A species significantly suppressed the generation of reduced species such as Nb^(4+) and Nb^(3+)during nitridation, although it led to an amorphous surface of the as-prepared *** annealing in Ar largely enhanced surface crystallinity of ANbO_(2)N. As a result, Co(OH)_(x)/ANbO_(2)N/FTO photoanodes, prepared by loading Co(OH)_(x) electrocatalyst, showed high photocurrent density of 1.6(A = Sr) and 2.4 m A cm^(-2)(A = Ba) at 1.23 VRHEunder AM 1.5 G simulated sunlight. These results demonstrate that A-rich layered perovskite A_(5)Nb_(4)O_(15)are effective starting precursors for preparing lowdefective ANbO_(2)N, thus improving PEC water splitting activity.

关键词： Perovskite-type oxynitride CaNbO_(2)N SrNbO_(2)N BaNbO_(2)N A-rich starting oxide Surface heat treatment Ar annealing Solar water oxidation

Biochar derived from non-customized matamba fruit shell as an adsorbent for wastewater treatment

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Bioresources and Bioproducts 2022年第2期7卷 27-33页

作者： Gotore Obey Munodawafa Adelaide Rameshprabu ramaraj Graduate School of Advanced Engineering Nagasaki UniversityNagasakiJapan Department of Land and Water Resources Management Midlands State UniversityGweruZimbabwe School of Renewable Energy Maejo UniversityChiang MaiThailand

This study of Matamba shell reviled them as material with outstanding surface morphology,el-emental and kinetic mechanism *** biochar revealed irregular honeycomb morphological transformation from the field emission scanning electron microscope after pyrol-ysis at 600℃ for 2 *** dispersive X-ray spectroscopy revealed high content of carbon(72.68wt%),nitrogen(14.14wt%)and oxygen(10.35wt%)on the biochar *** avail-able oxygen composition provides enough polarization ability for high iodine adsorption(43.65 mmol/g)from the experimental data which were significantly induced by weak van der Waals forces and𝜋-𝜋and𝜋-stacking interaction on the biochar surface and its *** carbon content above 50%in ash rich biochar with an increase in pyrolysis can be ascribed to elements incorporated into aromatic or heterocyclic ring system established through preferential loss of oxygen at 600℃ *** adsorption kinetics were conducted to evaluate the equilibrium adsorption of the novel material and Elovich and Intra particle diffusion better described well the kinetic adsorption through Iodine adsorption than pseudo first order and pseudo second or-der *** was the best model to fit the adsorption kinetics with 45.41 mmol/(g·min)adsorption *** second order Akaike Information Criterion(38.26),adjusted correlation co-efficient R 2(0.9898)and sum of squares error(1.442)were used to fit the ***,the biochar in this study can serve as a promising green material for efficiently removing organic and inorganic contaminants from the environmental water *** environmental signifi-cance of biochar will be of fundamental meaning to rural areas in developing countries in aquatic contaminants immobilization for water *** results indicate that the Matamba fruit shells has the possibility to be used as an eco-friendly and low-cost effective adsorbent for anionic dye removal from the water ***

关键词： Adsorption Mutamba-biochar Iodine-solution Kinetic-mechanism Nanomaterials

Multi-Objective Optimization with Artificial Neural Network Based Robust Paddy Yield Prediction Model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Intelligent Automation & Soft Computing 2023年第1期35卷 215-230页

作者： S.Muthukumaran P.Geetha E.ramaraj Department of Computer Science Alagappa UniversityKaraikudi630003India Department of Computer Science Dr.Umayal Ramanathan College for WomenKaraikudi630003India

Agriculture plays a vital role in the food production process that occupies nearly one-third of the total surface of the *** is propagated from the seeds of paddy and it is a stable food almost used byfifty percent of the total world *** extensive growth of the human population alarms us to ensure food security and the country should take proper food steps to improve the yield of food *** paper concentrates on improving the yield of paddy by predicting the factors that influence the growth of paddy with the help of Evolutionary Computation *** of the researchers used to relay on historical records of meteorological parameters to predict the yield of *** is a lack in analyzing the day to day impact of meteorological parameters such as direction of wind,relative humidity,Instant Wind Speed in paddy *** real time meteorological data collected and analysis the impact of weather parameters from the day of paddy sowing to till the last day of paddy harvesting with regular time series.A Robust Optimized Artificial Neural Network(ROANN)Algorithm with Genetic Algorithm(GA)and Multi Objective Particle Swarm Optimization Algorithm(MOPSO)proposed to predict the factors that to be concentrated by farmers to improve the paddy yield in cultivation.A real time paddy data collected from farmers of Tamilnadu and the meteorological parameters were matched with the cropping pattern of the farmers to construct the *** input parameters were optimized either by using GA or MOPSO optimization algorithms to reconstruct the *** database optimized by using Artificial Neural Network Back Propagation *** reason for improving the growth of paddy was identified using the output of the Neural *** metrics such as Accuracy,Error Rate etc were used to measure the performance of the proposed *** analysis made between ANN with GA and ANN with MOPSO to identify the recommenda

关键词： ANN back propagation algorithm genetic algorithm multi objective particle swarm optimization algorithm

Design,simulation of different configurations and life-cycle cost analysis of solar photovoltaic-water-pumping system for agriculture applications:use cases and implementation issues

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Clean Energy 2022年第2期6卷 335-352页

作者： L.Ashok Kumar C.N.Lakshmiprasad G.ramaraj G.Sivasurya Department of Electrical and Electronics Engineering PSG College of TechnologyCoimbatoreIndia

Water is an essential resource for agriculture and the majority of land is irrigated through borewells or *** power requirement for an irrigation pump motor is fed by the on-grid power supply but the availability of electricity in rural areas is still *** rising concerns about global warming and the rise in carbon footprints,it is necessary to choose clean and green energy,thereby attaining self-sustainable *** receives yearly a mean solar irradiation of 6.5 kWh/m^(2)***,a solar photovoltaic-water-pumping system(SPV-WPS)is a suitable alternative to grid energy;thereby,the farmers would generate electricity through the solar photovoltaic system and become self-sufficient in their energy *** this paper,two different agricultural fields in Tamil Nadu,India that deploy flood irrigation and drip irrigation are taken as a case *** paper discusses the concerns on the use of grid power and their carbon footprint,design and simulation of 4-and 5.5-kW SPV-WPSs using PVsyst 7.1.1,and the advantages of using SPV-WPSs and life-cycle cost analysis on different use *** Government of India has introduced a special scheme to promote the installation of SPV-WPSs by offering attractive incentives through PM-Kisan Urja Suraksha evam Utthaan Mahabhiyan(KUSUM)*** results of the case study show that with the use of SPV-WPSs,either with or without subsidy,the farmer could gain a minimum of 250%on the investment with a project lifetime of 25 years.

关键词： solar water pump PVsyst PM-KUSUM subsidy life-cycle cost sustainable development goal

Studies on LH modulated 8 kDa peptide involved regulation of testosterone production in rat Leydig cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 1999年第4期1卷 191-194页

作者： P.ramaraj A.Jagannadha Rao Department of Diabetes Endocrinology & Metabolism The University of Hope National Medical Centre 15000 East Duarte Road Duarte CA 91007 USADepartment of Biochemistry & Department of Molecular Reproduction Development & Genetics Indian Institute of Science Bangalore-560 0112 India

Aim: To demonstrate the role of the 8 kDa peptide in regulation of testosterone production by mt Leydig cells. Meth-ods: A peptide similar to 8 kDa peptide purified from immature rat Leydig cells was isolated and purified from rat lungcytosol. Immunological and structural similarity between the peptides purified from lung and Leydig cells was estab-lished by Western blot and tryptic map comparison respectively. Results: Addition of the 8 kDa peptide 10, 50, 100;and 150 ug decreased the production of testosterone in Leydig cells dose-dependently. But the addition of the peptide150 ug along with hCG had no effect on hCG-stimulated increase in testosterone production. Conclusion: In vitro ad-dition of the peptide purified from lung cytosol to adult rat Leydig cells resulted in a concentration-dependent decrease inbasal testosterone production although it had no effect on hCG-stimulated testosterone production. (Asian J Androl1999 Dec; 1: 191-194)

关键词： Leydig cells testosterone regulation differentiation steroidogenesis peptides LH