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Germplasm Resources and Quality Research of Three Species of Chrysanthemums in Bozhou

Plant Diseases and Pests 2020年第5期11卷 38-40,45页

作者： Chen Qun Ma Shitang Yu Nianjun pan yuemin School of Pharmacy Bozhou Vocational and Technical CollegeBozhou 236800China Anhui Science and Technology University Hefei 233100China School of Pharmacy of Anhui University of Chinese Medicine Hefei 230036China School of Plant Protection of Anhui Agricultural University Hefei 230038China

[Objective]The paper was to preliminarily study the pharmacognosy of three species of chrysanthemums cultivated in Bozhou,and to compare their similarities and differences.[Method]Organs of three species of chrysanthemum samples were dissected and made into integral slices,and surface morphology of each part was observed *** slices were developed in the same way and observed under a *** to thin layer chromatography(TLC)test(Part I,Appendix VIB,2005 edition),2μL of sample solutions and reference solutions were absorbed respectively,and dripped on the same piece of silica gel G thin layer *** petroleum ether-acetone(2∶1)as the developing solvent,samples were developed and then *** were sprayed with chromogenic agent 2%aluminum trichloride,and examined under UV light at the wavelength of 365 *** fluorescent spots of the same color were displayed in the position corresponding to the chromatogram of reference solutions were observed.[Result]Non-glandular hairs were found in bract microstructures of three species of *** were many T-shaped hairs and few multicellular sessile non-glandular *** epidermal cell walls of bracts were parallel straight,and some were rectangular;stomas could be seen *** Dendranthema morifolium(Ramat.)Tzvel.‘Boju’*** Dendranthema morifolium(Ramat.)Tzvel.‘Da maya’*** fluorescent spots of the same color in the position corresponding to the chromatogram of reference sample acacetin in TLC test,and the spots of ***(Ramat.)Tzvel.‘Da maya’*** light in color;there were no spots observed in Chrysanthemum morifolium Ramat.[Conclusion]The external characters,internal forms and internal components of ***(Ramat.)Tzvel.‘Boju’***.,***(Ramat.)Tzvel.‘Da maya’*** *** Ramat are all related to germplasm resources of chrysanthemum *** contents of acacetin,one of the components of ***(Ra

关键词： Chrysanthemum Pharmacognosy Germplasm resources TLC Quality research

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Nitrate reductase required for sclerotial development and virulence formation in Sclerotinia sclerotiorum

Nitrate reductase required for sclerotial development and vi...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： pan yuemin Wei Junjun Jiang Bingxin 高智谋 College of Plant Protection Anhui Agricultural University

Nitrate reductase is a key enzyme in the process of nitrogen assimilation and is distributed in various organs and tissues of bacteria,fungi,and higher *** order to explore the function of this enzyme during the vegetative growth and pathogenesis of Sclerotinia sclerotiorum,the nitrate reductase-encoding gene of *** SsNR was cloned and the gene silencing technique was used to analyze the roles of SsNR in *** results showed that the growth rate of SsNR mutant colonies slowed down,the number of sclerotia was significantly reduced,the infection cushion was deformed,and the oxalic acid-producing ability was *** the influence of chemical stress factors,the transformants were highly sensitive to gongo red,sodium dodecyl sulfate,hydrogen peroxide,and sodium *** diameters of the colonies were significantly inhibited,and the transformants were only insensitive to *** inoculation test on the detached leaves of rapeseed showed that the lesion areas of wild-type strain accounted for 12.43%-13.66%of the entire leaf *** analysis of expression level of pathogenicity related genes showed that the expressions of Ggt1,Sac1,and Smk3 genes were downregulated and the expressions of Ubq and Cyp genes were *** above results indicated that SsNR played a critical role in the vegetative growth,sclerotia development,the formation of infection cushion,and pathogenesis of ***.

关键词： Sclerotinia sclerotiorum Nitrate reductase Sclerotium Pathogenicity

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Nitrate reductase is required for sclerotial development and virulence of Sclerotinia sclerotiorum

Nitrate reductase is required for sclerotial development and...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： pan yuemin Wei Junjun Yao Chuanchun Yang Guogen Gao Zhimou Department of Plant Pathology College of Plant ProtectionAnhui Agricultural University Anhui Academy of Agricultural Sciences

Sclerotinia sclerotiorum(Lib.) de Bary is a necrotrophic pathogen which causes disease on many crops including rapeseed,soybean,sunflower,peanut,and other important economic *** stem rot(SSR) is one of the most important diseases on rapeseed in China,which causes significant yield losses and economic damage threaten to oil ***,we characterized a nitrate reductase(SsNR) that play important roles in mycelia growth,sclerotia development and *** is highly expressed during sclerotial ***-silenced mutants showed abnormal in mycelia growth with short and branched tip,imperfect in sclerotia formation,and reduced virulence on rapeseed and soybean with abnormal in infection cushions formation and decreased in oxalic acid ***-silenced mutants are more sensitive to abiotic stress including Congo Red,SDS,HO and NaCl except *** expression level of pathogenicity related genes SsGgt1,SsSac1,and SsSmk3 are down-regulated while SsUbq and SsCyp are up-regulated in SsNR-silenced *** results indicate that SsNR is important for *** during growth and infection.

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Characterization and Evaluation of Bacillus pumilus BS-4 as a Novel Potential Biocontrol Agent against Phytophthora sojae in Soybean

Characterization and Evaluation of Bacillus pumilus BS-4 as ...

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中国植物病理学会2023年学术年会

作者： CAO Shun CHEN Fangxin DAI Yuli ZHAO Zhenyu JIANG Bingxin pan yuemin GAO Zhimou College of Plant Protection Anhui Agricultural University

Soybean Phytophthora root rot caused by Phytophthora sojae is one of the most devastating diseases of soybean *** study was conducted to develop new effective biocontrol agents for the control of the disease and explore the action *** antagonistic bacterial strains against *** were isolated from the soybean field soil samples, and among the strains, BS-4 not only had the strongest inhibitory activity against ***,but also had good inhibition to other four species of oomycetes in Phytophthora and eight fungal *** on 16 S rDNA sequence analysis and the physiological and biochemical properties, the strain BS-4 was identified as Bacillus *** BS-4 culture filtrate had significant inhibitory effects on the pathogens, and the inhibition rates were stronger than those in the dual ***-4 filtrate kept a high bioactivity to *** at a high temperature, even at 121℃, and in the pH range of 5 to *** germination of zoospores and the formation of sexual organs were seriously affected by BS-4 ***, the detached leaves and greenhouse experiments showed that BS-4 suspension and culture filtrate all had good control efficacy on PRR, and in the field experiment, BS-4 suspension reduced the disease index and increased the biomass of soybean shoots and roots, significantly( P <0.05), with control effect of 75.29% on *** is the first report of the control effect of *** on PRR caused by *** results mentioned above suggested that *** strain BS-4 is a novel potential biocontrol agent to control soybean root rot caused by ***, and will have a broad application prospect in plant disease *** studies provide a basis for developing *** strain BS-4 into a new biocontrol ***, the develop of BS-4 preparation and its field application need further investigation.

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DNA fragment RPA190-pc participates in regulating metlaxyl resistance in Phytophthora capsici

DNA fragment RPA190-pc participates in regulating metlaxyl r...

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中国植物病理学会2019年学术年会

作者： Wang Weiyan Liu Dong Zhuo Xin Wang Yiye Song Zhiqiang Zhang Huajian Chen Fangxin pan yuemin Gao Zhimou College of Plant Protection Anhui Agricultural University

Metalaxyl is one of the main fungicides for the control of pepper blight caused by Phytophthora *** to long-term heavy use,the resistance of *** to metalaxyl is becoming more and more *** order to reveal the molecular mechanism of *** resistance to metalaxyl,the homologous alignment analysis of the protective domain RPOLA-N of RPA190 gene in *** was conducted,and the candidate DNA fragment RPA190-pc,which may be involved in the metalaxyl resistance of ***,was *** gene was cloned and sequenced,and a conserved RPOLA-N domain was found to be contained in *** silencing converters and one over-expression convertor were obtained by using PEG mediated protoplast transformation method,and the expression of RPA190-pc in each convertor was analyzed by *** results showed that the gene silencing efficiency of SD1-9 C-3 and SD1-9 C-4 was above 60%.The gene expression efficiency of the over-expressing transformant OESD1-9-1 was three times that of the control strain *** resistance levels of these three transformants to metalaxyl were also *** results showed that the ECvalue of metalaxyl to SD1-9 C-3 was 505.096 5 μg/mL,and the EC value of M SD1-9 was decreased 24.23%,the EC value of SD1-9 C-4 was 67.035 4 μg/mL,the EC value of M SD1-9 was decreased by89.94%;and the ECvalue of OESD1-9-1 was 15 573.023 6 μg/*** ECvalue for M SD1-9 was increased by 23.26 *** above results indicate that the candidate DNA fragment RPA190-pc is involved in the regulation of *** resistance to metalaxyl.

关键词： Phytophthora capsici metalaxyl resistance gene silencing protoplast transformation

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小麦全蚀病菌拮抗细菌的筛选及生防效果

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植物保护学报 2023年第3期50卷 716-724页

作者：秦旭冯艳娟羊国根鞠玉亮杨倩潘月敏安徽农业大学植物保护学院植物病虫害生物学与绿色防控安徽普通高校重点实验室安徽省绿色农药研发与应用创新团队合肥230036

为筛选小麦全蚀病菌Gaeumannomyces tritici的拮抗细菌,从健康小麦根际土壤中分离和筛选拮抗细菌,采用对峙培养法和室内盆栽试验测定拮抗细菌对小麦全蚀病菌的抑制活性及生防效果,通过形态学特征观察、生理生化特性测定和保守序列分析... 详细信息

为筛选小麦全蚀病菌Gaeumannomyces tritici的拮抗细菌,从健康小麦根际土壤中分离和筛选拮抗细菌,采用对峙培养法和室内盆栽试验测定拮抗细菌对小麦全蚀病菌的抑制活性及生防效果,通过形态学特征观察、生理生化特性测定和保守序列分析确定其分类地位。结果表明,从小麦根际土壤中分离并筛选获得2株具有较好拮抗效果的菌株XJ-3和XJ-4,对小麦全蚀病菌菌丝生长的抑制率分别为64.31%和65.25%。基于菌株XJ-3和XJ-4的形态学特征、生理生化特性和gyrB基因系统进化分析结果,将其均鉴定为贝莱斯芽胞杆菌Bacillus velezensis。菌株XJ-3和XJ-4的发酵液中均含有一类具抗真菌活性、耐热性的非蛋白类次生代谢产物;经菌株XJ-3和XJ-4发酵液处理小麦幼苗后,株高较对照分别显著增加了6.48%和13.12%,干重较对照分别增加了1.30%和14.29%,其中菌株XJ-4的促生效果相对较好;对小麦全蚀病的盆栽防效分别达到51.29%和52.46%。表明筛选得到的贝莱斯芽胞杆菌XJ-3和XJ-4可用于小麦全蚀病的生物防治,具有开发为生防制剂的潜力。

关键词：小麦全蚀病小麦顶囊壳贝莱斯芽胞杆菌鉴定生物防治促生效果

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拔尖创新人才培养背景下植物保护专业实践教学改革与实践

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合肥师范学院学报 2016年第3期34卷 96-99页

作者：郭敏潘月敏安徽农业大学植物保护学院安徽合肥230036

围绕拔尖创新人才培养目标,阐述了安徽农业大学在植物保护专业实践教学中的具体做法,并对实践教学内容、教学方法、基地建设及考核体系等方面进行改革与优化。结果表明,学生在新的教学模式下,实践操作技能、分析问题和解决问题的能力均... 详细信息

围绕拔尖创新人才培养目标,阐述了安徽农业大学在植物保护专业实践教学中的具体做法,并对实践教学内容、教学方法、基地建设及考核体系等方面进行改革与优化。结果表明,学生在新的教学模式下,实践操作技能、分析问题和解决问题的能力均得到显著提高。

关键词：植物保护实践教学创新能力

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几丁寡糖对烟草种子萌发及有关酶活性的影响

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中国农学通报 2009年第14期25卷 138-141页

作者：沈奕高智谋王革潘月敏伍万荣安徽农业大学植物保护学院合肥230036 云南玉溪红塔集团生化研究中心云南玉溪653100

为了阐明几丁寡糖对烟草种子萌发的影响及其作用机制,从而为几丁寡糖在烟草生产上的应用提供必要的试验依据,在实验室条件下,测定了不同浓度的几丁寡糖溶液浸种处理对烟草K326种子发芽率和发芽势及有关酶活性的影响。结果表明,在0.2～1.... 详细信息

为了阐明几丁寡糖对烟草种子萌发的影响及其作用机制,从而为几丁寡糖在烟草生产上的应用提供必要的试验依据,在实验室条件下,测定了不同浓度的几丁寡糖溶液浸种处理对烟草K326种子发芽率和发芽势及有关酶活性的影响。结果表明,在0.2～1.0mg/ml浓度范围内,几丁寡糖溶液浸种处理能显著提高烟草种子发芽率和发芽势,最适浓度为0.4mg/ml;用0.4mg/ml几丁寡糖处理的烟草种子体内过氧化物酶(POD)活性显著高于对照,超氧化物歧化酶(SOD)和苯丙氨酸解氨酶(PLA)活性均显著高于不浸种对照。基于试验结果,对几丁寡糖处理对烟草种子发芽率和发芽势影响的机制进行了讨论。

关键词：几丁寡糖烟草种子萌发酶活性

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串珠镰孢对棉苗致病力分化及其在单分生孢子后代的遗传

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热带作物学报 2011年第8期32卷 1553-1556页

作者：潘月敏高智谋凌萍代玉立安徽农业大学植物保护学院安徽合肥230036

采用菌丝块创伤接种法,分别测定了不同寄主来源的串珠镰孢对棉苗叶片的致病力。结果表明,所有供试菌株接种棉苗后均可引起发病,但不同菌株所致病斑的平均直径有显著差异,提示串珠镰孢菌株间对棉苗叶片的致病力存在明显的分化,来自棉花... 详细信息

采用菌丝块创伤接种法,分别测定了不同寄主来源的串珠镰孢对棉苗叶片的致病力。结果表明,所有供试菌株接种棉苗后均可引起发病,但不同菌株所致病斑的平均直径有显著差异,提示串珠镰孢菌株间对棉苗叶片的致病力存在明显的分化,来自棉花的菌株对棉苗叶片的致病力较强,来自玉米和水稻的菌株对棉苗叶片的致病力较弱;即使来自相同寄主的菌株间致病力也存在差异,但菌株致病力差异与菌株地域来源无明显相关。以菌株Fm1、Fm19和Fm31为亲本,采用同样的接种法测定了串珠镰孢对棉苗致病力在单分生孢子后代的遗传变异。结果表明,串珠镰孢对棉苗的致病力在单分生孢子后代能连续两代稳定遗传。

关键词：棉花串珠镰孢致病力分化遗传

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小麦全蚀病菌重组酶聚合酶检测方法的建立及应用

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植物保护 2020年第5期46卷 150-155,180页

作者：鞠玉亮沈鹏飞范志朋羊国根吴迅宛宁潘月敏安徽农业大学植物保护学院植物病虫害生物学与绿色防控安徽普通高校重点实验室安徽省绿色农药研发与应用创新团队合肥230036

由禾顶囊壳小麦变种Gaeumannomyces graminis ***引起的小麦全蚀病是危害极大的小麦土传真菌病害。本研究以小麦全蚀病菌β-tubulin为靶标基因设计重组酶聚合酶扩增技术(recombinase polymerase amplification,RPA)引物Gg-RPA-F/Gg-RPA-R和RPA探针Gg-LF-Probe,结合侧流层析试纸条,建立了小麦全蚀病菌RPA快速检测方法,该方法在38℃恒温条件下30 min内完成可视化检测,摆脱PCR仪等仪器设备的限制。小麦全蚀病菌RPA快速检测方法具有较高的检测特异性,其检测极限为10 pg/μL,与普通PCR一致。此外,小麦全蚀病菌RPA快速检测方法可从土壤中快速检测到小麦全蚀病菌,具有较强的实用性。因此,本研究建立的小麦全蚀病菌RPA快速检测方法具备简便高效、实用性强的特点,为小麦全蚀病菌的快速检测和病害早期诊断提供新技术。

关键词：小麦全蚀病菌重组酶聚合酶技术特异性灵敏度实用性

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