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检验医学科专业基地住院医师规范化培训教学查房形式的探索

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中国毕业后医学教育 2024年第8期8卷 583-586页

作者：王宏刘根焰徐建潘世扬南京医科大学第一附属医院检验学部江苏南京210029

教学查房是住院医师规范化培训实践教学的重要环节,是培养住院医师临床能力的有效途径。检验医学具有鲜明的独立特性,因此检验医学科专业基地与临床类专业基地相比,教学查房形式有相同点也有其特色。为了更好地提升检验医学科住院医师... 详细信息

教学查房是住院医师规范化培训实践教学的重要环节,是培养住院医师临床能力的有效途径。检验医学具有鲜明的独立特性,因此检验医学科专业基地与临床类专业基地相比,教学查房形式有相同点也有其特色。为了更好地提升检验医学科住院医师核心胜任力,南京医科大学第一附属医院检验医学科专业基地探索了既可以达到教学目标又具有检验医学特点的教学查房形式。

关键词：检验医学科住院医师教学查房住院医师规范化培训检验医学核心胜任力

爪式真空泵新型齿轮爪式转子及其力学性能研究

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真空科学与技术学报 2024年第7期44卷 583-591页

作者：李杰杰任纯吉王君李影潘诗洋胡金月李子珍崔冬中国石油大学(华东)新能源学院青岛266580 高端压缩机及系统技术全国重点实验室合肥通用机械研究院有限公司合肥230031

爪式转子的型线设计是决定爪式真空泵性能的关键因素。齿轮爪式转子采用新型曲折型啮合结构,显著降低了传统曲爪转子存在的转子间泄漏,然而齿轮爪式转子型线上仍存在多个不光滑点,降低了力学性能。针对这一问题,文章提出了一种新型高密... 详细信息

爪式转子的型线设计是决定爪式真空泵性能的关键因素。齿轮爪式转子采用新型曲折型啮合结构,显著降低了传统曲爪转子存在的转子间泄漏,然而齿轮爪式转子型线上仍存在多个不光滑点,降低了力学性能。针对这一问题,文章提出了一种新型高密封椭圆弧型齿轮爪式转子,推导了该转子型线参数方程;分析了型线几何参数对爪泵相对余隙和容积利用率的影响,并与现有齿轮爪式转子进行对比;研究了新型转子的工作过程,对比分析了两种转子混合过程的余隙变化;对新型转子和现有齿轮爪式转子在工作过程中的应力和变形进行对比研究。研究结果表明:与现有齿轮爪式转子相比,新型转子的相对余隙减少10%以上,混合过程中的过压缩腔和膨胀腔减少,新型转子的吸气过程和混合过程中最大变形量分别减少10.11%和16.06%,极大改善了转子的性能。

关键词：高密封型爪式转子型线构建几何性能工作过程受力分析

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超细地质样品的谱学特征及矿物分布形态研究

中国无机分析化学 2024年第9期14卷 1299-1305页

作者：魏均启董学林向兆朱丹童铄云陈国超刘爽王芳潘诗洋鲁力湖北省地质实验测试中心自然资源部稀土稀有稀散矿产重点实验室武汉430034 湖北省地质局资源与生态环境地质湖北省重点实验室武汉430034 南阳理工学院土木工程学院河南南阳473000

地质样品粒度已成为制约提高分析精度的关键因素,近年来超细地质样品的制备已越来越多地受到地质分析实验室关注,目前仍广泛采用74μm的地质样品粒度水平已越来越不能满足高精度、高灵敏度、小取样量和微区现代分析技术的要求,迫切需要... 详细信息

地质样品粒度已成为制约提高分析精度的关键因素,近年来超细地质样品的制备已越来越多地受到地质分析实验室关注,目前仍广泛采用74μm的地质样品粒度水平已越来越不能满足高精度、高灵敏度、小取样量和微区现代分析技术的要求,迫切需要发展适应地质实验室批量生产的超细地质样品制备技术。采用X射线粉晶衍射、扫描电子显微镜、差热分析等测试技术获得了地质样品超细化前后的谱学特征、并对超细地质样品中矿物分布形态进行研究。结果表明:超细加工使矿物结晶度降低,晶体产生晶胞畸变;矿物粒度分布主要集中于0.5~30μm,随矿物粒径减小,颗粒棱角逐渐消失,颗粒边界变模糊;矿物超细化后脱吸附水的热效应范围变宽,吸热效应变宽缓;矿物在超细地质样品中有单颗粒、集合体、团聚、包裹四种分布形态,影响地质样品超细化程度的主要因素是不易粉碎的矿物单颗粒和矿物集合体。基本查明了超细地质样品的谱学特征和矿物形态分布特点,为改进超细地质样品制备工艺和开发先进制备技术提供科学依据,对解决地质样品超细化均质性问题,提高地质样品分析准确性具有重要意义。

关键词：超细地质样品 X射线粉晶衍射扫描电子显微镜差热分析矿物形态分布

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新一代测序技术在肺癌个体化医疗中的应用

中华检验医学杂志 2019年第2期42卷 77-83页

作者：潘世扬南京医科大学第一附属医院江苏省人民医院检验学部南京210029

肺癌是全球最常见的恶性肿瘤,死亡率居癌症之首。肺癌与其他肿瘤一样,在肿瘤的发生发展过程中存在异质性。对异质性的全面了解才能实现真正意义上的个体化治疗,这也是精准医疗的精髓。近年来,新一代测序技术依托其高通量、多靶点分析的... 详细信息

肺癌是全球最常见的恶性肿瘤,死亡率居癌症之首。肺癌与其他肿瘤一样,在肿瘤的发生发展过程中存在异质性。对异质性的全面了解才能实现真正意义上的个体化治疗,这也是精准医疗的精髓。近年来,新一代测序技术依托其高通量、多靶点分析的优势为精准医疗提供了新的重要技术手段,在肺癌的分子分型、靶向药物选择及预后判断中发挥着越来越重要的作用。

关键词：肺肿瘤高通量核苷酸序列分析精准医学

Activation of calcium-sensing receptors is associated with apoptosis in a model of simulated cardiomyocytes ischemia/reperfusion

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The Journal of Biomedical Research 2010年第4期24卷 301-307页

作者： Ling Yan Tlebing Zhu Tingting Sun Liansheng Wang shiyang pan Zhengxlan Tao Zhijian Yang Kejiang Cao Department of Cardiology the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 Jiangsu Province China Department of Clinical Key Laboratory the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 Jiangsu Province China Department of Biochemistry Nanjing Medical University Nanjing 210029 Jiangsu Province China

Objective： Calcium-sensing receptors （CaSRs） are G-protein coupled receptors which maintain systemic calcium homeostasis and participate in hormone secretion, activation of ion channels, cell apoptosis, proliferation, and differentiation. Previous studies have shown that CaSRs induce apoptosis in isolated adult rat heart and in normal neonatal rat cardiomyocytes by G-protein-PLC-IP3 signaling transduction. However, little knowledge is presently available concerning the role of CaSRs in the apoptosis induced by ischemia and reperfusion in neonatal cardiomyocytes. Methods： Primary neonatal rat ventricular cardiomyocytes were incubated in ischemiamimetic solution for 2 h, and then re-incubated in normal culture medium for 24 h to establish a model of simu- lated ischemia/reperfusion （I/R）. Cardiomyocyte apoptosis was detected by terminal deoxynucleotidyl transferase- mediated dUTP nick end labeling （TUNEL）. The expression of CaSRs mRNA was detected by real-time reverse transcription polymerase chain reaction （RT-PCR）. In addition, the expressions of caspase-3 and Bcl-2 were analyzed by western blot. Results： The simulated I/R enhanced the expression of CaSRs and cardiomyocyte apoptosis. GdCl3, a specific activator of CaSRs, further increased the expression of CaSRs and cardiomyocyte apoptosis, along with up-regulation of caspase-3 and down-regulation of Bcl-2. Conclusion： CaSRs are associated with UR injury and apoptosis in neonatal rat ventricular cardiomyocytes via suppressing Bcl-2 and promoting caspase-3 expression.

关键词： calcium sensing receptors apoptosis cardiomyocyte ischemia/reperfusion

Prevalence and trends of aminoglycoside resistance in Shigella worldwide, 1999-2010

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The Journal of Biomedical Research 2013年第2期27卷 103-115页

作者： Bing Gu Xing Ke shiyang pan Yan Cao Ling Zhuang Rongbin Yu Huimin Qian Genyan Liu Mingqing Tong Department of Laboratory Medicine the First Affiliated Hospital Nanjing Medical University National Key Clinical Department of Laboratory Medicine Department of Acute Infectious Disease Prevention and Control Jiangsu Provincial Center for Disease Prevention and Control Department of Epidemiology and Biostatistics School of Public Health Nanjing Medical University

Shigellosis causes diarrheal disease in humans in both developed and developing countries, and multi-drug resistance in Shigella is an emerging problem. Understanding changing resistance patterns is important in determining appropriate antibiotic treatments. This meta-analysis systematically evaluated aminoglycoside resistance in Shigella. A systematic review was constructed based on MEDLINE and EMBASE databases. Randomeffect models or fixed-effect models were used based on P value considering the possibility of heterogeneity between studies for meta-analysis. Data manipulation and statistical analyses were performed using software STATA 11.0. By means of meta-analysis, we found a lower resistance to three kinds of aminoglycosides in the Europe-America areas during the 12 year study period than that of the Asia-Africa areas. Kanamycin resistance was observed to be the most common drug resistance among Shigella isolates with a prevalence of 6.88% （95%CI： 6.36%-7.43%）. Comparison of data from Europe-America and Asia-Africa areas revealed that Shigella flexneri resistance was greater than the resistance calculated for Shigella sonnei. Importantly, Shigella sonnei has played a significant role in aminoglycoside-resistance in recent years. Similarly, data showed that resistance to these drugs in children was higher than the corresponding data of adults. In conclusion, aminoglycoside-resistant Shigella is not an unusual phenomenon worldwide. Distribution in Shigella resistance differs sharply based on geographic areas, periods of time and subtypes. The results from the present study highlight the need for con- tinuous surveillance of resistance and control of antibiotic usage.

关键词： Shigella aminoglycoside resistance patterns prevalence trends meta-analysis

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肺癌疗效评价标志物的研究进展

山东大学学报（医学版） 2018年第10期56卷 31-39,45页

作者：潘世扬江苏省人民医院南京医科大学第一附属医院检验科江苏南京210029

肺癌是全球最常见的恶性肿瘤,也是癌症相关死亡的首位死因。晚期肺癌治疗主要是采用以化疗为主的综合疗法。目前主要依靠胸片等影像学检查定期进行化疗疗效评判,缺乏足够的特异性与敏感性,难以真正实现个体化治疗。因此寻找高敏感度的... 详细信息

肺癌是全球最常见的恶性肿瘤,也是癌症相关死亡的首位死因。晚期肺癌治疗主要是采用以化疗为主的综合疗法。目前主要依靠胸片等影像学检查定期进行化疗疗效评判,缺乏足够的特异性与敏感性,难以真正实现个体化治疗。因此寻找高敏感度的疗效考核指标就显得尤为重要。对目前临床应用肺癌治疗疗效评价的影像学和血液学标志物研究进展进行简要阐述。

关键词：肺癌化疗疗效标志物

Class Ⅰ integron with a novel cassette array in an ESBL-producing multidrug-resistant Klebsiella pneumoniae isolate

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Journal of Nanjing Medical University 2006年第1期20卷 12-16页

作者： Bing Gu Mingqing Tong Wangsheng Zhao shiyang pan Yuanhua Wei Peijun Huang Department of Clinical Laboratories the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China

Objective： To analyze the molecular mechanism of integron mediated mulfi-resistanc, e in an ESBL-producing K. pneumoniae NJ 12 isolate. Methods： Susceptibility test was carried out by Kirby-Bauer method. Class Ⅰ, Ⅱ and Ⅲ integrons were detected by integrase gene PCR with primers that annealed to conserved regions of integron-encoded integrase genes intll, intl2 and intl3. The variable region of integron was amplified by integron PCR with primers that targeted the conserved flanking regions, and the product was sequenced. Six aminoglycoside modifying-enzyme genes, including ant（2＂）-Ⅰ, ant（3＂）- Ⅰ, aac（3）- Ⅰ, aac（3）-Ⅱ, aac （6＇）-Ⅰ, and aac（6＇）-Ⅱ , were detected. Results： K. pneumoniae NJ 12 was resistant to nine antibiotics, including piperacillin, ampicillin, cefuroxime, ceftazidime, cefotaxime, aztreonam, streptomycin, gentamicin and amikacin. This isolate was shown that there was positive with class Ⅰ integron, ant（2＂）- Ⅰ , ant（3＂）- Ⅰ , aac（3）-Ⅱ and aac（6＇）- Ⅰ modifying-enzyme genes. Neither class Ⅱ nor Ⅲ integron was detected; DNA sequencing of the fragment amplified by integron PCR revealed a novel cassette array aadR-cat-blaoxa-10/ aadA1. Conclusion： Class I integron with a novel cassette array in an ESBL-producing multidrug-resistant K. pneumon/ae NJ 12 isolate was reported from Nanjing area of China, with the GenBank accession number DQ141319.

关键词： integron gene cassette multi-resistance K.pneumoniae ESBL

Comparison of prevalence and changing resistance to nalidixic acid and ciprofloxacin of Shigella between Europe-America and Asia-Africa,1998-2009

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Comparison of prevalence and changing resistance to nalidixi...