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Assessment of the binding interactions of SARS-CoV-2 spike glycoprotein variants

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Journal of Pharmaceutical Analysis 2022年第1期12卷 58-64页

作者： Deepa Raghu pamela Hamill Arpitha Banaji Amy McLaren Yu-Ting Hsu Product Characterization MilliporeSigmaRockvilleMD20850USA Product Characterization MilliporeSigmaStirlingFK94NFUK Process and Analytical Development MilliporeSigmaSt.LouisMO63118USA

Severe acute respiratory syndrome-associated coronavirus 2 is a major global health issue and is driving the need for new *** surface spike protein,which plays a central role in virus infection,is currently the target for vaccines and neutralizing *** emergence of novel variants with multiple mutations in the spike protein may reduce the effectiveness of neutralizing antibodies by altering the binding activity of the protein with angiotensin-converting enzyme 2(ACE2).To understand the impact of spike protein mutations on the binding interactions required for virus infection and the effectiveness of neutralizing monoclonal antibody(mAb)therapies,the binding activities of the original spike protein receptor binding domain(RBD)sequence and the reported spike protein variants were investigated using surface plasmon *** addition,the interactions of the ACE2 receptor,an antispike mAb(mAb1),a neutralizing mAb(mAb2),the original spike RBD sequence,and mutants D614G,N501Y,N439K,Y453F,and E484K were *** to the original RBD,the Y453F and N501Y mutants displayed a significant increase in ACE2 binding affinity,whereas D614G had a substantial reduction in binding *** mAb-RBD mutant proteins displayed a reduction in binding affinities relative to the original RBD,except for the E484K-mAb1 *** potential neutralizing capability of mAb1 and mAb2 was ***,mAb1 failed to inhibit the ACE2-RBD interaction while mAb2 inhibited the ACE2-RBD interactions for all RBD mutants,except mutant E484K,which only displayed partial blocking.

关键词： Surface plasmon resonance SARS-CoV-2 Monoclonal antibody RBD ACE2

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International Journal of Oral Science 2013年第1期5卷 1-6页

作者： Li-Wei Zheng Logan Linthicum pamela K DenBesten Yan Zhang Department of Orofacial Sciences University of California Department of Biology Humboldt State University

This study aimed to compare epithelial cells derived from human embryonic stem cells(hESCs) to human ameloblast-lineage cells (ALCs),as a way to determine their potential use as a cell source for ameloblast *** by various concentrations of bone morphogenetic protein 4(BMP4),retinoic acid(RA) and lithium chloride(LiCI) for 7 days,hESCs adopted cobble-stone epithelial phenotype(hESC-derived epithelial cells(ES-ECs)) and expressed cytokeratin *** with ALCs and oral epithelial cells(OE), ES-ECs expressed amelogenesis-associated genes similar to ***-ECs were compared with human fetal skin epithelium,human fetal oral buccal mucosal epithelial cells and human ALCs for their expression pattern of cytokeratins as *** had relatively high expression levels of cytokeratin 76,which was also found to be upregulated in *** on the present study,with the similarity of gene expression with ALCs,ES-ECs are a promising potential cell source for regeneration,which are not available in erupted human teeth for regeneration of enamel.

关键词： ameloblast cytokeratin dental epithelial cells human embryonic stem cells odontogenesis

Potential distributional shifts in North America of allelopathic invasive plant species under climate change models

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Plant Diversity 2022年第1期44卷 11-19页

作者： Anson Wang Anthony E.Melton Douglas ESoltis pamela SSoltis Department of Biology University of FloridaGainesvilleFL32611USA Florida Museum of Natural History University of FloridaGainesvilleFL32611USA Genetics Institute University of FloridaGainesvilleFL32610USA Biodiversity Institute University of FloridaGainesvilleFL32611USA

Predictive studies play a crucial role in the study of biological invasions of terrestrial plants under possible climate change *** species are recognized for their ability to modify soil microbial communities and influence ecosystem ***,we focused on six species of allelopathic flowering plants-Ailanthus altissima,Casuarina equisetifolia,Centaurea stoebe ***,Dioscorea bulbifera,Lantana camara,and Schinus terebinthifolia-Xhat are invasive in North America and examined their potential to spread further during projected climate *** used Species Distribution Models(SDMs)to predict future suitable areas for these species in North America under several proposed future climate *** and Maxent were used to develop SDMs,estimate current distributions,and predict future areas of suitable climate for each *** with the greatest predicted suitable climate in the future include the northeastern and the coastal northwestern regions of North *** size estimations demonstrate the possibility of extreme range loss for these invasives in the southeastern United States,while new areas may become suitable in the northeastern United States and southeastern *** findings show an overall northward shift of suitable climate during the next few decades,given projected changes in temperature and *** results can be utilized to analyze potential shifts in the distribution of these invasive species and may aid in the development of conservation and management plans to target and control dissemination in areas at higher risk for potential future invasion by these allelopathic species.

关键词： Allelopathy Invasive species Species distribution models Ecological niche models Invasion impacts Multi-species assessment

Recent updates on the biological basis of heterogeneity in bone marrow stromal cells/ skeletal stem cells

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Biomaterials Translational 2022年第1期3卷 3-16页

作者： Deepika Arora pamela Gehron Robey National Institute of Dental and Craniofacial Research National Institutes of HealthDepartment of Health and Human ServicesBethesdaMDUSA Biosystems and Biomaterials Division National Institute of Standards and TechnologyDepartment of CommerceGaithersburgMDUSA Department of Biotechnology School of Biological Engineering&Life SciencesShobhit Institute of Engineering&Technology(Deemed-to-be-University)MeerutIndia

Based on studies over the last several decades,the self-renewing skeletal lineages derived from bone marrow stroma could be an ideal source for skeletal tissue ***,the markers for osteogenic precursors;i.e.,bone marrow-derived skeletal stem cells(SSCs),in association with other cells of the marrow stroma(bone marrow stromal cells,BMSCs)and their heterogeneous nature both in vivo and in vitro remain to be *** review aims to highlight:i)the importance of distinguishing BMSCs/SSCs from other“mesenchymal stem/stromal cells”,and ii)factors that are responsible for their heterogeneity,and how these factors impact on the differentiation potential of SSCs towards *** prospective role of SSC enrichment,their expansion and its impact on SSC phenotype is *** has also been given to emerging single cell RNA sequencing approaches in scrutinizing the unique population of SSCs within the BMSC population,along with their committed *** the factors involved in heterogeneity may help researchers to improvise their strategies to isolate,characterize and adopt best culture practices and source identification to develop standard operating protocols for developing reproducible stem cells ***,more scientific understanding of the molecular basis of heterogeneity is warranted that may be obtained from the robust high-throughput functional transcriptomics of single cells or clonal populations.

关键词： bone marrow stromal cells clonal analysis heterogeneity single cell analysis skeletal stem cells

Role of chromosome 1q copy number variation in hepatocellular carcinoma

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World Journal of Hepatology 2021年第6期13卷 662-672页

作者： Nathan R Jacobs pamela A Norton Department of Microbiology and Immunology Drexel University College of MedicinePhiladelphiaPA 19102United States

Chromosome 1q often has been observed to be amplified in hepatocellular *** review summarizes literature reports of multiple genes that have been proposed as possible 1q amplification *** largely fall within *** addition,publicly available copy number alteration data from The Cancer Genome Atlas project were used to identify additional candidate genes involved in *** most frequent location for gene amplification was 1q22,consistent with the results of the literature *** genes TPM3 and NUF2 were found to be candidates whose amplification and/or mRNA up-regulation was most highly associated with poorer hepatocellular carcinoma outcomes.

关键词： Liver cancer Chromosomal amplification Hepatocellular carcinoma The Cancer Genome Atlas

Hericium Erinaceus (Bull.:Fr.) Pers.,A Medicinal Mushroom,Activates Peripheral Nerve Regeneration

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Chinese Journal of Integrative Medicine 2016年第10期22卷 759-767页

作者： Kah-Hui Wong Gowri Kanagasabapathy Murali Naidu pamela David Vikineswary Sabaratnam Department of Anatomy Faculty of Medicine University of Malaya Mushroom Research Centre University of Malaya Institute of Biological Sciences Faculty of Science University of Malaya

Objective： To study the ability of aqueous extract of Hericium erinaceus mushroom in the treatment of nerve injury following peroneal nerve crush in Sprague-Dawley rats. Methods： Aqueous extract of Hericium erinaceus was given by daily oral administration following peroneal nerve crush injury in Sprague- Dawley rats. The expression of protein kinase B （Akt） and mitogen-activated protein kinase （MAPK） signaling pathways; and c-Jun and c-Fos genes were studied in dorsal root ganglia （DRG） whereas the activity of protein synthesis was assessed in peroneal nerves by immunohistochemical method. Results： Peripheral nerve injury leads to changes at the axonal site of injury and remotely located DRG containing cell bodies of sensory afferent neurons. Immunofluorescence studies showed that DRG neurons ipsilateral to the crush injury in rats of treated groups expressed higher immunoreactivities for Ak＇t, MAPK, c-Jun and c-Fos as compared with negative control group （P〈0.05）. The intensity of nuclear ribonucleoprotein in the distal segments of crushed nerves of treated groups was significantly higher than in the negative control group （P〈0.05）. Conclusion： H. erinaceus is capable of promoting peripheral nerve regeneration after injury. Potential signaling pathways include Akt, MAPK, c-Jun, and c-Fos, and protein synthesis have been shown to be involved in its action.

关键词： Hericium erinaceus dorsal root ganglia signaling pathways gene expression protein synthesis machinery peripheral nerve regeneration

A Study on Strategies to Retain and Motivate Employees in the Ministry of Education in Zambia

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A Study on Strategies to Retain and Motivate Employees in th...

作者： pamela Nachona Naluyele 华中科技大学

学位级别：硕士

对教育工作者的裁减与自然调整已在全球成为了一种普遍现象。这种人员的调整造成了教育工作者数量短缺,导致了用人单位招聘成本的增加。而对教育人工作员与受雇佣者的管理与限制导致了教育培训的计划的连续性与阶段性受限。本文的研究... 详细信息

对教育工作者的裁减与自然调整已在全球成为了一种普遍现象。这种人员的调整造成了教育工作者数量短缺,导致了用人单位招聘成本的增加。而对教育人工作员与受雇佣者的管理与限制导致了教育培训的计划的连续性与阶段性受限。本文的研究目的就是评估赞比亚的为留住雇员继续在教育部门（MOE）任职的策略。一国对教育工作者的调整与挽留策略在人员的裁减过程中至关重要,因为赞比亚教育部一直致力于为全体国民提供最优秀的教育。本国教育的目的是为个人、社会、经济发展服务,并逐步改善人民的生活质量。对教师以及其他教育从业人员的管理需要教育系统各利益相关者的齐心协力,而且教育管理部门占有举足轻重的作用,但现实是教育人员在学校中的流动率居高不下。教师的调整应该是全局性的、双边的,更为重要的是学校管理者应具备能够影响学校管理的影响力,以促进教师的工作和奉献精神的履行。招聘工作同样重要,应吸引留学人员为国家社会经济运行服务,并努力把他们安排到基层与地方进行教学。对雇员成就的认可是激励员工最重要的手段之一。几十年来,在公众与私人机构奖励和表扬的手段已经广泛存在,受雇者也会以各种形式受到表彰。此外,由于个体独特性的存在,同一种奖励机制对每个人的激励作用也是不同的,对一个人的表彰可能被他人视为惩罚。本研究将500份调查问卷发给了赞比亚国家级、地方级教育部门的436雇佣者。该研究还分析员工如何看待政府（雇主）提供激励和激励方面的做法、员工的优秀表现应该如何在组织中被发现、以及什么动因会促使他们继续为组织服务。文章的研究成果可以为管理者、决策者和人力资源管理部门提供参考,使得赞比亚教育部门的可以制定出适当的、高效的、成功的、奖励机制。

关键词：赞比亚教育部门挽留动因奖励

Impact of Colonization on Language Policy in Cameroon

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Impact of Colonization on Language Policy in Cameroon

作者： Ngueguo Sandra pamela 湖南大学

学位级别：硕士

喀麦隆的语言政策受到了殖民化的巨大影响。这种影响至今依然存在。喀麦隆的官方语言,英语和法语,是殖民化的遗留产物。喀麦隆作为一个多语言国家,有250多种地方语言和2种通用语。即喀麦隆洋泾浜英语和英法混合语言(Camfranglais)。除... 详细信息

喀麦隆的语言政策受到了殖民化的巨大影响。这种影响至今依然存在。喀麦隆的官方语言,英语和法语,是殖民化的遗留产物。喀麦隆作为一个多语言国家,有250多种地方语言和2种通用语。即喀麦隆洋泾浜英语和英法混合语言(Camfranglais)。除官方双语外,其他语言都被归入第二计划,使得文化传承受到影响,许多语言正走向消亡。本研究采用定量研究方法,采用在线问卷调查的方式,调查了喀麦隆英语区和法语区的受访者112人,就殖民化对语言政策的影响和对当地和官方语言未来的发展看法,问卷共设26个问题。本研究通过谷歌平台进行问卷调查,以便喀麦隆受访者参与。结果显示,殖民化对喀麦隆语言政策的影响既有正面的,也有负面的。我们发现,在官方的外国双语氛围中,年轻人在学校或家里通过这些语言接触到更多的外国文化,更倾向于外国文化,致使当地的多语制形同虚设。我们还发现,由外国语言和当地语言结合而成的混合语言在喀麦隆获得了很大发展,特别是在年轻人中间,尽管这些语言还没有被正式获得官方语言政策的确定和支持。受殖民主义的影响,喀麦隆的语言氛围存在冲突,官方双语很大程度上主导了当地的多语制,忽视了当地语言,导致文化逐渐消亡。殖民化对喀麦隆语言政策最严重的影响是该国当前所谓的英语危机或安巴索尼亚战争。本研究表明,由于殖民主义对喀麦隆语言政策的长期影响,实施可持续的语言政策迫在眉睫。本研究对于保护喀麦隆当地语言、避免语言消亡从而促进国家文化和民族的团结统一具有重要的意义。

关键词：殖民化喀麦隆语言政策地方语言官方语言

The Values and Significance of the Colonial Steel Railway Bridges of Pakistan

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Built Heritage 2017年第4期1卷 11-21页

作者： Ayesha pamela Rogers Ghulam Mustafa Baloch Cultural Studies Department National College of ArtsLahorePakistan

This paper proposes that Civil Engineering Heritage is a distinct form of heritage,related to but differing from Industrial Heritage and a separate field that should be treated *** critical element that signifies engineering heritage is ingenium or innovation in development of complex technological responses to physical *** heritage requires specific approaches to its identification,documentation,conservation and *** important first step of this process is a clear understanding of the values and attributes that contribute to the significance of engineering heritage and must be *** example of engineering heritage examined here is the colonial steel railway bridges of Pakistan;three case study bridges are presented and the values and attributes of each are *** paper then identifies a set of shared essential values of this type of engineering heritage that can serve as a basis for value-based conservation and management.

关键词： civil engineering heritage bridges colonial Pakistan values innovation